NCTC Number: |
NCTC 11168
|
Current Name: |
Campylobacter jejuni subsp. jejuni
|
Original Strain Reference: |
5636/77 Lucitt
|
Other Collection No: |
BIOTYPE 1; 5636/77 LUCITT; WDCM 00073
|
Previous Catalogue Name: |
Campylobacter jejuni subsp. jejuni
|
Type Strain: |
No
|
Family: |
Campylobacteraceae
|
Hazard Group (ACDP): |
2
|
Release Restrictions: |
Terms & Conditions of Supply of Microbial Pathogens: Safety
|
Antigenic Properties: |
serovar penner serotype 2
|
Conditions for growth on solid media: |
Columbia blood agar, 48 hours, 37°C, microaerophilic
|
Conditions for growth on liquid media: |
nutrient broth,37, microaerophilic
|
Isolated From: |
human, faeces
|
Whole Genome Sequence: |
http://www.ebi.ac.uk/ena/data/view/ERS980039
|
Annotated Genome: |
ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
|
16S rRNA Gene Sequence: |
>gb|AJ000862|NCTC 11168|Campylobacter jejuni 16S rRNA gene, partial.| acggatctgctggaa...
|
23S rRNA Gene Sequence: |
>gb|AJ000858|NCTC 11168|Campylobacter jejuni 23S rRNA gene, partial.| tgtaagaactagtgg... >gb|X87293|BIOTYPE 1 ATCC 27562 T|V.vulnificus 23S rRNA gene, biotype 1.| ggttaagtgactaag...
|
Miscellaneous Sequence Data: |
>gb|AJ300551|CIP 5731T| ATCC 13337T| BIOTYPE 1|Hafnia alvei partial gyrB gene for DNA gyrase B subunit strain CIP5731T, ATCC 13337T, Biotype 1.| ataagtttgatgaca...
|
Bibliography: |
SKIRROW M B 1977 BR MED J II 9;BUTZLER J P ET AL 1973 J PEDIAT 82 493
|
Extended Bibliography: |
Show bibliography
Ref #: |
32263 |
Author(s): |
Christensen,H.;Jorgensen,K.;Olsen,J.E. |
Journal: |
Microbiology |
Title: |
Differentiation of Campylobacter coli and C. jejuni by length and DNA sequence of the 16S-23S rRNA internal spacer region |
Volume: |
145 ( Pt 1) |
Page(s): |
99-105 |
Year: |
1999 |
Keyword(s): |
GENBANK/AF074828
GENBANK/AF074829
GENBANK/AF074830
GENBANK/AF074831
GENBANK/AF074832
GENBANK/AF074833
GENBANK/AF074834
GENBANK/AF074835
GENBANK/AF074836
GENBANK/AF074837
GENBANK/AF074838
GENBANK/AF074839
GENBANK/AF074840
GENBANK/AF074841
Animals
Bacterial Typing Techniques
Base Sequence
Campylobacter coli/classification/*genetics
Campylobacter jejuni/classification/*genetics
Chickens/microbiology
Conserved Sequence/genetics
DNA, Bacterial/chemistry/genetics
DNA, Ribosomal/chemistry/*genetics
Electrophoresis, Polyacrylamide Gel
Genes, rRNA
Genotype
Humans
Molecular Sequence Data
Molecular Weight
Nucleic Acid Conformation
Polymerase Chain Reaction
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/*genetics
Swine/microbiology
Variation (Genetics)/genetics
|
Remarks: |
The internal spacer region (ISR) between the 16S and 23S rRNA genes of Campylobacter was investigated by PCR fragment length typing and DNA sequencing of clinical and chicken wild-type isolates. PCR fragment length typing showed one fragment of 859 nt in length for the 12 strains of Campylobacter coli investigated. Thirty-six of the Campylobacter jejuni subsp. jejuni strains possessed one fragment, which varied in size between 727 and 802 nt. Three strains showed two fragments between 501 and 923 nt. Strains of C. jejuni subsp. doylei, Campylobacter lari and Campylobacter upsaliensis possessed one or two fragments with lengths different from those of C. coli and C. jejuni subsp. jejuni. DNA sequences were obtained from 54 nt downstream of rrs up to rrl of four strains of C. coli, eight strains of C. jejuni subsp. jejuni, and one strain each of C. jejuni subsp. doylei and C. lari, selected to represent the different biotypes of Campylobacter. ISR lengths determined by PCR fragment length typing and DNA sequencing corresponded for 12 strains. For two strains of C. coli, PCR fragment length typing underestimated ISR lengths by 159 and 193 nt, probably related to incomplete resolution of the distal helical structures, which were not fully denatured during PAGE. For the 14 strains and the published C. jejuni subsp. jejuni sequence, the first 206-211 nt were conserved and included the two tRNA genes in the characteristic tRNA(Ala) to tRNA(Ile) order separated by a short 8-9 nt spacer region. Within the region downstream of tRNA(Ile) conserved regions were identified which allowed a separation of C. lari from C. coli and C. jejuni but not separation of C. coli from C. jejuni. The 69-282 nt longer variable regions in C. coli strains allowed separation of this species from C. jejuni, confirming results obtained by PCR typing. Certain nucleic acid positions in variable regions were related to the Lior biotypes. Sequence information from ISRs of more strains is needed to ascertain if separation of species and biotypes will be possible for diagnostic purposes. |
URL: |
10206715 |
|
Ref #: |
48709 |
Author(s): |
Dauga,C. |
Journal: |
Int J Syst Evol Microbiol |
Title: |
Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies |
Volume: |
52 |
Page(s): |
531-47 |
Year: |
2002 |
Keyword(s): |
GENBANK/AJ300528
GENBANK/AJ300529
GENBANK/AJ300530
GENBANK/AJ300531
GENBANK/AJ300532
GENBANK/AJ300533
GENBANK/AJ300534
GENBANK/AJ300535
GENBANK/AJ300536
GENBANK/AJ300537
GENBANK/AJ300538
GENBANK/AJ300539
GENBANK/AJ300540
GENBANK/AJ300541
GENBANK/AJ300542
GENBANK/AJ300543
GENBANK/AJ300544
GENBANK/AJ300545
GENBANK/AJ300546
GENBANK/AJ300547
GENBANK/AJ300548
GENBANK/AJ300549
GENBANK/AJ300550
GENBANK/AJ300551
GENBANK/AJ300552
GENBANK/AJ300553
GENBANK/AJ300554
DNA Gyrase/*genetics
Enterobacteriaceae/*classification/genetics
Evolution, Molecular
Genes, rRNA
Molecular Sequence Data
Phenotype
RNA, Bacterial/chemistry
RNA, Ribosomal, 16S/chemistry
|
Remarks: |
Phylogenetic trees showing the evolutionary relatedness of Enterobacteriaceae based upon gyrB and 16S rRNA genes were compared. Congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. Phylogenetic trees based on gyrB sequences appeared to be more reliable at determining relationships among Serratia species than trees based on 16S rRNA gene sequences. gyrB sequences from Serratia species formed a monophyletic group validated by significant bootstrap values. Serratia fonticola had the most deeply branching gyrB sequence in the Serratia monophyletic group, which was consistent with its atypical phenotypic characteristics. Klebsiella and Enterobacter genera seemed to be polyphyletic, but the branching patterns of gyrB and 16S rRNA gene trees were not congruent. Enterobacter aerogenes was grouped with Klebsiella pneumoniae on the gyrB phylogenetic tree, which supports that this species could be transferred to the Klebsiella genus. Unfortunately, 16S rRNA and gyrB phylogenetic trees gave conflicting evolutionary relationships for Citrobacter freundii because of its unusual gyrB evolutionary process. gyrB lateral gene transfer was suspected for Hafnia alvei. Saturation of gyrB genes was observed by the pairwise comparison of Proteus spp., Providencia alcalifaciens and Morganella morganii sequences. Depending on their level of variability, 16S rRNA gene sequences were useful for describing phylogenetic relationships between distantly related Enterobacteriaceae, whereas gyrB sequence comparison was useful for inferring intra- and some intergeneric relationships. |
URL: |
11931166 |
|
Ref #: |
12362 |
Author(s): |
Christensen,H.;Jorgensen,K.;Olsen,J.E. |
Journal: |
Microbiology |
Title: |
Differentiation of Campylobacter coli and C. jejuni by length and DNA sequence of the 16S-23S rRNA internal spacer region |
Volume: |
145 ( Pt 1) |
Page(s): |
99-105 |
Year: |
1999 |
Keyword(s): |
GENBANK/AF074828
GENBANK/AF074829
GENBANK/AF074830
GENBANK/AF074831
GENBANK/AF074832
GENBANK/AF074833
GENBANK/AF074834
GENBANK/AF074835
GENBANK/AF074836
GENBANK/AF074837
GENBANK/AF074838
GENBANK/AF074839
GENBANK/AF074840
GENBANK/AF074841
Animal
Bacterial Typing Techniques
Base Sequence
Campylobacter coli/classification/*genetics
Campylobacter jejuni/classification/*genetics
Chickens/microbiology
Comparative Study
Conserved Sequence/genetics
DNA, Bacterial/chemistry/genetics
DNA, Ribosomal/chemistry/*genetics
Electrophoresis, Polyacrylamide Gel
Genes, rRNA
Genotype
Human
Molecular Sequence Data
Molecular Weight
Nucleic Acid Conformation
Polymerase Chain Reaction
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/*genetics
Swine/microbiology
Variation (Genetics)/genetics
|
Remarks: |
The internal spacer region (ISR) between the 16S and 23S rRNA genes of Campylobacter was investigated by PCR fragment length typing and DNA sequencing of clinical and chicken wild-type isolates. PCR fragment length typing showed one fragment of 859 nt in length for the 12 strains of Campylobacter coli investigated. Thirty-six of the Campylobacter jejuni subsp. jejuni strains possessed one fragment, which varied in size between 727 and 802 nt. Three strains showed two fragments between 501 and 923 nt. Strains of C. jejuni subsp. doylei, Campylobacter lari and Campylobacter upsaliensis possessed one or two fragments with lengths different from those of C. coli and C. jejuni subsp. jejuni. DNA sequences were obtained from 54 nt downstream of rrs up to rrl of four strains of C. coli, eight strains of C. jejuni subsp. jejuni, and one strain each of C. jejuni subsp. doylei and C. lari, selected to represent the different biotypes of Campylobacter. ISR lengths determined by PCR fragment length typing and DNA sequencing corresponded for 12 strains. For two strains of C. coli, PCR fragment length typing underestimated ISR lengths by 159 and 193 nt, probably related to incomplete resolution of the distal helical structures, which were not fully denatured during PAGE. For the 14 strains and the published C. jejuni subsp. jejuni sequence, the first 206-211 nt were conserved and included the two tRNA genes in the characteristic tRNA(Ala) to tRNA(Ile) order separated by a short 8-9 nt spacer region. Within the region downstream of tRNA(Ile) conserved regions were identified which allowed a separation of C. lari from C. coli and C. jejuni but not separation of C. coli from C. jejuni. The 69-282 nt longer variable regions in C. coli strains allowed separation of this species from C. jejuni, confirming results obtained by PCR typing. Certain nucleic acid positions in variable regions were related to the Lior biotypes. Sequence information from ISRs of more strains is needed to ascertain if separation of species and biotypes will be possible for diagnostic purposes. |
URL: |
99140141 |
|
|
Data: |
M. B. Skirrow, PHLS Worcester in 1977 / Faeces / Biotype 1, Penner serotype 2 / Butzler, J. P. et al. (1973) J. Pediat. 82, 493 / Skirrow, M. B. (1977) Br. med. J. ii, 9
|
Accession Date: |
01/01/1977
|
History: |
ISOLATED BY SKIRROW M B,WORCESTER PHLS 1977
|
Authority: |
(Jones et al. 1931) Veron and Chatelain 1973 (AL)
|
Depositor: |
SKIRROW M B
|
Taxonomy: |
TaxLink: S675 (Campylobacter jejuni subsp. jejuni (Jones et al. 1931) Veron and Chatelain 1973) - Date of change: 5/02/2003
|
Biosafety Responsibility: |
It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country
|
The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.
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