Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Pseudomonas mendocina

NCTC Number: NCTC 10899
Current Name: Pseudomonas mendocina
Original Strain Reference: CH 120
Other Collection No: ATCC 25413; CH 120
Previous Catalogue Name: Pseudomonas mendocina
Type Strain: No
Family: Pseudomonadaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37,, aerobic
Isolated From: freshwater
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1043808
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AJ006109|ATCC 25413|Pseudomonas mendocina 16S rRNA gene, strain ATCC 25413, partial.| tttgatcctggctca...
Extended Bibliography: showhide Show bibliography
Ref #: 95512
Author(s): Sikorski,J.;Rossello-Mora,R.;Lorenz,M.G.
Journal: Syst Appl Microbiol
Title: Analysis of genotypic diversity and relationships among Pseudomonas stutzeri strains by PCR-based genomic fingerprinting and multilocus enzyme electrophoresis
Volume: 22
Page(s): 393-402
Year: 1999
Keyword(s): GENBANK/AJ005167 GENBANK/AJ006103 GENBANK/AJ006104 GENBANK/AJ006105 GENBANK/AJ006106 GENBANK/AJ006107 GENBANK/AJ006108 GENBANK/AJ006109 GENBANK/AJ006110 Base Sequence Electrophoresis/*methods Genotype Humans Molecular Sequence Data Phylogeny Polymorphism, Genetic/genetics Pseudomonas/classification/enzymology/*genetics RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics *Random Amplified Polymorphic DNA Technique Seawater/microbiology *Soil Microbiology Species Specificity *Variation (Genetics) Waste Disposal, Fluid *Water Microbiology
Remarks: Molecular fingerprinting procedures including random amplified polymorphic DNA-PCR (RAPD), repetitive extragenic palindromic PCR (rep-PCR) with REP, ERIC, and BOX primers and multilocus enzyme electrophoresis (MLEE) were used for genotypic characterization of 16 P. stutzeri strains originally isolated from marine, waste water, clinical and soil samples. A distinct genotype of each strain and overall great genotypic diversity were found within P. stutzeri. Cluster analysis (UPGMA) of the electrophoretic patterns of all PCR-based methods used resulted in concordant grouping of 8 strains. With the other strains conflicting clustering was noticed. The variability of clustering in PCR-based analyses suggested the occurrence of chromosomal rearrangements. When RAPD-, rep-PCR and MLEE fingerprints were used in a cluster analysis of combined electrophoretic patterns, the P. stutzeri strains could be differentiated into seven distinct genotypic groups. These results supported the subdivision of the species in several genomovars and reproduced, with higher resolution, the strain grouping after 16S rRNA phylogenetic reconstruction. The combined use of several fingerprint-based genotypic analyses results in higher resolutive strain clustering by UPGMA than each of the single ones analyzed separately. Additionally, this combination of individual typings proved to be reliable of the determination of the great genotypic diversity and relationships among the P. stutzeri strains.
URL: 10553292
Ref #: 13709
Author(s): Sikorski,J.;Rossello-Mora,R.;Lorenz,M.G.
Journal: Syst Appl Microbiol
Title: Analysis of genotypic diversity and relationships among Pseudomonas stutzeri strains by PCR-based genomic fingerprinting and multilocus enzyme electrophoresis
Volume: 22
Page(s): 393-402
Year: 1999
Keyword(s): GENBANK/AJ005167 GENBANK/AJ006103 GENBANK/AJ006104 GENBANK/AJ006105 GENBANK/AJ006106 GENBANK/AJ006107 GENBANK/AJ006108 GENBANK/AJ006109 GENBANK/AJ006110 Base Sequence Comparative Study Electrophoresis/*methods Genotype Human Molecular Sequence Data Phylogeny Polymorphism (Genetics)/genetics Pseudomonas/classification/enzymology/*genetics RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics *Random Amplified Polymorphic DNA Technique Seawater/microbiology *Soil Microbiology Species Specificity Support, Non-U.S. Gov't *Variation (Genetics) Waste Disposal, Fluid *Water Microbiology
Remarks: Molecular fingerprinting procedures including random amplified polymorphic DNA-PCR (RAPD), repetitive extragenic palindromic PCR (rep-PCR) with REP, ERIC, and BOX primers and multilocus enzyme electrophoresis (MLEE) were used for genotypic characterization of 16 P. stutzeri strains originally isolated from marine, waste water, clinical and soil samples. A distinct genotype of each strain and overall great genotypic diversity were found within P. stutzeri. Cluster analysis (UPGMA) of the electrophoretic patterns of all PCR-based methods used resulted in concordant grouping of 8 strains. With the other strains conflicting clustering was noticed. The variability of clustering in PCR-based analyses suggested the occurrence of chromosomal rearrangements. When RAPD-, rep-PCR and MLEE fingerprints were used in a cluster analysis of combined electrophoretic patterns, the P. stutzeri strains could be differentiated into seven distinct genotypic groups. These results supported the subdivision of the species in several genomovars and reproduced, with higher resolution, the strain grouping after 16S rRNA phylogenetic reconstruction. The combined use of several fingerprint-based genotypic analyses results in higher resolutive strain clustering by UPGMA than each of the single ones analyzed separately. Additionally, this combination of individual typings proved to be reliable of the determination of the great genotypic diversity and relationships among the P. stutzeri strains.
URL: 20020862
Data: (ATCC 25413) ATCC in 1972 / N. J. Palleroni / Water/ Palleroni, N. J. et al. (1970) J. gen. Microbiol. 60, 215
Accession Date: 01/01/1972
History: ISOLATED BY PALLERONI N J
Authority: Palleroni 1970 (AL)
Depositor: ATCC
Taxonomy: TaxLink: S2461 (Pseudomonas mendocina Palleroni 1970) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

Note: Links open in a new window

Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

Contact us if you want to discuss commercial use of the cultures.

Available Formats

Ampoule (Bacteria)

Back to top
Copyright © Public Health England.

Please confirm your country of origin from the list below.