Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Bacillus subtilis subsp spizizenii

NCTC Number: NCTC 10400
Current Name: Bacillus subtilis subsp spizizenii
Original Strain Reference: PCI 219
Other Collection No: ATCC 6633; BUCSAV 425 LOC:UNITED; CCM 1999; DSM 347; HANKEY B 14; IAM 1069; NCIB 8054; NR SMITH 231; WDCM 00003
Previous Catalogue Name: Bacillus subtilis
Type Strain: No
Family: Bacillaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
16S rRNA Gene Sequence: >gb|AF200210|ATCC 6633|Bacillus subtilis strain ATCC 6633 16S ribosomal RNA gene, partialsequence.| ccgaagttatcatac... >gb|AB018486|ATCC 6633|Bacillus subtilis gene for 16S rRNA, partial sequence, strain:ATCC6633.| aggacgaacgctggc... >gb|AY616162|ATCC 6633|Bacillus subtilis strain ATCC 6633 16S ribosomal RNA gene, partialsequence.| catagggaatcttcc... >gb|DQ207730|CCM 1999| ATCC 6633|Bacillus subtilis strain CCM 1999 16S ribosomal RNA gene, completesequence.| agagtttgatcctgg...
Bibliography: 1973 BRIT PHARMACOPOEIA 104
Extended Bibliography: showhide Show bibliography
Ref #: 95513
Author(s): Sipos,R.;Szekely,A.J.;Palatinszky,M.;Revesz,S.;Marialigeti,K.;Nikolausz,M.
Journal: FEMS Microbiol Ecol
Title: Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis
Volume: 60
Page(s): 341-50
Year: 2007
Remarks: In the attempt to explore complex bacterial communities of environmental samples, primers hybridizing to phylogenetically highly conserved regions of 16S rRNA genes are widely used, but differential amplification is a recognized problem. The biases associated with preferential amplification of multitemplate PCR were investigated using 'universal' bacteria-specific primers, focusing on the effect of primer mismatch, annealing temperature and PCR cycle number. The distortion of the template-to-product ratio was measured using predefined template mixtures and environmental samples by terminal restriction fragment length polymorphism analysis. When a 1 : 1 genomic DNA template mixture of two strains was used, primer mismatches inherent in the 63F primer presented a serious bias, showing preferential amplification of the template containing the perfectly matching sequence. The extent of the preferential amplification showed an almost exponential relation with increasing annealing temperature from 47 to 61 degrees C. No negative effect of the various annealing temperatures was observed with the 27F primer, with no mismatches with the target sequences. The number of PCR cycles had little influence on the template-to-product ratios. As a result of additional tests on environmental samples, the use of a low annealing temperature is recommended in order to significantly reduce preferential amplification while maintaining the specificity of PCR.
URL: 17343679
Ref #: 95469
Author(s): Mamane-Gravetz,H.;Linden,K.G.
Journal: J Appl Microbiol
Title: Relationship between physiochemical properties, aggregation and u.v. inactivation of isolated indigenous spores in water
Volume: 98
Page(s): 351-63
Year: 2005
Keyword(s): *Bacillus Disinfection/methods Hydrophobicity Particle Size Spores Ultraviolet Rays *Water Microbiology *Water Purification
Remarks: AIMS: The objective of the study was to compare ultraviolet (u.v.) inactivation kinetics of indigenous aerobic spores in surface water with their laboratory-cultured spore isolates and to investigate the relationship between physicochemical characteristics and u.v. inactivation kinetics of spore isolates. METHODS AND RESULTS: Lake water samples were analysed for the presence of indigenous aerobic spores. Different bacterial isolates from the heterogeneous indigenous population were genetically characterized, resporulated and examined for hydrophobicity, surface charge, particle size distribution and survival at different u.v. 254 nm fluence levels. Cultured isolated spores exhibited a three-stage inactivation curve consisting of shoulder, first order and tailing regions whereas indigenous spores exhibited only one stage of linear kinetics. Hydrophobicity of the Bacillus spore isolates was inversely related to the extent of u.v. inactivation before tailing occurred. CONCLUSIONS: Tailing in the u.v. inactivation curves results from aggregation of a portion of the spore population because of hydrophobic interactions, supporting the link between aggregation of spores, hydrophobicity and u.v. inactivation. SIGNIFICANCE AND IMPACT OF THE STUDY: Evidence of the link between spore physicochemical parameters and u.v. disinfection performance furthers the understanding of factors that affect inactivation of microbes in natural waters supplied to drinking water treatment plants.
URL: 15659190
Ref #: 101
Author(s): Dubnau,D.;Smith,I.;Morrel,P.;Marmur,J.
Journal: Proc. Natl. Acad. Sci. U.S.A.
Title: Gene conservation in Bacillus species. I. Conserved genetic and nucleic base sequence homologies.
Volume: 54
Page(s): 491-498
Year: 1965
Ref #: 2183
Title: European Pharmacopeia. Test for sterility. Revised Text. July
Year: 1978
Ref #: 2358
Author(s): Roberts,R.J.
Journal: Nucl. Acids Res.
Title: Restriction and modification enzymes and their recognition sequences.
Volume: 13,
Page(s): r165-r200
Year: 1985
Ref #: 5998
Author(s): FachnormenausschußMaterialprüfung(FNM)desDeutschenInstitutsfürNormungDIN
Title: DIN 54 380. Prüfung auf Zusätze von antimikrobiellen Bestandteilen. Prüfung von Papier, Karton und Pappe.
Year: 1978
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Ref #: 7330
Author(s): Heinzmann,S.;Entian,K.-D.
Title: Nisin immunity in Bacillus subtilis. Poster No. PA016 at VAAM-Meeting, Frankfurt.
Year: 1998
Ref #: 7331
Author(s): Stein,T.H.;Entian,K.-D.
Title: Characterization of peptide antibiotics produced by Bacillus subtilis ATCC 6633. Poster No. PA019 at VAAM-Meeting, Frankfurt.
Year: 1998
Ref #: 737
Title: Handbook of Microbiology Vol.III. Microbial products.
Year: 1973
Ref #: 738
Title: Europäisches Arzneibuch, Band II, amtliche Ausgabe.
Year: 1975
Ref #: 739
Author(s): Tittiger,F.;Kingscote,B.;Meldrum,B.;Prior,M.
Journal: Can. J. Comp. Med.
Title: Survey of antibiotic residues in Canadian slaughter animals.
Volume: 39
Page(s): 178-182
Year: 1975
Ref #: 7413
Author(s): Stein,T.;Düsterhus,S.;Stroh,A.;Entian,K.-D.
Title: Identification and characterization of the structural gene sbo for, and the chemical properties of, subtilosin A, a bacteriocine produced by Bacillus subtilis ATCC 6633. Poster No. K-D 10 at VAAM-Conference, Göttingen.
Year: 1999
Ref #: 98
Author(s): Lovett,P.S.;Young,F.E.
Journal: J. Bacteriol.
Title: Identification of Bacillus subtilis NRRL B-3275 as a strain of Bacillus pumilus.
Volume: 100
Page(s): 658-661
Year: 1969
Data: (N. R. Smith 231, Hankey B 14, BUCSAV 425, ATCC 6633, NCIB 8054) NCIB in 1965 / ATCC / Assay of paromomycin sulphate, chlortetracycline, streptomycin viomycin and penicillin in milk / Produces subtilin / British Pharmacopoeia 1973, p. A104
Accession Date: 01/01/1965
History: ATCC
Authority: (Ehrenberg 1835) Cohn 1872 (AL)
Depositor: NCIB
Taxonomy: TaxLink: S436 (Bacillus subtilis subsp. subtilis (Ehrenberg 1835) Cohn 1872) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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