Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Legionella pneumophila

NCTC Number: NCTC 11286
Current Name: Legionella pneumophila
Original Strain Reference: Knoxville-1
Other Collection No: ATCC 33153; KNOXVILLE-1
Previous Catalogue Name: Legionella pneumophila
Type Strain: No
Family: Legionellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Buffered Charcoal Yeast Extract agar (BCYE), 48 hours, 37°C, requires carbon dioxide
Isolated From: human, lung
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1211137
23S rRNA Gene Sequence: >gb|Z30432|KNOXVILLE-1|ATCC 33153|L.pneumophila Serogroup 1 Knoxville-1 genes for 23S ribosomal RNAand 5S ribosomal RNA.| gcctccctcaagatg... >gb|Z24702|KNOXVILLE-1|ATCC 33153|L.pneumophila 23S and 5S rRNA genes, partial CDSs.| gcctccctcaagatg... >gb|Z24702|KNOXVILLE-1|ATCC 33153|L.pneumophila 23S and 5S rRNA genes, partial CDS's.| gcctccctcaagatg...
Bibliography: BRENNER D J ET AL 1979 ANN INT MED 90 656
Extended Bibliography: showhide Show bibliography
Ref #: 54193
Author(s): Ko,K.S.;Lee,H.K.;Park,M.Y.;Park,M.S.;Lee,K.H.;Woo,S.Y.;Yun,Y.J.;Kook,Y.H.
Journal: J Bacteriol
Title: Population genetic structure of Legionella pneumophila inferred from RNA polymerase gene (rpoB) and DotA gene (dotA) sequences
Volume: 184
Page(s): 2123-30
Year: 2002
Keyword(s): Amino Acid Sequence *Bacterial Proteins Base Sequence Carrier Proteins/*genetics DNA-Directed RNA Polymerases/*genetics Legionella pneumophila/*classification/genetics Membrane Proteins/*genetics Molecular Sequence Data Serotyping
Remarks: The population structure of Legionella pneumophila was studied by using partial RNA polymerase gene (rpoB) and DotA gene (dotA) sequences. Trees inferred from rpoB sequences showed that two subspecies of L. pneumophila, Legionella pneumophila subsp. pneumophila and Legionella pneumophila subsp. fraseri, were clearly separated genetically. In both rpoB and dotA trees, 79 Korean isolates used in this study constituted six clonal populations, four of which (designated subgroups P-I to P-IV) were identified in L. pneumophila subsp. pneumophila and two of which (designated subgroups F-I and F-II) were identified in L. pneumophila subsp. fraseri. Although the relationships among subgroups were not identical, such subgrouping was congruent between the rpoB and dotA trees. Type strains of several serogroups did not belong to any subgroup, presumably because isolates similar to these strains were not present among our local sample of the population. There was evidence that horizontal gene transfer or recombination had occurred within L. pneumophila. Contrary to the phylogeny from rpoB and the taxonomic context, subgroups P-III and P-IV of L. pneumophila subsp. pneumophila proved to be closely related to those of L. pneumophila subsp. fraseri or showed a distinct clustering in the dotA tree. It can be inferred that dotA of subgroups P-III and P-IV has been transferred horizontally from other subspecies. The diverse distribution of serogroup 1 strains through the gene trees suggests that surface antigen-coding genes that determine serogroup can be exchanged. Thus, it can be inferred that genetic recombination has been important in the evolution of L. pneumophila.
URL: 11914343
Ref #: 95439
Author(s): Robinson,P.N.;Heidrich,B.;Tiecke,F.;Fehrenbach,F.J.;Rolfs,A.
Journal: FEMS Microbiol Lett
Title: Species-specific detection of Legionella using polymerase chain reaction and reverse dot-blotting
Volume: 140
Page(s): 111-9
Year: 1996
Keyword(s): GENBANK/Z30431 GENBANK/Z30432 GENBANK/Z30433 GENBANK/Z30434 GENBANK/Z30435 GENBANK/Z30436 GENBANK/Z30437 GENBANK/Z30438 GENBANK/Z30439 GENBANK/Z30440 GENBANK/Z30441 GENBANK/Z30442 GENBANK/Z30443 GENBANK/Z30444 GENBANK/Z30445 GENBANK/Z30446 GENBANK/Z30447 GENBANK/Z30448 GENBANK/Z30449 GENBANK/Z30450 GENBANK/Z30451 GENBANK/Z30452 GENBANK/Z30453 GENBANK/Z30454 GENBANK/Z30455 GENBANK/Z30534 GENBANK/Z30535 GENBANK/Z30536 GENBANK/Z30537 GENBANK/Z30538 etc. Base Sequence DNA Primers/genetics DNA Probes/genetics DNA, Bacterial/*genetics/isolation & purification DNA, Ribosomal/genetics Humans Legionella/classification/*genetics/isolation & purification Legionnaires' Disease/diagnosis/microbiology Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics RNA, Ribosomal, 5S/genetics Sequence Homology, Nucleic Acid Species Specificity
Remarks: Legionella pneumophila and some other Legionella species are capable of causing Legionnaire's disease, a potentially fatal pneumonia. The identification of legionellae by standard laboratory techniques such as culture is difficult and time consuming. In the present work, the DNA sequence of the 23S-5S spacer region was determined for 43 Legionella isolates, and the sequence information was used to develop a species-specific detection system using PCR and reverse dot-blotting which employs just one PCR amplicon to perform genus- and species-specific detection. L. pneumophila serogroups 1-16 as well as 21 non-pneumophila isolates could be identified and differentiated at the species level using this system.
URL: 8764471
Ref #: 13685
Author(s): Robinson,P.N.;Heidrich,B.;Tiecke,F.;Fehrenbach,F.J.;Rolfs,A.
Journal: FEMS Microbiol Lett
Title: Species-specific detection of Legionella using polymerase chain reaction
Volume: 140
Page(s): 111-119
Year: 1996
Keyword(s): 0 (DNA Primers) 0 (DNA Probes) 0 (DNA, Bacterial) 0 (DNA, Ribosomal) 0 (RNA, Bacterial) 0 (RNA, Ribosomal, 23S) 0 (RNA, Ribosomal, 5S) Base Sequence Comparative Study DNA Primers/genetics DNA Probes/genetics DNA, Bacterial/*genetics/isolation & purification DNA, Ribosomal/genetics Human Legionella/classification/*genetics/isolation & purification Legionnaires' Disease/diagnosis/microbiology Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics RNA, Ribosomal, 5S/genetics Sequence Homology, Nucleic Acid Species Specificity
Remarks: Legionella pneumophila and some other Legionella species are capable of
URL: 96291654
Data: (ATCC 33153) R. E. Weaver, CDC Atlanta in 1980 / J. E. McDade / Human lung / Serogroup 1 / Brenner, D. G. et al. (1979) Ann. Int. Med. 90, 656
Accession Date: 01/01/1980
History: WEAVER R E, CDC ATLANTA-ISLT BY MCDADE J E, CDC ATLANTA
Authority: Brenner et al. 1979 (AL)
Depositor: WEAVER R E
Taxonomy: TaxLink: S1650 (Legionella pneumophila subsp. pneumophila Brenner et al. 1979) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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