Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Mycobacterium rhodesiae

NCTC Number: NCTC 10779
Current Name: Mycobacterium rhodesiae
Original Strain Reference: 5295
Other Collection No: ATCC 27024; DSM 44223; JCM 6363; 5295
Previous Catalogue Name: Mycobacterium rhodesiae
Type Strain: Yes
Family: Mycobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Lowenstein-Jensen slopes, 2 - 7 days, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, aerobic
Isolated From: human, sputum
Whole Genome Sequence:
16S rRNA Gene Sequence: >gb|AJ429047|DSM 44223T|Mycobacterium rhodesiae 16S rRNA gene, strain DSM 44223T.| tggctcaggacgaac...
23S rRNA Gene Sequence: >gb|U24544|ATCC 27024|Mycobacterium rhodesiae 23S rRNA, partial sequence.| tgcgcttacaatccg...
Bibliography: TSUKAMURA M ET AL 1971 JAP J MICROBIOL 15 407
Extended Bibliography: showhide Show bibliography
Ref #: 75210
Author(s): Stone,B.B.;Nietupski,R.M.;Breton,G.L.;Weisburg,W.G.
Journal: Int J Syst Bacteriol
Title: Comparison of Mycobacterium 23S rRNA sequences by high-temperature reverse transcription and PCR
Volume: 45
Page(s): 811-9
Year: 1995
Keyword(s): GENBANK/U24502 GENBANK/U24503 GENBANK/U24504 GENBANK/U24505 GENBANK/U24506 GENBANK/U24507 GENBANK/U24508 GENBANK/U24509 GENBANK/U24510 GENBANK/U24511 GENBANK/U24512 GENBANK/U24513 GENBANK/U24514 GENBANK/U24515 GENBANK/U24516 GENBANK/U24517 GENBANK/U24518 GENBANK/U24519 GENBANK/U24520 GENBANK/U24521 GENBANK/U24522 GENBANK/U24523 GENBANK/U24524 GENBANK/U24525 GENBANK/U24526 GENBANK/U24527 GENBANK/U24528 GENBANK/U24529 GENBANK/U24530 GENBANK/U24531 Base Sequence Molecular Sequence Data Mycobacterium/*genetics Phylogeny *Polymerase Chain Reaction RNA, Bacterial/*chemistry RNA, Ribosomal, 16S/chemistry RNA, Ribosomal, 23S/*chemistry Temperature Transcription, Genetic
Remarks: We describe a modified rRNA sequence analysis method which we used to determine the phylogenetic relationships among 58 species belonging to the genus Mycobacterium. We combined the sensitivity of the reverse transcriptase PCR for amplifying nanogram amounts of template rRNA material with the elevated extension temperatures used for the thermostable DNA polymerase from Thermus thermophilus. A 70 degrees C reverse transcription extension step permitted improved read-through of highly structured rRNA templates from members of the genus Mycobacterium, which have G+C contents of 66 to 71 mol%. The nucleic acid sequences of the amplified material were then determined by performing thermal cycle sequencing with alpha-33P-labeled primers, again with extension at 70 degrees C. Nonspecifically terminated bands were chased by using terminal deoxynucleotidyl transferase. Our method had a template requirement of nanogram amounts or less of purified RNA or 2,000 CFU of intact cells and had sufficient sensitivity so that lyophils obtained from the American Type Culture Collection could be used as source material. Sequences from a 250-nucleotide stretch of the 23S rRNA were aligned, and phylogenetic trees were evaluated by using the De Soete distance treeing algorithm and Rhodococcus bronchialis as the outgroup. Our 23S rRNA trees were compared with previously published 16S rRNA trees, including the comprehensive trees developed by the University of Illinois Ribosomal Database Project, and included 15 species not evaluated previously. Most of the groups were in general agreement and were consistent with relationships determined on the basis of biochemical characteristics, but some new relationships were also observed.
URL: 7547304
Ref #: 5147
Author(s): Tsukamura,M.;Mizuno,S.;Tsukamura,S.
Journal: Int. J. Syst. Bacteriol.
Title: Numerical analysis of rapidly growing scotochromogenic mycobacteria, including Mycobacterium obusense sp. nov., nom. rev., Mycobacterium rhodesiae sp. nov., nom. rev., Mycobacterium aichiense sp. nov., nom. rev., Mycobacterium chubuense sp. nov., nom. re
Volume: 31
Page(s): 263-275
Year: 1981
Ref #: 6510
Journal: Jap. J. Microbiol.
Volume: 15
Page(s): 407
Year: 1971
Data: (ATCC 27024) Type strain / M. Tsukamura, Japan in 1971 / L. King, Rhodesia / Sputum / Tsukamura, M. et al. (1971) Jap. J. Microbiol. 15, 407
Accession Date: 01/01/1971
Authority: Tsukamura 1981
Depositor: TSUKAMURA M
Taxonomy: TaxLink: S1975 (Mycobacterium rhodesiae Tsukamura 1981) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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