NCTC Number: |
NCTC 11152
|
Current Name: |
Bacteroides distasonis
|
Original Strain Reference: |
ATCC 8503
|
Other Collection No: |
ATCC 8503; DSM 20701
|
Previous Catalogue Name: |
Bacteroides distasonis
|
Type Strain: |
Yes
|
Family: |
Bacteroidaceae
|
Hazard Group (ACDP): |
2
|
Release Restrictions: |
Terms & Conditions of Supply of Microbial Pathogens: Safety
|
Conditions for growth on solid media: |
Blood agar (Fastidious anaerobe agar), 48 hours, 37°C, anaerobic
|
Conditions for growth on liquid media: |
viande-leure broth,37, facultative anaerobe
|
Whole Genome Sequence: |
http://www.ebi.ac.uk/ena/data/view/ERS1324154
|
16S rRNA Gene Sequence: |
>gb|AF176689|ATCC8503|Bacteroides distasonis 16S-23S rDNA intergenic spacer region.| ggagcttcaggattc... >gb|AY546490|ATCC 8503|Bacteroides distasonis strain ATCC 8503 16S-23S ribosomal RNAintergenic spacer, partial sequence.| cgcgaggatcggcct...
|
23S rRNA Gene Sequence: |
>gb|AF176689|ATCC8503|Bacteroides distasonis 16S-23S rDNA intergenic spacer region.| ggagcttcaggattc... >gb|AY546490|ATCC 8503|Bacteroides distasonis strain ATCC 8503 16S-23S ribosomal RNAintergenic spacer, partial sequence.| cgcgaggatcggcct... >gb|AY155594|ATCC 8503|Bacteroides distasonis 23S ribosomal RNA gene, partial sequence.| aagcagccattcatt...
|
Bibliography: |
CATO E P & JONSON J L 1976 INT J SYST BACT 26 230; MOORE W E C & HOLDEMAN L V 1973 INT J SYST BACT 23 69
|
Extended Bibliography: |
Show bibliography
Ref #: |
25269 |
Author(s): |
Liu,C.;Song,Y.;McTeague,M.;Vu,A.W.;Wexler,H.;Finegold,S.M. |
Journal: |
FEMS Microbiol Lett |
Title: |
Rapid identification of the species of the Bacteroides fragilis group by multiplex PCR assays using group- and species-specific primers |
Volume: |
222 |
Page(s): |
9-16 |
Year: |
2003 |
Keyword(s): |
Bacteriological Techniques
Bacteroides fragilis/*classification/*genetics/growth & development
DNA Transposable Elements/genetics
Phenotype
Polymerase Chain Reaction/*methods
RNA, Ribosomal, 16S/genetics
RNA, Ribosomal, 23S/genetics
|
Remarks: |
We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were first divided into three subgroups by multiplex PCR-G, followed by three multiplex PCR assays with three species-specific primer mixtures for identification to the species level. The primers were designed from nucleotide sequences of the 16S rRNA, the 16S-23S rRNA intergenic spacer region and part of the 23S rRNA gene. The established two-step multiplex PCR identification scheme was applied to the identification of 155 clinical isolates of the B. fragilis group that were previously identified to the species level by phenotypic tests. The new scheme was more accurate than phenotypic identification, which was accurate only 84.5% of the time. The multiplex PCR scheme established in this study is a simple, rapid and reliable method for the identification of the B. fragilis group species. This will permit more accurate assessment of the role of various B. fragilis group members in infections and of the degree of antimicrobial resistance in each of the group members. |
URL: |
12757940 |
|
Ref #: |
25447 |
Author(s): |
Conrads,G.;Citron,D.M.;Tyrrell,K.L.;Horz,H.P.;Goldstein,E.J. |
Journal: |
Int J Syst Evol Microbiol |
Title: |
16S-23S rRNA gene internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus Porphyromonas |
Volume: |
55 |
Page(s): |
607-13 |
Year: |
2005 |
Keyword(s): |
GENBANK/AB034799
GENBANK/AB035460
GENBANK/AF208290
GENBANK/AY546472
GENBANK/AY546473
GENBANK/AY546474
GENBANK/AY546475
GENBANK/AY546476
GENBANK/AY546477
GENBANK/AY546478
GENBANK/AY546479
GENBANK/AY546480
GENBANK/AY546481
GENBANK/AY546482
GENBANK/AY546483
GENBANK/AY546484
GENBANK/AY546485
GENBANK/AY546486
GENBANK/AY546487
GENBANK/AY546488
GENBANK/AY546489
GENBANK/AY546490
Bacterial Typing Techniques
DNA, Ribosomal Spacer/*analysis
Genes, rRNA
Humans
Molecular Sequence Data
*Phylogeny
Porphyromonas/*classification/genetics
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/*genetics
Sequence Analysis, DNA
Species Specificity
|
Remarks: |
The 16S-23S rRNA gene internal transcribed spacer (ITS) regions of 11 reference strains of Porphyromonas species, together with Bacteroides distasonis and Tannerella forsythensis, were analysed to examine interspecies relationships. Compared with the phylogenetic tree generated using 16S rRNA gene sequences, the resolution of the ITS sequence-based tree was higher, but species positioning and clustering were similar with both approaches. The recent separation of Porphyromonas gulae and Porphyromonas gingivalis into distinct species was confirmed by the ITS data. In addition, analysis of the ITS sequences of 24 clinical isolates of Porphyromonas asaccharolytica plus the type strain ATCC 25260(T) divided the sequences into two clusters, of which one was alpha-fucosidase-positive (like the type strain) while the other was alpha-fucosidase-negative. The latter resembled the previously studied unusual extra-oral isolates of 'Porphyromonas endodontalis-like organisms' (PELOs) which could therefore be called 'Porphyromonas asaccharolytica-like organisms' (PALOs), based on the genetic identification. Moreover, the proposal of alpha-fucosidase-negative P. asaccharolytica strains as a new species should also be considered. |
URL: |
15774632 |
|
Ref #: |
1300 |
Author(s): |
Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed) |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Approved Lists of Bacterial Names. |
Volume: |
30 |
Page(s): |
225-420 |
Year: |
1980 |
|
Ref #: |
3968 |
Author(s): |
Johnson,J.L. |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Taxonomy of the bacteroides. I. Deoxyribonucleic acid homologies among Bacteroides fragilis and other saccharolytic Bacteroides species. |
Volume: |
28 |
Page(s): |
245-256 |
Year: |
1978 |
|
|
Data: |
Type strain / ATCC in 1977 / A. H. Eggerth in 1942 / Eggerth, A. H. & Gagnon, B. H. (1933) J. Bact. 25, 389 / Moore, W. E. C. & Holdeman, L. V. (1973) Int. J. syst. Bact. 23, 69 / Cato, E. P. & Johnson, J. L. (1976) Int. J. syst. Bact. 26, 230
|
Accession Date: |
01/01/1977
|
History: |
EGGERTH A H IN 1942
|
Authority: |
Eggerth and Gagnon 1933 (AL)
|
Depositor: |
ATCC
|
Taxonomy: |
TaxLink: S460 (Bacteroides distasonis Eggerth and Gagnon 1933) - Date of change: 5/02/2003
|
Biosafety Responsibility: |
It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country
|
The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.
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