Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Staphylococcus xylosus

NCTC Number:

NCTC 11043

Current Name:

Staphylococcus xylosus

Original Strain Reference:

CCM 2738

Other Collection No:

ATCC 29971; CCM 2738; DSM 20266; KL 162

Previous Catalogue Name:

Staphylococcus xylosus

Type Strain:

Yes

Family:

Staphylococcaceae

Hazard Group (ACDP):

Release Restrictions:

Terms & Conditions of Supply of Microbial Pathogens: Safety

Conditions for growth on solid media:

Nutrient agar, 24-48 hours, 37°C, aerobic

Conditions for growth on liquid media:

nutrient broth,37, facultative anaerobe

Isolated From:

human, human skin

Whole Genome Sequence:

http://www.ebi.ac.uk/ena/data/view/ERS846854

16S rRNA Gene Sequence:

>gb|AY126246|DSM20266T|Staphylococcus xylosus strain DSM20266T 16S ribosomal RNA gene,partial sequence.| ttaggagtttgatcc... >gb|AF041357|DSM 20266T|Staphylococcus xylosus 16S ribosomal RNA gene, partial sequence.| ccggaattattgggc... >gb|Z26900|DSM 20266|S.xylosus gene for 16S ribosomal RNA.| ctcaggatgaacgct... >gb|AY688107|DSM 20266|Staphylococcus xylosus strain DSM 20266 16S ribosomal RNA gene,partial sequence.| catgcaagtcgagcg...

Bibliography:

SCHEIFER K H & KLOOS W E 1975 INT.J.SYST.BACT. 25 50

Extended Bibliography:

Show bibliography

Ref #:	55499
Author(s):	Blaiotta,G.;Pennacchia,C.;Ercolini,D.;Moschetti,G.;Villani,F.
Journal:	Syst Appl Microbiol
Title:	Combining denaturing gradient gel electrophoresis of 16S rDNA V3 region and 16S-23S rDNA spacer region polymorphism analyses for the identification of staphylococci from Italian fermented sausages
Volume:	26
Page(s):	423-33
Year:	2003
Keyword(s):	DNA, Bacterial/isolation & purification DNA, Ribosomal/analysis DNA, Ribosomal Spacer/analysis Electrophoresis, Agar Gel Electrophoresis, Polyacrylamide Gel Food Microbiology Gram-Positive Cocci/classification/genetics/isolation & purification Meat Products/microbiology Micrococcus Molecular Sequence Data Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Staphylococcus/*classification/genetics/isolation & purification
Remarks:	Separation of amplified V3 region from 16S rDNA by denaturing gradient gel electrophoresis (PCR-DGGE) and 16S-23S rDNA intergenic spacer region polymorphism (ISR-PCR) analyses were tested as tool for differentiation of staphylococcal strains commonly isolated from fermented sausages. Variable V3 regions of 25 staphylococcal reference strains and 96 wild strains of species belonging to the genera Staphylococcus, Micrococcus and Kocuria were analyzed. PCR-DGGE profiles obtained were species-specific for S. sciuri, S. haemolyticus, S. hominis, S. auricularis, S. condimenti, S. kloosi, S. vitulus, S. succinus, S. pasteuri, S. capitis and S. (Macrococcus) caseolyticus. Moreover, 7 groups could be distinguished gathering the remaining species as result of the separation of the V3 rDNA amplicons in DGGE. Furthermore, the combination of the results obtained by PCR-DGGE and ISR-PCR analyses allowed a clear differentiation of all the staphylococcal species analysed, with exception of the pairs S. equorum-S. cohnii and S. carnosus-S. schleiferi. The suitability of both molecular techniques and of the combination their results for the identification of staphylococci was validated analysing partial nucleotide sequence of the 16S rDNA of a representative number of wild strains.
URL:	14529185

Ref #:	81199
Author(s):	Becker,K.;Harmsen,D.;Mellmann,A.;Meier,C.;Schumann,P.;Peters,G.;von Eiff,C.
Journal:	J Clin Microbiol
Title:	Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species
Volume:	42
Page(s):	4988-95
Year:	2004
Keyword(s):	GENBANK/AY688029 GENBANK/AY688030 GENBANK/AY688031 GENBANK/AY688032 GENBANK/AY688033 GENBANK/AY688034 GENBANK/AY688035 GENBANK/AY688036 GENBANK/AY688037 GENBANK/AY688038 GENBANK/AY688039 GENBANK/AY688040 GENBANK/AY688041 GENBANK/AY688042 GENBANK/AY688043 GENBANK/AY688044 GENBANK/AY688045 GENBANK/AY688046 GENBANK/AY688047 GENBANK/AY688048 GENBANK/AY688049 GENBANK/AY688050 GENBANK/AY688051 GENBANK/AY688052 GENBANK/AY688053 GENBANK/AY688054 GENBANK/AY688055 GENBANK/AY688056 GENBANK/AY688057 GENBANK/AY688058 GENBANK/AY688059 GENBANK/AY688060 GENBANK/AY688061 GENBANK/AY688062 GENBANK/AY688063 GENBANK/AY688064 GENBANK/AY688065 GENBANK/AY688066 GENBANK/AY688067 GENBANK/AY688068 GENBANK/AY688069 GENBANK/AY688070 GENBANK/AY688071 GENBANK/AY688072 GENBANK/AY688073 GENBANK/AY688074 GENBANK/AY688075 GENBANK/AY688076 GENBANK/AY688077 GENBANK/AY688078 GENBANK/AY688079 GENBANK/AY688080 GENBANK/AY688081 GENBANK/AY688082 GENBANK/AY688083 GENBANK/AY688084 GENBANK/AY688085 GENBANK/AY688086 GENBANK/AY688087 GENBANK/AY688088 GENBANK/AY688089 GENBANK/AY688090 GENBANK/AY688091 GENBANK/AY688092 GENBANK/AY688093 GENBANK/AY688094 GENBANK/AY688095 GENBANK/AY688096 GENBANK/AY688097 GENBANK/AY688098 GENBANK/AY688099 GENBANK/AY688100 GENBANK/AY688101 GENBANK/AY688102 GENBANK/AY688103 GENBANK/AY688104 GENBANK/AY688105 GENBANK/AY688106 GENBANK/AY688107 GENBANK/AY688108 GENBANK/AY688109 Bacterial Typing Techniques DNA, Bacterial/analysis DNA, Ribosomal/analysis Databases, Nucleic Acid Genes, rRNA Humans Molecular Sequence Data RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Species Specificity Staphylococcal Infections/microbiology Staphylococcus/*classification/genetics/metabolism
Remarks:	To establish an improved ribosomal gene sequence database as part of the Ribosomal Differentiation of Microorganisms (RIDOM) project and to overcome the drawbacks of phenotypic identification systems and publicly accessible sequence databases, both strands of the 5' end of the 16S ribosomal DNA (rDNA) of 81 type and reference strains comprising all validly described staphylococcal (sub)species were sequenced. Assuming a normal distribution for pairwise distances of all unique staphylococcal sequences and choosing a reporting criterion of > or =98.7% similarity for a "distinct species," a statistical error probability of 1.0% was calculated. To evaluate this database, a 16S rDNA fragment (corresponding to Escherichia coli positions 54 to 510) of 55 clinical Staphylococcus isolates (including those of the small-colony variant phenotype) were sequenced and analyzed by the RIDOM approach. Of these isolates, 54 (98.2%) had a similarity score above the proposed threshold using RIDOM; 48 (87.3%) of the sequences gave a perfect match, whereas 83.6% were found by searching National Center for Biotechnology Information (NCBI) database entries. In contrast to RIDOM, which showed four ambiguities at the species level (mainly concerning Staphylococcus intermedius versus Staphylococcus delphini), the NCBI database search yielded 18 taxon-related ambiguities and showed numerous matches exhibiting redundant or unspecified entries. Comparing molecular results with those of biochemical procedures, ID 32 Staph (bioMerieux, Marcy I'Etoile, France) and VITEK 2 (bioMerieux) failed to identify 13 (23.6%) and 19 (34.5%) isolates, respectively, due to incorrect identification and/or categorization below acceptable values. In contrast to phenotypic methods and the NCBI database, the novel high-quality RIDOM sequence database provides excellent identification of staphylococci, including rarely isolated species and phenotypic variants.
URL:	15528685

Data:

(KL 162, DSM 20266) Type strain / CCM, Czechoslovakia in 1976 / Human skin / Schleifer, K. H. & Kloos, W. E. (1975) Int. J. syst. Bact. 25, 50

Accession Date:

01/01/1976

History:

KLOOS W E ,1975 ;CCM ,CZECHOSLOVAKIA ,1976

Authority:

Schleifer and Kloos 1975 (AL)

Depositor:

CCM

Taxonomy:

TaxLink: S2802 (Staphylococcus xylosus Schleifer and Kloos 1975) - Date of change: 5/02/2003

Biosafety Responsibility:

It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

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Product Documents

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Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.