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Bacteria Collection: Staphylococcus chromogenes

NCTC Number: NCTC 10530
Current Name: Staphylococcus chromogenes
Original Strain Reference: 1462
Other Collection No: ATCC 43764; CCM 3387; DSM 20454; 1462
Previous Catalogue Name: Staphylococcus hyicus subp. Chromogenes
Type Strain: Yes
Family: Staphylococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: mammal, pig skin
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS825164
16S rRNA Gene Sequence: >gb|D83360|MAFF911474| ATCC43764T|Staphylococcus chromogenes (strain MAFF911474, ATCC43764T) gene for16S rRNA, partial sequence.| aggatgaacgctggc... >gb|AY688044|DSM 20454|Staphylococcus chromogenes strain DSM 20454 16S ribosomal RNA gene,partial sequence.| catgcaagtcgagcg... >gb|U39770|ATCC 43764|Staphylococcus chromogenes 16S-23S ribosomal RNA spacer region.| aaggataatatacgg...
23S rRNA Gene Sequence: >gb|U39770|ATCC 43764|Staphylococcus chromogenes 16S-23S ribosomal RNA spacer region.| aaggataatatacgg...
Extended Bibliography: showhide Show bibliography
Ref #: 81079
Author(s): Forsman,P.;Tilsala-Timisjarvi,A.;Alatossava,T.
Journal: Microbiology
Title: Identification of staphylococcal and streptococcal causes of bovine mastitis using 16S-23S rRNA spacer regions
Volume: 143 ( Pt 11)
Page(s): 3491-500
Year: 1998
Keyword(s): GENBANK/U39765 GENBANK/U39766 GENBANK/U39767 GENBANK/U39768 GENBANK/U39769 GENBANK/U39770 GENBANK/U39771 GENBANK/U39772 GENBANK/U39773 GENBANK/U39813 GENBANK/U39814 GENBANK/U90010 GENBANK/U90011 GENBANK/U90012 GENBANK/U90013 GENBANK/U90014 GENBANK/U90015 GENBANK/U90016 GENBANK/U90017 GENBANK/U90018 GENBANK/U90019 GENBANK/U90020 GENBANK/U90021 GENBANK/U90022 GENBANK/U90023 GENBANK/U90024 GENBANK/U90025 GENBANK/U90026 GENBANK/U90027 Animals Base Sequence Cattle DNA Primers DNA, Bacterial/*genetics DNA, Ribosomal/*genetics Female Mastitis, Bovine/*microbiology Milk/microbiology Molecular Sequence Data Polymerase Chain Reaction/methods RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Sequence Alignment Sequence Analysis, DNA Sequence Homology, Nucleic Acid Species Specificity Staphylococcus/*genetics Streptococcus/*genetics Variation (Genetics)/genetics
Remarks: Bovine mastitis is caused mainly by certain Staphylococcus and Streptococcus species. The sequences of the 16S-23S rRNA spacer regions were determined for the nine species which cause mastitis: Staphylococcus aureus, Staphylococcus chromogenes, Staphylococcus epidermidis, Staphylococcus hyicus, Staphylococcus simulans, Staphylococcus xylosus, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis. Significant variation was found between the spacer sequences of different species with the lengths of the spacers varying from 240 to 461 bp. Between genera the spacers shared only short conserved regions (8-9 bp) and within genera the sequence identities varied from 53 to 85%. This variation made it possible to construct specific primer pairs for these species and genera. The specificities of these primers were tested with 25 bacterial species and 51 isolates from cattle with clinical mastitis. The DNA-based identification of the mastitis species was mostly successful.
URL: 9387227
Ref #: 81199
Author(s): Becker,K.;Harmsen,D.;Mellmann,A.;Meier,C.;Schumann,P.;Peters,G.;von Eiff,C.
Journal: J Clin Microbiol
Title: Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species
Volume: 42
Page(s): 4988-95
Year: 2004
Keyword(s): GENBANK/AY688029 GENBANK/AY688030 GENBANK/AY688031 GENBANK/AY688032 GENBANK/AY688033 GENBANK/AY688034 GENBANK/AY688035 GENBANK/AY688036 GENBANK/AY688037 GENBANK/AY688038 GENBANK/AY688039 GENBANK/AY688040 GENBANK/AY688041 GENBANK/AY688042 GENBANK/AY688043 GENBANK/AY688044 GENBANK/AY688045 GENBANK/AY688046 GENBANK/AY688047 GENBANK/AY688048 GENBANK/AY688049 GENBANK/AY688050 GENBANK/AY688051 GENBANK/AY688052 GENBANK/AY688053 GENBANK/AY688054 GENBANK/AY688055 GENBANK/AY688056 GENBANK/AY688057 GENBANK/AY688058 GENBANK/AY688059 GENBANK/AY688060 GENBANK/AY688061 GENBANK/AY688062 GENBANK/AY688063 GENBANK/AY688064 GENBANK/AY688065 GENBANK/AY688066 GENBANK/AY688067 GENBANK/AY688068 GENBANK/AY688069 GENBANK/AY688070 GENBANK/AY688071 GENBANK/AY688072 GENBANK/AY688073 GENBANK/AY688074 GENBANK/AY688075 GENBANK/AY688076 GENBANK/AY688077 GENBANK/AY688078 GENBANK/AY688079 GENBANK/AY688080 GENBANK/AY688081 GENBANK/AY688082 GENBANK/AY688083 GENBANK/AY688084 GENBANK/AY688085 GENBANK/AY688086 GENBANK/AY688087 GENBANK/AY688088 GENBANK/AY688089 GENBANK/AY688090 GENBANK/AY688091 GENBANK/AY688092 GENBANK/AY688093 GENBANK/AY688094 GENBANK/AY688095 GENBANK/AY688096 GENBANK/AY688097 GENBANK/AY688098 GENBANK/AY688099 GENBANK/AY688100 GENBANK/AY688101 GENBANK/AY688102 GENBANK/AY688103 GENBANK/AY688104 GENBANK/AY688105 GENBANK/AY688106 GENBANK/AY688107 GENBANK/AY688108 GENBANK/AY688109 *Bacterial Typing Techniques DNA, Bacterial/analysis DNA, Ribosomal/analysis *Databases, Nucleic Acid Genes, rRNA Humans Molecular Sequence Data RNA, Ribosomal, 16S/*genetics Sequence Analysis, DNA Species Specificity Staphylococcal Infections/*microbiology Staphylococcus/*classification/genetics/metabolism
Remarks: To establish an improved ribosomal gene sequence database as part of the Ribosomal Differentiation of Microorganisms (RIDOM) project and to overcome the drawbacks of phenotypic identification systems and publicly accessible sequence databases, both strands of the 5' end of the 16S ribosomal DNA (rDNA) of 81 type and reference strains comprising all validly described staphylococcal (sub)species were sequenced. Assuming a normal distribution for pairwise distances of all unique staphylococcal sequences and choosing a reporting criterion of > or =98.7% similarity for a "distinct species," a statistical error probability of 1.0% was calculated. To evaluate this database, a 16S rDNA fragment (corresponding to Escherichia coli positions 54 to 510) of 55 clinical Staphylococcus isolates (including those of the small-colony variant phenotype) were sequenced and analyzed by the RIDOM approach. Of these isolates, 54 (98.2%) had a similarity score above the proposed threshold using RIDOM; 48 (87.3%) of the sequences gave a perfect match, whereas 83.6% were found by searching National Center for Biotechnology Information (NCBI) database entries. In contrast to RIDOM, which showed four ambiguities at the species level (mainly concerning Staphylococcus intermedius versus Staphylococcus delphini), the NCBI database search yielded 18 taxon-related ambiguities and showed numerous matches exhibiting redundant or unspecified entries. Comparing molecular results with those of biochemical procedures, ID 32 Staph (bioMerieux, Marcy I'Etoile, France) and VITEK 2 (bioMerieux) failed to identify 13 (23.6%) and 19 (34.5%) isolates, respectively, due to incorrect identification and/or categorization below acceptable values. In contrast to phenotypic methods and the NCBI database, the novel high-quality RIDOM sequence database provides excellent identification of staphylococci, including rarely isolated species and phenotypic variants.
URL: 15528685
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 1331
Author(s): Devriese,L.A.;Hajek,V.;Oeding,P.;Meyer,S.A.;Schleifer,K.H.
Journal: Int. J. Syst. Bacteriol.
Title: Staphylococcus hyicus (Sompolinsky 1953) comb. nov. and Staphylococcus hyicus subsp. chromogenes subsp. nov.
Volume: 28
Page(s): 482-490
Year: 1978
Ref #: 1334
Author(s): Schleifer,K.H.;Meyer,S.A.;Rupprecht,M.
Journal: Arch. Microbiol.
Title: Relatedness among coagulase-negative staphylococci: deoxyribonucleic acid reassociation and comparative immunological studies.
Volume: 122
Page(s): 93-101
Year: 1979
Ref #: 3018
Journal: Int. J. Syst. Bacteriol.
Title: Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 23.
Volume: 37
Page(s): 179-180
Year: 1987
Ref #: 3339
Author(s): Hajek,V.;Devriese,L.A.;Mordarski,M.;Goodfellow,M.;Pulverer,G.
Journal: System. Appl. Microbiol.
Title: Elevation of Staphylococcus hyicus subsp. chromogenes (Devriese et al., 1978) to species status: Staphylococcus chromogenes (Devriese et al., 1978) comb. nov.
Volume: 8
Page(s): 169-173
Year: 1986
Data: Type strain / A. C. Baird-Parker, Sharnbrook, Bedford in 1967 / Pia skin
Accession Date: 01/01/1967
History: RECEIVED FROM BAIRD-PARKER A C UNILEVER RESEARCH BEDFORD PRE:FR
Authority: (Devriese et al. 1978) Hajek et al. 1987
Depositor: BAIRD-PARKER A C
Taxonomy: TaxLink: S2789 (Staphylococcus hyicus (Sompolinski 1953) Devriese et al. 1978) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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