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Bacteria and Mycoplasmas detail

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Bacteria Collection: Gordonia rubropertincta

NCTC Number: NCTC 10668
Current Name: Gordonia rubropertincta
Original Strain Reference: 3605
Other Collection No: ATCC 25593; CIP 100848; DSM 43248; IMET 7372
Previous Catalogue Name: Rhodococcus corallinus
Type Strain: Yes
Family: Nocardiaceae
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1324131
16S rRNA Gene Sequence: >gb|AY995557|DSM 43248|Gordonia rubripertincta strain DSM 43248 16S ribosomal RNA gene,partial sequence.| tcaggacgaacgctg...
Miscellaneous Sequence Data: >gb|AY972062|DSM 43248|Gordonia rubripertincta strain DSM 43248 DNA gyrase subunit B(gyrB) gene, partial cds.| gcggcggcaagttcg...
Extended Bibliography: showhide Show bibliography
Ref #: 95449
Author(s): Shen,F.T.;Lu,H.L.;Lin,J.L.;Huang,W.S.;Arun,A.B.;Young,C.C.
Journal: Res Microbiol
Title: Phylogenetic analysis of members of the metabolically diverse genus Gordonia based on proteins encoding the gyrB gene
Volume: 157
Page(s): 367-75
Year: 2006
Keyword(s): Amino Acid Sequence DNA Gyrase/genetics/*metabolism DNA Primers Gordonia Bacterium/*classification/enzymology Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/genetics Sequence Homology, Amino Acid
Remarks: Members of the metabolically diverse genus Gordonia were isolated from various biotopes including pristine and polluted sites around Taiwan. Identification, comparison and diversity assessment based on the gyrB gene were carried out using a newly developed primer pair for gyrB. The 16S rRNA gene was also sequenced for comparison. A 1.2-kb fragment of the gyrB gene of 17 Gordonia strains including type strains was determined by direct sequencing of PCR amplified fragments. A total of 25 strains (8 of which were retrieved from a public database) of the genus Gordonia form a distinct phyletic line in the GyrB-based tree and are separated from other closely related species of genera of the suborder Corynebacterineae. Sequence similarity of the gyrB sequence from twelve Gordonia type strains ranged from 79.3 to 97.2%, corresponding to between 270 and 41 nucleotide differences, while there was only a 0.3-3.8% difference in 16S rRNA gene sequence similarity at the interspecies level. Phylogenetic analysis based on the GyrB sequence deduced from the gyrB gene is consistent with that of DNA-DNA hybridization results and provides a better discrimination within the species of Gordonia compared to the 16S rRNA gene. The present study demonstrates that gyrB gene analysis will aid in describing novel species belonging to the genus Gordonia.
URL: 16310344
Ref #: 2184
Author(s): Goodfellow,M.;Alderson,G.
Journal: J. Gen. Microbiol.
Title: The actinomycete-genus Rhodococcus: a home for the "rhodochrous" complex.
Volume: 100
Page(s): 99-122
Year: 1977
Data: (ATCC 25593) Type strain / M. Tsukamura, Obu, Chita, Aichi-ken, Japan in 1970 / Soil in 1967
Accession Date: 04/02/2003
Authority: (Hefferan 1904) Stackebrandt et al. 1989
Taxonomy: TaxLink: ? (NCTC 10668) - Date of change: 04/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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