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Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Pseudomonas alcaligenes

NCTC Number: NCTC 10367
Current Name: Pseudomonas alcaligenes
Original Strain Reference: RH 1577
Other Collection No: ATCC 14909; DSM 50342; RH 1577
Previous Catalogue Name: Pseudomonas alcaligenes
Type Strain: Yes
Family: Pseudomonadaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, aerobic
Isolated From: hot water;isolated from swimming pool
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1143227
16S rRNA Gene Sequence: >gb|AF094721|ATCC 14909|Pseudomonas alcaligenes 16S ribosomal RNA gene, partial sequence.| catgaatcactccgt...
Bibliography: HUGH R & IKARI P 1964 INT.BULL.BACT.NOMEN. TAXON. 14 103; IKARI P & HUGH R
Extended Bibliography: showhide Show bibliography
Ref #: 95468
Author(s): Petri,R.;Imhoff,J.F.
Journal: Syst Appl Microbiol
Title: The relationship of nitrate reducing bacteria on the basis of narH gene sequences and comparison of narH and 16S rDNA based phylogeny
Volume: 23
Page(s): 47-57
Year: 2000
Keyword(s): GENBANK/AJ288121 GENBANK/AJ288122 GENBANK/AJ288123 GENBANK/AJ288124 GENBANK/AJ288125 GENBANK/AJ288126 GENBANK/AJ288127 GENBANK/AJ288128 GENBANK/AJ288129 GENBANK/AJ288130 GENBANK/AJ288131 GENBANK/AJ288132 GENBANK/AJ288133 GENBANK/AJ288134 GENBANK/AJ288135 GENBANK/AJ288136 GENBANK/AJ288137 GENBANK/AJ288138 GENBANK/AJ288139 GENBANK/AJ288140 GENBANK/AJ288141 GENBANK/AJ288142 GENBANK/AJ288143 GENBANK/AJ288144 GENBANK/AJ288145 GENBANK/AJ288146 GENBANK/AJ288147 GENBANK/AJ288148 GENBANK/AJ288149 GENBANK/AJ288150 etc. Bacteria/classification/*genetics/metabolism DNA, Bacterial/analysis/genetics DNA, Ribosomal/analysis/genetics Molecular Sequence Data Nitrate Reductase Nitrate Reductases/*genetics/metabolism Nitrates/*metabolism Oxidation-Reduction *Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics Sequence Analysis, DNA
Remarks: On the basis of available nitrate reductase gene sequences primer pairs were designed to specifically amplify gene stretches of the beta-subunit of the membrane-bound nitrate reductase (narH). Additional sequences of this gene were amplified and sequenced from pure cultures of reference species and new isolates. The distribution and phylogeny of this gene in denitrifying and nitrate-reducing bacteria was analysed. Comparison of phylogenetic trees based on 16S rDNA sequences with those based on narH sequences revealed highly similar relationships of both genes from most of the bacteria analysed. Since highly conserved functional cysteine clusters within bacterial and archaeal narH sequences support a linear evolution from one common progenitor a long evolutionary history of the respiratory membrane-bound nitrate reductase can be inferred from our phylogenetic data.
URL: 10879978
Ref #: 72258
Author(s): Lorenz,M.G.;Sikorski,J.
Journal: Microbiology
Title: The potential for intraspecific horizontal gene exchange by natural genetic transformation: sexual isolation among genomovars of Pseudomonas stutzeri
Volume: 146 Pt 12
Page(s): 3081-90
Year: 2000
Keyword(s): GENBANK/AJ279953 GENBANK/AJ279954 GENBANK/AJ279955 GENBANK/AJ279956 GENBANK/AJ279957 GENBANK/AJ279958 GENBANK/AJ279959 GENBANK/AJ279960 GENBANK/AJ279961 GENBANK/AJ279962 GENBANK/AJ279963 GENBANK/AJ279964 GENBANK/AJ279965 GENBANK/AJ279966 GENBANK/AJ279967 *Conjugation, Genetic/genetics DNA, Bacterial/analysis/genetics DNA-Directed RNA Polymerases/*genetics *Gene Transfer, Horizontal Genotype Humans Molecular Sequence Data Phylogeny Pseudomonas/classification/*genetics/physiology Sequence Analysis, DNA *Transformation, Bacterial
Remarks: The potential for natural genetic transformation among the seven genomovars (gvs) of Pseudomonas stutzeri was investigated. Of the 12 strains originating from a variety of environments, six strains (50%) from five gvs were competent for DNA uptake (Rif(R) marker). The transformation frequencies varied over more than three orders of magnitude. With three highly transformable strains (ATCC 17587, ATCC 17641, JM300) from two gvs and all other strains as DNA donors, sexual isolation from other pseudomonad species (Pseudomonas alcaligenes, Pseudomonas mendocina) and also from other P. stutzeri gvs was observed (i.e. heterogamic transformation was reduced). For ATCC 17587 (gv 2) and ATCC 17641 (gv 8), heterogamic transformation was up to two and three orders of magnitude lower with other P. stutzeri gv and the other species employed, respectively, than in homogamic transformations. Interestingly, whereas with ATCC 17587 and ATCC 17641 heterogamic transformation with donors of the same gv was as high as homogamic transformation, JM300 (gv 8) was sexually isolated from its nearest relative (ATCC 17641). Also, sexual isolation of JM300 from other P. stutzeri gvs was most pronounced among the recipients tested, in some cases reaching the highest levels found with the other species as DNA donors (reduction of heterogamic transformation by 4000-fold). Results obtained here from nucleotide sequence analysis of part (422 nt) of the gene for the RNA polymerase ss subunit (rpoB) from various strains indicated that sexual isolation of ATCC 17641 increased with nucleotide sequence divergence. Implications of the observed great heterogeneity in transformability, competence levels and sexual isolation among strains are discussed with regard to the evolution of P. stutzeri.
URL: 11101666
Ref #: 13358
Author(s): Petri,R.;Imhoff,J.F.
Journal: Syst Appl Microbiol
Title: The relationship of nitrate reducing bacteria on the basis of narH gene sequences and comparison of narH and 16S rDNA based phylogeny
Volume: 23
Page(s): 47-57
Year: 2000
Keyword(s): GENBANK/AJ288121 GENBANK/AJ288122 GENBANK/AJ288123 GENBANK/AJ288124 GENBANK/AJ288125 GENBANK/AJ288126 GENBANK/AJ288127 GENBANK/AJ288128 GENBANK/AJ288129 GENBANK/AJ288130 GENBANK/AJ288131 GENBANK/AJ288132 GENBANK/AJ288133 GENBANK/AJ288134 GENBANK/AJ288135 GENBANK/AJ288136 GENBANK/AJ288137 GENBANK/AJ288138 GENBANK/AJ288139 GENBANK/AJ288140 GENBANK/AJ288141 GENBANK/AJ288142 GENBANK/AJ288143 GENBANK/AJ288144 GENBANK/AJ288145 GENBANK/AJ288146 GENBANK/AJ288147 GENBANK/AJ288148 GENBANK/AJ288149 GENBANK/AJ288150 etc. Bacteria/classification/*genetics/metabolism DNA, Bacterial/analysis/genetics DNA, Ribosomal/analysis/genetics Molecular Sequence Data Nitrate Reductases/*genetics/metabolism Nitrates/*metabolism Oxidation-Reduction *Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics Sequence Analysis, DNA Support, Non-U.S. Gov't
Remarks: On the basis of available nitrate reductase gene sequences primer pairs were designed to specifically amplify gene stretches of the beta-subunit of the membrane-bound nitrate reductase (narH). Additional sequences of this gene were amplified and sequenced from pure cultures of reference species and new isolates. The distribution and phylogeny of this gene in denitrifying and nitrate-reducing bacteria was analysed. Comparison of phylogenetic trees based on 16S rDNA sequences with those based on narH sequences revealed highly similar relationships of both genes from most of the bacteria analysed. Since highly conserved functional cysteine clusters within bacterial and archaeal narH sequences support a linear evolution from one common progenitor a long evolutionary history of the respiratory membrane-bound nitrate reductase can be inferred from our phylogenetic data.
URL: 20336325
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Data: (ATCC 14909) Type strain / ATCC in 1964 / Swimming pool in 1961 / Hugh, R. & Ikari, P. (1964) Int. Bull. bact. Nomencl.Taxon. 14, 103
Accession Date: 01/01/1964
History: ISOLATED BY HUGH R ,G.WASHINGTON U.,WASHINGTON D.C.
Authority: Monias 1928 (AL)
Depositor: ATCC
Taxonomy: TaxLink: S2404 (Pseudomonas alcaligenes Monias 1928) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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