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Bacteria Collection: Vibrio mimicus

NCTC Number: NCTC 11435
Current Name: Vibrio mimicus
Original Strain Reference: CDC 1721 - 77
Other Collection No: ATCC 33653; CCM 4552; CCUG 13624; CDC 1721-77; DSM 19130; LMG 7896
Previous Catalogue Name: Vibrio mimicus
Other Names: VIBRIO METSCHNIKOVII, GAMALEIA 1888
Type Strain: Yes
Family: Vibrionaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, human ear infection
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS950474
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AF114747|RC5| ATCC33653T|Vibrio mimicus RC5 16S-23S ribosomal RNA intergenic spacer regiontype 1.| cacgatgattatcgt... >gb|AF114746|RC5| ATCC33653T|Vibrio mimicus RC5 16S-23S ribosomal RNA intergenic spacer regiontype 3.| cacgatgattattgt... >gb|AF114745|RC5| ATCC33653T|Vibrio mimicus RC5 16S-23S ribosomal RNA intergenic spacer regiontype 3.| cacgatgattatcgt... >gb|AF114744|RC5| ATCC33653T|Vibrio mimicus RC5 16S-23S ribosomal RNA intergenic spacer regiontype 2.| cacgatgattatcgt... >gb|X74713|ATCC 33653T|V.mimicus (ATCC 33653T) gene for 16S ribosomal RNA.| attgaagagtttgat...
23S rRNA Gene Sequence: >gb|AF114747|RC5| ATCC33653T|Vibrio mimicus RC5 16S-23S ribosomal RNA intergenic spacer regiontype 1.| cacgatgattatcgt... >gb|AF114746|RC5| ATCC33653T|Vibrio mimicus RC5 16S-23S ribosomal RNA intergenic spacer regiontype 3.| cacgatgattattgt... >gb|AF114745|RC5| ATCC33653T|Vibrio mimicus RC5 16S-23S ribosomal RNA intergenic spacer regiontype 3.| cacgatgattatcgt... >gb|AF114744|RC5| ATCC33653T|Vibrio mimicus RC5 16S-23S ribosomal RNA intergenic spacer regiontype 2.| cacgatgattatcgt...
Bibliography: DAVIS B R ET AL 1981 J CLIN MICRO 14 631 639
Extended Bibliography: showhide Show bibliography
Ref #: 93196
Author(s): Ruimy,R.;Breittmayer,V.;Elbaze,P.;Lafay,B.;Boussemart,O.;Gauthier,M.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences
Volume: 44
Page(s): 416-26
Year: 1994
Keyword(s): GENBANK/X74674 GENBANK/X74675 GENBANK/X74676 GENBANK/X74677 GENBANK/X74678 GENBANK/X74679 GENBANK/X74680 GENBANK/X74681 GENBANK/X74682 GENBANK/X74683 GENBANK/X74684 GENBANK/X74685 GENBANK/X74686 GENBANK/X74687 GENBANK/X74688 GENBANK/X74689 GENBANK/X74690 GENBANK/X74691 GENBANK/X74692 GENBANK/X74693 GENBANK/X74694 GENBANK/X74695 GENBANK/X74696 GENBANK/X74697 GENBANK/X74698 GENBANK/X74699 GENBANK/X74700 GENBANK/X74701 GENBANK/X74702 GENBANK/X74703 Aeromonas/classification/genetics Photobacterium/classification/genetics *Phylogeny Plesiomonas/classification/genetics RNA, Bacterial/*genetics RNA, Ribosomal/*genetics Sequence Homology, Nucleic Acid Species Specificity Vibrio/classification/genetics Vibrionaceae/*classification/*genetics
Remarks: We sequenced nearly complete small-subunit rRNAs of 54 reference strains belonging to the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas. We then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the Proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony, as well as bootstrap, to assess the robustness of each topology. Our results confirmed that the family Vibrionaceae should include only Photobacterium and Vibrio species (but not Vibrio marinus); that Aeromonas species deserve family rank; and that Plesiomonas shigelloides is linked to the family Enterobacteriaceae. The genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas, together with the family Enterobacteriaceae, the family Pasteurellaceae, and probably the genus Alteromonas, form a robust monophyletic unit within the gamma 3 subgroup of the Proteobacteria.
URL: 7520733
Ref #: 93293
Author(s): Chun,J.;Huq,A.;Colwell,R.R.
Journal: Appl Environ Microbiol
Title: Analysis of 16S-23S rRNA intergenic spacer regions of Vibrio cholerae and Vibrio mimicus
Volume: 65
Page(s): 2202-8
Year: 1999
Keyword(s): GENBANK/AF114721 GENBANK/AF114722 GENBANK/AF114723 GENBANK/AF114724 GENBANK/AF114725 GENBANK/AF114726 GENBANK/AF114727 GENBANK/AF114728 GENBANK/AF114729 GENBANK/AF114730 GENBANK/AF114731 GENBANK/AF114732 GENBANK/AF114733 GENBANK/AF114734 GENBANK/AF114735 GENBANK/AF114736 GENBANK/AF114737 GENBANK/AF114738 GENBANK/AF114739 GENBANK/AF114740 GENBANK/AF114741 GENBANK/AF114742 GENBANK/AF114743 GENBANK/AF114744 GENBANK/AF114745 GENBANK/AF114746 GENBANK/AF114747 GENBANK/AF114748 GENBANK/AF114749 Base Sequence DNA Primers/genetics Genes, Bacterial Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Homology, Nucleic Acid Species Specificity Vibrio/classification/*genetics Vibrio cholerae/classification/*genetics
Remarks: Vibrio cholerae identification based on molecular sequence data has been hampered by a lack of sequence variation from the closely related Vibrio mimicus. The two species share many genes coding for proteins, such as ctxAB, and show almost identical 16S DNA coding for rRNA (rDNA) sequences. Primers targeting conserved sequences flanking the 3' end of the 16S and the 5' end of the 23S rDNAs were used to amplify the 16S-23S rRNA intergenic spacer regions of V. cholerae and V. mimicus. Two major (ca. 580 and 500 bp) and one minor (ca. 750 bp) amplicons were consistently generated for both species, and their sequences were determined. The largest fragment contains three tRNA genes (tDNAs) coding for tRNAGlu, tRNALys, and tRNAVal, which has not previously been found in bacteria examined to date. The 580-bp amplicon contained tDNAIle and tDNAAla, whereas the 500-bp fragment had single tDNA coding either tRNAGlu or tRNAAla. Little variation, i.e., 0 to 0.4%, was found among V. cholerae O1 classical, O1 El Tor, and O139 epidemic strains. Slightly more variation was found against the non-O1/non-O139 serotypes (ca. 1% difference) and V. mimicus (2 to 3% difference). A pair of oligonucleotide primers were designed, based on the region differentiating all of V. cholerae strains from V. mimicus. The PCR system developed was subsequently evaluated by using representatives of V. cholerae from environmental and clinical sources, and of other taxa, including V. mimicus. This study provides the first molecular tool for identifying the species V. cholerae.
URL: 10224020
Ref #: 12044
Author(s): Ruimy,R.;Breittmayer,V.;Elbaze,P.;Lafay,B.;Boussemart,O.;Gauthier,M.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences
Volume: 44
Page(s): 416-26
Year: 1994
Keyword(s): GENBANK/X74674 GENBANK/X74675 GENBANK/X74676 GENBANK/X74677 GENBANK/X74678 GENBANK/X74679 GENBANK/X74680 GENBANK/X74681 GENBANK/X74682 GENBANK/X74683 GENBANK/X74684 GENBANK/X74685 GENBANK/X74686 GENBANK/X74687 GENBANK/X74688 GENBANK/X74689 GENBANK/X74690 GENBANK/X74691 GENBANK/X74692 GENBANK/X74693 GENBANK/X74694 GENBANK/X74695 GENBANK/X74696 GENBANK/X74697 GENBANK/X74698 GENBANK/X74699 GENBANK/X74700 GENBANK/X74701 GENBANK/X74702 GENBANK/X74703 Aeromonas/classification/genetics Comparative Study Photobacterium/classification/genetics *Phylogeny Plesiomonas/classification/genetics RNA, Bacterial/*genetics RNA, Ribosomal/*genetics Sequence Homology, Nucleic Acid Species Specificity Support, Non-U.S. Gov't Vibrio/classification/genetics Vibrionaceae/*classification/*genetics
Remarks: We sequenced nearly complete small-subunit rRNAs of 54 reference strains belonging to the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas. We then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the Proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony, as well as bootstrap, to assess the robustness of each topology. Our results confirmed that the family Vibrionaceae should include only Photobacterium and Vibrio species (but not Vibrio marinus); that Aeromonas species deserve family rank; and that Plesiomonas shigelloides is linked to the family Enterobacteriaceae. The genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas, together with the family Enterobacteriaceae, the family Pasteurellaceae, and probably the genus Alteromonas, form a robust monophyletic unit within the gamma 3 subgroup of the Proteobacteria.
URL: 94347604
Ref #: 13616
Author(s): Chun,J.;Huq,A.;Colwell,R.R.
Journal: Appl Environ Microbiol
Title: Analysis of 16S-23S rRNA intergenic spacer regions of Vibrio cholerae and Vibrio mimicus
Volume: 65
Page(s): 2202-8
Year: 1999
Keyword(s): GENBANK/AF114721 GENBANK/AF114722 GENBANK/AF114723 GENBANK/AF114724 GENBANK/AF114725 GENBANK/AF114726 GENBANK/AF114727 GENBANK/AF114728 GENBANK/AF114729 GENBANK/AF114730 GENBANK/AF114731 GENBANK/AF114732 GENBANK/AF114733 GENBANK/AF114734 GENBANK/AF114735 GENBANK/AF114736 GENBANK/AF114737 GENBANK/AF114738 GENBANK/AF114739 GENBANK/AF114740 GENBANK/AF114741 GENBANK/AF114742 GENBANK/AF114743 GENBANK/AF114744 GENBANK/AF114745 GENBANK/AF114746 GENBANK/AF114747 GENBANK/AF114748 GENBANK/AF114749 Base Sequence Comparative Study DNA Primers/genetics Genes, Bacterial Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Homology, Nucleic Acid Species Specificity Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Vibrio/classification/*genetics Vibrio cholerae/classification/*genetics
Remarks: Vibrio cholerae identification based on molecular sequence data has been hampered by a lack of sequence variation from the closely related Vibrio mimicus. The two species share many genes coding for proteins, such as ctxAB, and show almost identical 16S DNA coding for rRNA (rDNA) sequences. Primers targeting conserved sequences flanking the 3' end of the 16S and the 5' end of the 23S rDNAs were used to amplify the 16S-23S rRNA intergenic spacer regions of V. cholerae and V. mimicus. Two major (ca. 580 and 500 bp) and one minor (ca. 750 bp) amplicons were consistently generated for both species, and their sequences were determined. The largest fragment contains three tRNA genes (tDNAs) coding for tRNAGlu, tRNALys, and tRNAVal, which has not previously been found in bacteria examined to date. The 580-bp amplicon contained tDNAIle and tDNAAla, whereas the 500-bp fragment had single tDNA coding either tRNAGlu or tRNAAla. Little variation, i.e., 0 to 0.4%, was found among V. cholerae O1 classical, O1 El Tor, and O139 epidemic strains. Slightly more variation was found against the non-O1/non-O139 serotypes (ca. 1% difference) and V. mimicus (2 to 3% difference). A pair of oligonucleotide primers were designed, based on the region differentiating all of V. cholerae strains from V. mimicus. The PCR system developed was subsequently evaluated by using representatives of V. cholerae from environmental and clinical sources, and of other taxa, including V. mimicus. This study provides the first molecular tool for identifying the species V. cholerae.
URL: 99240450
Data: (ATCC 33653) Type strain / D. J. Brenner, CDC Atlanta in 1982 / State Board of Health, N. Carolina / Human ear infection / Davis, B. R. et al. (1981) J. clin. Micro. 14, 631-639
Accession Date: 01/01/1982
History: ISOLATED BY STATE BOARD OF HEALTH N CAROLINA 1977
Authority: Davis et al. 1982
Depositor: BRENNER D J
Taxonomy: TaxLink: S3556 (Vibrio mimicus Davis et al. 1982) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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