Extended Bibliography: |
Show bibliography
Ref #: |
95542 |
Author(s): |
Rossau,R.;Duhamel,M.;Jannes,G.;Decourt,J.L.;Van Heuverswyn,H. |
Journal: |
J Gen Microbiol |
Title: |
The development of specific rRNA-derived oligonucleotide probes for Haemophilus ducreyi, the causative agent of chancroid |
Volume: |
137 |
Page(s): |
277-85 |
Year: |
1991 |
Keyword(s): |
GENBANK/M38751
GENBANK/M55252
GENBANK/M59337
GENBANK/M59338
GENBANK/M59339
GENBANK/M59340
GENBANK/M59341
GENBANK/M59342
GENBANK/M59343
GENBANK/M59344
GENBANK/M63900
Base Sequence
Chancroid/microbiology
Cloning, Molecular
Haemophilus ducreyi/*classification/genetics
Humans
Molecular Sequence Data
Nucleic Acid Conformation
Nucleic Acid Hybridization
*Oligonucleotide Probes
Polymerase Chain Reaction
RNA, Bacterial/genetics
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/*genetics
Sequence Alignment
|
Remarks: |
Part of a ribosomal ribonucleic acid (rRNA) cistron of Haemophilus ducreyi was enzymically amplified using conserved primers within the rRNA molecules, cloned in a plasmid vector, and sequenced. From the nucleotide sequence, eight oligonucleotides complementary to different regions in the 16S and 23S rRNA molecules were selected, chemically synthesized, and used as hybridization probes. Hybridization experiments with at least 41 H. ducreyi strains and 13 or 14 non-H. ducreyi strains revealed that all eight oligonucleotide probes were highly reliable and completely specific for H. ducreyi strains. Comparisons of 16S rRNA sequences confirm that H. ducreyi is a member of the Pasteurellaceae though not closely related to other species in this family. |
URL: |
1707945 |
|
Ref #: |
95523 |
Author(s): |
Gu,X.X.;Rossau,R.;Jannes,G.;Ballard,R.;Laga,M.;Van Dyck,E. |
Journal: |
Microbiology |
Title: |
The rrs (16S)-rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay |
Volume: |
144 ( Pt 4) |
Page(s): |
1013-9 |
Year: |
1998 |
Keyword(s): |
GENBANK/Y11717
GENBANK/Y11738
Base Sequence
Chancroid/diagnosis/microbiology
DNA, Ribosomal/*isolation & purification
Genes, Bacterial/*genetics
Haemophilus ducreyi/genetics/*isolation & purification
Humans
Molecular Sequence Data
Polymerase Chain Reaction/*methods
Sequence Alignment
Sequence Analysis, DNA
|
Remarks: |
The intergenic spacer region between the rrs and rrl ribosomal RNA genes of Haemophilus ducreyi was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of H. ducreyi was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods. These results indicate that this PCR assay has the potential to become an accurate and easy reference method for the detection of H. ducreyi. |
URL: |
9579075 |
|
Ref #: |
12800 |
Author(s): |
Rossau,R.;Duhamel,M.;Jannes,G.;Decourt,J.L.;Van Heuverswyn,H. |
Journal: |
J Gen Microbiol |
Title: |
The development of specific rRNA-derived oligonucleotide probes for Haemophilus ducreyi, the causative agent of chancroid |
Volume: |
137 ( Pt 2) |
Page(s): |
277-85 |
Year: |
1991 |
Keyword(s): |
GENBANK/M38751
GENBANK/M55252
GENBANK/M59337
GENBANK/M59338
GENBANK/M59339
GENBANK/M59340
GENBANK/M59341
GENBANK/M59342
GENBANK/M59343
GENBANK/M59344
GENBANK/M63900
Base Sequence
Chancroid/microbiology
Cloning, Molecular
Haemophilus ducreyi/*classification/genetics
Human
Molecular Sequence Data
Nucleic Acid Conformation
Nucleic Acid Hybridization
*Oligonucleotide Probes
Polymerase Chain Reaction
RNA, Bacterial/genetics
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/*genetics
Sequence Alignment
|
Remarks: |
Part of a ribosomal ribonucleic acid (rRNA) cistron of Haemophilus ducreyi was enzymically amplified using conserved primers within the rRNA molecules, cloned in a plasmid vector, and sequenced. From the nucleotide sequence, eight oligonucleotides complementary to different regions in the 16S and 23S rRNA molecules were selected, chemically synthesized, and used as hybridization probes. Hybridization experiments with at least 41 H. ducreyi strains and 13 or 14 non-H. ducreyi strains revealed that all eight oligonucleotide probes were highly reliable and completely specific for H. ducreyi strains. Comparisons of 16S rRNA sequences confirm that H. ducreyi is a member of the Pasteurellaceae though not closely related to other species in this family. |
URL: |
91202097 |
|
Ref #: |
13718 |
Author(s): |
Gu,X.X.;Rossau,R.;Jannes,G.;Ballard,R.;Laga,M.;Van Dyck,E. |
Journal: |
Microbiology |
Title: |
The rrs (16S)-rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay |
Volume: |
144 ( Pt 4) |
Page(s): |
1013-9 |
Year: |
1998 |
Keyword(s): |
GENBANK/Y11717
GENBANK/Y11738
Base Sequence
Chancroid/diagnosis/microbiology
DNA, Ribosomal/*isolation & purification
Genes, Bacterial/*genetics
Haemophilus ducreyi/genetics/*isolation & purification
Human
Molecular Sequence Data
Polymerase Chain Reaction/*methods
Sequence Alignment
Sequence Analysis, DNA
Support, Non-U.S. Gov't
|
Remarks: |
The intergenic spacer region between the rrs and rrl ribosomal RNA genes of Haemophilus ducreyi was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of H. ducreyi was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods. These results indicate that this PCR assay has the potential to become an accurate and easy reference method for the detection of H. ducreyi. |
URL: |
98240238 |
|
Ref #: |
1300 |
Author(s): |
Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed) |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Approved Lists of Bacterial Names. |
Volume: |
30 |
Page(s): |
225-420 |
Year: |
1980 |
|
Ref #: |
5066 |
Author(s): |
Carlone,G.M.;Schalla,W.O.;Moss,C.W.;Ashley,D.L.;Fast,D.M.;Holler,J.S.;Pjikaytis,B.D. |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Haemophilus ducreyi isoprenoid quinone content and structure determination. |
Volume: |
38 |
Page(s): |
249-253 |
Year: |
1988 |
|
Ref #: |
5067 |
Author(s): |
Casin,I.;Grimont,F.;Grimont,P.A.D.;Sanson-LePors,M.-J. |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Lack of deoxyribonucleic acid relatedness between Haemophilus ducreyi and other Haemophilus species. |
Volume: |
35 |
Page(s): |
23-25 |
Year: |
1985 |
|
Ref #: |
7016 |
Author(s): |
Busse,H.-J.;Bunka,S.;Hensel,A.;Lubitz,W. |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Discrimination of members of the family Pasteurellaceae based on polyamine patterns. |
Volume: |
47 |
Page(s): |
698-708 |
Year: |
1997 |
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