Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Bacillus pumilus

NCTC Number: NCTC 10337
Current Name: Bacillus pumilus
Original Strain Reference: Kral
Other Collection No: ATCC 7061; CCM 2144; DSM 27; GOTTHEIL; IFO 12092; JCM 2508; KRAL; LOHNIS; N R SMITH 272; NCIB 9369
Previous Catalogue Name: Bacillus pumilus
Type Strain: Yes
Family: Bacillaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, aerobic
Whole Genome Sequence:
16S rRNA Gene Sequence: >gb|AF478070|ATCC 7061|Bacillus pumilus clone Bpumi-1F 16S ribosomal RNA gene, partialsequence; 16S-23S internal transcribed spacer, complete sequence;and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata... >gb|AY876289|ATCC 7061|Bacillus pumilus strain ATCC 7061 16S ribosomal RNA gene, partialsequence.| tgcagtcgagcggac...
23S rRNA Gene Sequence: >gb|AF478070|ATCC 7061|Bacillus pumilus clone Bpumi-1F 16S ribosomal RNA gene, partialsequence; 16S-23S internal transcribed spacer, complete sequence;and 23S ribosomal RNA gene, partial sequence.| atgccgcggtgaata...
Extended Bibliography: showhide Show bibliography
Ref #: 22093
Author(s): Xu,D.;Cote,J.C.
Journal: Int J Syst Evol Microbiol
Title: Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3' end 16S rDNA and 5' end 16S-23S ITS nucleotide sequences
Volume: 53
Page(s): 695-704
Year: 2003
Keyword(s): GENBANK/AF478062 GENBANK/AF478063 GENBANK/AF478064 GENBANK/AF478065 GENBANK/AF478066 GENBANK/AF478067 GENBANK/AF478068 GENBANK/AF478069 GENBANK/AF478070 GENBANK/AF478071 GENBANK/AF478072 GENBANK/AF478073 GENBANK/AF478074 GENBANK/AF478075 GENBANK/AF478076 GENBANK/AF478077 GENBANK/AF478078 GENBANK/AF478079 GENBANK/AF478080 GENBANK/AF478081 GENBANK/AF478082 GENBANK/AF478083 GENBANK/AF478084 GENBANK/AF478085 GENBANK/AF478086 GENBANK/AF478087 GENBANK/AF478088 GENBANK/AF478089 GENBANK/AF478090 GENBANK/AF478091 GENBANK/AF478092 GENBANK/AF478093 GENBANK/AF478094 GENBANK/AF478095 GENBANK/AF478096 GENBANK/AF478097 GENBANK/AF478098 GENBANK/AF478099 GENBANK/AF478100 GENBANK/AF478101 GENBANK/AF478102 GENBANK/AF478103 GENBANK/AF478104 GENBANK/AF478105 GENBANK/AF478106 GENBANK/AF478107 GENBANK/AF478108 GENBANK/AF478109 GENBANK/AF478110 GENBANK/AF478111 3' Untranslated Regions/*genetics 5' Untranslated Regions/*genetics Bacillus/*classification/genetics DNA, Ribosomal/analysis DNA, Ribosomal Spacer/*analysis Genes, rRNA Molecular Sequence Data *Phylogeny RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/genetics *Sequence Analysis, DNA Species Specificity
Remarks: The nucleotide sequences of the 3' end of the 16S rDNA and the 16S-23S internal transcribed spacer (ITS) of 40 Bacillaceae species were determined. These included 21 Bacillus, 9 Paenibacillus, 6 Brevibacillus, 2 Geobacillus, 1 Marinibacillus and 1 Virgibacillus species. Comparative sequence analysis of a 220 bp region covering a highly conserved 150 bp sequence located at the 3' end of the 16S rRNA coding region and a conserved 70 bp sequence located at the 5' end of the 16S-23S ITS of the 40 species and six sequences available in GenBank were used to infer the phylogenetic relationships between all 46 taxa. When a maximal distance (D(max), where D refers to the number of nucleotide substitutions per site) of 0.31 was introduced as a threshold to determine groupings, 10 phylogenetically distinct clusters were revealed. Twenty-six Bacillus species were separated in seven groups (I, II, III, IV, V, VI and X), but Bacillus circulans remained ungrouped. All six Brevibacillus species under study were in Group VII. The nine Paenibacillus species fell into two distinct groups (VIII and IX). Species with D(max) values within 0.05 were considered to be very closely related. These were Bacillus psychrophilus and Bacillus psychrosaccharolyticus in Group II; 'Bacillus maroccanus' and Bacillus simplex in Group II; Bacillus amyloliquefaciens, Bacillus atrophaeus, Bacillus mojavensis and Bacillus subtilis in Group VI; Bacillus fusiformis and Bacillus sphaericus in Group VI; Brevibacillus brevis and Brevibacillus formosus in Group VII; Paenibacillus gordonae and Paenibacillus validus in Group VIII; and Bacillus anthracis, Bacillus cereus, Bacillus mycoides and Bacillus thuringiensis in Group X. The phylogenetic classification presented here is, in general, in agreement with current classifications based on phenotypic and molecular data. Our findings suggest, however, that in some cases, further divisions or, conversely, further groupings might be warranted. Should current classifications be re-examined in the light of our results, D(max) values of 0.31 and 0.05, as exemplified here, may prove useful threshold values for the grouping of Bacillaceae into taxa akin to genera and species, respectively. These D(max) thresholds may also reveal, in a different way, bacterial species for which further characterization might be warranted for proper classification and/or reassignment.
URL: 12807189
Ref #: 24297
Author(s): Satomi,M.;La Duc,M.T.;Venkateswaran,K.
Journal: Int J Syst Evol Microbiol
Title: Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces
Volume: 56
Page(s): 1735-40
Year: 2006
Keyword(s): Bacillus/*classification/genetics/isolation & purification Bacterial Proteins/genetics DNA Fingerprinting DNA Gyrase/genetics DNA, Bacterial/genetics *Environmental Microbiology Nucleic Acid Hybridization Phylogeny Polymerase Chain Reaction RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Repetitive Sequences, Nucleic Acid/genetics Sequence Homology, Nucleic Acid *Spacecraft Species Specificity
Remarks: Thirteen strains of a novel spore-forming, Gram-positive, mesophilic heterotrophic bacterium were isolated from spacecraft surfaces (Mars Odyssey Orbiter) and assembly-facility surfaces at the Jet Propulsion Laboratory in California and the Kennedy Space Center in Florida. Phylogenetic analysis of 16S rRNA gene sequences has placed these novel isolates within the genus Bacillus, the greatest sequence similarity (99.9 %) being found with Bacillus pumilus. However, these isolates share a mere 91.2 % gyrB sequence similarity with Bacillus pumilus, rendering their 16S rRNA gene-derived relatedness suspect. Furthermore, DNA-DNA hybridization showed only 54-66 % DNA relatedness between the novel isolates and strains of B. pumilus. rep-PCR fingerprinting and previously reported matrix-assisted laser desorption/ionization time-of-flight mass spectrometry protein profiling clearly distinguished these isolates from B. pumilus. Phenotypic analyses also showed some differentiation between the two genotypic groups, although the fatty acid compositions were almost identical. The polyphasic taxonomic studies revealed distinct clustering of the tested strains into two distinct species. On the basis of phenotypic characteristics and the results of phylogenetic analyses of 16S rRNA and gyrB gene sequences, repetitive element primer-PCR fingerprinting and DNA-DNA hybridization, the 13 isolates represent a novel species of the genus Bacillus, for which the name Bacillus safensis sp. nov. is proposed. The type strain is FO-36b(T) (=ATCC BAA-1126(T)=NBRC 100820(T)).
URL: 16902000
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 3768
Author(s): Fahmy,F.;Flossdorf,J.;Claus,D.
Journal: System. Appl. Microbiol.
Title: The DNA base composition of the type strains of the genus Bacillus.
Volume: 6
Page(s): 60-65
Year: 1985
Ref #: 4714
Author(s): Seki,T.;Chung,C.-K.;Mikami,H.;Oshima,Y.
Journal: Int. J. Syst. Bacteriol.
Title: Deoxyribonucleic acid homology and taxonomy of the genus Bacillus.
Volume: 28
Page(s): 182-189
Year: 1978
Data: (N. R. Smith 272, Lohnis, Gottheil, ATCC 7061, NCIB 9369) Type strain / T. Gibson, Edinburgh in 1963 / Gottheil, O. (1901) Zentbl. Bakt. ParasitKde, II. Abt. 7, 681 / Smith, N. R. et al. (1964) J. gen. Microbiol. 34, 269
Accession Date: 01/01/1963
Authority: Meyer and Gottheil 1901 (AL)
Depositor: GIBSON T
Taxonomy: TaxLink: S430 (Bacillus pumilus Meyer and Gottheil 1901) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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