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Bacteria Collection: Bacteroides ovatus

NCTC Number: NCTC 11153
Current Name: Bacteroides ovatus
Original Strain Reference: 11153
Other Collection No: ATCC 8483; DSM 1896
Previous Catalogue Name: Bacteroides ovatus
Other Names: BACILLUS OVALIS, EGGERTH AND GAGNON 1933
Type Strain: Yes
Family: Bacteroidaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Fastidious anaerobe agar, 24-48 hours, 37°C, anaerobic
Conditions for growth on liquid media: viand-leure broth,37, strict anaerobe
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1295516
16S rRNA Gene Sequence: >gb|AF176691|ATCC8483|Bacteroides ovatus 16S-23S rDNA intergenic spacer region.| ggagcgatgtcgtaa... >gb|X83952|ATCC 8483T|B.ovatus 16S rRNA (ATCC 8483T).| atgaacgctagctac...
23S rRNA Gene Sequence: >gb|AF176691|ATCC8483|Bacteroides ovatus 16S-23S rDNA intergenic spacer region.| ggagcgatgtcgtaa... >gb|AY155589|ATCC 8483|Bacteroides ovatus 23S ribosomal RNA gene, partial sequence.| aagcagccattcatt...
Bibliography: EGGERTH A H & GAGNON B H 1933 J BACT 25 389; MOORE W E C & HOLDEMAN L V
Extended Bibliography: showhide Show bibliography
Ref #: 95538
Author(s): Ruimy,R.;Podglajen,I.;Breuil,J.;Christen,R.;Collatz,E.
Journal: J Bacteriol
Title: A recent fixation of cfiA genes in a monophyletic cluster of Bacteroides fragilis is correlated with the presence of multiple insertion elements
Volume: 178
Page(s): 1914-8
Year: 1996
Keyword(s): GENBANK/X83935 GENBANK/X83936 GENBANK/X83937 GENBANK/X83938 GENBANK/X83939 GENBANK/X83940 GENBANK/X83941 GENBANK/X83942 GENBANK/X83943 GENBANK/X83944 GENBANK/X83945 GENBANK/X83946 GENBANK/X83947 GENBANK/X83948 GENBANK/X83949 GENBANK/X83950 GENBANK/X83951 GENBANK/X83952 GENBANK/X83953 GENBANK/X83954 *Bacterial Proteins Bacteroides fragilis/*classification/*genetics Base Sequence *DNA Transposable Elements DNA, Bacterial DNA, Ribosomal Flavobacterium/classification/genetics Flexibacteraceae/classification/genetics *Genes, Bacterial Molecular Sequence Data Multigene Family Phylogeny beta-Lactamases/classification/*genetics
Remarks: Small-subunit ribosomal DNA sequences of 16 strains of Bacteroides fragilis were determined and compared with previously published sequences. Three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods) as well as a bootstrap analysis were used to assess the robustness of each topology. All phylogenetic analyses demonstrated that the B. fragilis strains were clearly divided into two robust monophyletic units which corresponded to the cfiA-negative and cfiA-positive groups. Strains of two previously identified DNA homology groups separated similarly into the two monophyletic units. According to the intensity of the hybridization signal with a cfiA probe, the cfiA-positive cluster could be further divided into two groups. This difference might reflect the existence of two, probably closely related cfiA-type genes. In the strongly hybridizing cfiA-positive strains, the gene is capable of conferring high-level resistance to the carbapenems and to most beta-lactamase inhibitors as well, while in the weakly hybridizing cfiA-positive strains, only the latter type of resistance is known to occur. The presence of the cfiA-type genes within a monophyletic cluster of B. fragilis that apparently represents only a minority of the species B. fragilis is suggestive of a recent acquisition. The fact that this cluster is also the predominant pool of all known B. fragilis insertion elements, which have been found to play an important role in the expression of carbapenem resistance, raises the possibility that both genetic determinants, i.e., the resistance gene(s) and insertion elements, may have coevolved.
URL: 8606165
Ref #: 25269
Author(s): Liu,C.;Song,Y.;McTeague,M.;Vu,A.W.;Wexler,H.;Finegold,S.M.
Journal: FEMS Microbiol Lett
Title: Rapid identification of the species of the Bacteroides fragilis group by multiplex PCR assays using group- and species-specific primers
Volume: 222
Page(s): 9-16
Year: 2003
Keyword(s): Bacteriological Techniques Bacteroides fragilis/*classification/*genetics/growth & development DNA Transposable Elements/genetics Phenotype Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics
Remarks: We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were first divided into three subgroups by multiplex PCR-G, followed by three multiplex PCR assays with three species-specific primer mixtures for identification to the species level. The primers were designed from nucleotide sequences of the 16S rRNA, the 16S-23S rRNA intergenic spacer region and part of the 23S rRNA gene. The established two-step multiplex PCR identification scheme was applied to the identification of 155 clinical isolates of the B. fragilis group that were previously identified to the species level by phenotypic tests. The new scheme was more accurate than phenotypic identification, which was accurate only 84.5% of the time. The multiplex PCR scheme established in this study is a simple, rapid and reliable method for the identification of the B. fragilis group species. This will permit more accurate assessment of the role of various B. fragilis group members in infections and of the degree of antimicrobial resistance in each of the group members.
URL: 12757940
Ref #: 12216
Author(s): Ruimy,R.;Podglajen,I.;Breuil,J.;Christen,R.;Collatz,E.
Journal: J Bacteriol
Title: A recent fixation of cfiA genes in a monophyletic cluster of Bacteroides fragilis is correlated with the presence of multiple insertion elements
Volume: 178
Page(s): 1914-8
Year: 1996
Keyword(s): GENBANK/X83935 GENBANK/X83936 GENBANK/X83937 GENBANK/X83938 GENBANK/X83939 GENBANK/X83940 GENBANK/X83941 GENBANK/X83942 GENBANK/X83943 GENBANK/X83944 GENBANK/X83945 GENBANK/X83946 GENBANK/X83947 GENBANK/X83948 GENBANK/X83949 GENBANK/X83950 GENBANK/X83951 GENBANK/X83952 GENBANK/X83953 GENBANK/X83954 Bacteroides fragilis/*classification/*genetics Base Sequence Cytophagaceae/classification/genetics *DNA Transposable Elements DNA, Bacterial DNA, Ribosomal Flavobacterium/classification/genetics *Genes, Bacterial Molecular Sequence Data Multigene Family Phylogeny Support, Non-U.S. Gov't beta-Lactamases/classification/*genetics
Remarks: Small-subunit ribosomal DNA sequences of 16 strains of Bacteroides fragilis were determined and compared with previously published sequences. Three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods) as well as a bootstrap analysis were used to assess the robustness of each topology. All phylogenetic analyses demonstrated that the B. fragilis strains were clearly divided into two robust monophyletic units which corresponded to the cfiA-negative and cfiA-positive groups. Strains of two previously identified DNA homology groups separated similarly into the two monophyletic units. According to the intensity of the hybridization signal with a cfiA probe, the cfiA-positive cluster could be further divided into two groups. This difference might reflect the existence of two, probably closely related cfiA-type genes. In the strongly hybridizing cfiA-positive strains, the gene is capable of conferring high-level resistance to the carbapenems and to most beta-lactamase inhibitors as well, while in the weakly hybridizing cfiA-positive strains, only the latter type of resistance is known to occur. The presence of the cfiA-type genes within a monophyletic cluster of B. fragilis that apparently represents only a minority of the species B. fragilis is suggestive of a recent acquisition. The fact that this cluster is also the predominant pool of all known B. fragilis insertion elements, which have been found to play an important role in the expression of carbapenem resistance, raises the possibility that both genetic determinants, i.e., the resistance gene(s) and insertion elements, may have coevolved.
URL: 96178954
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 1394
Author(s): Cato,E.P.;Johnson,J.L.
Journal: Int. J. Syst. Bacteriol.
Title: Reinstatement of species rank for Bacteroides fragilis, B. ovatus, B. distasonis, B. thetajotaomicron, and B. vulgatus: designation of neotype strains for Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers and Bacteroides thetajotaomicron (
Volume: 26
Page(s): 230-237
Year: 1976
Ref #: 3968
Author(s): Johnson,J.L.
Journal: Int. J. Syst. Bacteriol.
Title: Taxonomy of the bacteroides. I. Deoxyribonucleic acid homologies among Bacteroides fragilis and other saccharolytic Bacteroides species.
Volume: 28
Page(s): 245-256
Year: 1978
Data: (ATCC 8483, DSM 1869) Type strain / ATCC in 1977 / A. H. Eggerth in 1942 (Redeposited in 1954 and 1956) / Eggerth, A. H. & Gagnon, B. H. (1933) J. Bact. 25, 389 / Moore, W. E. C. & Holdeman, L. V. (1973) Int. J. syst. Bact. 23, 69 / Cato, E. P. & Johnson, J. L. (1976) Int. J. syst. Bact. 26, 230
Accession Date: 01/01/1977
History: ISOLATED BY EGGERTH A H,1942;REPLACEMENT 1942 & 1956
Authority: Eggerth and Gagnon 1933 (AL)
Depositor: ATCC
Taxonomy: TaxLink: S487 (Bacteroides ovatus Eggerth and Gagnon 1933) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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