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Bacteria Collection: Moraxella (branhamella) ovis

NCTC Number: NCTC 11227
Current Name: Moraxella (branhamella) ovis
Original Strain Reference: 199/55
Other Collection No: ATCC 33078; NEISSERIA OVIS; 199/55
Previous Catalogue Name: Moraxella ovis
Type Strain: Yes
Family: Moraxellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Chocolate blood agar, 48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, aerobic
Isolated From: mammal, ovine infectious keratonconjuctivitis
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1247844
16S rRNA Gene Sequence: >gb|X74896|NCTC 11227T|N.ovis gene for 16S rRNA.| aggcttaacacatgc... >gb|AF005186|ATCC 33078T|Moraxella ovis 16S ribosomal RNA gene, partial sequence.| ctggcggcaggctta... >gb|DQ647928|ATCC 33078|Moraxella ovis 16S ribosomal RNA gene, partial sequence; 16S-23Sintergenic spacer, complete sequence; and 23S ribosomal RNA gene,partial sequence.| gaacgatgagtatcc...
23S rRNA Gene Sequence: >gb|DQ647928|ATCC 33078|Moraxella ovis 16S ribosomal RNA gene, partial sequence; 16S-23Sintergenic spacer, complete sequence; and 23S ribosomal RNA gene,partial sequence.| gaacgatgagtatcc...
Bibliography: BOVRE K ET AL 1969 CANAD J MICROBIOL 15 335; JANTZEN E ET AL 1974 ACTA
Extended Bibliography: showhide Show bibliography
Ref #: 58671
Author(s): Pettersson,B.;Kodjo,A.;Ronaghi,M.;Uhlen,M.;Tonjum,T.
Journal: Int J Syst Bacteriol
Title: Phylogeny of the family Moraxellaceae by 16S rDNA sequence analysis, with special emphasis on differentiation of Moraxella species
Volume: 48 Pt 1
Page(s): 75-89
Year: 1998
Keyword(s): GENBANK/AF005160 GENBANK/AF005161 GENBANK/AF005162 GENBANK/AF005163 GENBANK/AF005164 GENBANK/AF005165 GENBANK/AF005166 GENBANK/AF005167 GENBANK/AF005168 GENBANK/AF005169 GENBANK/AF005170 GENBANK/AF005171 GENBANK/AF005172 GENBANK/AF005173 GENBANK/AF005174 GENBANK/AF005175 GENBANK/AF005176 GENBANK/AF005177 GENBANK/AF005178 GENBANK/AF005179 GENBANK/AF005180 GENBANK/AF005181 GENBANK/AF005182 GENBANK/AF005183 GENBANK/AF005184 GENBANK/AF005185 GENBANK/AF005186 GENBANK/AF005187 GENBANK/AF005188 GENBANK/AF005189 etc. DNA Primers DNA, Bacterial/analysis Molecular Sequence Data Moraxella (Branhamella) catarrhalis/*chemistry/*genetics Moraxella (Moraxella) bovis/*classification/*genetics Oligonucleotides Phylogeny RNA, Bacterial/analysis RNA, Ribosomal, 16S/*analysis Species Specificity
Remarks: Thirty-three strains previously classified into 11 species in the bacterial family Moraxellaceae were subjected to phylogenetic analysis based on 16S rRNA sequences. The family Moraxellaceae formed a distinct clade consisting of four phylogenetic groups as judged from branch lengths, bootstrap values and signature nucleotides. Group I contained the classical moraxellae and strains of the coccal moraxellae, previously known as Branhamella, with 16S rRNA similarity of > or = 95%. A further division of group I into five tentative clusters is discussed. Group II consisted of two strains representing Moraxella atlantae and Moraxella osloensis. These strains were only distantly related to each other (93.4%) and also to the other members of the Moraxellaceae (< or = 93%). Therefore, reasons for reclassification of these species into separate and new genera are discussed. Group III harboured strains of the genus Psychrobacter and strain 752/52 of [Moraxella] phenylpyruvica. This strain of [M.] phenylpyruvica formed an early branch from the group III line of descent. Interestingly, a distant relationship was found between Psychrobacter phenylpyruvicus strain ATCC 23333T (formerly classified as [M.] phenylpyruvica) and [M.] phenylpyruvica strain 752/52, exhibiting less than 96% nucleotide similarity between their 16S rRNA sequences. The establishment of a new genus for [M.] phenylpyruvica strain 752/52 is therefore suggested. Group IV contained only two strains of the genus Acinetobacter. Strategies for the development of diagnostic probes and distinctive sequences for 16S rRNA-based species-specific assays within group I are suggested. Although these findings add to the classificatory placements within the Moraxellaceae, analysis of a more comprehensive selection of strains is still needed to obtain a complete classification system within this family.
URL: 9542079
Ref #: 58678
Author(s): Angelos,J.A.;Spinks,P.Q.;Ball,L.M.;George,L.W.
Journal: Int J Syst Evol Microbiol
Title: Moraxella bovoculi sp. nov., isolated from calves with infectious bovine keratoconjunctivitis
Volume: 57
Page(s): 789-95
Year: 2007
Keyword(s): GENBANK/DQ153081 GENBANK/DQ153082 GENBANK/DQ153083 GENBANK/DQ153084 GENBANK/DQ153085 GENBANK/DQ153086 GENBANK/DQ153087 GENBANK/DQ153088 GENBANK/DQ153089 GENBANK/DQ153090 GENBANK/DQ153091 GENBANK/DQ153092 GENBANK/DQ153093 GENBANK/DQ153094 GENBANK/DQ153095 GENBANK/DQ153096 GENBANK/DQ153097 GENBANK/DQ153098 GENBANK/DQ156147 GENBANK/DQ156148 GENBANK/DQ647927 GENBANK/DQ647928
Remarks: Eighteen isolates of a Gram-negative coccus (strain 237(T)) were cultured from the eyes of dairy and beef calves affected with infectious bovine keratoconjunctivitis (IBK; 'pinkeye') in northern California, USA, during summer 2002. These isolates had near full-length (1397 bp) 16S rRNA gene sequences that clustered into three groups with 99.9 % sequence similarity. On the basis of 16S rRNA gene sequence, the isolates were most closely associated with Moraxella bovis and Moraxella ovis in clade I of the classical moraxellae. Biochemically, the novel isolates could be distinguished from the other members of the genus Moraxella isolated from animals on the basis of phenylalanine deaminase activity. The results of partial sequence analysis of six housekeeping genes, the 16S-23S rRNA gene interspacer region and partial 23S rRNA gene provide strong support for the inclusion of these isolates in a novel taxon, for which the name Moraxella bovoculi sp. nov. is proposed. The type strain is strain 237(T) (=ATCC BAA-1259(T)=CCUG 52049(T)).
URL: 17392208
Ref #: 13137
Author(s): Pettersson,B.;Kodjo,A.;Ronaghi,M.;Uhlen,M.;Tonjum,T.
Journal: Int J Syst Bacteriol
Title: Phylogeny of the family Moraxellaceae by 16S rDNA sequence analysis, with special emphasis on differentiation of Moraxella species
Volume: 48 Pt 1
Page(s): 75-89
Year: 1998
Keyword(s): GENBANK/AF005160 GENBANK/AF005161 GENBANK/AF005162 GENBANK/AF005163 GENBANK/AF005164 GENBANK/AF005165 GENBANK/AF005166 GENBANK/AF005167 GENBANK/AF005168 GENBANK/AF005169 GENBANK/AF005170 GENBANK/AF005171 GENBANK/AF005172 GENBANK/AF005173 GENBANK/AF005174 GENBANK/AF005175 GENBANK/AF005176 GENBANK/AF005177 GENBANK/AF005178 GENBANK/AF005179 GENBANK/AF005180 GENBANK/AF005181 GENBANK/AF005182 GENBANK/AF005183 GENBANK/AF005184 GENBANK/AF005185 GENBANK/AF005186 GENBANK/AF005187 GENBANK/AF005188 GENBANK/AF005189 etc. DNA Primers DNA, Bacterial/analysis Molecular Sequence Data Moraxella (Branhamella) catarrhalis/*chemistry/*genetics Moraxella (Moraxella) bovis/*classification/*genetics Oligonucleotides Phylogeny RNA, Bacterial/analysis RNA, Ribosomal, 16S/*analysis Species Specificity Support, Non-U.S. Gov't
Remarks: Thirty-three strains previously classified into 11 species in the bacterial family Moraxellaceae were subjected to phylogenetic analysis based on 16S rRNA sequences. The family Moraxellaceae formed a distinct clade consisting of four phylogenetic groups as judged from branch lengths, bootstrap values and signature nucleotides. Group I contained the classical moraxellae and strains of the coccal moraxellae, previously known as Branhamella, with 16S rRNA similarity of > or = 95%. A further division of group I into five tentative clusters is discussed. Group II consisted of two strains representing Moraxella atlantae and Moraxella osloensis. These strains were only distantly related to each other (93.4%) and also to the other members of the Moraxellaceae (< or = 93%). Therefore, reasons for reclassification of these species into separate and new genera are discussed. Group III harboured strains of the genus Psychrobacter and strain 752/52 of [Moraxella] phenylpyruvica. This strain of [M.] phenylpyruvica formed an early branch from the group III line of descent. Interestingly, a distant relationship was found between Psychrobacter phenylpyruvicus strain ATCC 23333T (formerly classified as [M.] phenylpyruvica) and [M.] phenylpyruvica strain 752/52, exhibiting less than 96% nucleotide similarity between their 16S rRNA sequences. The establishment of a new genus for [M.] phenylpyruvica strain 752/52 is therefore suggested. Group IV contained only two strains of the genus Acinetobacter. Strategies for the development of diagnostic probes and distinctive sequences for 16S rRNA-based species-specific assays within group I are suggested. Although these findings add to the classificatory placements within the Moraxellaceae, analysis of a more comprehensive selection of strains is still needed to obtain a complete classification system within this family.
URL: 98203182
Ref #: 11987
Author(s): Enright,M.C.;Carter,P.E.;MacLean,I.A.;McKenzie,H.
Journal: Int J Syst Bacteriol
Title: Phylogenetic relationships between some members of the genera Neisseria, Acinetobacter, Moraxella, and Kingella based on partial 16S ribosomal DNA sequence analysis
Volume: 44
Page(s): 387-91
Year: 1994
Keyword(s): GENBANK/X74893 GENBANK/X74894 GENBANK/X74895 GENBANK/X74896 GENBANK/X74897 GENBANK/X74898 GENBANK/X74899 GENBANK/X74900 GENBANK/X74901 GENBANK/X74902 GENBANK/X74903 Acinetobacter/classification/genetics Base Sequence Comparative Study DNA Primers/genetics DNA, Bacterial/*genetics DNA, Ribosomal/*genetics Kingella/classification/genetics Molecular Sequence Data Moraxella/classification/genetics Neisseria/classification/genetics Neisseriaceae/*classification/*genetics Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Homology, Nucleic Acid Species Specificity Support, Non-U.S. Gov't
Remarks: We obtained 16S ribosomal DNA (rDNA) sequence data for strains belonging to 11 species of Proteobacteria, including the type strains of Kingella kingae, Neisseria lactamica, Neisseria meningitidis, Moraxella lacunata subsp. lacunata, [Neisseria] ovis, Moraxella catarrhalis, Moraxella osloensis, [Moraxella] phenylpyruvica, and Acinetobacter lwoffii, as well as strains of Neisseria subflava and Acinetobacter calcoaceticus. The data in a distance matrix constructed by comparing the sequences supported the proposal that the genera Acinetobacter and Moraxella and [N.] ovis should be excluded from the family Neisseriaceae. Our results are consistent with hybridization data which suggest that these excluded taxa should be part of a new family, the Moraxellaceae. The strains that we studied can be divided into the following five groups: (i) M. lacunata subsp. lacunata, [N.] ovis, and M. catarrhalis; (ii) M. osloensis; (iii) [M.] phenylpyruvica; (iv) A. calcoaceticus and A. lwoffii; and (v) N. meningitidis, N. subflava, N. lactamica, and K. kingae. We agree with the previous proposal that [N.] ovis should be renamed Moraxella ovis, as this organism is closely related to Moraxella species and not to Neisseria species. The generically misnamed taxon [M.] phenylpyruvica belongs to the proposed family Moraxellaceae, but it is sufficiently different to warrant exclusion from the genus Moraxella. Further work needs to be done to investigate genetically similar species, such as Psychrobacter immobilis, before the true generic position of this organism can be determined. Automated 16S rDNA sequencing with the PCR allows workers to accurately determine phylogenetic relationships between groups of organisms.(ABSTRACT TRUNCATED AT 250 WORDS)
URL: 94347600
Data: (ATCC 33078) Type strain / K. Bovre, Oslo, Norway in 1978 / Ovine infectious Keratoconjunctivitis, in 1955 / Neisseria ovis / Bovre, K. (1965) Acta path. microbial. Scand. 64, 229; (1967) 69, 92, 123 / Bovre, K. et al. (1969) Canad. J. Microbiol. 15, 335 / Jantzen, E. et al. (1974) Acta path. microbiol. Scand. sect. B 82, 767 / Bryn, K. et al. (1977) J. gen. Microbiol. 102, 33
Accession Date: 01/01/1978
Authority: (Lindquist 1960) Bovre 1968
Depositor: BOVRE K
Taxonomy: TaxLink: S1917 (Moraxella (branhamella) ovis (lindquist 1960) bovre 1979) - Date of change: 16/06/2007 by NCTCUp to 16/06/2007: ? (NCTC 11227) - Date of change: 04/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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