Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Actinobacillus rossii

NCTC Number: NCTC 10801
Current Name: Actinobacillus rossii
Original Strain Reference: 192
Other Collection No: ATCC 27072; 192
Previous Catalogue Name: Actinobacillus rossii
Type Strain: Yes
Family: Pasteurellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient agar,37, facultative anaerobe
Isolated From: mammal, vaginal secretions,sow
Whole Genome Sequence:
16S rRNA Gene Sequence: >gb|AY362895|ATCC 27072|Actinobacillus rossii strain ATCC 27072 16S ribosomal RNA gene,partial sequence.| attgaacgctggcgg...
Bibliography: ROSS R F ET AL 1972 INT J SYST BACT 22 39
Extended Bibliography: showhide Show bibliography
Ref #: 95477
Author(s): Korczak,B.;Christensen,H.;Emler,S.;Frey,J.;Kuhnert,P.
Journal: Int J Syst Evol Microbiol
Title: Phylogeny of the family Pasteurellaceae based on rpoB sequences
Volume: 54
Page(s): 1393-9
Year: 2004
Keyword(s): Bacterial Proteins/genetics DNA, Bacterial/chemistry/isolation & purification DNA, Ribosomal/chemistry DNA-Directed RNA Polymerases/*genetics Genes, rRNA Molecular Sequence Data Pasteurellaceae/*classification/*genetics Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Sequence Homology
Remarks: Sequences of the gene encoding the beta-subunit of the RNA polymerase (rpoB) were used to delineate the phylogeny of the family Pasteurellaceae. A total of 72 strains, including the type strains of the major described species as well as selected field isolates, were included in the study. Selection of universal rpoB-derived primers for the family allowed straightforward amplification and sequencing of a 560 bp fragment of the rpoB gene. In parallel, 16S rDNA was sequenced from all strains. The phylogenetic tree obtained with the rpoB sequences reflected the major branches of the tree obtained with the 16S rDNA, especially at the genus level. Only a few discrepancies between the trees were observed. In certain cases the rpoB phylogeny was in better agreement with DNA-DNA hybridization studies than the phylogeny derived from 16S rDNA. The rpoB gene is strongly conserved within the various species of the family of Pasteurellaceae. Hence, rpoB gene sequence analysis in conjunction with 16S rDNA sequencing is a valuable tool for phylogenetic studies of the Pasteurellaceae and may also prove useful for reorganizing the current taxonomy of this bacterial family.
URL: 15280320
Data: (ATCC 27072) R. F. Ross, Iowa in 1971 / Vagina, sow / Ross, R. F. et al. (1972) Int. J. syst. Bact. 22, 39
Accession Date: 01/01/1971
Authority: SNEATH and STEVENS 1990
Depositor: ROSS R F
Taxonomy: TaxLink: S3826 (Actinobacillus rossii sneath and stevens 1990 emend. christensen et al. 2005) - Date of change: 16/06/2007 by NCTCUp to 16/06/2007: G77 (Actinobacillus Brumpt 1910) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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