Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Mycobacterium chitae

NCTC Number: NCTC 10485
Current Name: Mycobacterium chitae
Original Strain Reference: 2502
Other Collection No: ATCC 19627; 2502
Previous Catalogue Name: Mycobacterium chitae
Type Strain: Yes
Family: Mycobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48-72 hours, 37°C, aerobic
Conditions for growth on liquid media: glucose broth,37, aerobic
Isolated From: soil
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1275660
16S rRNA Gene Sequence: >gb|U92090|ATCC14472|Mycobacterium chelonae ATCC 14472, 16S ribosomal RNA gene, partialsequence.| atcccatctctaggt... >gb|U92090|ATCC14472|Mycobacterium [chelonae] ATCC 14472 16S ribosomal RNA gene, partialsequence.| atcccatctctaggt...
Bibliography: TSUKAMURA M 1967 JAP J MICROBIOL 11 43
Extended Bibliography: showhide Show bibliography
Ref #: 95444
Author(s): Talaat,A.M.;Reimschuessel,R.;Trucksis,M.
Journal: Vet Microbiol
Title: Identification of mycobacteria infecting fish to the species level using polymerase chain reaction and restriction enzyme analysis
Volume: 58
Page(s): 229-37
Year: 1998
Keyword(s): GENBANK/U92088 GENBANK/U92089 GENBANK/U92090 Animals Base Sequence Conserved Sequence DNA Primers Deoxyribonuclease BamHI Deoxyribonucleases, Type II Site-Specific *Fish Diseases Fishes Humans Mycobacterium/*classification/genetics/*isolation & purification Mycobacterium Infections/diagnosis/*veterinary Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics Restriction Mapping/*methods
Remarks: An assay is described utilizing PCR technology for a rapid diagnostic test to identify fish infection with Mycobacterium marinum, M. fortuitum and M. chelonae. A 924 bp DNA fragment from a highly conserved area of the mycobacterial 16S rRNA gene was amplified using mycobacteria genus-specific primers and digested with restriction enzymes (BanI and ApaI). This examination yielded unique restriction patterns for each mycobacterial specie enabling identification of mycobacteria infecting fish to the species level. The protocol can be applied to purified DNA, a simple colony preparation or infected fish tissue. This protocol can be completed in 1-2 days.
URL: 9453133
Ref #: 13165
Author(s): Talaat,A.M.;Reimschuessel,R.;Trucksis,M.
Journal: Vet Microbiol
Title: Identification of mycobacteria infecting fish to the species level using polymerase chain reaction and restriction enzyme analysis
Volume: 58
Page(s): 229-37
Year: 1998
Keyword(s): GENBANK/U92088 GENBANK/U92089 GENBANK/U92090 Animal Base Sequence Conserved Sequence DNA Primers Deoxyribonuclease BamHI Deoxyribonucleases, Type II Site-Specific *Fish Diseases Fishes Human Mycobacterium/*classification/genetics/*isolation & purification Mycobacterium Infections/diagnosis/*veterinary Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics Restriction Mapping/*methods Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S.
Remarks: An assay is described utilizing PCR technology for a rapid diagnostic test to identify fish infection with Mycobacterium marinum, M. fortuitum and M. chelonae. A 924 bp DNA fragment from a highly conserved area of the mycobacterial 16S rRNA gene was amplified using mycobacteria genus-specific primers and digested with restriction enzymes (BanI and ApaI). This examination yielded unique restriction patterns for each mycobacterial specie enabling identification of mycobacteria infecting fish to the species level. The protocol can be applied to purified DNA, a simple colony preparation or infected fish tissue. This protocol can be completed in 1-2 days.
URL: 98115223
Data: (ATCC 19627) Type strain / M. Tsukamura, Obu, Chita, Aichi-ken, Japan in 1966 / Soil, Japan / Tsukamura, M. (1967) Jap. J. Microbiol. 11, 43
Accession Date: 01/01/1966
History: TSUKAMURA M,NATIONAL SANATORIUM,CHUBU CHEST HOSP,OBU,AICHI PRE:FR
Authority: Tsukamura 1967 (AL)
Depositor: TSUKAMURA M
Taxonomy: TaxLink: S1943 (Mycobacterium chitae Tsukamura 1967) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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Ampoule (Bacteria)

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