Culture Collections

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Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Pluralibacter gergoviae

NCTC Number: NCTC 11434
Current Name: Pluralibacter gergoviae
Original Strain Reference: 604 - 77
Other Collection No: ATCC 33028; CDC 604-77; CIP 76.01; DSM 9245; JCM 1234; 6504-77
Previous Catalogue Name: Enterobacter gergoviae
Type Strain: Yes
Family: Enterobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, urinary tract
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS513145
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/d...
16S rRNA Gene Sequence: >gb|AB004748|JCM1234|Enterobacter gergoviae gene for 16S ribosomal RNA, partialsequence.| acgctggcggcaggc...
Miscellaneous Sequence Data: >gb|AY370839|ATCC 33028|Enterobacter gergoviae strain ATCC 33028 gyrase B (gyrB) gene,partial cds.| aaagtctccggcggc...
Bibliography: BRENNER D J ET AL 1980 INT J SYST BACT 30 1 6
Extended Bibliography: showhide Show bibliography
Ref #: 43062
Author(s): Hoffmann,H.;Roggenkamp,A.
Journal: Appl Environ Microbiol
Title: Population genetics of the nomenspecies Enterobacter cloacae
Volume: 69
Page(s): 5306-18
Year: 2003
Keyword(s): Anti-Bacterial Agents/pharmacology Base Sequence Chaperonin 60/genetics DNA Primers DNA, Bacterial/genetics Enterobacter cloacae/*classification/drug effects/*genetics/isolation & purification Humans Microbial Sensitivity Tests Multigene Family Nucleic Acid Hybridization *Phylogeny Polymerase Chain Reaction/methods *Polymorphism, Restriction Fragment Length
Remarks: The genetic heterogeneity of the nomenspecies Enterobacter cloacae is well known. Enterobacter asburiae, Enterobacter cancerogenus, Enterobacter dissolvens, Enterobacter hormaechei, Enterobacter kobei, and Enterobacter nimipressuralis are closely related to it and are subsumed in the so-called E. cloacae complex. DNA-DNA hybridization studies performed previously identified at least five DNA-relatedness groups of this complex. In order to analyze the genetic structure and the phylogenetic relationships between the clusters of the nomenspecies E. cloacae, 206 strains collected from 22 hospitals, a veterinarian, and an agricultural center in 11 countries plus all 13 type strains of the genus and reference strain CDC 1347-71(R) were examined with a combination of sequence and PCR-restriction fragment length polymorphism (PCR-RFLP) analyses of the three housekeeping genes hsp60, rpoB, and hemB as well as ampC, the gene of a class C beta-lactamase. Based on the neighbor-joining tree of the hsp60 sequences, 12 genetic clusters (I to XII) and an unstable sequence crowd (xiii) were identified. The robustness of the genetic clusters was confirmed by analyses of rpoB and hemB sequences and ampC PCR-RFLPs. Sequence crowd xiii split into two groups after rpoB analysis. Only three strains formed a cluster with the type strain of E. cloacae, indicating that the minority of isolates identified as E. cloacae truly belong to the species; 13% of strains grouped with other type strains of the genus, suggesting that the phenotypes of these species seem to be more heterogeneous than so far believed. Three clusters represented 70% of strains, but none of them included a type or reference strain. The genetic clustering presented in this study might serve as a framework for future studies dealing with taxonomic, evolutionary, epidemiological, or pathogenetic characteristics of bacteria belonging to the E. cloacae complex.
URL: 12957918
Ref #: 43181
Author(s): Delmas,J.;Breysse,F.;Devulder,G.;Flandrois,J.P.;Chomarat,M.
Journal: Diagn Microbiol Infect Dis
Title: Rapid identification of Enterobacteriaceae by sequencing DNA gyrase subunit B encoding gene
Volume: 55
Page(s): 263-8
Year: 2006
Keyword(s): Bacterial Typing Techniques/*methods DNA Gyrase/*genetics Enterobacteriaceae/enzymology/genetics/*isolation & purification Genotype Humans Sequence Analysis, DNA/methods
Remarks: Real-time polymerase chain reaction and sequencing were used to characterize a 506-bp-long DNA fragment internal to the gyrB gene (gyrBint). The sequences obtained from 32 Enterobacteriaceae-type strains and those available in the Genbank nucleotide sequence database (n = 24) were used as a database to identify 240 clinical enterobacteria isolates. Sequence analysis of the gyrBint fragment of 240 strains showed that gyrBint constitutes a discriminative target sequence to differentiate between Enterobacteriaceae species. Comparison of these identifications with those obtained by phenotypic methods (Vitek 1 system and/or Rapid ID 32E; bioMerieux, Marcy l'Etoile, France) revealed discrepancies essentially with genera Citrobacter and Enterobacter. Most of the strains identified as Enterobacter cloacae by phenotypic methods were identified as Enterobacter hormaechei strains by gyrBint sequencing. The direct sequencing of gyrBint would be useful as a complementary tool in the identification of clinical Enterobacteriaceae isolates.
URL: 16626902
Ref #: 5171
Author(s): Brenner,D.J.;Richard,C.;Steigerwalt,A.G.;Asbury,M.A.;Mandel,M.
Journal: Int. J. Syst. Bacteriol.
Title: Enterobacter gergoviae sp.nov.: A new species of Enterobacteriaceae found in clinical specimens and the environment.
Volume: 30
Page(s): 1-6
Year: 1980
Data: (ATCC 33028; CIP 76. 01) Type strain / D. J. Brenner, CDC Atlanta in 1982 / C. Richard, Institut Pasteur in 1977 / Urinary tract / Brenner, D. J. et al. (1980) Int. J. syst. Bact. 30, 1-6
Accession Date: 01/01/1982
History: ISOLATED BY RICHARD C INSTITUT PASTEUR PARIS-BRENNER CDC PRE:FR
Authority: Brenner et al. 1980
Depositor: BRENNER D J
Taxonomy: TaxLink: S1135 (Enterobacter gergoviae Brenner et al. 1980) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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