Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Mycobacterium peregrinum

NCTC Number: NCTC 10264
Current Name: Mycobacterium peregrinum
Original Strain Reference: 6020
Other Collection No: ATCC 14467; ATCC 23023; ATCC 23036; CCUG 27976; DSM 43271; IMET 10608; TMC 1547
Previous Catalogue Name: Mycobacterium peregrinum
Type Strain: Yes
Family: Mycobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48-96 hours, 37°C, aerobic
Conditions for growth on liquid media: glucose broth,37, aerobic
Isolated From: human, bronchial aspirate of child
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1497473
16S rRNA Gene Sequence: >gb|AF130308|ATCC14467(T)|Mycobacterium peregrinum 16S ribosomal RNA gene, partial sequence.| gacgaacgctggcgg... >gb|AJ422046|ATCC 14467|Mycobacterium peregrinum partial 16S rRNA gene, strain ATCC14467.| gcggtgtgggatggg... >gb|AF058712|ATCC14467| NCTC10264|Mycobacterium peregrinum 16S ribosomal RNA gene, partial sequence.| acacatgcaagtcga...
Miscellaneous Sequence Data: >gb|AJ564413|ATCC 14467|Mycobacterium peregrinum partial GyrB gene for DNA gyrase subunitB, strain ATCC 14467.| gattcggccggcggc...
Bibliography: BOJALIL L F ET AL 1962 J GEN MICROBIOL 28 333
Extended Bibliography: showhide Show bibliography
Ref #: 60196
Author(s): Kim,B.J.;Lee,S.H.;Lyu,M.A.;Kim,S.J.;Bai,G.H.;Chae,G.T.;Kim,E.C.;Cha,C.Y.;Kook,Y.H.
Journal: J Clin Microbiol
Title: Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB)
Volume: 37
Page(s): 1714-20
Year: 1999
Keyword(s): GENBANK/AF057449 GENBANK/AF057450 GENBANK/AF057451 GENBANK/AF057452 GENBANK/AF057453 GENBANK/AF057454 GENBANK/AF057455 GENBANK/AF057456 GENBANK/AF057457 GENBANK/AF057458 GENBANK/AF057459 GENBANK/AF057460 GENBANK/AF057461 GENBANK/AF057462 GENBANK/AF057463 GENBANK/AF057464 GENBANK/AF057465 GENBANK/AF057466 GENBANK/AF057467 GENBANK/AF057468 GENBANK/AF057469 GENBANK/AF057470 GENBANK/AF057471 GENBANK/AF057472 GENBANK/AF057473 GENBANK/AF057474 GENBANK/AF057475 GENBANK/AF057476 GENBANK/AF057477 GENBANK/AF057478 etc. Amino Acid Sequence DNA-Directed RNA Polymerases/chemistry/*genetics Humans Molecular Sequence Data Mycobacterium/*classification/enzymology/genetics Mycobacterium Infections/microbiology Phylogeny Restriction Mapping Sequence Alignment Sequence Homology, Amino Acid
Remarks: For the differentiation and identification of mycobacterial species, the rpoB gene, encoding the beta subunit of RNA polymerase, was investigated. rpoB DNAs (342 bp) were amplified from 44 reference strains of mycobacteria and clinical isolates (107 strains) by PCR. The nucleotide sequences were directly determined (306 bp) and aligned by using the multiple alignment algorithm in the MegAlign package (DNASTAR) and the MEGA program. A phylogenetic tree was constructed by the neighbor-joining method. Comparative sequence analysis of rpoB DNAs provided the basis for species differentiation within the genus Mycobacterium. Slowly and rapidly growing groups of mycobacteria were clearly separated, and each mycobacterial species was differentiated as a distinct entity in the phylogenetic tree. Pathogenic Mycobacterium kansasii was easily differentiated from nonpathogenic M. gastri; this differentiation cannot be achieved by using 16S rRNA gene (rDNA) sequences. By being grouped into species-specific clusters with low-level sequence divergence among strains of the same species, all of the clinical isolates could be easily identified. These results suggest that comparative sequence analysis of amplified rpoB DNAs can be used efficiently to identify clinical isolates of mycobacteria in parallel with traditional culture methods and as a supplement to 16S rDNA gene analysis. Furthermore, in the case of M. tuberculosis, rifampin resistance can be simultaneously determined.
URL: 10325313
Ref #: 60037
Author(s): Hamid,M.E.;Roth,A.;Landt,O.;Kroppenstedt,R.M.;Goodfellow,M.;Mauch,H.
Journal: J Clin Microbiol
Title: Differentiation between Mycobacterium farcinogenes and Mycobacterium senegalense strains based on 16S-23S ribosomal DNA internal transcribed spacer sequences
Volume: 40
Page(s): 707-11
Year: 2002
Keyword(s): GENBANK/AJ291580 GENBANK/AJ291581 GENBANK/AJ291582 GENBANK/AJ291583 GENBANK/AJ291584 GENBANK/AJ291585 GENBANK/AJ291586 GENBANK/AJ291587 GENBANK/AJ291588 GENBANK/AJ291589 GENBANK/AJ291590 GENBANK/AJ291591 GENBANK/AJ291592 GENBANK/AJ291593 GENBANK/AJ291594 GENBANK/AJ291595 GENBANK/AJ291596 GENBANK/AJ291597 GENBANK/AJ291598 GENBANK/AJ291599 GENBANK/AJ291600 GENBANK/Y10384 GENBANK/Y10385 GENBANK/Y11581 GENBANK/Y11582 Animals Base Sequence Cattle Cattle Diseases/*microbiology DNA, Ribosomal Spacer/*genetics Molecular Sequence Data Mycobacterium/*classification/genetics Mycobacterium Infections/microbiology/*veterinary Phylogeny Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA
Remarks: 16S ribosomal DNA (rDNA) and 16S-23S internal transcribed spacer rDNA sequence analyses were performed on Mycobacterium farcinogenes and M. senegalense strains and 26 strains of other rapidly growing mycobacteria to investigate the phylogenetic structure of bovine farcy mycobacteria within the M. fortuitum complex. M. farcinogenes and M. senegalense were indistinguishable in their 5"-end 16S rDNA but showed both considerable interspecies spacer sequence divergence and a high level of intraspecies sequence stability. A rapid detection assay using PCR and hybridization with species-specific probes was developed. The assay was specific among 46 species other than M. farcinogenes and M. senegalense and correctly identified all M. farcinogenes and M. senegalense strains. PCR- and 16S-23S rDNA sequence-based detection will be a valuable approach for diagnosis of the causal agents of African bovine farcy in cattle.
URL: 11826003
Ref #: 61372
Author(s): Schinsky,M.F.;McNeil,M.M.;Whitney,A.M.;Steigerwalt,A.G.;Lasker,B.A.;Floyd,M.M.;Hogg,G.G.;Brenner,D.J.;Brown,J.M.
Journal: Int J Syst Evol Microbiol
Title: Mycobacterium septicum sp. nov., a new rapidly growing species associated with catheter-related bacteraemia
Volume: 50 Pt 2
Page(s): 575-81
Year: 2000
Keyword(s): GENBANK/AF111809 Bacteremia/*microbiology Base Composition *Catheterization, Central Venous Catheters, Indwelling/*microbiology Chromatography, High Pressure Liquid DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics Genes, rRNA Genotype Humans Molecular Sequence Data Mycobacterium/*classification/*isolation & purification/physiology Mycobacterium Infections/*microbiology Mycolic Acids/analysis Nucleic Acid Hybridization Phenotype Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA
Remarks: Rapidly growing mycobacteria are capable of causing several clinical diseases in both immunosuppressed and immunocompetent individuals. A previously unidentified, rapidly growing mycobacterium was determined to be the causative agent of central line sepsis in a child with underlying metastatic hepatoblastoma. Four isolates of this mycobacterium, three from blood and one from the central venous catheter tip, were studied. Phenotypic characterization, HPLC and genetic analysis revealed that while this organism most closely resembled members of the Mycobacterium fortuitum complex and Mycobacterium senegalense, it differed from all previously described species. Phenotypic tests useful in differentiating this species from similar rapidly growing mycobacteria included: growth at 42 degrees C, hydrolysis of acetamide, utilization of citrate, production of arylsulfatase (3-d), acidification of D-mannitol and i-myo-inositol, and susceptibility to erythromycin, vancomycin and tobramycin. The name Mycobacterium septicum is proposed for this new species. The type strain has been deposited in Deutsche Sammlung von Mikroorganismen und Zellkulturen as DSM 44393T and in the American Type Culture Collection as strain ATCC 700731T.
URL: 10758863
Ref #: 95465
Author(s): Dauendorffer,J.N.;Guillemin,I.;Aubry,A.;Truffot-Pernot,C.;Sougakoff,W.;Jarlier,V.;Cambau,E.
Journal: J Clin Microbiol
Title: Identification of mycobacterial species by PCR sequencing of quinolone resistance-determining regions of DNA gyrase genes
Volume: 41
Page(s): 1311-5
Year: 2003
Keyword(s): Anti-Infective Agents/*pharmacology Base Sequence DNA Gyrase/*genetics DNA, Bacterial/analysis Drug Resistance, Bacterial/*genetics Fluoroquinolones Humans Microbial Sensitivity Tests Molecular Sequence Data Mycobacterium/drug effects/enzymology/*genetics Polymerase Chain Reaction Protein Structure, Tertiary/genetics Sequence Homology, Nucleic Acid
Remarks: The determination of the amino acid sequence of quinolone resistance-determining regions (QRDRs) in the A and B subunits of DNA gyrase is the molecular test for the detection of fluoroquinolone resistance in mycobacteria. We looked to see if the assignment of mycobacterial species could be obtained simultaneously by analysis of the corresponding nucleotide sequences. PCR sequencing of gyrA and gyrB QRDRs was performed for 133 reference and clinical strains of 21 mycobacterial species commonly isolated in clinical laboratories. Nucleotide sequences of gyrA and gyrB QRDRs were species specific, regardless of fluoroquinolone susceptibility.
URL: 12624075
Ref #: 13682
Author(s): Hamid,M.E.;Roth,A.;Landt,O.;Kroppenstedt,R.M.;Goodfellow,M.;Mauch,H.
Journal: J Clin Microbiol
Title: Differentiation between Mycobacterium farcinogenes and Mycobacterium
Volume: 40
Page(s): 707-711
Year: 2002
Keyword(s): 0 (DNA, Ribosomal Spacer) 0 (RNA, Ribosomal, 16S) 0 (RNA, Ribosomal, 23S) Animal Base Sequence Cattle Cattle Diseases/*microbiology DNA, Ribosomal Spacer/*genetics Molecular Sequence Data Mycobacterium/*classification/genetics Mycobacterium Infections/microbiology/*veterinary Phylogeny Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Support, Non-U.S. Gov't
Remarks: 16S ribosomal DNA (rDNA) and 16S-23S internal transcribed spacer rDNA
URL: 21683667
Ref #: 13200
Author(s): Schinsky,M.F.;McNeil,M.M.;Whitney,A.M.;Steigerwalt,A.G.;Lasker,B.A.;Floyd,M.M.;Hogg,G.G.;Brenner,D.J.;Brown,J.M.
Journal: Int J Syst Evol Microbiol
Title: Mycobacterium septicum sp. nov., a new rapidly growing species associated with catheter-related bacteraemia
Volume: 50 Pt 2
Page(s): 575-81
Year: 2000
Keyword(s): GENBANK/AF111809 Bacteremia/*microbiology Base Composition *Catheterization, Central Venous Catheters, Indwelling/*microbiology Chromatography, High Pressure Liquid DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics Genes, rRNA Genotype Human Molecular Sequence Data Mycobacterium/*classification/*isolation & purification/physiology Mycobacterium Infections/*microbiology Mycolic Acids/analysis Nucleic Acid Hybridization Phenotype Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA
Remarks: Rapidly growing mycobacteria are capable of causing several clinical diseases in both immunosuppressed and immunocompetent individuals. A previously unidentified, rapidly growing mycobacterium was determined to be the causative agent of central line sepsis in a child with underlying metastatic hepatoblastoma. Four isolates of this mycobacterium, three from blood and one from the central venous catheter tip, were studied. Phenotypic characterization, HPLC and genetic analysis revealed that while this organism most closely resembled members of the Mycobacterium fortuitum complex and Mycobacterium senegalense, it differed from all previously described species. Phenotypic tests useful in differentiating this species from similar rapidly growing mycobacteria included: growth at 42 degrees C, hydrolysis of acetamide, utilization of citrate, production of arylsulfatase (3-d), acidification of D-mannitol and i-myo-inositol, and susceptibility to erythromycin, vancomycin and tobramycin. The name Mycobacterium septicum is proposed for this new species. The type strain has been deposited in Deutsche Sammlung von Mikroorganismen und Zellkulturen as DSM 44393T and in the American Type Culture Collection as strain ATCC 700731T.
URL: 20222162
Ref #: 4709
Author(s): Kusunoki,S.;Ezaki,T.
Journal: Int. J. Syst. Bacteriol.
Title: Proposal of Mycobacterium peregrinum sp. nov., nom rev., and elevation of Mycobacterium chelonae subsp. abscessus (Kubica et al.) to species status: Mycobacterium abscessus comb. nov.
Volume: 42
Page(s): 240-245
Year: 1992
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Data: (ATCC 14467, TMC 1547) Type strain / L. F. Bojalil, Mexico in 1961 / Bronchial aspirate of child / Bojalil, L. F. et al. (1962) J. gen. Microbiol. 28, 333
Accession Date: 01/01/1961
History: BOJALIL L F,UNIDAD DE PATOLOGIA,ESCUELA DE MEDICINA,HOSP GEN PRE:FR
Authority: Kusunoki and Ezaki 1992
Depositor: BOJALIL L F
Taxonomy: TaxLink: S4295 (Mycobacterium peregrinum Kusunoki and Ezaki 1992) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

Note: Links open in a new window

Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

Contact us if you want to discuss commercial use of the cultures.

Available Formats

Ampoule (Bacteria)

Back to top
Copyright © Public Health England.

Please confirm your country of origin from the list below.