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Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Wolinella succinogenes

NCTC Number: NCTC 11488
Current Name: Wolinella succinogenes
Original Strain Reference: FDC 602
Other Collection No: ATCC 29543; DSM 1740
Previous Catalogue Name: Wolinella succinogenes
Type Strain: Yes
Family: Helicobacteraceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Fastidious anaerobe agar, 48 hours, 37°C, anaerobic
Conditions for growth on liquid media: nutrient broth,37, strict anaerobe
Isolated From: bovine rumen in 1960
Whole Genome Sequence:
16S rRNA Gene Sequence: >gb|Z25745|NCTC 11488 (NCTC TYPE STRAIN)|W.succinogenes gene for 16S ribosomal RNA (partial).| caacggcttaaccgt... >gb|M88159|ATCC 29543|Wolinella succinogenes strain ATCC 29543 16S ribosomal RNA, partialsequence.| ttatggagagtttga...
23S rRNA Gene Sequence: >gb|AY596228|ATCC 29543|Wolinella succinogenes strain ATCC 29543 23S ribosomal RNA gene,partial sequence.| aaataagcttttaag...
Bibliography: TANNERA C R ET AL 1981 INT J SYST BACT 31 432 445
Extended Bibliography: showhide Show bibliography
Ref #: 95515
Author(s): Zakharova,N.;Paster,B.J.;Wesley,I.;Dewhirst,F.E.;Berg,D.E.;Severinov,K.V.
Journal: J Bacteriol
Title: Fused and overlapping rpoB and rpoC genes in Helicobacters, Campylobacters, and related bacteria
Volume: 181
Page(s): 3857-9
Year: 1999
Keyword(s): GENBANK/AF136503 GENBANK/AF136504 GENBANK/AF136505 GENBANK/AF136506 GENBANK/AF136507 GENBANK/AF136508 GENBANK/AF136509 GENBANK/AF136510 GENBANK/AF136511 GENBANK/AF136512 GENBANK/AF136513 GENBANK/AF136514 GENBANK/AF136515 GENBANK/AF136516 GENBANK/AF136517 GENBANK/AF136518 Amino Acid Sequence Base Sequence Campylobacter/classification/*genetics/isolation & purification DNA-Directed RNA Polymerases/chemistry/*genetics *Evolution, Molecular Gastric Mucosa/microbiology Genes, Bacterial Genes, Overlapping Gram-Negative Bacteria/genetics Helicobacter/classification/*genetics/isolation & purification Humans Molecular Sequence Data *Phylogeny Sequence Alignment Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Wolinella/genetics
Remarks: The genes coding for the beta (rpoB) and beta' (rpoC) subunits of RNA polymerase are fused in the gastric pathogen Helicobacter pylori but separate in other taxonomic groups. To better understand how the unique fused structure evolved, we determined DNA sequences at and around the rpoB-rpoC junction in 10 gastric and nongastric species of Helicobacter and in members of the related genera Wolinella, Arcobacter, Sulfurospirillum, and Campylobacter. We found the fusion to be specific to Helicobacter and Wolinella genera; rpoB and rpoC overlap in the other genera. The fusion may have arisen by a frameshift mutation at the site of rpoB and rpoC overlap. Loss of good Shine-Dalgarno sequences might then have fixed the fusion in the Helicobacteraceae, even if fusion itself did not confer a selective advantage.
URL: 10368167
Ref #: 20722
Author(s): Wesley,I.V.;Schroeder-Tucker,L.;Baetz,A.L.;Dewhirst,F.E.;Paster,B.J.
Journal: J Clin Microbiol
Title: Arcobacter-specific and Arcobacter butzleri-specific 16S rRNA-based DNA probes
Volume: 33
Page(s): 1691-8
Year: 1995
Keyword(s): GENBANK/L04312 GENBANK/L04313 GENBANK/L04314 GENBANK/L04315 GENBANK/L04316 GENBANK/L04317 GENBANK/L04318 GENBANK/L04319 GENBANK/L04320 GENBANK/L04321 GENBANK/L04322 GENBANK/L06974 GENBANK/L06978 GENBANK/L14625 GENBANK/L14627 GENBANK/L14628 GENBANK/L14630 GENBANK/L14631 GENBANK/M35048 GENBANK/M65010 GENBANK/M88157 GENBANK/M88159 GENBANK/U25805 Animals Base Sequence Campylobacter/classification/*genetics/pathogenicity DNA Probes/*genetics Enteritis/microbiology Female Gram-Negative Bacteria/classification/*genetics/pathogenicity Gram-Negative Bacterial Infections/microbiology/veterinary Helicobacter/classification/genetics Horse Diseases/microbiology Horses Humans Male Molecular Sequence Data Phylogeny Pregnancy RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Species Specificity Swine Swine Diseases/microbiology
Remarks: The genus Arcobacter encompasses gram-negative, aerotolerant, spiral-shaped bacteria formerly designated Campylobacter cryaerophila. Two genus-specific 16S rRNA-based oligonucleotide DNA probes (23-mer and 27-mer) were developed. The probes hybridized with strains of Arcobacter butzleri (n = 58), Arcobacter cryaerophilus (n = 19), and Arcobacter skirrowii (n = 17). The probes did not cross-react with any of the reference strains of Campylobacter, Helicobacter, including "Flexispira rappini," or Wolinella. The 27-mer hybridized with 61 Arcobacter spp. field isolates originating from late-term aborted porcine (n = 54) and equine (n = 2) fetuses and humans with enteritis (n = 5). The species of Arcobacter isolates (n = 56) recovered from aborted livestock fetuses were determined by ribotyping and were as follows: A. cryaerophilus group 1A (11 of 56; 20%), A. cryaerophilus group 1B (37 of 56; 66%), A. butzleri (5 of 56; 9%), and unknown (3 of 56; 5%). The five human field strains were identified as A. butzleri. A species-specific DNA probe (24-mer) for A. butzleri was also developed since there is evidence that this organism may be a human pathogen. This probe hybridized with previously characterized strains of A. butzleri (n = 58), with 10 field strains identified as A. butzleri by ribotyping and with 2 strains having an indeterminate ribotype. The A. butzleri-specific probe did not cross-react with strains of A. skirrowii (n = 17) and A. cryaerophilus (n = 19).
URL: 7545177
Ref #: 50052
Author(s): Dewhirst,F.E.;Shen,Z.;Scimeca,M.S.;Stokes,L.N.;Boumenna,T.;Chen,T.;Paster,B.J.;Fox,J.G.
Journal: J Bacteriol
Title: Discordant 16S and 23S rRNA gene phylogenies for the genus Helicobacter: implications for phylogenetic inference and systematics
Volume: 187
Page(s): 6106-18
Year: 2005
Keyword(s): Base Sequence DNA Primers Helicobacter/*classification/*genetics Phylogeny Polymerase Chain Reaction RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics
Remarks: Analysis of 16S rRNA gene sequences has become the primary method for determining prokaryotic phylogeny. Phylogeny is currently the basis for prokaryotic systematics. Therefore, the validity of 16S rRNA gene-based phylogenetic analyses is of fundamental importance for prokaryotic systematics. Discrepancies between 16S rRNA gene analyses and DNA-DNA hybridization and phenotypic analyses have been noted in the genus Helicobacter. To clarify these discrepancies, we sequenced the 23S rRNA genes for 55 helicobacter strains representing 41 taxa (>2,700 bases per sequence). Phylogenetic-tree construction using neighbor-joining, parsimony, and maximum likelihood methods for 23S rRNA gene sequence data yielded stable trees which were consistent with other phenotypic and genotypic methods. The 16S rRNA gene sequence-derived trees were discordant with the 23S rRNA gene trees and other data. Discrepant 16S rRNA gene sequence data for the helicobacters are consistent with the horizontal transfer of 16S rRNA gene fragments and the creation of mosaic molecules with loss of phylogenetic information. These results suggest that taxonomic decisions must be supported by other phylogenetically informative macromolecules, such as the 23S rRNA gene, when 16S rRNA gene-derived phylogeny is discordant with other credible phenotypic and genotypic methods. This study found Wolinella succinogenes to branch with the unsheathed-flagellum cluster of helicobacters by 23S rRNA gene analyses and whole-genome comparisons. This study also found intervening sequences (IVSs) in the 23S rRNA genes of strains of 12 Helicobacter species. IVSs were found in helices 10, 25, and 45, as well as between helices 31' and 27'. Simultaneous insertion of IVSs at three sites was found in H. mesocricetorum.
URL: 16109952
Data: (ATCC 29543) Type strain / A. C. R. Tanner, Boston in 1982 / Bovine rumen in 1960 / Tanner, A. C. R. et al. (1981) Int. J. syst. Bact. 31, 432-445
Accession Date: 01/01/1982
Authority: (Wolin et al. 1961) Tanner et al. 1981
Depositor: TANNER A C R
Taxonomy: TaxLink: S3584 (Wolinella succinogenes (Wolin et al. 1961) Tanner et al. 1981) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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