Culture Collections

Bacteria and Mycoplasmas detail

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Bacteria Collection: Nocardia asteroides

NCTC Number: NCTC 11293
Current Name: Nocardia asteroides
Original Strain Reference: R. E. Gordon 727
Other Collection No: ATCC 19247; CCM 2754; DSM 43373; DSM 43757; IMET 7547; JCM 3384; R E GORDON 727
Previous Catalogue Name: Nocardia asteroides
Type Strain: Yes
Family: Nocardiaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48 hours, 30°C, aerobic
Conditions for growth on liquid media: nutrient broth,30, aerobic
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1497486
16S rRNA Gene Sequence: >gb|X84850|ATCC 19247T|N.asteroides 16S rRNA gene (ATCC 19247T).| acgaacgctggcggc... >gb|X80606|DSM43757T|N.asteroides 16S rDNA.| gctcaggacgaacgc... >gb|Z36934|M. GOODFELLOW N317/ATCC 19247(TYPE STRAIN)|N.asteroides gene for 16S ribosomal RNA.| gacgaacgctggcgg... >gb|DQ659898|ATCC 19247|Nocardia asteroides strain ATCC 19247 16S ribosomal RNA gene,partial sequence.| cgaacgctggcggcg... >gb|AY756541|ATCC 19247|Nocardia asteroides strain ATCC 19247 16S ribosomal RNA gene,partial sequence.| gcttaacacatgcaa... >gb|AY505492|ATCC 19247|Nocardia asteroides strain ATCC 19247 16S ribosomal RNA gene,partial sequence.| tggagagtttgatcc... >gb|AF536395|DSM 43757|Nocardia asteroides strain DSM 43757 16S-23S ribosomal RNAintergenic spacer, complete sequence.| aaggagcattctcca... >gb|AF536394|DSM 43757|Nocardia asteroides strain DSM 43757 rrnA operon 16S-23S ribosomalRNA intergenic spacer, complete sequence.| aaggagcatctgcat... >gb|AF536393|DSM 43757|Nocardia asteroides strain DSM 43757 rrnB operon 16S-23S ribosomalRNA intergenic spacer, complete sequence.| aaggagcacatctcc...
23S rRNA Gene Sequence: >gb|AF536395|DSM 43757|Nocardia asteroides strain DSM 43757 16S-23S ribosomal RNAintergenic spacer, complete sequence.| aaggagcattctcca... >gb|AF536394|DSM 43757|Nocardia asteroides strain DSM 43757 rrnA operon 16S-23S ribosomalRNA intergenic spacer, complete sequence.| aaggagcatctgcat... >gb|AF536393|DSM 43757|Nocardia asteroides strain DSM 43757 rrnB operon 16S-23S ribosomalRNA intergenic spacer, complete sequence.| aaggagcacatctcc...
Bibliography: GORDON R E & MIHM J M 1957 J BACT 73 15 27
Extended Bibliography: showhide Show bibliography
Ref #: 65940
Author(s): Ruimy,R.;Riegel,P.;Carlotti,A.;Boiron,P.;Bernardin,G.;Monteil,H.;Wallace RJ,J.r.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Nocardia pseudobrasiliensis sp. nov., a new species of Nocardia which groups bacterial strains previously identified as Nocardia brasiliensis and associated with invasive diseases
Volume: 46
Page(s): 259-64
Year: 1996
Keyword(s): GENBANK/X84850 GENBANK/X84851 GENBANK/X84852 GENBANK/X84853 GENBANK/X84854 GENBANK/X84855 GENBANK/X84856 GENBANK/X84857 DNA, Bacterial/genetics DNA, Ribosomal/genetics Molecular Sequence Data Nocardia/chemistry/*classification/genetics/metabolism Nocardia Infections/*microbiology Nucleic Acid Hybridization Phylogeny
Remarks: We studied five strains of a new Nocardia taxon recently identified among Nocardia brasiliensis strains associated with invasive diseases (R. J. Wallace, Jr., B. A. Brown, Z. Blacklock, R. Ulrich, K. Jost, J. M. Brown, M. M. McNeil, G. Onyi, V. A. Steingrube, and J. Gibson, J. Clin. Microbiol. 33:1528-1533, 1995) to determine their taxonomic status. Several characteristics of these organisms, including the presence of chemotype IV cell walls, nocardomycolic acids, a predominant menaquinone similar to that of Nocardia asteroides ATCC 19247T (T = type strain), and G+C contents ranging from 67 to 68 mol%, are characteristics of the genus Nocardia. Phylogenies based on small-subunit ribosomal DNA sequences clearly confirmed that all five strains belong to the genus Nocardia and occur on a single branch that is clearly distinct from N. brasiliensis. This branch forms a clade with Nocardia vaccinii, Nocardia nova, Nocardia otitidiscaviarum, and Nocardia seriolae. The five new strains exhibited high levels of DNA relatedness with each other, as determined by DNA-DNA hybridization experiments (S1 nuclease procedure), but not with N. brasiliensis strains or with strains of the four phylogenetically related Nocardia species mentioned above. The five new strains differ from N. brasiliensis in the following characteristics: mycolic acid pattern, decomposition of adenine, nitrate reduction, and antimicrobial agent susceptibilities. Therefore, we propose that these strains belong to a new species, Nocardia pseudobrasiliensis. The type strain is strain ATCC 51512, which was isolated from a leg abscess on a patient suffering from ulcerative colitis.
URL: 8573505
Ref #: 65647
Author(s): Patel,J.B.;Wallace RJ,J.r.;Brown-Elliott,B.A.;Taylor,T.;Imperatrice,C.;Leonard,D.G.;Wilson,R.W.;Mann,L.;Jost,K.C.;Nachamkin,I.
Journal: J Clin Microbiol
Title: Sequence-based identification of aerobic actinomycetes
Volume: 42
Page(s): 2530-40
Year: 2004
Keyword(s): Actinobacteria/classification/genetics/*isolation & purification Aerobiosis DNA, Ribosomal/chemistry Nocardia/genetics/isolation & purification Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA
Remarks: We investigated the utility of 500-bp 16S rRNA gene sequencing for identifying clinically significant species of aerobic actinomycetes. A total of 28 reference strains and 71 clinical isolates that included members of the genera Streptomyces, Gordonia, and Tsukamurella and 10 taxa of Nocardia were studied. Methods of nonsequencing analyses included growth and biochemical analysis, PCR-restriction enzyme analysis of the 439-bp Telenti fragment of the 65 hsp gene, susceptibility testing, and, for selected isolates, high-performance liquid chromatography. Many of the isolates were included in prior taxonomic studies. Sequencing of Nocardia species revealed that members of the group were generally most closely related to the American Type Culture Collection (ATCC) type strains. However, the sequences of Nocardia transvalensis, N. otitidiscaviarum, and N. nova isolates were highly variable; and it is likely that each of these species contains multiple species. We propose that these three species be designated complexes until they are more taxonomically defined. The sequences of several taxa did not match any recognized species. Among other aerobic actinomycetes, each group most closely resembled the associated reference strain, but with some divergence. The study demonstrates the ability of partial 16S rRNA gene sequencing to identify members of the aerobic actinomycetes, but the study also shows that a high degree of sequence divergence exists within many species and that many taxa within the Nocardia spp. are unnamed at present. A major unresolved issue is the type strain of N. asteroides, as the present one (ATCC 19247), chosen before the availability of molecular analysis, does not represent any of the common taxa associated with clinical nocardiosis.
URL: 15184431
Ref #: 95453
Author(s): Rodriguez-Nava,V.;Couble,A.;Devulder,G.;Flandrois,J.P.;Boiron,P.;Laurent,F.
Journal: J Clin Microbiol
Title: Use of PCR-restriction enzyme pattern analysis and sequencing database for hsp65 gene-based identification of Nocardia species
Volume: 44
Page(s): 536-46
Year: 2006
Keyword(s): Animals Bacterial Proteins/genetics *Bacterial Typing Techniques Databases, Genetic Genes, rRNA Heat-Shock Proteins/*genetics Humans Nocardia/*classification/genetics Phylogeny Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics *Restriction Mapping Sequence Analysis, DNA Species Specificity
Remarks: Nocardia identification required laborious and time-consuming phenotypic and chemotaxonomic methods until molecular methods were developed in the mid-1990s. Here we reassessed the capacity of PCR-restriction enzyme pattern analysis (PRA) of the hsp65 gene to differentiate Nocardia species, including 36 new species. Our results confirm that hsp65 PRA must no longer be used for Nocardia species identification, as many species have the same restriction pattern. We then compared sequencing-based strategies using an hsp65 database and a 16S rRNA database and found that the hsp65 region contained sufficient polymorphisms for comprehensive Nocardia species identification.
URL: 16455910
Ref #: 65999
Author(s): Chun,J.;Goodfellow,M.
Journal: Int J Syst Bacteriol
Title: A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences
Volume: 45
Page(s): 240-5
Year: 1995
Keyword(s): GENBANK/Z36925 GENBANK/Z36926 GENBANK/Z36927 GENBANK/Z36928 GENBANK/Z36929 GENBANK/Z36930 GENBANK/Z36933 GENBANK/Z36934 GENBANK/Z36935 GENBANK/Z36936 Base Sequence DNA, Bacterial/+ACo-genetics Genes, Bacterial Molecular Sequence Data Nocardia/chemistry/+ACo-classification/genetics Phylogeny RNA, Bacterial/+ACo-genetics RNA, Ribosomal, 16S/+ACo-genetics Rhodococcus/classification/genetics Sequence Analysis Vitamin K/analysis
Remarks: Partial sequences of the 16S rRNA genes of the type strains of nine species of the genus Nocardia were determined following the isolation and cloning of the amplified genes. These sequences were aligned with the sequences of representatives of the genera Corynebacterium, Gordona, Mycobacterium, Rhodococcus, and Tsukamurella, and phylogenetic trees were inferred by using the Fitch-Margoliash and neighbor-joining methods. The genus Nocardia formed a distinct clade that was most closely associated with the genus Rhodococcus. The average level of sequence similarity found among the type strains of the Nocardia species was 97.2 0.7+ACU-. Two sublines were recognized within the Nocardia clade+ADs- one encompassed Nocardia asteroides and related species, and the other encompassed Nocardia otitidiscaviarum and allied taxa. Separation of the two sublines is based on differences in helix 37-1. The results of isoprenoid quinone analyses provided evidence that nocardiae can be distinguished from all other actinomycete taxa on the basis of their characteristic menaquinone profiles. Nocardiae typically contain hexahydrogenated menaquinones with eight isoprene units in which the two end units are cyclized.
URL: 7537058
Ref #: 95438
Author(s): Conville,P.S.;Zelazny,A.M.;Witebsky,F.G.
Journal: J Clin Microbiol
Title: Analysis of secA1 gene sequences for identification of Nocardia species
Volume: 44
Page(s): 2760-6
Year: 2006
Keyword(s): GENBANK/DQ360260 GENBANK/DQ360261 GENBANK/DQ360262 GENBANK/DQ360263 GENBANK/DQ360265 GENBANK/DQ360266 GENBANK/DQ360267 GENBANK/DQ360268 GENBANK/DQ360269 GENBANK/DQ360270 GENBANK/DQ360271 GENBANK/DQ360272 GENBANK/DQ360273 GENBANK/DQ360274 GENBANK/DQ360275 GENBANK/DQ360276 GENBANK/DQ360277 GENBANK/DQ360278 GENBANK/DQ360279 GENBANK/DQ360280 GENBANK/DQ360281 GENBANK/DQ360282 GENBANK/DQ360284 GENBANK/DQ360285 GENBANK/DQ360286 GENBANK/DQ360287 GENBANK/DQ360288 GENBANK/DQ360289 GENBANK/DQ360290 GENBANK/DQ366276 GENBANK/DQ659895 GENBANK/DQ659896 GENBANK/DQ659897 GENBANK/DQ659898 GENBANK/DQ659899 GENBANK/DQ659900 GENBANK/DQ659901 GENBANK/DQ659902 GENBANK/DQ659903 GENBANK/DQ659904 GENBANK/DQ659905 GENBANK/DQ659906 GENBANK/DQ659907 GENBANK/DQ659908 GENBANK/DQ659909 GENBANK/DQ659910 GENBANK/DQ659911 GENBANK/DQ659912 GENBANK/DQ659913 GENBANK/DQ659914 GENBANK/DQ659915 GENBANK/DQ659916 GENBANK/DQ659917 GENBANK/DQ659918 GENBANK/DQ659919 GENBANK/DQ659920 Adenosine Triphosphatases/*genetics Bacterial Proteins/*genetics Cluster Analysis DNA, Bacterial/chemistry/*genetics DNA, Ribosomal/genetics Humans Membrane Transport Proteins/*genetics Molecular Sequence Data Nocardia/*classification/*genetics Phylogeny RNA, Ribosomal, 16S/genetics *Sequence Analysis, DNA Sequence Homology
Remarks: Molecular methodologies, especially 16S rRNA gene sequence analysis, have allowed the recognition of many new species of Nocardia and to date have been the most precise methods for identifying isolates reliably to the species level. We describe here a novel method for identifying Nocardia isolates by using sequence analysis of a portion of the secA1 gene. A region of the secA1 gene of 30 type or reference strains of Nocardia species was amplified using secA1-specific primers. Sequence analysis of 468 bp allowed clear differentiation of all species, with a range of interspecies similarity of 85.0% to 98.7%. Corresponding 16S rRNA gene sequences of a 1,285-bp region for the same isolates showed a range of interspecies similarity of 94.4 to 99.8%. In addition to the type and reference strains, a 468-bp fragment of the secA1 gene was sequenced from 40 clinical isolates of 12 Nocardia species previously identified by 16S rRNA gene sequence analysis. The secA1 gene sequences of most isolates showed >99.0% similarity to the secA1 sequences of the type or reference strain to which their identification corresponded, with a range of 95.3 to 100%. Comparison of the deduced 156 amino acid sequences of the SecA1 proteins of the clinical isolates showed between zero and two amino acid residue differences compared to that of the corresponding type or reference strain. Sequencing of the secA1 gene, and using deduced amino acid sequences of the SecA1 protein, may provide a more discriminative and precise method for the identification of Nocardia isolates than 16S rRNA gene sequencing.
URL: 16891489
Ref #: 13292
Author(s): Chun,J.;Goodfellow,M.
Journal: Int J Syst Bacteriol
Title: A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences
Volume: 45
Page(s): 240-5
Year: 1995
Keyword(s): GENBANK/Z36925 GENBANK/Z36926 GENBANK/Z36927 GENBANK/Z36928 GENBANK/Z36929 GENBANK/Z36930 GENBANK/Z36933 GENBANK/Z36934 GENBANK/Z36935 GENBANK/Z36936 Base Sequence DNA, Bacterial/+ACo-genetics Genes, Bacterial Molecular Sequence Data Nocardia/chemistry/+ACo-classification/genetics Phylogeny RNA, Bacterial/+ACo-genetics RNA, Ribosomal, 16S/+ACo-genetics Rhodococcus/classification/genetics Sequence Analysis Vitamin K/analysis
Remarks: Partial sequences of the 16S rRNA genes of the type strains of nine species of the genus Nocardia were determined following the isolation and cloning of the amplified genes. These sequences were aligned with the sequences of representatives of the genera Corynebacterium, Gordona, Mycobacterium, Rhodococcus, and Tsukamurella, and phylogenetic trees were inferred by using the Fitch-Margoliash and neighbor-joining methods. The genus Nocardia formed a distinct clade that was most closely associated with the genus Rhodococcus. The average level of sequence similarity found among the type strains of the Nocardia species was 97.2 0.7+ACU-. Two sublines were recognized within the Nocardia clade+ADs- one encompassed Nocardia asteroides and related species, and the other encompassed Nocardia otitidiscaviarum and allied taxa. Separation of the two sublines is based on differences in helix 37-1. The results of isoprenoid quinone analyses provided evidence that nocardiae can be distinguished from all other actinomycete taxa on the basis of their characteristic menaquinone profiles. Nocardiae typically contain hexahydrogenated menaquinones with eight isoprene units in which the two end units are cyclized.
URL: 95244306
Ref #: 13291
Author(s): Ruimy,R.;Riegel,P.;Carlotti,A.;Boiron,P.;Bernardin,G.;Monteil,H.;Wallace RJ,J.r.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Nocardia pseudobrasiliensis sp. nov., a new species of Nocardia which groups bacterial strains previously identified as Nocardia brasiliensis and associated with invasive diseases
Volume: 46
Page(s): 259-64
Year: 1996
Keyword(s): GENBANK/X84850 GENBANK/X84851 GENBANK/X84852 GENBANK/X84853 GENBANK/X84854 GENBANK/X84855 GENBANK/X84856 GENBANK/X84857 DNA, Bacterial/genetics DNA, Ribosomal/genetics Molecular Sequence Data Nocardia/chemistry/*classification/genetics/metabolism Nocardia Infections/*microbiology Nucleic Acid Hybridization Phylogeny Support, Non-U.S. Gov't
Remarks: We studied five strains of a new Nocardia taxon recently identified among Nocardia brasiliensis strains associated with invasive diseases (R. J. Wallace, Jr., B. A. Brown, Z. Blacklock, R. Ulrich, K. Jost, J. M. Brown, M. M. McNeil, G. Onyi, V. A. Steingrube, and J. Gibson, J. Clin. Microbiol. 33:1528-1533, 1995) to determine their taxonomic status. Several characteristics of these organisms, including the presence of chemotype IV cell walls, nocardomycolic acids, a predominant menaquinone similar to that of Nocardia asteroides ATCC 19247T (T = type strain), and G+C contents ranging from 67 to 68 mol%, are characteristics of the genus Nocardia. Phylogenies based on small-subunit ribosomal DNA sequences clearly confirmed that all five strains belong to the genus Nocardia and occur on a single branch that is clearly distinct from N. brasiliensis. This branch forms a clade with Nocardia vaccinii, Nocardia nova, Nocardia otitidiscaviarum, and Nocardia seriolae. The five new strains exhibited high levels of DNA relatedness with each other, as determined by DNA-DNA hybridization experiments (S1 nuclease procedure), but not with N. brasiliensis strains or with strains of the four phylogenetically related Nocardia species mentioned above. The five new strains differ from N. brasiliensis in the following characteristics: mycolic acid pattern, decomposition of adenine, nitrate reduction, and antimicrobial agent susceptibilities. Therefore, we propose that these strains belong to a new species, Nocardia pseudobrasiliensis. The type strain is strain ATCC 51512, which was isolated from a leg abscess on a patient suffering from ulcerative colitis.
URL: 96138987
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 6413
Journal: J. Bacteriol.
Volume: 127
Page(s): 584-594
Year: 1976
Ref #: 755
Author(s): Gordon,R.E.;Mihm,J.M.
Journal: J. Gen. Microbiol.
Title: A comparison of Nocardia asteroides and Nocardia brasiliensis.
Volume: 20
Page(s): 129-135
Year: 1959
Data: (ATCC 19247) Type strain / ATCC in 1979 / Gordon, R. E. and Mihm, J. M. (1957) J. Bact. 73, 15-27
Accession Date: 01/01/1979
Authority: (Eppinger 1891) Blanchard 1896 (AL)
Depositor: GHERNA R L, ATCC
Taxonomy: TaxLink: S2136 (Nocardia asteroides (Eppinger 1891) Blanchard 1896) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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