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Bacteria and Mycoplasmas detail

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Bacteria Collection: Vibrio campbellii

NCTC Number: NCTC 11317
Current Name: Vibrio campbellii
Original Strain Reference: ATCC 25920
Other Collection No: ATCC 25920; CCM 2582; CCUG 4979; CECT 523; CIP 75.10; DSM 19270; LMG 11216; NBRC 15631; NCIMB 1894
Previous Catalogue Name: Beneckea campbellii
Type Strain: Yes
Family: Vibrionaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic, requires carbon dioxide
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: salt water/mud
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1247845
16S rRNA Gene Sequence: >gb|AF412996|ATCC 25920|Vibrio campbellii strain ATCC 25920 16S-23S ribosomal RNAintergenic spacer, partial sequence; tRNA-Ile and tRNA-Ala genes,complete sequence.| ccttatacgatgatt... >gb|AF426824|ATCC 25920|Vibrio campbellii ATCC 25920 16S ribosomal RNA gene, partialsequence.| ggggggtagaatttc... >gb|X56575 S83253 S83256|ATCC 25920|V.campbellii 16S ribosomal RNA.| naaattgaagagttt... >gb|X74692|ATCC 25920T|V.campbelli (ATCC 25920T) gene for 16S ribosomal RNA.| gagtttgatcatggc... >gb|X56575 AH000273 S83253 S83256 S83258|ATCC 25920|V.campbellii 16S ribosomal RNA.| naaattgaagagttt... >gb|AF412997|ATCC 25920|Vibrio campbellii strain ATCC 25920 16S-23S ribosomal RNAintergenic spacer, partial sequence.| ccttatacgatgatt... >gb|AF412990|ATCC 25920|Vibrio campbellii strain ATCC 25920 16S-23S ribosomal RNAintergenic spacer, partial sequence.| ccttatacgatgatt... >gb|D11193|ATCC 25920|Vibrio campbellii 16S rRNA.| ctkgtctgagaggat... >gb|D11242|ATCC 25920|Vibrio campbellii 16S rRNA.| ggtkaaatgcgtaga...
23S rRNA Gene Sequence: >gb|AF412996|ATCC 25920|Vibrio campbellii strain ATCC 25920 16S-23S ribosomal RNAintergenic spacer, partial sequence; tRNA-Ile and tRNA-Ala genes,complete sequence.| ccttatacgatgatt... >gb|AF412997|ATCC 25920|Vibrio campbellii strain ATCC 25920 16S-23S ribosomal RNAintergenic spacer, partial sequence.| ccttatacgatgatt... >gb|AF412990|ATCC 25920|Vibrio campbellii strain ATCC 25920 16S-23S ribosomal RNAintergenic spacer, partial sequence.| ccttatacgatgatt...
Bibliography: BAUMANN P ET AL 1971 J BACT 107 289
Extended Bibliography: showhide Show bibliography
Ref #: 93196
Author(s): Ruimy,R.;Breittmayer,V.;Elbaze,P.;Lafay,B.;Boussemart,O.;Gauthier,M.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences
Volume: 44
Page(s): 416-26
Year: 1994
Keyword(s): GENBANK/X74674 GENBANK/X74675 GENBANK/X74676 GENBANK/X74677 GENBANK/X74678 GENBANK/X74679 GENBANK/X74680 GENBANK/X74681 GENBANK/X74682 GENBANK/X74683 GENBANK/X74684 GENBANK/X74685 GENBANK/X74686 GENBANK/X74687 GENBANK/X74688 GENBANK/X74689 GENBANK/X74690 GENBANK/X74691 GENBANK/X74692 GENBANK/X74693 GENBANK/X74694 GENBANK/X74695 GENBANK/X74696 GENBANK/X74697 GENBANK/X74698 GENBANK/X74699 GENBANK/X74700 GENBANK/X74701 GENBANK/X74702 GENBANK/X74703 Aeromonas/classification/genetics Photobacterium/classification/genetics *Phylogeny Plesiomonas/classification/genetics RNA, Bacterial/*genetics RNA, Ribosomal/*genetics Sequence Homology, Nucleic Acid Species Specificity Vibrio/classification/genetics Vibrionaceae/*classification/*genetics
Remarks: We sequenced nearly complete small-subunit rRNAs of 54 reference strains belonging to the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas. We then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the Proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony, as well as bootstrap, to assess the robustness of each topology. Our results confirmed that the family Vibrionaceae should include only Photobacterium and Vibrio species (but not Vibrio marinus); that Aeromonas species deserve family rank; and that Plesiomonas shigelloides is linked to the family Enterobacteriaceae. The genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas, together with the family Enterobacteriaceae, the family Pasteurellaceae, and probably the genus Alteromonas, form a robust monophyletic unit within the gamma 3 subgroup of the Proteobacteria.
URL: 7520733
Ref #: 72321
Author(s): Kita-Tsukamoto,K.;Oyaizu,H.;Nanba,K.;Simidu,U.
Journal: Int J Syst Bacteriol
Title: Phylogenetic relationships of marine bacteria, mainly members of the family Vibrionaceae, determined on the basis of 16S rRNA sequences
Volume: 43
Page(s): 8-19
Year: 1993
Keyword(s): GENBANK/D11169 GENBANK/D11170 GENBANK/D11171 GENBANK/D11172 GENBANK/D11173 GENBANK/D11174 GENBANK/D11175 GENBANK/D11176 GENBANK/D11177 GENBANK/D11178 GENBANK/D11179 GENBANK/D11180 GENBANK/D11181 GENBANK/D11182 GENBANK/D11183 GENBANK/D11184 GENBANK/D11185 GENBANK/D11186 GENBANK/D11187 GENBANK/D11188 GENBANK/D11189 GENBANK/D11190 GENBANK/D11191 GENBANK/D11192 GENBANK/D11193 GENBANK/D11194 GENBANK/D11195 GENBANK/D11196 GENBANK/D11197 GENBANK/D11198 etc. Base Sequence Molecular Sequence Data *Phylogeny RNA, Ribosomal, 16S/classification/*genetics/*isolation & purification Seawater Sequence Homology, Nucleic Acid Vibrionaceae/*classification/*genetics/metabolism *Water Microbiology
Remarks: The phylogenetic relationships of 50 reference strains, mostly marine bacteria which require Na+ for growth, were determined on the basis of 600 16S rRNA nucleotides by using reverse transcriptase sequencing. Strains belonging to 10 genera were included (four genera of the family Vibrionaceae, the genus Aeromonas of the family Aeromonadaceae, and the genera Alteromonas, Marinomonas, Shewanella, Pseudomonas, and Deleya). The sequences were aligned, the similarity values and evolutionary distance values were determined, and a phylogenetic tree was constructed by using the neighbor-joining method. On the basis of our results, the family Vibrionaceae was separated into at least seven groups (genera and families). Vibrio marinus clearly was on a line of descent that was remote from other vibrios. As determined by the similarity and evolutionary distance values, V. marinus is more distantly related to the family Vibrionaceae than the members of the Aeromonadaceae are. Also, Vibrio cholerae strains formed a separate group with Vibrio mimicus at the genus level. Of 30 species of the Vibrionaceae, 17 formed a large phylogenetic cluster. The genus Listonella was found to be a heterogeneous group, and the species were distributed in various subgroups of the Vibrionaceae. The separation of the family Aeromonadaceae from the family Vibrionaceae and the separation of the genera Marinomonas and Shewanella from the genus Alteromonas were confirmed in this phylogenetic study. However, a marine Pseudomonas species, Pseudomonas nautica, was clearly separated from two terrestrial Pseudomonas species. Each group that was separated by the phylogenetic analysis had characteristic 16S rRNA sequence patterns that were common only to species in that group. Therefore, the characteristic sequences described in this paper may be useful for identification purposes.
URL: 8427811
Ref #: 27519
Author(s): Lee,S.K.;Wang,H.Z.;Law,S.H.;Wu,R.S.;Kong,R.Y.
Journal: Mar Pollut Bull
Title: Analysis of the 16S-23S rDNA intergenic spacers (IGSs) of marine vibrios for species-specific signature DNA sequences
Volume: 44
Page(s): 412-20
Year: 2002
Keyword(s): Base Sequence DNA Primers DNA, Viral/*analysis Molecular Sequence Data Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sensitivity and Specificity Sequence Analysis, DNA Vibrio/*genetics/isolation & purification *Water Microbiology
Remarks: Vibrios are widespread in the marine environment and a few pathogenic species are known to be commonly associated with outbreaks of diarrheal diseases in humans due to the consumption of raw or improperly cooked seafood. However, there are also many Vibrio species which are potentially pathogenic to vertebrate and invertebrate aquatic animals, and of which little is known. In an attempt to develop rapid PCR detection methods for these latter class of vibrios, we have examined the 16S-23S intergenic spacers (IGSs) of 10 lesser-known Vibrio species and successfully developed species-specific primers for eight of them--Vibrio costicola, V. diazotrophicus, V. fluvialis, V. nigripulchritudo, V. proteolyticus, V. salmonicida, V. splendidus and V. tubiashii. The IGS amplicons were amplified using primers complementary to conserved regions of the 16S and 23S rRNA genes, and cloned into plasmid vectors and sequenced. Analysis of the IGS sequences showed that 37 ribosomal RNA (rrn) operons representing seven different IGS types have been cloned from the 10 vibrios. The three IGS types--IGS(0), IGS(IA) and IGS(Glu)--were the most prevalent forms detected. Multiple alignment of representative sequences of these three IGS types from different Vibrio species revealed several domains of high sequence variability, which were used to design species-specific primers for PCR. The specificity of the primers were evaluated using total DNA prepared from different Vibrio species and bacterial genera. The results showed that the PCR method can be used to reliably detect eight of the 10 Vibrio species in marine waters in this study.
URL: 12146824
Ref #: 27372
Author(s): Dorsch,M.;Lane,D.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences
Volume: 42
Page(s): 58-63
Year: 1992
Keyword(s): GENBANK/L05178 GENBANK/X54744 GENBANK/X54745 GENBANK/X56575 GENBANK/X56576 GENBANK/X56577 GENBANK/X56578 GENBANK/X56579 GENBANK/X56580 GENBANK/X56581 GENBANK/X56582 GENBANK/X56583 Base Sequence Molecular Sequence Data Phylogeny RNA Probes RNA, Bacterial/*chemistry RNA, Ribosomal, 16S/*chemistry Vibrio/*classification/genetics/isolation & purification
Remarks: The inter- and intrageneric relationships of the genus Vibrio were investigated by performing a comparative analysis of the 16S rRNAs of 10 species, including four pathogenic representatives. The results of immunological and 5S rRNA studies were confirmed in that the genus is a neighboring taxon of the family Enterobacteriaceae. With regard to the intrageneric structure, Vibrio alginolyticus, Vibrio campbellii, Vibrio natriegens, Vibrio harveyi, Vibrio proteolyticus, Vibrio parahaemolyticus, and Vibrio vulnificus form the core of the genus, while Vibrio (Listonella) anguillarum, Vibrio diazotrophicus, and Vibrio hollisae are placed on the outskirts of the genus. Variable regions around positions 80, 180, and 450 could be used as target sites for genus- and species-specific oligonucleotide probes and polymerase chain reaction primers to be used in molecular identification.
URL: 1371064
Ref #: 12044
Author(s): Ruimy,R.;Breittmayer,V.;Elbaze,P.;Lafay,B.;Boussemart,O.;Gauthier,M.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences
Volume: 44
Page(s): 416-26
Year: 1994
Keyword(s): GENBANK/X74674 GENBANK/X74675 GENBANK/X74676 GENBANK/X74677 GENBANK/X74678 GENBANK/X74679 GENBANK/X74680 GENBANK/X74681 GENBANK/X74682 GENBANK/X74683 GENBANK/X74684 GENBANK/X74685 GENBANK/X74686 GENBANK/X74687 GENBANK/X74688 GENBANK/X74689 GENBANK/X74690 GENBANK/X74691 GENBANK/X74692 GENBANK/X74693 GENBANK/X74694 GENBANK/X74695 GENBANK/X74696 GENBANK/X74697 GENBANK/X74698 GENBANK/X74699 GENBANK/X74700 GENBANK/X74701 GENBANK/X74702 GENBANK/X74703 Aeromonas/classification/genetics Comparative Study Photobacterium/classification/genetics *Phylogeny Plesiomonas/classification/genetics RNA, Bacterial/*genetics RNA, Ribosomal/*genetics Sequence Homology, Nucleic Acid Species Specificity Support, Non-U.S. Gov't Vibrio/classification/genetics Vibrionaceae/*classification/*genetics
Remarks: We sequenced nearly complete small-subunit rRNAs of 54 reference strains belonging to the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas. We then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the Proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony, as well as bootstrap, to assess the robustness of each topology. Our results confirmed that the family Vibrionaceae should include only Photobacterium and Vibrio species (but not Vibrio marinus); that Aeromonas species deserve family rank; and that Plesiomonas shigelloides is linked to the family Enterobacteriaceae. The genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas, together with the family Enterobacteriaceae, the family Pasteurellaceae, and probably the genus Alteromonas, form a robust monophyletic unit within the gamma 3 subgroup of the Proteobacteria.
URL: 94347604
Ref #: 13615
Author(s): Dorsch,M.;Lane,D.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences
Volume: 42
Page(s): 58-63
Year: 1992
Keyword(s): GENBANK/L05178 GENBANK/X54744 GENBANK/X54745 GENBANK/X56575 GENBANK/X56576 GENBANK/X56577 GENBANK/X56578 GENBANK/X56579 GENBANK/X56580 GENBANK/X56581 GENBANK/X56582 GENBANK/X56583 Base Sequence Molecular Sequence Data Phylogeny RNA Probes RNA, Bacterial/*chemistry RNA, Ribosomal, 16S/*chemistry Support, Non-U.S. Gov't Vibrio/*classification/genetics/isolation & purification
Remarks: The inter- and intrageneric relationships of the genus Vibrio were investigated by performing a comparative analysis of the 16S rRNAs of 10 species, including four pathogenic representatives. The results of immunological and 5S rRNA studies were confirmed in that the genus is a neighboring taxon of the family Enterobacteriaceae. With regard to the intrageneric structure, Vibrio alginolyticus, Vibrio campbellii, Vibrio natriegens, Vibrio harveyi, Vibrio proteolyticus, Vibrio parahaemolyticus, and Vibrio vulnificus form the core of the genus, while Vibrio (Listonella) anguillarum, Vibrio diazotrophicus, and Vibrio hollisae are placed on the outskirts of the genus. Variable regions around positions 80, 180, and 450 could be used as target sites for genus- and species-specific oligonucleotide probes and polymerase chain reaction primers to be used in molecular identification.
URL: 92144377
Ref #: 13613
Author(s): Lee,S.K.;Wang,H.Z.;Law,S.H.;Wu,R.S.;Kong,R.Y.
Journal: Mar Pollut Bull
Title: Analysis of the 16S-23S rDNA intergenic spacers (IGSs) of marine vibrios for species-specific signature DNA sequences
Volume: 44
Page(s): 412-20
Year: 2002
Remarks: Vibrios are widespread in the marine environment and a few pathogenic species are known to be commonly associated with outbreaks of diarrheal diseases in humans due to the consumption of raw or improperly cooked seafood. However, there are also many Vibrio species which are potentially pathogenic to vertebrate and invertebrate aquatic animals, and of which little is known. In an attempt to develop rapid PCR detection methods for these latter class of vibrios, we have examined the 16S-23S intergenic spacers (IGSs) of 10 lesser-known Vibrio species and successfully developed species-specific primers for eight of them--Vibrio costicola, V. diazotrophicus, V. fluvialis, V. nigripulchritudo, V. proteolyticus, V. salmonicida, V. splendidus and V. tubiashii. The IGS amplicons were amplified using primers complementary to conserved regions of the 16S and 23S rRNA genes, and cloned into plasmid vectors and sequenced. Analysis of the IGS sequences showed that 37 ribosomal RNA (rrn) operons representing seven different IGS types have been cloned from the 10 vibrios. The three IGS types--IGS(0), IGS(IA) and IGS(Glu)--were the most prevalent forms detected. Multiple alignment of representative sequences of these three IGS types from different Vibrio species revealed several domains of high sequence variability, which were used to design species-specific primers for PCR. The specificity of the primers were evaluated using total DNA prepared from different Vibrio species and bacterial genera. The results showed that the PCR method can be used to reliably detect eight of the 10 Vibrio species in marine waters in this study.
URL: 22141388
Data: Type strain / ATCC in 1979 / P. Baumann / Seawater / Baumann, P. et al. (1971) J. Bact. 107, 289
Accession Date: 01/01/1979
History: ATCC,ISOLATED BY GUNDERSON K 1969
Authority: (Baumann et al. 1971) Baumann et al. 1981
Depositor: GHERNA R L
Taxonomy: TaxLink: S3539 (Beneckea campbellii) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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