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Bacteria Collection: Pasteurella multocida subsp. gallicida

NCTC Number: NCTC 10204
Current Name: Pasteurella multocida subsp. gallicida
Original Strain Reference: 739
Other Collection No: ATCC 51689; CCUG 17978; CIP 103285
Previous Catalogue Name: Pasteurella multocida
Type Strain: Yes
Family: Pasteurellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar roberts type iv
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: blood agar,nutrient broth, (peptone water sugars),37, facultative
Isolated From: mammal, bovine
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1003584
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AY078998|NCTC 10204|Pasteurella multocida subsp. gallicida strain NCTC 10204 16Sribosomal RNA gene, partial sequence.| tcagattgaacgctg...
Bibliography: ROBERTS R S 1947 J COMP PATH THER 57 261;BYTHELL D W P 1945 VET REC 57 289
Extended Bibliography: showhide Show bibliography
Ref #: 67383
Author(s): Davies,R.L.
Journal: Microbiology
Title: Genetic diversity among Pasteurella multocida strains of avian, bovine, ovine and porcine origin from England and Wales by comparative sequence analysis of the 16S rRNA gene
Volume: 150
Page(s): 4199-210
Year: 2004
Keyword(s): GENBANK/AY078996 GENBANK/AY078998 GENBANK/AY078999 GENBANK/AY079000 GENBANK/AY299304 GENBANK/AY299305 GENBANK/AY299306 GENBANK/AY299307 GENBANK/AY299308 GENBANK/AY299309 GENBANK/AY299310 GENBANK/AY299311 GENBANK/AY299312 GENBANK/AY299313 GENBANK/AY299314 GENBANK/AY299315 GENBANK/AY299316 GENBANK/AY299317 GENBANK/AY299318 GENBANK/AY299319 GENBANK/AY324032 Animals Base Sequence Cats Cattle/microbiology England Galactitol/metabolism Genes, rRNA Molecular Sequence Data Pasteurella Infections/microbiology/+ACo-veterinary Pasteurella multocida/+ACo-classification/genetics/metabolism Phylogeny Poultry/microbiology RNA, Ribosomal, 16S/+ACo-genetics Sequence Analysis, DNA Sheep/microbiology Sorbitol/metabolism Swine/microbiology +ACo-Variation (Genetics) Wales
Remarks: Genetic diversity among 86 Pasteurella multocida isolates was investigated by comparative sequence analysis of a 1468 bp fragment of the 16S rRNA gene. The strains included 79 field isolates recovered from birds (poultry) (22), cattle (21), pigs (26) and sheep (10) within England and Wales, four Asian isolates associated with bovine haemorrhagic septicaemia, and the type strains of the three subspecies of P. multocida. Dulcitol and sorbitol fermentation patterns were also determined to establish correlations between subspecies status and phylogenetic relatedness. Nineteen 16S rRNA types were identified, but these were clustered into two distinct phylogenetic lineages, A and B. Sequences within lineages A and B had a mean number of nucleotide differences of 21.123.90. Isolates within lineage A were associated with birds, cattle, pigs and sheep, whereas those belonging to lineage B were recovered from birds and a cat. Eighty-seven per cent of the isolates were classified as P. multocida subsp. multocida by dulcitol and sorbitol fermentation patterns, but these have diverse 16S rRNA gene sequences that were represented in both lineages A and B. Avian P. multocida subsp. septica isolates were associated exclusively with lineage B, but bovine P. multocida subsp. septica isolates were present in lineage A. P. multocida subsp. gallicida isolates of avian, bovine and porcine origin represent a homogeneous group within lineage A, but they have the same 16S rRNA type as certain P. multocida subsp. multocida isolates. These findings provide strong support for the view that dulcitol and sorbitol fermentation patterns are inaccurate indicators of genetic relatedness among P. multocida strains. Avian capsular type B isolates and capsular type B and E isolates associated with haemorrhagic septicaemia of cattle and water buffaloes are closely related and form a distinct cluster within lineage A. The current subspecies nomenclature of P. multocida neither accurately reflects the 16S rRNA-based phylogenetic relationships among isolates nor does it adequately encompass the full range of diversity within the species. The study provides a 16S rRNA-based evolutionary framework that will form the basis of further studies into the genetic diversity of P. multocida and will also help in the reclassification of the species.
URL: 15583172
Data: Type strain of ss galliseptica / R. S. Roberts, Runcorn, Cheshire in 1960 / Bovine, UK / Bythell, D. W. P. (1945) Vet. Rec. 57, 289 / Roberts, R. S. (1947) J. comp. Path. Ther. 57, 261 / Subsp. gallicida
Accession Date: 01/01/1960
History: ROBERTS R S, EVANS BIOL INST, RUNCORN, CHESHIRE
Authority: (Lehmann and Neumann 1899) Rosenbusch and Merchant 1939 (AL)
Depositor: ROBERTS R S
Taxonomy: TaxLink: S2224 (Pasteurella multocida subsp. multocida (Lehmann and Neumann 1899) Rosenbusch and Merchant 1939) - Date of change: 5/02/2003
Other: Roberts type IV
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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