| NCTC Number: |
NCTC 11044
|
| Current Name: |
Staphylococcus warneri
|
| Original Strain Reference: |
CCM 2730
|
| Other Collection No: |
ATCC 27836; AW 25; CCM 2730; DSM 20316
|
| Previous Catalogue Name: |
Staphylococcus warneri
|
| Type Strain: |
Yes
|
| Family: |
Staphylococcaceae
|
| Hazard Group (ACDP): |
2
|
| Release Restrictions: |
Terms & Conditions of Supply of Microbial Pathogens: Safety
|
| Conditions for growth on solid media: |
Nutrient agar, 24-48 hours, 37°C, aerobic
|
| Conditions for growth on liquid media: |
nutrient broth,37, facultative anaerobe
|
| Isolated From: |
human, human skin
|
| Whole Genome Sequence: |
http://www.ebi.ac.uk/ena/data/view/ERS846855
|
| Annotated Genome: |
ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/d...
|
| 16S rRNA Gene Sequence: |
>gb|Z26903|ATCC 27836|S.warneri gene for 16S ribosomal RNA.| ctcaggatgaacgct... >gb|AY688106|DSM 20316|Staphylococcus warneri strain DSM 20316 16S ribosomal RNA gene,partial sequence.| catgcaagtcgagcg...
|
| Bibliography: |
KLOOS W E & SCHLEIFER K H 1975 INT.J.SYST.BACT. 25 62
|
| Extended Bibliography: |
Show bibliography
| Ref #: |
80948 |
| Author(s): |
Drancourt,M.;Raoult,D. |
| Journal: |
J Clin Microbiol |
| Title: |
rpoB gene sequence-based identification of Staphylococcus species |
| Volume: |
40 |
| Page(s): |
1333-8 |
| Year: |
2002 |
| Keyword(s): |
*Bacterial Typing Techniques
DNA-Directed RNA Polymerases/*genetics
Databases, Factual
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
*Sequence Analysis, DNA
Staphylococcus/*classification/genetics
|
| Remarks: |
The complete sequence of rpoB, the gene encoding the beta subunit of RNA polymerase was determined for Staphylococcus saccharolyticus, Staphylococcus lugdunensis, S taphylococcus caprae, and Staphylococcus intermedius and partial sequences were obtained for an additional 27 Staphylococcus species. The complete rpoB sequences varied in length from 3,452 to 3,845 bp and had a 36.8 to 39.2% GC content. The partial sequences had 71.6 to 93.6% interspecies homology and exhibited a 0.08 to 0.8% intraspecific divergence. With a few exceptions, the phylogenetic relationships inferred from the partial rpoB sequences were in agreement with those previously derived from DNA-DNA hybridization studies and analyses of 16S ribosomal DNA gene sequences and partial HSP60 gene sequences. The staphylococcal rpoB sequence database we established enabled us to develop a molecular method for identifying Staphylococcus isolates by PCR followed by direct sequencing of the 751-bp amplicon. In blind tests, this method correctly identified 10 Staphylococcus isolates, and no positive results were obtained with 10 non-Staphylococcus gram-positive and gram-negative bacterial isolates. We propose partial sequencing of the rpoB gene as a new tool for the accurate identification of Staphylococcus isolates. |
| URL: |
11923353 |
|
| Ref #: |
81199 |
| Author(s): |
Becker,K.;Harmsen,D.;Mellmann,A.;Meier,C.;Schumann,P.;Peters,G.;von Eiff,C. |
| Journal: |
J Clin Microbiol |
| Title: |
Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species |
| Volume: |
42 |
| Page(s): |
4988-95 |
| Year: |
2004 |
| Keyword(s): |
GENBANK/AY688029
GENBANK/AY688030
GENBANK/AY688031
GENBANK/AY688032
GENBANK/AY688033
GENBANK/AY688034
GENBANK/AY688035
GENBANK/AY688036
GENBANK/AY688037
GENBANK/AY688038
GENBANK/AY688039
GENBANK/AY688040
GENBANK/AY688041
GENBANK/AY688042
GENBANK/AY688043
GENBANK/AY688044
GENBANK/AY688045
GENBANK/AY688046
GENBANK/AY688047
GENBANK/AY688048
GENBANK/AY688049
GENBANK/AY688050
GENBANK/AY688051
GENBANK/AY688052
GENBANK/AY688053
GENBANK/AY688054
GENBANK/AY688055
GENBANK/AY688056
GENBANK/AY688057
GENBANK/AY688058
GENBANK/AY688059
GENBANK/AY688060
GENBANK/AY688061
GENBANK/AY688062
GENBANK/AY688063
GENBANK/AY688064
GENBANK/AY688065
GENBANK/AY688066
GENBANK/AY688067
GENBANK/AY688068
GENBANK/AY688069
GENBANK/AY688070
GENBANK/AY688071
GENBANK/AY688072
GENBANK/AY688073
GENBANK/AY688074
GENBANK/AY688075
GENBANK/AY688076
GENBANK/AY688077
GENBANK/AY688078
GENBANK/AY688079
GENBANK/AY688080
GENBANK/AY688081
GENBANK/AY688082
GENBANK/AY688083
GENBANK/AY688084
GENBANK/AY688085
GENBANK/AY688086
GENBANK/AY688087
GENBANK/AY688088
GENBANK/AY688089
GENBANK/AY688090
GENBANK/AY688091
GENBANK/AY688092
GENBANK/AY688093
GENBANK/AY688094
GENBANK/AY688095
GENBANK/AY688096
GENBANK/AY688097
GENBANK/AY688098
GENBANK/AY688099
GENBANK/AY688100
GENBANK/AY688101
GENBANK/AY688102
GENBANK/AY688103
GENBANK/AY688104
GENBANK/AY688105
GENBANK/AY688106
GENBANK/AY688107
GENBANK/AY688108
GENBANK/AY688109
*Bacterial Typing Techniques
DNA, Bacterial/analysis
DNA, Ribosomal/analysis
*Databases, Nucleic Acid
Genes, rRNA
Humans
Molecular Sequence Data
RNA, Ribosomal, 16S/*genetics
Sequence Analysis, DNA
Species Specificity
Staphylococcal Infections/*microbiology
Staphylococcus/*classification/genetics/metabolism
|
| Remarks: |
To establish an improved ribosomal gene sequence database as part of the Ribosomal Differentiation of Microorganisms (RIDOM) project and to overcome the drawbacks of phenotypic identification systems and publicly accessible sequence databases, both strands of the 5' end of the 16S ribosomal DNA (rDNA) of 81 type and reference strains comprising all validly described staphylococcal (sub)species were sequenced. Assuming a normal distribution for pairwise distances of all unique staphylococcal sequences and choosing a reporting criterion of > or =98.7% similarity for a "distinct species," a statistical error probability of 1.0% was calculated. To evaluate this database, a 16S rDNA fragment (corresponding to Escherichia coli positions 54 to 510) of 55 clinical Staphylococcus isolates (including those of the small-colony variant phenotype) were sequenced and analyzed by the RIDOM approach. Of these isolates, 54 (98.2%) had a similarity score above the proposed threshold using RIDOM; 48 (87.3%) of the sequences gave a perfect match, whereas 83.6% were found by searching National Center for Biotechnology Information (NCBI) database entries. In contrast to RIDOM, which showed four ambiguities at the species level (mainly concerning Staphylococcus intermedius versus Staphylococcus delphini), the NCBI database search yielded 18 taxon-related ambiguities and showed numerous matches exhibiting redundant or unspecified entries. Comparing molecular results with those of biochemical procedures, ID 32 Staph (bioMerieux, Marcy I'Etoile, France) and VITEK 2 (bioMerieux) failed to identify 13 (23.6%) and 19 (34.5%) isolates, respectively, due to incorrect identification and/or categorization below acceptable values. In contrast to phenotypic methods and the NCBI database, the novel high-quality RIDOM sequence database provides excellent identification of staphylococci, including rarely isolated species and phenotypic variants. |
| URL: |
15528685 |
|
|
| Data: |
(AW 25, ATCC 27836, DSM 20316) Type strain / CCM, Czechoslovakia in 1976 / Human skin / Kloos, W. E. & Schleifer, K. H. (1975) Int. J. syst. Bact. 25, 62
|
| Accession Date: |
01/01/1976
|
| History: |
KLOOS W E ,1975 ;CCM ,CZECHOSLOVAKIA ,1976
|
| Authority: |
Kloos and Schleifer 1975 (AL)
|
| Depositor: |
CCM
|
| Taxonomy: |
TaxLink: S2801 (Staphylococcus warneri Kloos and Schleifer 1975) - Date of change: 5/02/2003
|
| Biosafety Responsibility: |
It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country
|
The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.
Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.
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