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Bacteria Collection: Yersinia kristensenii

NCTC Number: NCTC 11471
Current Name: Yersinia kristensenii
Original Strain Reference: 105; CL 99/82
Other Collection No: ATCC 33638; CCUG 11294; CCUG 8241; CIP 80.30; DSM 18543; JCM 7576
Previous Catalogue Name: Yersinia kristensenii
Type Strain: Yes
Family: Enterobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: not recorded
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS473433
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
Bibliography: BERCOBIER H ET AL 1980 CURRENT MICROBIOLOGY 4 210 224
Extended Bibliography: showhide Show bibliography
Ref #: 95003
Author(s): Cocolin,L.;Comi,G.
Journal: Int J Food Microbiol
Title: Use of a culture-independent molecular method to study the ecology of Yersinia spp. in food
Volume: 105
Page(s): 71-82
Year: 2005
Keyword(s): Colony Count, Microbial/methods DNA, Bacterial/*analysis Electrophoresis, Agar Gel/methods Food Contamination/*analysis Food Microbiology Polymerase Chain Reaction/*methods Serotyping Species Specificity Vegetables/*microbiology Yersinia/*isolation & purification
Remarks: A culture-independent method for the direct detection in food of Yersinia spp. was developed in this study. It is based on the amplification of a 359 bp PCR product from the RNA polymerase beta-subunit gene (rpoB) and subsequent analysis by denaturing gradient gel electrophoresis (DGGE). Direct detection of Yersinia spp. by PCR-DGGE was carried out in ready-to-eat vegetables and the results compared with the results of the traditional, culture-dependent method. The DGGE profiles were determined to be species-specific. As a matter of fact, Yersinia enterocolitica, Yersinia intermedia, Yersinia frederiskenii and Yersinia kristensenii showed differential migrations in the gels. Moreover, Y. enterocolitica serotypes O:3, O:5 and O:9 were distinguishable, as well. Only for a limited number of traditionally isolated strains, the biochemical and molecular identification agree. In particular, an overestimation of Y. enterocolitica, as determined biochemically, was observed. Finally when the protocol was applied to 27 food samples, a good correlation was obtained when the results of traditional and direct methods were analyzed. The molecular method was able to identify Y. enterocolitica, not detected by plating analysis. However, for 4 samples, that, by plating analysis, were determined to contain Yersinia spp., no PCR product could be obtained after enrichment, probably due to low numbers of target cells, thereby not allowing the possibility to perform DGGE analysis. The protocol described here represents a reliable tool for the detection of Yersinia spp. in food, which can be used to obtain the needed results faster than with traditional culturing methods.
URL: 16085330
Data: (CIP 80. 30) Type strain / Centre Nationale des Yersinia / Bercovier, H. et al. (1980) Current Microbiology 4, 210-224
Accession Date: 01/01/1982
History: CENTRE NATIONALE DES YERSINIA;CPHL,COLINDALE,LONDON
Authority: Bercovier et al. 1981 (ALI)
Depositor: HOLMES B
Taxonomy: TaxLink: S3614 (Yersinia kristensenii Bercovier et al. 1981) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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