NCTC Number: |
NCTC 10338
|
Current Name: |
Bacillus sphaericus
|
Original Strain Reference: |
Gibson 1013
|
Other Collection No: |
ATCC 14577; CCM 2120; DSM 28; GIBSON 1013; JCM 2502; MARBURG STRAIN WUND; NCIB 9370
|
Previous Catalogue Name: |
Bacillus sphaericus
|
Type Strain: |
Yes
|
Family: |
Bacillaceae
|
Hazard Group (ACDP): |
2
|
Release Restrictions: |
Terms & Conditions of Supply of Microbial Pathogens: Safety
|
Conditions for growth on solid media: |
Nutrient agar, 24-48 hours, 37°C, aerobic
|
Conditions for growth on liquid media: |
nutrient broth,37, facultative anaerobe
|
Whole Genome Sequence: |
http://www.ebi.ac.uk/ena/data/view/ERS1247818
|
16S rRNA Gene Sequence: |
>gb|AF478090|ATCC 14577|Bacillus sphaericus clone BSPH-1R 16S ribosomal RNA gene, partialsequence; 16S-23S internal transcribed spacer, complete sequence;and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata... >gb|AJ310084|DSM 28|Bacillus sphaericus 16S rRNA gene, strain DSM 28.| cctggctcaggacga... >gb|DQ286299|ATCC 14577|Bacillus sphaericus strain ATCC 14577 16S ribosomal RNA gene,partial sequence.| cataatgcagtcgag...
|
23S rRNA Gene Sequence: |
>gb|AF478090|ATCC 14577|Bacillus sphaericus clone BSPH-1R 16S ribosomal RNA gene, partialsequence; 16S-23S internal transcribed spacer, complete sequence;and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata...
|
Bibliography: |
NEIDE E 1904 ZENTBL BAKT PARASITK II ABT 12 350
|
Extended Bibliography: |
Show bibliography
Ref #: |
22093 |
Author(s): |
Xu,D.;Cote,J.C. |
Journal: |
Int J Syst Evol Microbiol |
Title: |
Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3' end 16S rDNA and 5' end 16S-23S ITS nucleotide sequences |
Volume: |
53 |
Page(s): |
695-704 |
Year: |
2003 |
Keyword(s): |
GENBANK/AF478062
GENBANK/AF478063
GENBANK/AF478064
GENBANK/AF478065
GENBANK/AF478066
GENBANK/AF478067
GENBANK/AF478068
GENBANK/AF478069
GENBANK/AF478070
GENBANK/AF478071
GENBANK/AF478072
GENBANK/AF478073
GENBANK/AF478074
GENBANK/AF478075
GENBANK/AF478076
GENBANK/AF478077
GENBANK/AF478078
GENBANK/AF478079
GENBANK/AF478080
GENBANK/AF478081
GENBANK/AF478082
GENBANK/AF478083
GENBANK/AF478084
GENBANK/AF478085
GENBANK/AF478086
GENBANK/AF478087
GENBANK/AF478088
GENBANK/AF478089
GENBANK/AF478090
GENBANK/AF478091
GENBANK/AF478092
GENBANK/AF478093
GENBANK/AF478094
GENBANK/AF478095
GENBANK/AF478096
GENBANK/AF478097
GENBANK/AF478098
GENBANK/AF478099
GENBANK/AF478100
GENBANK/AF478101
GENBANK/AF478102
GENBANK/AF478103
GENBANK/AF478104
GENBANK/AF478105
GENBANK/AF478106
GENBANK/AF478107
GENBANK/AF478108
GENBANK/AF478109
GENBANK/AF478110
GENBANK/AF478111
3' Untranslated Regions/*genetics
5' Untranslated Regions/*genetics
Bacillus/*classification/genetics
DNA, Ribosomal/analysis
DNA, Ribosomal Spacer/*analysis
Genes, rRNA
Molecular Sequence Data
*Phylogeny
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/genetics
*Sequence Analysis, DNA
Species Specificity
|
Remarks: |
The nucleotide sequences of the 3' end of the 16S rDNA and the 16S-23S internal transcribed spacer (ITS) of 40 Bacillaceae species were determined. These included 21 Bacillus, 9 Paenibacillus, 6 Brevibacillus, 2 Geobacillus, 1 Marinibacillus and 1 Virgibacillus species. Comparative sequence analysis of a 220 bp region covering a highly conserved 150 bp sequence located at the 3' end of the 16S rRNA coding region and a conserved 70 bp sequence located at the 5' end of the 16S-23S ITS of the 40 species and six sequences available in GenBank were used to infer the phylogenetic relationships between all 46 taxa. When a maximal distance (D(max), where D refers to the number of nucleotide substitutions per site) of 0.31 was introduced as a threshold to determine groupings, 10 phylogenetically distinct clusters were revealed. Twenty-six Bacillus species were separated in seven groups (I, II, III, IV, V, VI and X), but Bacillus circulans remained ungrouped. All six Brevibacillus species under study were in Group VII. The nine Paenibacillus species fell into two distinct groups (VIII and IX). Species with D(max) values within 0.05 were considered to be very closely related. These were Bacillus psychrophilus and Bacillus psychrosaccharolyticus in Group II; 'Bacillus maroccanus' and Bacillus simplex in Group II; Bacillus amyloliquefaciens, Bacillus atrophaeus, Bacillus mojavensis and Bacillus subtilis in Group VI; Bacillus fusiformis and Bacillus sphaericus in Group VI; Brevibacillus brevis and Brevibacillus formosus in Group VII; Paenibacillus gordonae and Paenibacillus validus in Group VIII; and Bacillus anthracis, Bacillus cereus, Bacillus mycoides and Bacillus thuringiensis in Group X. The phylogenetic classification presented here is, in general, in agreement with current classifications based on phenotypic and molecular data. Our findings suggest, however, that in some cases, further divisions or, conversely, further groupings might be warranted. Should current classifications be re-examined in the light of our results, D(max) values of 0.31 and 0.05, as exemplified here, may prove useful threshold values for the grouping of Bacillaceae into taxa akin to genera and species, respectively. These D(max) thresholds may also reveal, in a different way, bacterial species for which further characterization might be warranted for proper classification and/or reassignment. |
URL: |
12807189 |
|
Ref #: |
1300 |
Author(s): |
Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed) |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Approved Lists of Bacterial Names. |
Volume: |
30 |
Page(s): |
225-420 |
Year: |
1980 |
|
Ref #: |
2297 |
Author(s): |
Krych,V.K.;Johnson,J.L.;Yousten,A.A. |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Deoxyribonucleic acid homologies among strains of Bacillus sphaericus. |
Volume: |
30 |
Page(s): |
476-484 |
Year: |
1980 |
|
Ref #: |
2302 |
Author(s): |
Myers,P.;Yousten,A.A. |
Title: |
Cell wall structure of an entomocidal strain of Bacillus sphaericus. |
Page(s): |
31-35 |
Year: |
1978 |
|
Ref #: |
2306 |
Author(s): |
White,P.J.;Lotay,H.K. |
Journal: |
J. Gen. Microbiol. |
Title: |
Minimal nutritional requirements of Bacillus sphaericus NCTC 9602 and 26 other strains of this species: the majority grow and sporulate with acetate as sole major source of carbon. |
Volume: |
118 |
Page(s): |
13-19 |
Year: |
1980 |
|
Ref #: |
2459 |
Author(s): |
deBarjac,H.;Veran,M.;CosmaoDumanoir,V. |
Journal: |
Ann. Microbiol. (Inst. Pasteur) |
Title: |
Charactérisation biochimique et sérologique de souches de Bacillus sphaericus pathogènes ou non pour les moustiques. |
Volume: |
131 |
Page(s): |
191-201 |
Year: |
1980 |
|
Ref #: |
3768 |
Author(s): |
Fahmy,F.;Flossdorf,J.;Claus,D. |
Journal: |
System. Appl. Microbiol. |
Title: |
The DNA base composition of the type strains of the genus Bacillus. |
Volume: |
6 |
Page(s): |
60-65 |
Year: |
1985 |
|
|
Data: |
(Kral Marburg strain Wund Neide, ATCC 14577, IFO 15095, NCIB 9370) Type strain / T. Gibson, Edinburgh in 1963 / Neide, E. (1904) Zentbl. Bakt. ParasitKde, II. Abt. 12, 350 / Smith, N. R. et al. (1964) J. gen. Microbiol. 34, 269
|
Accession Date: |
01/01/1963
|
History: |
EDINBURGH SCHOOL OF AGRICULTURE
|
Authority: |
Meyer and Neide 1904 (AL)
|
Depositor: |
GIBSON T
|
Taxonomy: |
TaxLink: S434 (Lysinibacillus sphaericus (meyer and neide 1904) ahmed et al. 2007) - Date of change: 16/06/2007 by NCTCUp to 16/06/2007: ? (NCTC 10338) - Date of change: 04/02/2003
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Biosafety Responsibility: |
It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country
|
The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.
Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.