Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Staphylococcus aureus

NCTC Number: NCTC 10833
Current Name: Staphylococcus aureus
Original Strain Reference: Newman D2C
Other Collection No: ATCC 25904; NEWMAN D2C
Previous Catalogue Name: Staphylococcus aureus
Type Strain: No
Family: Staphylococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: not recorded
Whole Genome Sequence:
Annotated Genome:
16S rRNA Gene Sequence: >gb|AY688032|ATCC 25904|Staphylococcus aureus subsp. aureus strain ATCC 25904 16S ribosomalRNA gene, partial sequence.| catgcaagtcgagcg...
Bibliography: HAWIGER J ET.AL. 1970 J.LAB.CLIN.MED. 75 93
Extended Bibliography: showhide Show bibliography
Ref #: 81199
Author(s): Becker,K.;Harmsen,D.;Mellmann,A.;Meier,C.;Schumann,P.;Peters,G.;von Eiff,C.
Journal: J Clin Microbiol
Title: Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species
Volume: 42
Page(s): 4988-95
Year: 2004
Keyword(s): GENBANK/AY688029 GENBANK/AY688030 GENBANK/AY688031 GENBANK/AY688032 GENBANK/AY688033 GENBANK/AY688034 GENBANK/AY688035 GENBANK/AY688036 GENBANK/AY688037 GENBANK/AY688038 GENBANK/AY688039 GENBANK/AY688040 GENBANK/AY688041 GENBANK/AY688042 GENBANK/AY688043 GENBANK/AY688044 GENBANK/AY688045 GENBANK/AY688046 GENBANK/AY688047 GENBANK/AY688048 GENBANK/AY688049 GENBANK/AY688050 GENBANK/AY688051 GENBANK/AY688052 GENBANK/AY688053 GENBANK/AY688054 GENBANK/AY688055 GENBANK/AY688056 GENBANK/AY688057 GENBANK/AY688058 GENBANK/AY688059 GENBANK/AY688060 GENBANK/AY688061 GENBANK/AY688062 GENBANK/AY688063 GENBANK/AY688064 GENBANK/AY688065 GENBANK/AY688066 GENBANK/AY688067 GENBANK/AY688068 GENBANK/AY688069 GENBANK/AY688070 GENBANK/AY688071 GENBANK/AY688072 GENBANK/AY688073 GENBANK/AY688074 GENBANK/AY688075 GENBANK/AY688076 GENBANK/AY688077 GENBANK/AY688078 GENBANK/AY688079 GENBANK/AY688080 GENBANK/AY688081 GENBANK/AY688082 GENBANK/AY688083 GENBANK/AY688084 GENBANK/AY688085 GENBANK/AY688086 GENBANK/AY688087 GENBANK/AY688088 GENBANK/AY688089 GENBANK/AY688090 GENBANK/AY688091 GENBANK/AY688092 GENBANK/AY688093 GENBANK/AY688094 GENBANK/AY688095 GENBANK/AY688096 GENBANK/AY688097 GENBANK/AY688098 GENBANK/AY688099 GENBANK/AY688100 GENBANK/AY688101 GENBANK/AY688102 GENBANK/AY688103 GENBANK/AY688104 GENBANK/AY688105 GENBANK/AY688106 GENBANK/AY688107 GENBANK/AY688108 GENBANK/AY688109 *Bacterial Typing Techniques DNA, Bacterial/analysis DNA, Ribosomal/analysis *Databases, Nucleic Acid Genes, rRNA Humans Molecular Sequence Data RNA, Ribosomal, 16S/*genetics Sequence Analysis, DNA Species Specificity Staphylococcal Infections/*microbiology Staphylococcus/*classification/genetics/metabolism
Remarks: To establish an improved ribosomal gene sequence database as part of the Ribosomal Differentiation of Microorganisms (RIDOM) project and to overcome the drawbacks of phenotypic identification systems and publicly accessible sequence databases, both strands of the 5' end of the 16S ribosomal DNA (rDNA) of 81 type and reference strains comprising all validly described staphylococcal (sub)species were sequenced. Assuming a normal distribution for pairwise distances of all unique staphylococcal sequences and choosing a reporting criterion of > or =98.7% similarity for a "distinct species," a statistical error probability of 1.0% was calculated. To evaluate this database, a 16S rDNA fragment (corresponding to Escherichia coli positions 54 to 510) of 55 clinical Staphylococcus isolates (including those of the small-colony variant phenotype) were sequenced and analyzed by the RIDOM approach. Of these isolates, 54 (98.2%) had a similarity score above the proposed threshold using RIDOM; 48 (87.3%) of the sequences gave a perfect match, whereas 83.6% were found by searching National Center for Biotechnology Information (NCBI) database entries. In contrast to RIDOM, which showed four ambiguities at the species level (mainly concerning Staphylococcus intermedius versus Staphylococcus delphini), the NCBI database search yielded 18 taxon-related ambiguities and showed numerous matches exhibiting redundant or unspecified entries. Comparing molecular results with those of biochemical procedures, ID 32 Staph (bioMerieux, Marcy I'Etoile, France) and VITEK 2 (bioMerieux) failed to identify 13 (23.6%) and 19 (34.5%) isolates, respectively, due to incorrect identification and/or categorization below acceptable values. In contrast to phenotypic methods and the NCBI database, the novel high-quality RIDOM sequence database provides excellent identification of staphylococci, including rarely isolated species and phenotypic variants.
URL: 15528685
Data: (ATCC 25904) ATCC in 1972 / J. Hawiger / E. S. Duthie / Measurement of fibrinogen in serum / Hawiger, J. et al. (1970) J. lab. clin. Med. 75, 93
Accession Date: 01/01/1972
Authority: Rosenbach 1884 (AL)
Depositor: ATCC
Taxonomy: TaxLink: S2775 (Staphylococcus aureus subsp. aureus Rosenbach 1884) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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