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Bacteria Collection: Campylobacter lari

NCTC Number: NCTC 11352
Current Name: Campylobacter lari
Original Strain Reference: 3034/77
Other Collection No: ATCC 35221; CCUG 10773; DSM 11375; LMG 7605; NARTC GROUP; WRI 3034/77; WDCM 00204
Previous Catalogue Name: Campylobacter lari
Type Strain: Yes
Family: Campylobacteraceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48 hours, 37°C, microaerophilic
Conditions for growth on liquid media: nutrient broth,37, microaerophilic
Isolated From: bird;cloacal swab of herring gull
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS950468
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|DQ174145|NCTC 11352|Campylobacter lari strain NCTC 11352 16S ribosomal RNA (rrs) gene,partial sequence.| agtgaacgctggcgg... >gb|AY621114|ATCC 35221|Campylobacter lari strain ATCC 35221 16S ribosomal RNA gene,partial sequence.| gtgcctaatacatgc...
23S rRNA Gene Sequence: >gb|Y11764|NCTC 11352|C.lari 23S rRNA gene.| gatttccgaatgggg...
Bibliography: BENJAMIN J ET AL 1983 CURR MICROBIOL
Extended Bibliography: showhide Show bibliography
Ref #: 32603
Author(s): Linton,D.;Hurtado,A.;Lawson,A.J.;Clewley,J.P.;Chart,H.;Stanley,J.
Journal: Res Microbiol
Title: Campylobacter coli strains with enlarged flagellin genes isolated from river water
Volume: 150
Page(s): 247-55
Year: 1999
Keyword(s): GENBANK/Y11762 GENBANK/Y11763 GENBANK/Y11764 GENBANK/Y11765 GENBANK/Y11766 Amino Acid Sequence Base Sequence Campylobacter coli/classification/*genetics Flagellin/*genetics Fresh Water/*microbiology Genotype Molecular Sequence Data Phenotype Phylogeny Polymerase Chain Reaction Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S/chemistry RNA, Ribosomal, 23S/chemistry RNA, Viral/chemistry Sequence Alignment *Water Microbiology
Remarks: A group of campylobacters isolated from river water were found to possess unusually large flagellin genes. Both phenotype and serology were consistent with identification as Campylobacter coli. Phylogenetic analysis of small (16S, rrs) and large subunit (23S, rrl) rRNA genes of a representative strain, NCTC 13006, demonstrated high levels of relatedness with C. jejuni and C. coli (99.1 and 98.3% similarity for 16S; 99.3 and 99.4% similarity for 23S). Large flagellin proteins were demonstrated by SDS-PAGE analysis. The flaA and flaB genes were sequenced and aligned with known campylobacter flagellin amino acid sequences. The encoded FlaA protein of the new group exhibited a high degree of divergence from other Campylobacter species. Within the central variable region of FlaA, a further hypervariable domain was identified containing characteristic repeated motifs. Separate pairwise alignments performed for the variable regions of the polypeptide indicated these large fla genes were more closely related to those of C. upsaliensis than to those of C. coli or C. jejuni.
URL: 10376486
Ref #: 95450
Author(s): Korczak,B.M.;Stieber,R.;Emler,S.;Burnens,A.P.;Frey,J.;Kuhnert,P.
Journal: Int J Syst Evol Microbiol
Title: Genetic relatedness within the genus Campylobacter inferred from rpoB sequences
Volume: 56
Page(s): 937-45
Year: 2006
Keyword(s): Bacterial Proteins/*genetics Base Sequence Campylobacter/*classification/*genetics DNA Primers DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics DNA-Directed RNA Polymerases/*genetics Molecular Sequence Data *Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Statistics
Remarks: The genus Campylobacter comprises 17 species, some of which are important animal and human pathogens. To gain more insight into the genetic relatedness of this genus and to improve the molecular tools available for diagnosis, a universal sequencing approach was established for the gene encoding the beta-subunit of RNA polymerase (rpoB) for the genus Campylobacter. A total of 59 strains, including the type strains of currently recognized species as well as field isolates, were investigated in the study. A primer set specific for Campylobacter species enabled straightforward amplification and sequencing of a 530 bp fragment of the rpoB gene. The 16S rRNA gene sequences of all of the strains were determined in parallel. A good congruence was obtained between 16S rRNA and rpoB gene sequence-based trees within the genus Campylobacter. The branching of the rpoB tree was similar to that of the 16S rRNA gene tree, even though a few discrepancies were observed for certain species. The resolution of the rpoB gene within the genus Campylobacter was generally much higher than that of the 16S rRNA gene sequence, resulting in a clear separation of most species and even some subspecies. The universally applicable amplification and sequencing approach for partial rpoB gene sequence determination provides a powerful tool for DNA sequence-based discrimination of Campylobacter species.
URL: 16627635
Ref #: 12361
Author(s): Linton,D.;Hurtado,A.;Lawson,A.J.;Clewley,J.P.;Chart,H.;Stanley,J.
Journal: Res Microbiol
Title: Campylobacter coli strains with enlarged flagellin genes isolated from river water
Volume: 150
Page(s): 247-55
Year: 1999
Keyword(s): GENBANK/Y11762 GENBANK/Y11763 GENBANK/Y11764 GENBANK/Y11765 GENBANK/Y11766 Amino Acid Sequence Base Sequence Campylobacter coli/classification/*genetics Flagellin/*genetics Fresh Water/*microbiology Genotype Molecular Sequence Data Phenotype Phylogeny Polymerase Chain Reaction Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S/chemistry RNA, Ribosomal, 23S/chemistry RNA, Viral/chemistry Sequence Alignment Support, Non-U.S. Gov't *Water Microbiology
Remarks: A group of campylobacters isolated from river water were found to possess unusually large flagellin genes. Both phenotype and serology were consistent with identification as Campylobacter coli. Phylogenetic analysis of small (16S, rrs) and large subunit (23S, rrl) rRNA genes of a representative strain, NCTC 13006, demonstrated high levels of relatedness with C. jejuni and C. coli (99.1 and 98.3% similarity for 16S; 99.3 and 99.4% similarity for 23S). Large flagellin proteins were demonstrated by SDS-PAGE analysis. The flaA and flaB genes were sequenced and aligned with known campylobacter flagellin amino acid sequences. The encoded FlaA protein of the new group exhibited a high degree of divergence from other Campylobacter species. Within the central variable region of FlaA, a further hypervariable domain was identified containing characteristic repeated motifs. Separate pairwise alignments performed for the variable regions of the polypeptide indicated these large fla genes were more closely related to those of C. upsaliensis than to those of C. coli or C. jejuni.
URL: 99302979
Ref #: 2728
Journal: Int. J. Syst. Bacteriol.
Title: Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 14.
Volume: 34
Page(s): 270-271
Year: 1984
Ref #: 5530
Author(s): Benjamin,J.;Leaper,S.;Owen,R.J.;Skirrow,M.B.
Journal: Curr. Microbiol.
Title: Description of Campylobacter laridis, a new species comprising the nalidixic acid resistant thermophilic Campylobacter (NARTC) group.
Volume: 8
Page(s): 231-238
Year: 1983
Data: (ATCC 35221, CCUG 10773, LMG 7605) Type strain / M. B. Skirrow in 1980 / Cloacal swab of herring gull (Larus argentatus) / Benjamin, J. et al. (1983) Curr. Microbiol. 8, 231
Accession Date: 01/01/1980
History: MICROBIOLOGY DEPT,WORCESTER ROYAL INFIRMARY
Authority: Benjamin et al. 1984
Depositor: SKIRROW M B
Taxonomy: TaxLink: S676 (Campylobacter lari benjamin et al. 1984) - Date of change: 16/06/2007 by NCTCUp to 16/06/2007: ? (NCTC 11352) - Date of change: 04/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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