Bacteria Collection: Pseudomonas pseudoalcaligenes subsp. pseudoalcaligenes

NCTC Number:	NCTC 10860
Current Name:	Pseudomonas pseudoalcaligenes subsp. pseudoalcaligenes
Original Strain Reference:	Stanier 63
Other Collection No:	ATCC 17440; DSM 50188; ICPB 2750-63; IMET 11177; JCM 5968; NCIB 9946; NCPPB 1971; STANIER 63; THIBAULT X-59
Previous Catalogue Name:	Pseudomonas pseudoalcaligenes subsp. pseudoalcaligenes
Type Strain:	Yes
Family:	Pseudomonadaceae
Hazard Group (ACDP):	2
Release Restrictions:	Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media:	Nutrient agar, 48 hours, 30°C, aerobic
Conditions for growth on liquid media:	nutrient broth,30, aerobic
Isolated From:	human, sinus drainage
Whole Genome Sequence:	http://www.ebi.ac.uk/ena/data/view/ERS1018580
Annotated Genome:	ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
Bibliography:	STANIER R Y ET AL 1966 J GEN MICROBIOL 43 159
Extended Bibliography:	Show bibliography Ref #: 95440 Author(s): Guasp,C.;Moore,E.R.;Lalucat,J.;Bennasar,A. Journal: Int J Syst Evol Microbiol Title: Utility of internally transcribed 16S-23S rDNA spacer regions for the definition of Pseudomonas stutzeri genomovars and other Pseudomonas species Volume: 50 Pt 4 Page(s): 1629-39 Year: 2000 Keyword(s): GENBANK/AJ251900 GENBANK/AJ251901 GENBANK/AJ251902 GENBANK/AJ251903 GENBANK/AJ251904 GENBANK/AJ251905 GENBANK/AJ251906 GENBANK/AJ251907 GENBANK/AJ251908 GENBANK/AJ251909 GENBANK/AJ251910 GENBANK/AJ279238 GENBANK/AJ279239 GENBANK/AJ279240 GENBANK/AJ279241 GENBANK/AJ279242 GENBANK/AJ279243 GENBANK/AJ279244 GENBANK/AJ279245 GENBANK/AJ390581 GENBANK/AJ390582 GENBANK/AJ390583 GENBANK/AJ390584 GENBANK/AJ390585 GENBANK/AJ390586 GENBANK/AJ390587 GENBANK/AJ390588 GENBANK/D86356 GENBANK/L28148 GENBANK/L28159 etc. DNA, Bacterial/analysis DNA, Ribosomal Spacer/*analysis Environmental Microbiology Genotype Humans Nucleic Acid Hybridization Phylogeny Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Pseudomonas/*classification/genetics RNA, Ribosomal, 16S/analysis RNA, Ribosomal, 23S/analysis Sequence Homology, Nucleic Acid Remarks: Bacteria identified and classified as Pseudomonas stutzeri, on the basis of traditional criteria, are recognized to be markedly heterogeneous, such that a systematic phenotypic characterization has not been correlated with genotypic groupings (i.e. genomovars) based upon DNA-DNA similarities. The internally transcribed 16S-23S rDNA spacer (ITS1) regions of P. stutzeri were analysed with respect to the ability of these nucleic acid regions to differentiate and identify the genomic groups (i.e. genomovars) of P. stutzeri. The ITS1s of 34 strains of P. stutzeri were amplified by PCR and the PCR product was subjected to RFLP analysis, which allowed the differentiation and identification of the strains to their respective genomovars. Sequence determination and analysis of ITS1s supported further the results obtained by RFLP, i.e. nucleotide signatures were identified in strains belonging to different genomovars. The ITS1s of all strains of P. stutzeri contained the tandem tRNA(Ile)/tRNA(Ala) genes and did not exhibit distinct sequence heterogeneity between different operons of a strain. Phylogenetically informative variable sites were located, exclusively, in non-coding regions. The results of the RFLP and sequence analysis of ITS1s supported and correlated with the phylogenetic relationships estimated from 16S rRNA gene sequence comparisons and DNA-DNA hybridizations, offering an alternative tool for genomovar and species differentiation. URL: 10939670 Ref #: 13706 Author(s): Guasp,C.;Moore,E.R.;Lalucat,J.;Bennasar,A. Journal: Int J Syst Evol Microbiol Title: Utility of internally transcribed 16S-23S rDNA spacer regions for the definition of Pseudomonas stutzeri genomovars and other Pseudomonas species Volume: 50 Pt 4 Page(s): 1629-39 Year: 2000 Keyword(s): GENBANK/AJ251900 GENBANK/AJ251901 GENBANK/AJ251902 GENBANK/AJ251903 GENBANK/AJ251904 GENBANK/AJ251905 GENBANK/AJ251906 GENBANK/AJ251907 GENBANK/AJ251908 GENBANK/AJ251909 GENBANK/AJ251910 GENBANK/AJ279238 GENBANK/AJ279239 GENBANK/AJ279240 GENBANK/AJ279241 GENBANK/AJ279242 GENBANK/AJ279243 GENBANK/AJ279244 GENBANK/AJ279245 GENBANK/AJ390581 GENBANK/AJ390582 GENBANK/AJ390583 GENBANK/AJ390584 GENBANK/AJ390585 GENBANK/AJ390586 GENBANK/AJ390587 GENBANK/AJ390588 GENBANK/D86356 GENBANK/L28148 GENBANK/L28159 etc. DNA, Bacterial/analysis DNA, Ribosomal Spacer/*analysis Environmental Microbiology Genotype Human Nucleic Acid Hybridization Phylogeny Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Pseudomonas/*classification/genetics RNA, Ribosomal, 16S/analysis RNA, Ribosomal, 23S/analysis Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't Remarks: Bacteria identified and classified as Pseudomonas stutzeri, on the basis of traditional criteria, are recognized to be markedly heterogeneous, such that a systematic phenotypic characterization has not been correlated with genotypic groupings (i.e. genomovars) based upon DNA-DNA similarities. The internally transcribed 16S-23S rDNA spacer (ITS1) regions of P. stutzeri were analysed with respect to the ability of these nucleic acid regions to differentiate and identify the genomic groups (i.e. genomovars) of P. stutzeri. The ITS1s of 34 strains of P. stutzeri were amplified by PCR and the PCR product was subjected to RFLP analysis, which allowed the differentiation and identification of the strains to their respective genomovars. Sequence determination and analysis of ITS1s supported further the results obtained by RFLP, i.e. nucleotide signatures were identified in strains belonging to different genomovars. The ITS1s of all strains of P. stutzeri contained the tandem tRNA(Ile)/tRNA(Ala) genes and did not exhibit distinct sequence heterogeneity between different operons of a strain. Phylogenetically informative variable sites were located, exclusively, in non-coding regions. The results of the RFLP and sequence analysis of ITS1s supported and correlated with the phylogenetic relationships estimated from 16S rRNA gene sequence comparisons and DNA-DNA hybridizations, offering an alternative tool for genomovar and species differentiation. URL: 20393664 Ref #: 1300 Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed) Journal: Int. J. Syst. Bacteriol. Title: Approved Lists of Bacterial Names. Volume: 30 Page(s): 225-420 Year: 1980 Ref #: 171 Author(s): Stanier,R.Y.;Palleroni,N.J.;DoudoroffM. Journal: J. Gen. Microbiol. Title: The aerobic pseudomonads: a taxonomic study. Volume: 43 Page(s): 159-275 Year: 1966 Ref #: 4603 Author(s): Gavini,F.;Holmes,G.;Izard,D.;Beji,A.;Bernigaud,A.;Jakubczak,E. Journal: Int. J. Syst. Bacteriol. Title: Numerical taxonomy of Pseudomonas alcaligenes, P. pseudoalcaligenes, P. mendocina, P. stutzeri, and related bacteria. Volume: 39 Page(s): 135-144 Year: 1989
Data:	(Thibault X-59, NCPPB 1971, ATCC 17440) Type strain / ATCC in 1972 / R. Y. Stanier / P. Thibault / Sinus drainage / Stanier, R. Y. et al. (1966) J. gen. Microbiol. 43, 159
Accession Date:	01/01/1972
History:	THIBAULT P - STANIER R Y
Authority:	Stanier 1966 (AL)
Depositor:	ATCC
Taxonomy:	TaxLink: S2480 (Pseudomonas pseudoalcaligenes subsp. pseudoalcaligenes Stanier 1966) - Date of change: 5/02/2003
Biosafety Responsibility:	It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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