Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Geobacillus stearothermophilus

NCTC Number: NCTC 10339
Current Name: Geobacillus stearothermophilus
Original Strain Reference: Donk
Other Collection No: ATCC 12980; CCM 2062; CCUG 26241; DONK; DSM 22; IAM 11062; IFO 12550; N R SMITH T 18; NCA 26; NCIB 8923
Previous Catalogue Name: Bacillus stearothermophilus
Type Strain: Yes
Family: Bacillaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: nutrient agar,60, facultative anaerobe
Conditions for growth on liquid media: nutrient broth,60, facultative anaerobe
16S rRNA Gene Sequence: >gb|AF478064|ATCC 12980|Geobacillus stearothermophilus clone EM-2_F 16S ribosomal RNA gene,partial sequence; 16S-23S internal transcribed spacer, completesequence; and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata... >gb|AJ294817|DSM 22T|Bacillus stearothermophilus 16S rRNA gene, strain DSM 22T.| aacgctggcggcgtg... >gb|AB021196|IFO12550|Bacillus stearothermophilus gene for 16S ribosomal RNA.| acgaacgctggcggc...
23S rRNA Gene Sequence: >gb|AF478064|ATCC 12980|Geobacillus stearothermophilus clone EM-2_F 16S ribosomal RNA gene,partial sequence; 16S-23S internal transcribed spacer, completesequence; and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata...
Bibliography: SMITH N R ET AL 1964 J GEN MICROBIOL 34 269
Extended Bibliography: showhide Show bibliography
Ref #: 95498
Author(s): Goto,K.;Omura,T.;Hara,Y.;Sadaie,Y.
Journal: J Gen Appl Microbiol
Title: Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus
Volume: 46
Page(s): 1-8
Year: 2002
Remarks: A comparison of 16S rRNA gene (rDNA) sequences was made among type strains of 69 Bacillus species approved in the International Journal of Systematic Bacteriology (IJSB) until 1998. The results suggested that 5' end region (approx. 275 bp) was the hypervariant region (HV region) in the gene and was highly specific for each type strain. Furthermore, a sequence analysis of the HV region of Bacillus strains revealed that this region was highly conserved within the species. These results indicate that the HV region is a useful index for the identification or grouping of Bacillus species.
URL: 12483598
Ref #: 22093
Author(s): Xu,D.;Cote,J.C.
Journal: Int J Syst Evol Microbiol
Title: Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3' end 16S rDNA and 5' end 16S-23S ITS nucleotide sequences
Volume: 53
Page(s): 695-704
Year: 2003
Keyword(s): GENBANK/AF478062 GENBANK/AF478063 GENBANK/AF478064 GENBANK/AF478065 GENBANK/AF478066 GENBANK/AF478067 GENBANK/AF478068 GENBANK/AF478069 GENBANK/AF478070 GENBANK/AF478071 GENBANK/AF478072 GENBANK/AF478073 GENBANK/AF478074 GENBANK/AF478075 GENBANK/AF478076 GENBANK/AF478077 GENBANK/AF478078 GENBANK/AF478079 GENBANK/AF478080 GENBANK/AF478081 GENBANK/AF478082 GENBANK/AF478083 GENBANK/AF478084 GENBANK/AF478085 GENBANK/AF478086 GENBANK/AF478087 GENBANK/AF478088 GENBANK/AF478089 GENBANK/AF478090 GENBANK/AF478091 GENBANK/AF478092 GENBANK/AF478093 GENBANK/AF478094 GENBANK/AF478095 GENBANK/AF478096 GENBANK/AF478097 GENBANK/AF478098 GENBANK/AF478099 GENBANK/AF478100 GENBANK/AF478101 GENBANK/AF478102 GENBANK/AF478103 GENBANK/AF478104 GENBANK/AF478105 GENBANK/AF478106 GENBANK/AF478107 GENBANK/AF478108 GENBANK/AF478109 GENBANK/AF478110 GENBANK/AF478111 3' Untranslated Regions/*genetics 5' Untranslated Regions/*genetics Bacillus/*classification/genetics DNA, Ribosomal/analysis DNA, Ribosomal Spacer/*analysis Genes, rRNA Molecular Sequence Data *Phylogeny RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/genetics *Sequence Analysis, DNA Species Specificity
Remarks: The nucleotide sequences of the 3' end of the 16S rDNA and the 16S-23S internal transcribed spacer (ITS) of 40 Bacillaceae species were determined. These included 21 Bacillus, 9 Paenibacillus, 6 Brevibacillus, 2 Geobacillus, 1 Marinibacillus and 1 Virgibacillus species. Comparative sequence analysis of a 220 bp region covering a highly conserved 150 bp sequence located at the 3' end of the 16S rRNA coding region and a conserved 70 bp sequence located at the 5' end of the 16S-23S ITS of the 40 species and six sequences available in GenBank were used to infer the phylogenetic relationships between all 46 taxa. When a maximal distance (D(max), where D refers to the number of nucleotide substitutions per site) of 0.31 was introduced as a threshold to determine groupings, 10 phylogenetically distinct clusters were revealed. Twenty-six Bacillus species were separated in seven groups (I, II, III, IV, V, VI and X), but Bacillus circulans remained ungrouped. All six Brevibacillus species under study were in Group VII. The nine Paenibacillus species fell into two distinct groups (VIII and IX). Species with D(max) values within 0.05 were considered to be very closely related. These were Bacillus psychrophilus and Bacillus psychrosaccharolyticus in Group II; 'Bacillus maroccanus' and Bacillus simplex in Group II; Bacillus amyloliquefaciens, Bacillus atrophaeus, Bacillus mojavensis and Bacillus subtilis in Group VI; Bacillus fusiformis and Bacillus sphaericus in Group VI; Brevibacillus brevis and Brevibacillus formosus in Group VII; Paenibacillus gordonae and Paenibacillus validus in Group VIII; and Bacillus anthracis, Bacillus cereus, Bacillus mycoides and Bacillus thuringiensis in Group X. The phylogenetic classification presented here is, in general, in agreement with current classifications based on phenotypic and molecular data. Our findings suggest, however, that in some cases, further divisions or, conversely, further groupings might be warranted. Should current classifications be re-examined in the light of our results, D(max) values of 0.31 and 0.05, as exemplified here, may prove useful threshold values for the grouping of Bacillaceae into taxa akin to genera and species, respectively. These D(max) thresholds may also reveal, in a different way, bacterial species for which further characterization might be warranted for proper classification and/or reassignment.
URL: 12807189
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 2358
Author(s): Roberts,R.J.
Journal: Nucl. Acids Res.
Title: Restriction and modification enzymes and their recognition sequences.
Volume: 13,
Page(s): r165-r200
Year: 1985
Ref #: 3394
Author(s): Blumenstock,I.
Title: Bacillus coagulans Hammer 1915 und andere thermophile oder mesophile, säuretolerante Bacillus-Arten- eine taxonomische Untersuchung. Doctoral thesis.
Year: 1984
Ref #: 3396
Author(s): Nakamura,L.K.;Blumenstock,I.;Claus,D.
Journal: Int. J. Syst. Bacteriol.
Title: Taxonomic study of Bacillus coagulans Hammer 1915 with a proposal for Bacillus smithii sp. nov.
Volume: 38
Page(s): 63-73
Year: 1988
Ref #: 3768
Author(s): Fahmy,F.;Flossdorf,J.;Claus,D.
Journal: System. Appl. Microbiol.
Title: The DNA base composition of the type strains of the genus Bacillus.
Volume: 6
Page(s): 60-65
Year: 1985
Ref #: 4759
Journal: U. S. Pharmacopeia 22nd rev.
Page(s): 1488-1493
Year: 1990
Ref #: 6752
Author(s): Bartsch,K.;Schneider,R.;Schulz,A.
Journal: Appl. Environ. Microbiol.
Title: Stereospecific production of the herbicide phosphinotricin (glufosinate): purification of aspartate transaminase from Bacillus stearothermophilus, cloning of the corresponding gene, aspC, and application in a coupled transaminase process.
Volume: 62
Page(s): 3794-3799
Year: 1996
Ref #: 6848
Author(s): Spicher,G.;Peters,J.;Borchers,U.
Journal: Hyg. Med.
Title: Bacillus subtilis and Bacillus staerothermophilus as test organisms or bioindicators: the effect of carrier properties on the resistance to steam.
Volume: 3
Page(s): 190-195
Year: 1996
Ref #: 6904
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN EN 866-3. Biologische Systeme für die Prüfung von Sterilisatoren und Sterilisationsverfahren. Teil 3: Spezielle Systeme für den Gebrauch in Sterilisatoren mit feuchter Hitze.
Year: 1997
Ref #: 91
Author(s): Baker,H.;Frank,O.;Pasher,I.;Black,B.;Hutner,S.H.;Sobotka,H.
Journal: Can. J. Microbiol.
Title: Growth requirements of 94 strains of thermophilic bacilli.
Volume: 6
Page(s): 557-563
Year: 1960
Data: (N. R. Smith T 18, NCA 26, ATCC 12980, NCIB 8923) Type strain / T. Gibson, Edinburgh in 1963 / Smith, N. R. et al. (1964) J. gen. Microbiol. 34, 269
Accession Date: 01/01/1963
Authority: Donk 1920 (AL)
Depositor: GIBSON T
Taxonomy: TaxLink: S435 (Bacillus stearothermophilus) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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