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Bacteria Collection: Sphingomonas paucimobilis

NCTC Number: NCTC 11030
Current Name: Sphingomonas paucimobilis
Original Strain Reference: CL 1/70
Other Collection No: ATCC 29837; DSM 1098; NCPPB 3838
Previous Catalogue Name: Sphingomonas paucimobilis
Type Strain: Yes
Family: Sphingomonadaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, aerobic
Isolated From: inert surfaces;respirator
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS956174
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|U37337|ATCC 29837|Sphingomonas paucimobilis strain ATCC 29837 16S ribosomal RNA (rrn)gene, partial sequence.| tcctggctcagaacg... >gb|U20776|ATCC 29837|Sphingomonas paucimobilis 16S small subunit rRNA gene, partialsequence.| gtattcaccgcggca... >gb|X72722|DSM 1098|S.paucimobilis gene for 16S ribosomal RNA.| tnnnngcatgcctna... >gb|U20776|ATCC 29837|Sphingomonas paucimobilis 16S small subunit ribosomal RNA gene,partial sequence.| acgggtgcgtaacgc... >gb|AM237364|ATCC 29837|OS-64.A|Sphingomonas paucimobilis partial 16S rRNA gene, isolate OS-64.a.| cggctgcctaacaca...
23S rRNA Gene Sequence: >gb|U20776|ATCC 29837|Sphingomonas paucimobilis 16S small subunit rRNA gene, partialsequence.| gtattcaccgcggca... >gb|U20776|ATCC 29837|Sphingomonas paucimobilis 16S small subunit ribosomal RNA gene,partial sequence.| acgggtgcgtaacgc...
Bibliography: HOLMES B ET AL 1977 INT J SYST BACT 27 133
Extended Bibliography: showhide Show bibliography
Ref #: 72460
Author(s): Mueller,J.G.;Devereux,R.;Santavy,D.L.;Lantz,S.E.;Willis,S.G.;Pritchard,P.H.
Journal: Antonie Van Leeuwenhoek
Title: Phylogenetic and physiological comparisons of PAH-degrading bacteria from geographically diverse soils
Volume: 71
Page(s): 329-43
Year: 1997
Keyword(s): GENBANK/U37337 GENBANK/U37348 Bacterial Typing Techniques Biodegradation, Environmental Burkholderia cepacia/metabolism Cell Membrane/chemistry Creosote/metabolism DNA, Bacterial/genetics DNA, Ribosomal/genetics Fluorenes/metabolism Germany Molecular Sequence Data Norway Phenanthrenes/metabolism Phenotype Phylogeny Polycyclic Hydrocarbons, Aromatic/*metabolism Pseudomonadaceae/classification/*metabolism RNA, Ribosomal, 16S/genetics *Soil Microbiology United States
Remarks: The diversity of bacteria isolated from creosote- contaminated soils in the United States, Norway, and Germany was determined by comparing their ability to degrade polycyclic aromatic hydrocarbons (PAHs), their phospholipid ester-linked fatty acid (GC-FAME) profiles, sole carbon source utilization patterns (Biolog assays), and 16S rRNA sequences. Bacteria were initially obtained by enrichment with phenanthrene and fluoranthene. Many were capable of degrading a broad range of the PAHs found in creosote. Phenanthrene- or fluoranthene-degraders were abundant in most of the soils tested. Several of the fluoranthene-degrading isolates clustered with Sphingomonas (formerly Pseudomonas) paucimobilis strain EPA505 in the GC-FAME and Biolog analyses and three of the isolates examined by 16S rRNA sequence comparisons showed a close relationship with Sphingomonas. In addition, the Sphingomonas strains showed the most extensive degradation of 4- & 5-ring PAHs in creosote. Burkholderia cepacia strains isolated on phenanthrene from PAH-contaminated soils had limited ability to attack higher molecular weight PAHs either individually or in creosote. Thus, degradation capabilities appeared to be associated with members of certain taxa, independent of the origin of the soils from which the bacteria were isolated.
URL: 9195008
Ref #: 80226
Author(s): Fredrickson,J.K.;Balkwill,D.L.;Drake,G.R.;Romine,M.F.;Ringelberg,D.B.;White,D.C.
Journal: Appl Environ Microbiol
Title: Aromatic-degrading Sphingomonas isolates from the deep subsurface
Volume: 61
Page(s): 1917-22
Year: 1995
Keyword(s): GENBANK/U20755 GENBANK/U20756 GENBANK/U20772 GENBANK/U20773 GENBANK/U20774 GENBANK/U20775 GENBANK/U20776 Bacteria, Aerobic/classification/*isolation & purification/metabolism Base Sequence Biodegradation, Environmental DNA, Bacterial/genetics DNA, Ribosomal/genetics Fatty Acids/metabolism Hydrocarbons/*metabolism Molecular Sequence Data Phylogeny Polycyclic Compounds/metabolism RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Homology, Nucleic Acid *Soil Microbiology Species Specificity Sphingolipids/metabolism Tennessee
Remarks: An obligately aerobic chemoheterotrophic bacterium (strain F199) previously isolated from Southeast Coastal Plain subsurface sediments and shown to degrade toluene, naphthalene, and other aromatic compounds (J. K. Fredrickson, F. J. Brockman, D. J. Workman, S. W. Li, and T. O. Stevens, Appl. Environ. Microbiol. 57:796-803, 1991) was characterized by analysis of its 16S rRNA nucleotide base sequence and cellular lipid composition. Strain F199 contained 2-OH14:0 and 18:1 omega 7c as the predominant cellular fatty acids and sphingolipids that are characteristic of the genus Sphingomonas. Phylogenetic analysis of its 16S rRNA sequence indicated that F199 was most closely related to Sphingomonas capsulata among the bacteria currently in the Ribosomal Database. Five additional isolates from deep Southeast Coastal Plain sediments were determined by 16S rRNA sequence analysis to be closely related to F199. These strains also contained characteristic sphingolipids. Four of these five strains could also grow on a broad range of aromatic compounds and could mineralize [14C]toluene and [14C]naphthalene. S. capsulata (ATCC 14666), Sphingomonas paucimobilis (ATCC 29837), and one of the subsurface isolates were unable to grow on any of the aromatic compounds or mineralize toluene or naphthalene. These results indicate that bacteria within the genus Sphingomonas are present in Southeast Coastal Plain subsurface sediments and that the capacity for degrading a broad range of substituted aromatic compounds appears to be common among Sphingomonas species from this environment.
URL: 7544095
Ref #: 13724
Author(s): Fredrickson,J.K.;Balkwill,D.L.;Drake,G.R.;Romine,M.F.;Ringelberg,D.B.;White,D.C.
Journal: Appl Environ Microbiol
Title: Aromatic-degrading Sphingomonas isolates from the deep subsurface
Volume: 61
Page(s): 1917-22
Year: 1995
Keyword(s): GENBANK/U20755 GENBANK/U20756 GENBANK/U20772 GENBANK/U20773 GENBANK/U20774 GENBANK/U20775 GENBANK/U20776 Bacteria, Aerobic/classification/*isolation & purification/metabolism Base Sequence Biodegradation Comparative Study DNA, Bacterial/genetics DNA, Ribosomal/genetics Fatty Acids/metabolism Hydrocarbons/*metabolism Molecular Sequence Data Phylogeny Polycyclic Hydrocarbons/metabolism RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Homology, Nucleic Acid *Soil Microbiology Species Specificity Sphingolipids/metabolism Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Tennessee
Remarks: An obligately aerobic chemoheterotrophic bacterium (strain F199) previously isolated from Southeast Coastal Plain subsurface sediments and shown to degrade toluene, naphthalene, and other aromatic compounds (J. K. Fredrickson, F. J. Brockman, D. J. Workman, S. W. Li, and T. O. Stevens, Appl. Environ. Microbiol. 57:796-803, 1991) was characterized by analysis of its 16S rRNA nucleotide base sequence and cellular lipid composition. Strain F199 contained 2-OH14:0 and 18:1 omega 7c as the predominant cellular fatty acids and sphingolipids that are characteristic of the genus Sphingomonas. Phylogenetic analysis of its 16S rRNA sequence indicated that F199 was most closely related to Sphingomonas capsulata among the bacteria currently in the Ribosomal Database. Five additional isolates from deep Southeast Coastal Plain sediments were determined by 16S rRNA sequence analysis to be closely related to F199. These strains also contained characteristic sphingolipids. Four of these five strains could also grow on a broad range of aromatic compounds and could mineralize [14C]toluene and [14C]naphthalene. S. capsulata (ATCC 14666), Sphingomonas paucimobilis (ATCC 29837), and one of the subsurface isolates were unable to grow on any of the aromatic compounds or mineralize toluene or naphthalene. These results indicate that bacteria within the genus Sphingomonas are present in Southeast Coastal Plain subsurface sediments and that the capacity for degrading a broad range of substituted aromatic compounds appears to be common among Sphingomonas species from this environment.
URL: 95374006
Ref #: 13703
Author(s): Mueller,J.G.;Devereux,R.;Santavy,D.L.;Lantz,S.E.;Willis,S.G.;Pritchard,P.H.
Journal: Antonie Van Leeuwenhoek
Title: Phylogenetic and physiological comparisons of PAH-degrading bacteria from geographically diverse soils
Volume: 71
Page(s): 329-43
Year: 1997
Keyword(s): GENBANK/U37337 GENBANK/U37348 Bacterial Typing Techniques Biodegradation Burkholderia cepacia/metabolism Cell Membrane/chemistry Comparative Study Creosote/metabolism DNA, Bacterial/genetics DNA, Ribosomal/genetics Fluorenes/metabolism Germany Molecular Sequence Data Norway Phenanthrenes/metabolism Phenotype Phylogeny Polycyclic Hydrocarbons, Aromatic/*metabolism Pseudomonadaceae/classification/*metabolism RNA, Ribosomal, 16S/genetics *Soil Microbiology Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. United States
Remarks: The diversity of bacteria isolated from creosote- contaminated soils in the United States, Norway, and Germany was determined by comparing their ability to degrade polycyclic aromatic hydrocarbons (PAHs), their phospholipid ester-linked fatty acid (GC-FAME) profiles, sole carbon source utilization patterns (Biolog assays), and 16S rRNA sequences. Bacteria were initially obtained by enrichment with phenanthrene and fluoranthene. Many were capable of degrading a broad range of the PAHs found in creosote. Phenanthrene- or fluoranthene-degraders were abundant in most of the soils tested. Several of the fluoranthene-degrading isolates clustered with Sphingomonas (formerly Pseudomonas) paucimobilis strain EPA505 in the GC-FAME and Biolog analyses and three of the isolates examined by 16S rRNA sequence comparisons showed a close relationship with Sphingomonas. In addition, the Sphingomonas strains showed the most extensive degradation of 4- & 5-ring PAHs in creosote. Burkholderia cepacia strains isolated on phenanthrene from PAH-contaminated soils had limited ability to attack higher molecular weight PAHs either individually or in creosote. Thus, degradation capabilities appeared to be associated with members of certain taxa, independent of the origin of the soils from which the bacteria were isolated.
URL: 97338448
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 1775
Author(s): Yabuuchi,E.etal.
Journal: J. Gen. Appl. Microbiol.
Title: Flavobacterium devorans ATCC 10829: a strain of Pseudomonas paucimobilis.
Volume: 25
Page(s): 95-107
Year: 1979
Ref #: 1779
Author(s): Oyaizu,H.;Komagata,K.
Journal: J. Gen. Appl. Microbiol.
Title: Chemotaxonomic and phenotypic characterization of the strains of species in the Flavobacterium-Cytophaga complex.
Volume: 27
Page(s): 57-107
Year: 1981
Ref #: 1795
Author(s): Jenkins,C.L.etal.
Journal: Curr. Microbiol.
Title: The pigment of Pseudomonas paucimobilis is a carotenoid (Nostoxanthin), rather than a brominated acryl-polyene (Xanthomonadin).
Volume: 3
Page(s): 1-4
Year: 1979
Ref #: 2058
Author(s): Holmes,B.;Owen,R.J.;Evans,A.;Malnick,H.;Willcox,W.R.
Journal: Int. J. Syst. Bacteriol.
Title: Pseudomonas paucimobilis, a new species isolated from human clinical specimens, the hospital environment, and other sources.
Volume: 27
Page(s): 133-146
Year: 1977
Ref #: 4029
Author(s): Owen,R.J.;Jackman,P.J.H.
Journal: J. Gen. Microbiol.
Title: The similarities between Pseudomonas paucimobilis and allied bacteria derived from analysis of DNA and electrophoretic protein patterns.
Volume: 128
Page(s): 2945-2954
Year: 1982
Data: (120039, Allen) Type strain / I. Phillips, St. Thomas Hospital, London in 1969 / Respirator / Holmes, B. et al. (1977) Int. J. syst. Bact. 27, 133
Accession Date: 01/01/1969
History: ISOLATED BY PHILLIPS I ST THOMAS HOSPITAL LONDON
Authority: (Holmes et al. 1977) Yabuuchi et al. 1990
Depositor: PHILLIPS I
Taxonomy: TaxLink: S3893 (Pseudomonas paucimobilis) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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