Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Pseudomonas fluorescens

NCTC Number: NCTC 10038
Current Name: Pseudomonas fluorescens
Original Strain Reference: 28/5
Other Collection No: ATCC 13525; CCB 546; DSM 50090; ICPB 3200; NCIB 9046; RH 818; WDCM 00115; 28/5
Previous Catalogue Name: Pseudomonas fluorescens
Type Strain: Yes
Family: Pseudomonadaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 30°C, aerobic
Conditions for growth on liquid media: nutrient broth,30, aerobic
Isolated From: freshwater;pre-filter tanks ,reading water works
Whole Genome Sequence:
Annotated Genome:
16S rRNA Gene Sequence: >gb|AJ308308|ICMP 3512| ATCC 13525| NCIB 9046|Pseudomonas fluorescens partial 16S rRNA gene, isolate ICMP 3512;ATCC 13525; NCIB 9046.| agtgggggataacgt... >gb|AF094725|ATCC 13525|Pseudomonas fluorescens strain ATCC 13525 16S ribosomal RNA gene,partial sequence.| tggctcagattgaac... >gb|Z76662|DSM 50090T (TYPE STRAIN)|P.fluorescens 16S rRNA gene.| attgaacgctggcgg... >gb|DQ207731|CCM 2115| ATCC13525|Pseudomonas fluorescens strain CCM 2115 16S ribosomal RNA gene,complete sequence.| agagtttgatcctgg...
Extended Bibliography: showhide Show bibliography
Ref #: 95513
Author(s): Sipos,R.;Szekely,A.J.;Palatinszky,M.;Revesz,S.;Marialigeti,K.;Nikolausz,M.
Journal: FEMS Microbiol Ecol
Title: Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis
Volume: 60
Page(s): 341-50
Year: 2007
Remarks: In the attempt to explore complex bacterial communities of environmental samples, primers hybridizing to phylogenetically highly conserved regions of 16S rRNA genes are widely used, but differential amplification is a recognized problem. The biases associated with preferential amplification of multitemplate PCR were investigated using 'universal' bacteria-specific primers, focusing on the effect of primer mismatch, annealing temperature and PCR cycle number. The distortion of the template-to-product ratio was measured using predefined template mixtures and environmental samples by terminal restriction fragment length polymorphism analysis. When a 1 : 1 genomic DNA template mixture of two strains was used, primer mismatches inherent in the 63F primer presented a serious bias, showing preferential amplification of the template containing the perfectly matching sequence. The extent of the preferential amplification showed an almost exponential relation with increasing annealing temperature from 47 to 61 degrees C. No negative effect of the various annealing temperatures was observed with the 27F primer, with no mismatches with the target sequences. The number of PCR cycles had little influence on the template-to-product ratios. As a result of additional tests on environmental samples, the use of a low annealing temperature is recommended in order to significantly reduce preferential amplification while maintaining the specificity of PCR.
URL: 17343679
Ref #: 95451
Author(s): Hilario,E.;Buckley,T.R.;Young,J.M.
Journal: Antonie Van Leeuwenhoek
Title: Improved resolution on the phylogenetic relationships among Pseudomonas by the combined analysis of atp D, car A, rec A and 16S rDNA
Volume: 86
Page(s): 51-64
Year: 2004
Keyword(s): Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/*genetics DNA, Bacterial/chemistry/isolation & purification Evolution, Molecular Phylogeny Proton-Translocating ATPases/*genetics Pseudomonas/*classification/*genetics RNA, Ribosomal, 16S/*genetics Rec A Recombinases/*genetics Sequence Analysis, DNA
Remarks: A study of representatives of the bacterial genus Pseudomonas, analysing a combined data set of four molecular sequences with completely different properties and evolutionary constraints, is reported. The best evolutionary model was obtained with a hierarchical hypothesis testing program to describe each data set and the combined data set is presented and analysed under the likelihood criterion. The resolution among Pseudomonas taxa based on the combined data set analysis of the different lineages increased due to a synergistic effect of the individual data sets. The unresolved fluorescens lineage, as well as other weakly supported lineages in the single data set trees, should be revised in detail at the biochemical and molecular level. The taxonomic status of biovars of P. putida is discussed.
URL: 15103237
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 4303
Author(s): Kirchner,K.;Hauk,G.;Rehm,H.J.
Journal: Appl. Microbiol. Biotechnol.
Title: Exhaust gas purification using immobilised monocultures (biocatalysts).
Volume: 26
Page(s): 579-587
Year: 1987
Ref #: 4602
Author(s): DeVos,P.;Kersters,K.;Gillis,M.;Segers,P.;DeLey,J.
Journal: Int. J. Syst. Bacteriol.
Title: Comamonas Davis and Park 1962 gen. nov., nom. rev. emend., and Comamonas terrigena Hugh 1962 sp. nov., nom. rev.
Volume: 35
Page(s): 443-453
Year: 1985
Ref #: 6182
Author(s): Onken,U.
Journal: Biotechnol. Bioeng.
Title: Batch and continuous cultivation of Pseudomonas fluorescens at increased pressure.
Volume: 35
Page(s): 983-989
Year: 1990
Ref #: 7293
Author(s): Yamamoto,S.;Harayama,S.
Journal: Int. J. Syst. Bacteriol.
Title: Phylogenetic relationships of Pseudomonas putida strains deduced from the nucleotide sequences of gyrB, rpoD and 16S rRNA genes.
Volume: 48
Page(s): 813-819
Year: 1998
Ref #: 742
Title: Bergey's manual of determinative Bacteriology, 8th ed.
Year: 1974
Data: (ATCC 13525, NCIB 9046, DSM 50090, RH 818, CCB 546) Type strain / M. E. Rhodes, Reading in 1959 / Pre-filter water-works tanks in 1951 / Rhodes, M. E. (1959) J. gen. Microbiol. 21, 221 / Subcommittee (1967) Int. J. syst. Bact. 17 255
Accession Date: 01/01/1959
Authority: Migula 1895 (AL)
Depositor: RHODES M E
Taxonomy: TaxLink: S2438 (Pseudomonas fluorescens Migula 1895) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

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Ampoule (Bacteria)

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