Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Bacteroides distasonis

NCTC Number: NCTC 11152
Current Name: Bacteroides distasonis
Original Strain Reference: ATCC 8503
Other Collection No: ATCC 8503; DSM 20701
Previous Catalogue Name: Bacteroides distasonis
Type Strain: Yes
Family: Bacteroidaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Blood agar (Fastidious anaerobe agar), 48 hours, 37°C, anaerobic
Conditions for growth on liquid media: viande-leure broth,37, facultative anaerobe
Whole Genome Sequence:
16S rRNA Gene Sequence: >gb|AF176689|ATCC8503|Bacteroides distasonis 16S-23S rDNA intergenic spacer region.| ggagcttcaggattc... >gb|AY546490|ATCC 8503|Bacteroides distasonis strain ATCC 8503 16S-23S ribosomal RNAintergenic spacer, partial sequence.| cgcgaggatcggcct...
23S rRNA Gene Sequence: >gb|AF176689|ATCC8503|Bacteroides distasonis 16S-23S rDNA intergenic spacer region.| ggagcttcaggattc... >gb|AY546490|ATCC 8503|Bacteroides distasonis strain ATCC 8503 16S-23S ribosomal RNAintergenic spacer, partial sequence.| cgcgaggatcggcct... >gb|AY155594|ATCC 8503|Bacteroides distasonis 23S ribosomal RNA gene, partial sequence.| aagcagccattcatt...
Bibliography: CATO E P & JONSON J L 1976 INT J SYST BACT 26 230; MOORE W E C & HOLDEMAN L V 1973 INT J SYST BACT 23 69
Extended Bibliography: showhide Show bibliography
Ref #: 25269
Author(s): Liu,C.;Song,Y.;McTeague,M.;Vu,A.W.;Wexler,H.;Finegold,S.M.
Journal: FEMS Microbiol Lett
Title: Rapid identification of the species of the Bacteroides fragilis group by multiplex PCR assays using group- and species-specific primers
Volume: 222
Page(s): 9-16
Year: 2003
Keyword(s): Bacteriological Techniques Bacteroides fragilis/*classification/*genetics/growth & development DNA Transposable Elements/genetics Phenotype Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics
Remarks: We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were first divided into three subgroups by multiplex PCR-G, followed by three multiplex PCR assays with three species-specific primer mixtures for identification to the species level. The primers were designed from nucleotide sequences of the 16S rRNA, the 16S-23S rRNA intergenic spacer region and part of the 23S rRNA gene. The established two-step multiplex PCR identification scheme was applied to the identification of 155 clinical isolates of the B. fragilis group that were previously identified to the species level by phenotypic tests. The new scheme was more accurate than phenotypic identification, which was accurate only 84.5% of the time. The multiplex PCR scheme established in this study is a simple, rapid and reliable method for the identification of the B. fragilis group species. This will permit more accurate assessment of the role of various B. fragilis group members in infections and of the degree of antimicrobial resistance in each of the group members.
URL: 12757940
Ref #: 25447
Author(s): Conrads,G.;Citron,D.M.;Tyrrell,K.L.;Horz,H.P.;Goldstein,E.J.
Journal: Int J Syst Evol Microbiol
Title: 16S-23S rRNA gene internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus Porphyromonas
Volume: 55
Page(s): 607-13
Year: 2005
Keyword(s): GENBANK/AB034799 GENBANK/AB035460 GENBANK/AF208290 GENBANK/AY546472 GENBANK/AY546473 GENBANK/AY546474 GENBANK/AY546475 GENBANK/AY546476 GENBANK/AY546477 GENBANK/AY546478 GENBANK/AY546479 GENBANK/AY546480 GENBANK/AY546481 GENBANK/AY546482 GENBANK/AY546483 GENBANK/AY546484 GENBANK/AY546485 GENBANK/AY546486 GENBANK/AY546487 GENBANK/AY546488 GENBANK/AY546489 GENBANK/AY546490 Bacterial Typing Techniques DNA, Ribosomal Spacer/*analysis Genes, rRNA Humans Molecular Sequence Data *Phylogeny Porphyromonas/*classification/genetics RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Species Specificity
Remarks: The 16S-23S rRNA gene internal transcribed spacer (ITS) regions of 11 reference strains of Porphyromonas species, together with Bacteroides distasonis and Tannerella forsythensis, were analysed to examine interspecies relationships. Compared with the phylogenetic tree generated using 16S rRNA gene sequences, the resolution of the ITS sequence-based tree was higher, but species positioning and clustering were similar with both approaches. The recent separation of Porphyromonas gulae and Porphyromonas gingivalis into distinct species was confirmed by the ITS data. In addition, analysis of the ITS sequences of 24 clinical isolates of Porphyromonas asaccharolytica plus the type strain ATCC 25260(T) divided the sequences into two clusters, of which one was alpha-fucosidase-positive (like the type strain) while the other was alpha-fucosidase-negative. The latter resembled the previously studied unusual extra-oral isolates of 'Porphyromonas endodontalis-like organisms' (PELOs) which could therefore be called 'Porphyromonas asaccharolytica-like organisms' (PALOs), based on the genetic identification. Moreover, the proposal of alpha-fucosidase-negative P. asaccharolytica strains as a new species should also be considered.
URL: 15774632
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 3968
Author(s): Johnson,J.L.
Journal: Int. J. Syst. Bacteriol.
Title: Taxonomy of the bacteroides. I. Deoxyribonucleic acid homologies among Bacteroides fragilis and other saccharolytic Bacteroides species.
Volume: 28
Page(s): 245-256
Year: 1978
Data: Type strain / ATCC in 1977 / A. H. Eggerth in 1942 / Eggerth, A. H. & Gagnon, B. H. (1933) J. Bact. 25, 389 / Moore, W. E. C. & Holdeman, L. V. (1973) Int. J. syst. Bact. 23, 69 / Cato, E. P. & Johnson, J. L. (1976) Int. J. syst. Bact. 26, 230
Accession Date: 01/01/1977
History: EGGERTH A H IN 1942
Authority: Eggerth and Gagnon 1933 (AL)
Depositor: ATCC
Taxonomy: TaxLink: S460 (Bacteroides distasonis Eggerth and Gagnon 1933) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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