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Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Vibrio natriegens

NCTC Number: NCTC 11319
Current Name: Vibrio natriegens
Original Strain Reference: 111 (P. Baumann)
Other Collection No: ATCC 14048; CAIM 12; CCUG 16371; CCUG 4980 A; CIP 103193; DSM 759; LMG 10935; NBRC 15636; NCCB 73019; NCMB 857
Previous Catalogue Name: Beneckea natriegens
Type Strain: Yes
Family: Vibrionaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic, requires carbon dioxide
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: salt water/mud;salt marsh mud
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1247846
16S rRNA Gene Sequence: >gb|X56581 S83488 S83495|ATCC 14048|V.natrigens 16S ribosomal RNA.| naaattgaagagttt... >gb|X74714|ATCC 14048T|V.natriegens (ATCC 14048T) gene for 16S ribosomal RNA.| gagtttgatcatggc... >gb|DQ068938|ATCC 14048|Vibrio natriegens 16S ribosomal RNA gene, partial sequence.| gctggcggcggccta... >gb|X56581 AH000280 S83488 S83495 S83499|ATCC 14048|V.natrigens 16S ribosomal RNA.| naaattgaagagttt...
Miscellaneous Sequence Data: >gb|AF007291|ATCC 14048|Vibrio natriegens gyrase B subunit (gyrB) gene, partial cds.| tgcnggnggnaaatc...
Bibliography: BAUMANN P ET AL 1971 J BACT 107 291 295
Extended Bibliography: showhide Show bibliography
Ref #: 93196
Author(s): Ruimy,R.;Breittmayer,V.;Elbaze,P.;Lafay,B.;Boussemart,O.;Gauthier,M.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences
Volume: 44
Page(s): 416-26
Year: 1994
Keyword(s): GENBANK/X74674 GENBANK/X74675 GENBANK/X74676 GENBANK/X74677 GENBANK/X74678 GENBANK/X74679 GENBANK/X74680 GENBANK/X74681 GENBANK/X74682 GENBANK/X74683 GENBANK/X74684 GENBANK/X74685 GENBANK/X74686 GENBANK/X74687 GENBANK/X74688 GENBANK/X74689 GENBANK/X74690 GENBANK/X74691 GENBANK/X74692 GENBANK/X74693 GENBANK/X74694 GENBANK/X74695 GENBANK/X74696 GENBANK/X74697 GENBANK/X74698 GENBANK/X74699 GENBANK/X74700 GENBANK/X74701 GENBANK/X74702 GENBANK/X74703 Aeromonas/classification/genetics Photobacterium/classification/genetics *Phylogeny Plesiomonas/classification/genetics RNA, Bacterial/*genetics RNA, Ribosomal/*genetics Sequence Homology, Nucleic Acid Species Specificity Vibrio/classification/genetics Vibrionaceae/*classification/*genetics
Remarks: We sequenced nearly complete small-subunit rRNAs of 54 reference strains belonging to the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas. We then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the Proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony, as well as bootstrap, to assess the robustness of each topology. Our results confirmed that the family Vibrionaceae should include only Photobacterium and Vibrio species (but not Vibrio marinus); that Aeromonas species deserve family rank; and that Plesiomonas shigelloides is linked to the family Enterobacteriaceae. The genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas, together with the family Enterobacteriaceae, the family Pasteurellaceae, and probably the genus Alteromonas, form a robust monophyletic unit within the gamma 3 subgroup of the Proteobacteria.
URL: 7520733
Ref #: 95519
Author(s): Venkateswaran,K.;Dohmoto,N.;Harayama,S.
Journal: Appl Environ Microbiol
Title: Cloning and nucleotide sequence of the gyrB gene of Vibrio parahaemolyticus and its application in detection of this pathogen in shrimp
Volume: 64
Page(s): 681-7
Year: 1998
Keyword(s): GENBANK/AF007287 GENBANK/AF007288 GENBANK/AF007289 GENBANK/AF007290 GENBANK/AF007291 GENBANK/AF007292 GENBANK/AF007293 Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Gyrase DNA Topoisomerases, Type II/*genetics Decapoda (Crustacea)/*microbiology Molecular Sequence Data Polymerase Chain Reaction Sensitivity and Specificity Vibrio parahaemolyticus/*genetics/*isolation & purification
Remarks: Because biochemical testing and 16S rRNA sequence analysis have proven inadequate for the differentiation of Vibrio parahaemolyticus from closely related species, we employed the gyrase B gene (gyrB) as a molecular diagnostic probe. The gyrB genes of V. parahaemolyticus and closely related Vibrio alginolyticus were cloned and sequenced. Oligonucleotide PCR primers were designed for the amplification of a 285-bp fragment from within gyrB specific for V. parahaemolyticus. These primers recognized 117 of 117 reference and wild-type V. parahaemolyticus strains, whereas amplification did not occur when 90 strains of 37 other Vibrio species or 60 strains representing 34 different nonvibrio species were tested. In 100-microliter PCR mixtures, the lower detection limits were 5 CFU for live cells and 4 pg for purified DNA. The possible application of gyrB primers for the routine identification of V. parahaemolyticus in food was examined. We developed and tested a procedure for the specific detection of the target organism in shrimp consisting of an 18-h preenrichment followed by PCR amplification of the 285-bp V. parahaemolyticus-specific fragment. This method enabled us to detect an initial inoculum of 1.5 CFU of V. parahaemolyticus cells per g of shrimp homogenate. By this approach, we were able to detect V. parahaemolyticus in all of 27 shrimp samples artificially inoculated with this bacterium. We present here a rapid, reliable, and sensitive protocol for the detection of V. parahaemolyticus in shrimp.
URL: 9464408
Ref #: 27372
Author(s): Dorsch,M.;Lane,D.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences
Volume: 42
Page(s): 58-63
Year: 1992
Keyword(s): GENBANK/L05178 GENBANK/X54744 GENBANK/X54745 GENBANK/X56575 GENBANK/X56576 GENBANK/X56577 GENBANK/X56578 GENBANK/X56579 GENBANK/X56580 GENBANK/X56581 GENBANK/X56582 GENBANK/X56583 Base Sequence Molecular Sequence Data Phylogeny RNA Probes RNA, Bacterial/*chemistry RNA, Ribosomal, 16S/*chemistry Vibrio/*classification/genetics/isolation & purification
Remarks: The inter- and intrageneric relationships of the genus Vibrio were investigated by performing a comparative analysis of the 16S rRNAs of 10 species, including four pathogenic representatives. The results of immunological and 5S rRNA studies were confirmed in that the genus is a neighboring taxon of the family Enterobacteriaceae. With regard to the intrageneric structure, Vibrio alginolyticus, Vibrio campbellii, Vibrio natriegens, Vibrio harveyi, Vibrio proteolyticus, Vibrio parahaemolyticus, and Vibrio vulnificus form the core of the genus, while Vibrio (Listonella) anguillarum, Vibrio diazotrophicus, and Vibrio hollisae are placed on the outskirts of the genus. Variable regions around positions 80, 180, and 450 could be used as target sites for genus- and species-specific oligonucleotide probes and polymerase chain reaction primers to be used in molecular identification.
URL: 1371064
Ref #: 95445
Author(s): Eiler,A.;Bertilsson,S.
Journal: J Microbiol Methods
Title: Detection and quantification of Vibrio populations using denaturant gradient gel electrophoresis
Volume: 67
Page(s): 339-48
Year: 2006
Keyword(s): DNA, Bacterial/chemistry/genetics Electrophoresis, Polyacrylamide Gel/*methods Formamides/chemistry Oceans and Seas Organic Chemicals/chemistry Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/chemistry/genetics Sequence Analysis, DNA Sweden Urea/chemistry Vibrio/genetics/*isolation & purification *Water Microbiology
Remarks: Bacteria affiliated with the genus Vibrio are endemic in marine and estuarine ecosystems and are also found in many freshwater environments. Vibrios can enter viable but non-culturable states and since many species are pathogenic, there is a great need for culture-independent methods that identify and quantify multiple Vibrio populations. We adopted Vibrio-specific 16S rRNA-directed primers and a competitive PCR protocol (QC-PCR; [Thompson, J.R., Randa, M.A., Marcelino, L.A., Tomita-Mitchell, A., Lim, E., Polz, M.F., 2004b. Diversity and dynamics of a North Atlantic coastal Vibrio community. Appl. Environ. Microbiol. 70, 4103-4110]) for separation and quantification of Vibrio populations using denaturant gradient gel electrophoresis (DGGE). Sixteen Vibrio isolates and eight environmental samples were used to assess the precision and resolution of the method. A 45-70% gradient of Urea and formamide enabled separation of Vibrio populations with single nucleotide differences in the amplified fragment. A titration curve for the QC-PCR-DGGE, verified by amending surface water bacterioplankton samples with up to 3 x 10(5)Vibrio cholerae cells, could be approximated by a linear regression of log-transformed values (R(2)=0.96). The limit of detection for single populations was 180 cells per extracted sample or about 4 cells per PCR reaction. Environmental samples from the southern Stockholm archipelago in the Baltic Sea and the more saline coastal waters of Skagerrak each carried between 2 and 6 Vibrio populations, and there were major differences between the locations. Notably, multiple Vibrio populations could be detected and quantified against a background of native bacterioplankton exceeding Vibrio population abundance by more than 6 orders of magnitude. Putative identification based on migration in the DGGE gel was verified by parallel cloning and sequencing of PCR products, and representative clones were also characterized by DGGE. This general approach could also be useful for targeting other phylogenetically constrained bacterial groups and assess their abundance and distribution in complex environmental settings.
URL: 16730823
Ref #: 12044
Author(s): Ruimy,R.;Breittmayer,V.;Elbaze,P.;Lafay,B.;Boussemart,O.;Gauthier,M.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences
Volume: 44
Page(s): 416-26
Year: 1994
Keyword(s): GENBANK/X74674 GENBANK/X74675 GENBANK/X74676 GENBANK/X74677 GENBANK/X74678 GENBANK/X74679 GENBANK/X74680 GENBANK/X74681 GENBANK/X74682 GENBANK/X74683 GENBANK/X74684 GENBANK/X74685 GENBANK/X74686 GENBANK/X74687 GENBANK/X74688 GENBANK/X74689 GENBANK/X74690 GENBANK/X74691 GENBANK/X74692 GENBANK/X74693 GENBANK/X74694 GENBANK/X74695 GENBANK/X74696 GENBANK/X74697 GENBANK/X74698 GENBANK/X74699 GENBANK/X74700 GENBANK/X74701 GENBANK/X74702 GENBANK/X74703 Aeromonas/classification/genetics Comparative Study Photobacterium/classification/genetics *Phylogeny Plesiomonas/classification/genetics RNA, Bacterial/*genetics RNA, Ribosomal/*genetics Sequence Homology, Nucleic Acid Species Specificity Support, Non-U.S. Gov't Vibrio/classification/genetics Vibrionaceae/*classification/*genetics
Remarks: We sequenced nearly complete small-subunit rRNAs of 54 reference strains belonging to the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas. We then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the Proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony, as well as bootstrap, to assess the robustness of each topology. Our results confirmed that the family Vibrionaceae should include only Photobacterium and Vibrio species (but not Vibrio marinus); that Aeromonas species deserve family rank; and that Plesiomonas shigelloides is linked to the family Enterobacteriaceae. The genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas, together with the family Enterobacteriaceae, the family Pasteurellaceae, and probably the genus Alteromonas, form a robust monophyletic unit within the gamma 3 subgroup of the Proteobacteria.
URL: 94347604
Ref #: 13615
Author(s): Dorsch,M.;Lane,D.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences
Volume: 42
Page(s): 58-63
Year: 1992
Keyword(s): GENBANK/L05178 GENBANK/X54744 GENBANK/X54745 GENBANK/X56575 GENBANK/X56576 GENBANK/X56577 GENBANK/X56578 GENBANK/X56579 GENBANK/X56580 GENBANK/X56581 GENBANK/X56582 GENBANK/X56583 Base Sequence Molecular Sequence Data Phylogeny RNA Probes RNA, Bacterial/*chemistry RNA, Ribosomal, 16S/*chemistry Support, Non-U.S. Gov't Vibrio/*classification/genetics/isolation & purification
Remarks: The inter- and intrageneric relationships of the genus Vibrio were investigated by performing a comparative analysis of the 16S rRNAs of 10 species, including four pathogenic representatives. The results of immunological and 5S rRNA studies were confirmed in that the genus is a neighboring taxon of the family Enterobacteriaceae. With regard to the intrageneric structure, Vibrio alginolyticus, Vibrio campbellii, Vibrio natriegens, Vibrio harveyi, Vibrio proteolyticus, Vibrio parahaemolyticus, and Vibrio vulnificus form the core of the genus, while Vibrio (Listonella) anguillarum, Vibrio diazotrophicus, and Vibrio hollisae are placed on the outskirts of the genus. Variable regions around positions 80, 180, and 450 could be used as target sites for genus- and species-specific oligonucleotide probes and polymerase chain reaction primers to be used in molecular identification.
URL: 92144377
Data: (ATCC 14018) ATCC in 1979 / W. J. Payne, University of Georgia / Salt marsh mud / Baumann, P. et al. (1971) J. Bact. 107, 291 and 295
Accession Date: 01/01/1979
History: ATCC;PAYNE W J,UNIVERSITY OF GEORGIA
Authority: (Payne et al. 1961) Baumann et al. 1981
Depositor: GHERNA R L
Taxonomy: TaxLink: S3557 (Beneckea natriegens) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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