Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Staphylococcus intermedius

NCTC Number: NCTC 11048
Current Name: Staphylococcus intermedius
Original Strain Reference: H11/68
Other Collection No: ATCC 29663; CCM5739; CNCTC M16/75; DSM 20373; H11/68
Previous Catalogue Name: Staphylococcus intermedius
Type Strain: Yes
Family: Staphylococcaceae
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient agar,nutrient broth,37, facultative anaerobe
Isolated From: bird;nares,pigeon in 1968
Whole Genome Sequence:
16S rRNA Gene Sequence: >gb|Z26897|NCTC 11048|S.intermedius gene for 16S ribosomal RNA.| ctcaggatgaacgct... >gb|D83369|MAFF 911388| ATCC 29663T|Staphylococcus intermedius (strain MAFF 911388, ATCC 29663T) genefor 16S rRNA, partial sequence.| aggatgaacgctggc... >gb|AY688070|DSM 20373|Staphylococcus intermedius strain DSM 20373 16S ribosomal RNA gene,partial sequence.| catgcaagtcgagcg...
23S rRNA Gene Sequence: >gb|AJ549078|TYPE STRAIN: DSM 20373T|Staphylococcus intermedius 23S rRNA gene, type strain DSM 20373T.| ggagtgcgtagtagc...
Bibliography: HAJEK V 1976 INT.J.SYST.BACT. 26 401 408; HAJEK V & MARSALEK E 1976
Extended Bibliography: showhide Show bibliography
Ref #: 95479
Author(s): Mitterer,G.;Huber,M.;Leidinger,E.;Kirisits,C.;Lubitz,W.;Mueller,M.W.;Schmidt,W.M.
Journal: J Clin Microbiol
Title: Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences
Volume: 42
Page(s): 1048-57
Year: 2004
Keyword(s): Abortion, Veterinary/microbiology Animals Bacteria/*genetics/*isolation & purification Base Sequence DNA Primers DNA, Bacterial/genetics/isolation & purification DNA, Ribosomal/*genetics/isolation & purification Female Horses *Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction/*methods Pregnancy Pregnancy Complications, Infectious/microbiology/veterinary RNA, Bacterial/genetics/isolation & purification RNA, Ribosomal, 23S/*genetics/isolation & purification
Remarks: The rapid identification of the bacteria in clinical samples is important for patient management and antimicrobial therapy. We describe a DNA microarray-based PCR approach for the quick detection and identification of bacteria from cervical swab specimens from mares. This on-chip PCR method combines the amplification of a variable region of bacterial 23S ribosomal DNA and the simultaneous sequence-specific detection on a solid phase. The solid phase contains bacterial species-specific primers covalently bound to a glass support. During the solid-phase amplification reaction the polymerase elongates perfectly matched primers and incorporates biotin-labeled nucleotides. The reaction products are visualized by streptavidin-cyanine 5 staining, followed by fluorescence scanning. This procedure successfully identified from pure cultures 22 bacteria that are common causes of abortion and sterility in mares. Using the on-chip PCR method, we also tested 21 cervical swab specimens from mares for the presence of pathogenic bacteria and compared the results with those of conventional bacteriological culture methods. Our method correctly identified the bacteria in 12 cervical swab samples, 8 of which contained more than one bacterial species. Due to the higher sensitivity of the on-chip PCR, this method identified bacteria in five cervical swab samples which were not detected by the conventional identification procedure. Our results show that this method will have great potential to be incorporated into the routine microbiology laboratory.
URL: 15004052
Ref #: 81199
Author(s): Becker,K.;Harmsen,D.;Mellmann,A.;Meier,C.;Schumann,P.;Peters,G.;von Eiff,C.
Journal: J Clin Microbiol
Title: Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species
Volume: 42
Page(s): 4988-95
Year: 2004
Keyword(s): GENBANK/AY688029 GENBANK/AY688030 GENBANK/AY688031 GENBANK/AY688032 GENBANK/AY688033 GENBANK/AY688034 GENBANK/AY688035 GENBANK/AY688036 GENBANK/AY688037 GENBANK/AY688038 GENBANK/AY688039 GENBANK/AY688040 GENBANK/AY688041 GENBANK/AY688042 GENBANK/AY688043 GENBANK/AY688044 GENBANK/AY688045 GENBANK/AY688046 GENBANK/AY688047 GENBANK/AY688048 GENBANK/AY688049 GENBANK/AY688050 GENBANK/AY688051 GENBANK/AY688052 GENBANK/AY688053 GENBANK/AY688054 GENBANK/AY688055 GENBANK/AY688056 GENBANK/AY688057 GENBANK/AY688058 GENBANK/AY688059 GENBANK/AY688060 GENBANK/AY688061 GENBANK/AY688062 GENBANK/AY688063 GENBANK/AY688064 GENBANK/AY688065 GENBANK/AY688066 GENBANK/AY688067 GENBANK/AY688068 GENBANK/AY688069 GENBANK/AY688070 GENBANK/AY688071 GENBANK/AY688072 GENBANK/AY688073 GENBANK/AY688074 GENBANK/AY688075 GENBANK/AY688076 GENBANK/AY688077 GENBANK/AY688078 GENBANK/AY688079 GENBANK/AY688080 GENBANK/AY688081 GENBANK/AY688082 GENBANK/AY688083 GENBANK/AY688084 GENBANK/AY688085 GENBANK/AY688086 GENBANK/AY688087 GENBANK/AY688088 GENBANK/AY688089 GENBANK/AY688090 GENBANK/AY688091 GENBANK/AY688092 GENBANK/AY688093 GENBANK/AY688094 GENBANK/AY688095 GENBANK/AY688096 GENBANK/AY688097 GENBANK/AY688098 GENBANK/AY688099 GENBANK/AY688100 GENBANK/AY688101 GENBANK/AY688102 GENBANK/AY688103 GENBANK/AY688104 GENBANK/AY688105 GENBANK/AY688106 GENBANK/AY688107 GENBANK/AY688108 GENBANK/AY688109 *Bacterial Typing Techniques DNA, Bacterial/analysis DNA, Ribosomal/analysis *Databases, Nucleic Acid Genes, rRNA Humans Molecular Sequence Data RNA, Ribosomal, 16S/*genetics Sequence Analysis, DNA Species Specificity Staphylococcal Infections/*microbiology Staphylococcus/*classification/genetics/metabolism
Remarks: To establish an improved ribosomal gene sequence database as part of the Ribosomal Differentiation of Microorganisms (RIDOM) project and to overcome the drawbacks of phenotypic identification systems and publicly accessible sequence databases, both strands of the 5' end of the 16S ribosomal DNA (rDNA) of 81 type and reference strains comprising all validly described staphylococcal (sub)species were sequenced. Assuming a normal distribution for pairwise distances of all unique staphylococcal sequences and choosing a reporting criterion of > or =98.7% similarity for a "distinct species," a statistical error probability of 1.0% was calculated. To evaluate this database, a 16S rDNA fragment (corresponding to Escherichia coli positions 54 to 510) of 55 clinical Staphylococcus isolates (including those of the small-colony variant phenotype) were sequenced and analyzed by the RIDOM approach. Of these isolates, 54 (98.2%) had a similarity score above the proposed threshold using RIDOM; 48 (87.3%) of the sequences gave a perfect match, whereas 83.6% were found by searching National Center for Biotechnology Information (NCBI) database entries. In contrast to RIDOM, which showed four ambiguities at the species level (mainly concerning Staphylococcus intermedius versus Staphylococcus delphini), the NCBI database search yielded 18 taxon-related ambiguities and showed numerous matches exhibiting redundant or unspecified entries. Comparing molecular results with those of biochemical procedures, ID 32 Staph (bioMerieux, Marcy I'Etoile, France) and VITEK 2 (bioMerieux) failed to identify 13 (23.6%) and 19 (34.5%) isolates, respectively, due to incorrect identification and/or categorization below acceptable values. In contrast to phenotypic methods and the NCBI database, the novel high-quality RIDOM sequence database provides excellent identification of staphylococci, including rarely isolated species and phenotypic variants.
URL: 15528685
Ref #: 1275
Author(s): Hajek,V.
Journal: Int. J. Syst. Bacteriol.
Title: Staphylococcus intermedius, a new species isolated from animals.
Volume: 26
Page(s): 401-408
Year: 1976
Ref #: 1276
Author(s): Hajek,J.H.;Marsalek,E.
Journal: Zentralbl. Bakteriol. Hyg. I Abt. Orig. A
Title: The differentiation of pathogenic staphylococci and a suggestion for their taxonomic classification.
Volume: 217
Page(s): 176-182
Year: 1972
Ref #: 1277
Author(s): Meyer,S.A.;Schleifer,K.H.
Journal: Arch. Microbiol.
Title: Deoxyribonucleic acid reassociation in the classification of coagulase-positive staphylococci.
Volume: 117
Page(s): 183-188
Year: 1978
Ref #: 1278
Author(s): Schleifer,K.H.;Schumacher-Perdreau,F.;Götz,F.;Popp,B.
Journal: Arch. Microbiol.
Title: Chemical and biochemical studies for the differentiation of coagulase-positive staphylococci.
Volume: 110
Page(s): 263-270
Year: 1976
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 1332
Author(s): Kilpper-Bälz,R.;Schleifer,K.H.
Journal: FEMS Microbiol. Lett.
Title: DNA-rRNA hybridization studies among staphylococci and some other Gram-positive bacteria.
Volume: 10
Page(s): 357-362
Year: 1981
Data: (ATCC 29663) Type strain / V. Hajek, Olomouc, Czechoslovakia in 1976 / Nares, pigeon / Hajek, V. & Marsalek, E. (1976) Zbl. Bakt. Hyg. Orig. 161, 445-461 / Hajek, V. (1976) Int. J. syst. Bact. 26, 401
Accession Date: 01/01/1976
Authority: Hajek 1976 (AL)
Depositor: HAJEK V
Taxonomy: TaxLink: S2791 (Staphylococcus intermedius Hajek 1976) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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