Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Yersinia pseudotuberculosis

NCTC Number: NCTC 10275
Current Name: Yersinia pseudotuberculosis
Original Strain Reference: 14/1
Other Collection No: ATCC 29833; CIP 55.85; DSM 8992
Previous Catalogue Name: Yersinia pseudotuberculosis
Type Strain: Yes
Family: Enterobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: bird;from a turkey
Whole Genome Sequence:
16S rRNA Gene Sequence: >gb|AF366375|ATCC 29833|Yersinia pseudotuberculosis 16S ribosomal RNA gene, partialsequence.| attgaacgctggcgg...
Miscellaneous Sequence Data: >gb|AY370874|ATCC 29833|Yersinia pseudotuberculosis strain ATCC 29833 gyrase B (gyrB) gene,partial cds.| aaagtttccggcggt...
Extended Bibliography: showhide Show bibliography
Ref #: 95482
Author(s): Kim,W.;Song,M.O.;Song,W.;Kim,K.J.;Chung,S.I.;Choi,C.S.;Park,Y.H.
Journal: Antonie Van Leeuwenhoek
Title: Comparison of 16S rDNA analysis and rep-PCR genomic fingerprinting for molecular identification of Yersinia pseudotuberculosis
Volume: 83
Page(s): 125-33
Year: 2003
Keyword(s): GENBANK/AF365934 GENBANK/AF365935 GENBANK/AF365936 GENBANK/AF365937 GENBANK/AF365938 GENBANK/AF365939 GENBANK/AF365940 GENBANK/AF365941 GENBANK/AF365942 GENBANK/AF365943 GENBANK/AF365944 GENBANK/AF365945 GENBANK/AF365946 GENBANK/AF365947 GENBANK/AF365948 GENBANK/AF365949 GENBANK/AF365950 GENBANK/AF366375 GENBANK/AF366376 GENBANK/AF366377 GENBANK/AF366378 GENBANK/AF366379 GENBANK/AF366380 GENBANK/AF366381 GENBANK/AF366382 GENBANK/AF366383 GENBANK/AF366384 GENBANK/AF366385 DNA Fingerprinting/*methods DNA, Bacterial/analysis DNA, Ribosomal/analysis Genome, Bacterial Humans Molecular Sequence Data Phylogeny Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/*genetics *Sequence Analysis, DNA Yersinia pestis/classification/genetics Yersinia pseudotuberculosis/*classification/genetics
Remarks: 16S rDNA sequence analysis and repetitive element sequence-based PCR (rep-PCR) genomic fingerprinting were evaluated on 11 type strains of the genus Yersinia and 17 recognized serotype strains of Y. pseudotuberculosis to investigate their genetic relatedness and to establish the value of techniques for the identification of Y. pseudotuberculosis. A phylogenetic tree constructed from 16S rDNA sequences showed that the type strains of Yersinia species formed distinct clusters with the exception of Y. pestis and Y. pseudotuberculosis. Moreover, Y. pestis NCTC 5923T was found to be closely related to Y. pseudotuberculosis serotypes 1b, 3, and 7. Dendrograms generated from REP-PCR, and ERIC-PCR data revealed that members of the genus Yersinia differed from each other with the degree of similarity 62% and 58%, respectively. However, the BOX-PCR results showed that Y. pestis 5923T clustered with the Y. pseudotuberculosis group with a degree of similarity 74%. According to these findings, 16S rDNA sequence analysis was unable to reliably discriminate Y. pseudotuberculosis from Y. pestis. However, REP-PCR and especially ERIC-PCR provided an effective means of differentiating between members of the taxa.
URL: 12785306
Ref #: 43181
Author(s): Delmas,J.;Breysse,F.;Devulder,G.;Flandrois,J.P.;Chomarat,M.
Journal: Diagn Microbiol Infect Dis
Title: Rapid identification of Enterobacteriaceae by sequencing DNA gyrase subunit B encoding gene
Volume: 55
Page(s): 263-8
Year: 2006
Keyword(s): Bacterial Typing Techniques/*methods DNA Gyrase/*genetics Enterobacteriaceae/enzymology/genetics/*isolation & purification Genotype Humans Sequence Analysis, DNA/methods
Remarks: Real-time polymerase chain reaction and sequencing were used to characterize a 506-bp-long DNA fragment internal to the gyrB gene (gyrBint). The sequences obtained from 32 Enterobacteriaceae-type strains and those available in the Genbank nucleotide sequence database (n = 24) were used as a database to identify 240 clinical enterobacteria isolates. Sequence analysis of the gyrBint fragment of 240 strains showed that gyrBint constitutes a discriminative target sequence to differentiate between Enterobacteriaceae species. Comparison of these identifications with those obtained by phenotypic methods (Vitek 1 system and/or Rapid ID 32E; bioMerieux, Marcy l'Etoile, France) revealed discrepancies essentially with genera Citrobacter and Enterobacter. Most of the strains identified as Enterobacter cloacae by phenotypic methods were identified as Enterobacter hormaechei strains by gyrBint sequencing. The direct sequencing of gyrBint would be useful as a complementary tool in the identification of clinical Enterobacteriaceae isolates.
URL: 16626902
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 5069
Author(s): Bercovier,H.;Mollaret,H.H.
Title: Genus XIV. Yersinia.
Page(s): 498-506
Ref #: 7653
Author(s): Smith,J.E.;Thal,E.
Journal: Acta Pathol. Microbiol. Scand.
Title: A taxonomic study of the genus Pasteurella using a numerical technique.
Volume: 64
Page(s): 213-223
Year: 1965
Data: (ATCC 29833) Type strain / E. Thal, Stockholm in 1962 / Turkey in 1952 / Thal group 1
Accession Date: 01/01/1962
History: IDOLATED IN 1952
Authority: (Pfeiffer 1889) Smith and Thal 1965 (AL)
Depositor: THAL E
Taxonomy: TaxLink: S3618 (Yersinia pseudotuberculosis (Pfeiffer 1889) Smith and Thal 1965) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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