Extended Bibliography: |
Show bibliography
Ref #: |
32603 |
Author(s): |
Linton,D.;Hurtado,A.;Lawson,A.J.;Clewley,J.P.;Chart,H.;Stanley,J. |
Journal: |
Res Microbiol |
Title: |
Campylobacter coli strains with enlarged flagellin genes isolated from river water |
Volume: |
150 |
Page(s): |
247-55 |
Year: |
1999 |
Keyword(s): |
GENBANK/Y11762
GENBANK/Y11763
GENBANK/Y11764
GENBANK/Y11765
GENBANK/Y11766
Amino Acid Sequence
Base Sequence
Campylobacter coli/classification/*genetics
Flagellin/*genetics
Fresh Water/*microbiology
Genotype
Molecular Sequence Data
Phenotype
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S/chemistry
RNA, Ribosomal, 23S/chemistry
RNA, Viral/chemistry
Sequence Alignment
*Water Microbiology
|
Remarks: |
A group of campylobacters isolated from river water were found to possess unusually large flagellin genes. Both phenotype and serology were consistent with identification as Campylobacter coli. Phylogenetic analysis of small (16S, rrs) and large subunit (23S, rrl) rRNA genes of a representative strain, NCTC 13006, demonstrated high levels of relatedness with C. jejuni and C. coli (99.1 and 98.3% similarity for 16S; 99.3 and 99.4% similarity for 23S). Large flagellin proteins were demonstrated by SDS-PAGE analysis. The flaA and flaB genes were sequenced and aligned with known campylobacter flagellin amino acid sequences. The encoded FlaA protein of the new group exhibited a high degree of divergence from other Campylobacter species. Within the central variable region of FlaA, a further hypervariable domain was identified containing characteristic repeated motifs. Separate pairwise alignments performed for the variable regions of the polypeptide indicated these large fla genes were more closely related to those of C. upsaliensis than to those of C. coli or C. jejuni. |
URL: |
10376486 |
|
Ref #: |
95450 |
Author(s): |
Korczak,B.M.;Stieber,R.;Emler,S.;Burnens,A.P.;Frey,J.;Kuhnert,P. |
Journal: |
Int J Syst Evol Microbiol |
Title: |
Genetic relatedness within the genus Campylobacter inferred from rpoB sequences |
Volume: |
56 |
Page(s): |
937-45 |
Year: |
2006 |
Keyword(s): |
Bacterial Proteins/*genetics
Base Sequence
Campylobacter/*classification/*genetics
DNA Primers
DNA, Bacterial/chemistry/genetics
DNA, Ribosomal/chemistry/genetics
DNA-Directed RNA Polymerases/*genetics
Molecular Sequence Data
*Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Statistics
|
Remarks: |
The genus Campylobacter comprises 17 species, some of which are important animal and human pathogens. To gain more insight into the genetic relatedness of this genus and to improve the molecular tools available for diagnosis, a universal sequencing approach was established for the gene encoding the beta-subunit of RNA polymerase (rpoB) for the genus Campylobacter. A total of 59 strains, including the type strains of currently recognized species as well as field isolates, were investigated in the study. A primer set specific for Campylobacter species enabled straightforward amplification and sequencing of a 530 bp fragment of the rpoB gene. The 16S rRNA gene sequences of all of the strains were determined in parallel. A good congruence was obtained between 16S rRNA and rpoB gene sequence-based trees within the genus Campylobacter. The branching of the rpoB tree was similar to that of the 16S rRNA gene tree, even though a few discrepancies were observed for certain species. The resolution of the rpoB gene within the genus Campylobacter was generally much higher than that of the 16S rRNA gene sequence, resulting in a clear separation of most species and even some subspecies. The universally applicable amplification and sequencing approach for partial rpoB gene sequence determination provides a powerful tool for DNA sequence-based discrimination of Campylobacter species. |
URL: |
16627635 |
|
Ref #: |
12361 |
Author(s): |
Linton,D.;Hurtado,A.;Lawson,A.J.;Clewley,J.P.;Chart,H.;Stanley,J. |
Journal: |
Res Microbiol |
Title: |
Campylobacter coli strains with enlarged flagellin genes isolated from river water |
Volume: |
150 |
Page(s): |
247-55 |
Year: |
1999 |
Keyword(s): |
GENBANK/Y11762
GENBANK/Y11763
GENBANK/Y11764
GENBANK/Y11765
GENBANK/Y11766
Amino Acid Sequence
Base Sequence
Campylobacter coli/classification/*genetics
Flagellin/*genetics
Fresh Water/*microbiology
Genotype
Molecular Sequence Data
Phenotype
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S/chemistry
RNA, Ribosomal, 23S/chemistry
RNA, Viral/chemistry
Sequence Alignment
Support, Non-U.S. Gov't
*Water Microbiology
|
Remarks: |
A group of campylobacters isolated from river water were found to possess unusually large flagellin genes. Both phenotype and serology were consistent with identification as Campylobacter coli. Phylogenetic analysis of small (16S, rrs) and large subunit (23S, rrl) rRNA genes of a representative strain, NCTC 13006, demonstrated high levels of relatedness with C. jejuni and C. coli (99.1 and 98.3% similarity for 16S; 99.3 and 99.4% similarity for 23S). Large flagellin proteins were demonstrated by SDS-PAGE analysis. The flaA and flaB genes were sequenced and aligned with known campylobacter flagellin amino acid sequences. The encoded FlaA protein of the new group exhibited a high degree of divergence from other Campylobacter species. Within the central variable region of FlaA, a further hypervariable domain was identified containing characteristic repeated motifs. Separate pairwise alignments performed for the variable regions of the polypeptide indicated these large fla genes were more closely related to those of C. upsaliensis than to those of C. coli or C. jejuni. |
URL: |
99302979 |
|
Ref #: |
2728 |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 14. |
Volume: |
34 |
Page(s): |
270-271 |
Year: |
1984 |
|
Ref #: |
5530 |
Author(s): |
Benjamin,J.;Leaper,S.;Owen,R.J.;Skirrow,M.B. |
Journal: |
Curr. Microbiol. |
Title: |
Description of Campylobacter laridis, a new species comprising the nalidixic acid resistant thermophilic Campylobacter (NARTC) group. |
Volume: |
8 |
Page(s): |
231-238 |
Year: |
1983 |
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