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Bacteria Collection: Elizabethkingia meningoseptica

NCTC Number: NCTC 10016
Current Name: Elizabethkingia meningoseptica
Original Strain Reference: 14
Other Collection No: ATCC 13253; DSM 2800; IAM 14198; IFO 12535; 14
Previous Catalogue Name: Flavobacterium meningosepticum
Other Names: Chryseobacterium meningosepticum
Type Strain: Yes
Family: Flavobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar group a
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Isolated From: human, cerebrospinal fluid, premature infant
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS950464
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|D14018|IFO 12535|Flavobacterium meningosepticum 16S ribosomal RNA.| gcggcgtacgggtgc... >gb|U02896|ATCC 13253|Flavobacterium meningosepticum ATCC 13253 16S rRNA gene, partialsequence.| tgacgtcnaatcatc... >gb|AY468445|LMG 4027| ATCC13253| CIP 60.57| LMG 12279| NCTC|Chryseobacterium meningosepticum strain LMG 4027 16S ribosomal RNAgene, partial sequence.| aggttttttatctgg... >gb|AY336568|ATCC 13253|Chryseobacterium meningosepticum strain ATCC 13253 16S ribosomalRNA gene, partial sequence.| gctcaggatgaacgc...
Miscellaneous Sequence Data: >gb|AB015024|IAM 14198|Elizabethkingia meningoseptica gyrB gene for DNA gyrase subunit B,partial cds, strain:IAM 14198.| gataaagattcttat...
Bibliography: KING E O 1959 AM J CLIN PATH 31 241
Extended Bibliography: showhide Show bibliography
Ref #: 19216
Author(s): Greisen,K.;Loeffelholz,M.;Purohit,A.;Leong,D.
Journal: J Clin Microbiol
Title: PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid
Volume: 32
Page(s): 335-51
Year: 1994
Keyword(s): GENBANK/U02893 GENBANK/U02894 GENBANK/U02895 GENBANK/U02896 GENBANK/U02897 GENBANK/U02898 GENBANK/U02899 GENBANK/U02900 GENBANK/U02901 GENBANK/U02902 GENBANK/U02903 GENBANK/U02904 GENBANK/U02905 GENBANK/U02906 GENBANK/U02907 GENBANK/U02908 GENBANK/U02909 GENBANK/U02910 GENBANK/U02911 GENBANK/U02912 GENBANK/U02913 GENBANK/U02914 GENBANK/U02915 GENBANK/U02916 GENBANK/U02917 GENBANK/U02918 GENBANK/U02919 GENBANK/U02920 GENBANK/U02921 GENBANK/U02922 Bacteremia/diagnosis/microbiology Bacteria/*genetics/isolation & purification/pathogenicity Bacterial Infections/diagnosis/microbiology Base Sequence Cerebrospinal Fluid/microbiology DNA Primers/genetics DNA Probes/genetics Genes, Bacterial Gram-Negative Bacteria/genetics Gram-Positive Bacteria/genetics Humans Meningitis, Bacterial/diagnosis/microbiology Molecular Sequence Data Nucleic Acid Hybridization *Polymerase Chain Reaction/statistics & numerical data RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Sensitivity and Specificity Sequence Homology, Nucleic Acid Species Specificity
Remarks: A set of broad-range PCR primers for the 16S rRNA gene in bacteria were tested, along with three series of oligonucleotide probes to detect the PCR product. The first series of probes is broad in range and consists of a universal bacterial probe, a gram-positive probe, a Bacteroides-Flavobacterium probe, and two probes for other gram-negative species. The second series was designed to detect PCR products from seven major bacterial species or groups frequently causing meningitis: Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, S. agalactiae, Escherichia coli and other enteric bacteria, Listeria monocytogenes, and Staphylococcus aureus. The third series was designed for the detection of DNA from species or genera commonly considered potential contaminants of clinical samples, including cerebrospinal fluid (CSF): Bacillus, Corynebacterium, Propionibacterium, and coagulase-negative Staphylococcus spp. The primers amplified DNA from all 124 different species of bacteria tested. Southern hybridization testing of the broad-range probes with washes containing 3 M tetramethylammonium chloride indicated that this set of probes correctly identified all but two of the 102 bacterial species tested, the exceptions being Deinococcus radiopugnans and Gardnerella vaginalis. The gram-negative and gram-positive probes hybridized to isolates of two newly characterized bacteria, Alloiococcus otitis and Rochalimaea henselii, as predicted by Gram stain characteristics. The CSF pathogen and contaminant probe sequences were compared with available sequence information and with sequencing data for 32 different species. Testing of the CSF pathogen and contaminant probes against DNA from over 60 different strains indicated that, with the exception of the coagulase-negative Staphylococcus probes, these probes provided the correct identification of bacterial species known to be found in CSF.
URL: 7512093
Ref #: 40593
Author(s): Suzuki,M.;Nakagawa,Y.;Harayama,S.;Yamamoto,S.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis of genus Marinilabilia and related bacteria based on the amino acid sequences of gyrB and emended description of Marinilabilia salmonicolor with Marinilabilia agarovorans as its subjective synonym
Volume: 49 Pt 4
Page(s): 1551-7
Year: 1999
Keyword(s): GENBANK/AB015024 GENBANK/AB015025 GENBANK/AB015026 GENBANK/AB015027 GENBANK/AB015028 GENBANK/AB015029 GENBANK/AB015030 GENBANK/AB015031 GENBANK/AB015032 GENBANK/AB015033 GENBANK/AB015034 GENBANK/AB015035 GENBANK/AB015036 GENBANK/AB015037 GENBANK/AB015038 Amino Acid Sequence Bacteroidaceae/*classification/*genetics Bacteroides/classification/genetics Cytophaga/classification/genetics DNA Gyrase DNA Topoisomerases, Type II/*chemistry/genetics DNA, Bacterial/genetics DNA, Ribosomal/genetics Flavobacterium/classification/genetics Molecular Sequence Data Nucleic Acid Hybridization *Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA
Remarks: The detailed phylogenetic relationships for genus Marinilabilia and related taxa were analysed by using DNA gyrase B subunit gene (gyrB) sequences. Anaerobic bacteria in the Cytophaga-Flavobacterium-Bacteroides phylum, namely genera Marinilabilia, Bacteroides, Rikenella, Prevotella and Porphyromonas and Cytophaga fermentans, were clustered in the same branch and the facultative anaerobes Marinilabilia and Cytophaga fermentans formed a subcluster in the branch of the anaerobic bacteria. Phylogenetic analysis using 16S rDNA sequences gave a similar result but with a lower bootstrap value for each cluster. The gyrB sequences of Marinilabilia salmonicolor and Marinilabilia agarovorans were the same, and the relatedness of their chromosomal DNA, as determined by DNA-DNA hybridization, was greater than 70%. These genetic aspects led to the conclusion that M. salmonicolor IFO 15948T and M. agarovorans IFO 14957T belong to a single species. Since M. salmonicolor was described first, as Cytophaga salmonicolor, M. salmonicolor is a senior subjective synonym of M. agarovorans. Therefore, the name M. salmonicolor should be retained and strain IFO 14957T should be reclassified as M. salmonicolor. However, the agar-degrading ability of strain IFO 14957T is a prominent biochemical characteristic. It is therefore proposed that strain IFO 14957T should be renamed M. salmonicolor biovar agarovorans.
URL: 10555336
Ref #: 13687
Author(s): Greisen,K.;Loeffelholz,M.;Purohit,A.;Leong,D.
Journal: J Clin Microbiol
Title: PCR primers and probes for the 16S rRNA gene of most species of pathogenic
Volume: 32
Page(s): 335-351
Year: 1994
Keyword(s): 0 (DNA Primers) 0 (DNA Probes) 0 (RNA, Bacterial) 0 (RNA, Ribosomal, 16S) Bacteremia/diagnosis/microbiology Bacteria/*genetics/isolation & purification/pathogenicity Bacterial Infections/diagnosis/microbiology Base Sequence Cerebrospinal Fluid/microbiology DNA Primers/genetics DNA Probes/genetics Genes, Bacterial Gram-Negative Bacteria/genetics Gram-Positive Bacteria/genetics Human Meningitis, Bacterial/diagnosis/microbiology Molecular Sequence Data Nucleic Acid Hybridization *Polymerase Chain Reaction/statistics & numerical data RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Sensitivity and Specificity Sequence Homology, Nucleic Acid Species Specificity
Remarks: A set of broad-range PCR primers for the 16S rRNA gene in bacteria were
URL: 94201356
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 1778
Author(s): Callies,E.;Mannheim,W.
Journal: Antonie Leeuwenhoek
Title: Deoxyribonucleic acid relatedness of some menaquinone-producing Flavobacterium and Cytophaga strains.
Volume: 46
Page(s): 41-49
Year: 1980
Ref #: 1779
Author(s): Oyaizu,H.;Komagata,K.
Journal: J. Gen. Appl. Microbiol.
Title: Chemotaxonomic and phenotypic characterization of the strains of species in the Flavobacterium-Cytophaga complex.
Volume: 27
Page(s): 57-107
Year: 1981
Ref #: 2652
Author(s): King,E.O.
Journal: Am. Clin. Pathol.
Title: Studies on a group of previously unclassified bacteria associated with meningitis in infants.
Volume: 31
Page(s): 241-247
Year: 1959
Ref #: 2654
Author(s): Holmes,B.
Journal: INSERM
Title: The taxonomy of the genus Flavobacterium. In Les colloques de l'INSERM; les bacilles à Gram négatif d' intéret medical et en santé publique: taxonomie-identification-applications.
Volume: 144
Page(s): 273-294
Year: 1983
Ref #: 5198
Author(s): Vandamme,P.;Bernardet,J.-F.;Segers,P.;Kersters,K.;Holmes,B.
Journal: Int. J. Syst. Bacteriol.
Title: New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev.
Volume: 44
Page(s): 827-831
Year: 1994
Data: (ATCC 13253) Type strain / E. O. King, CDC Atlanta in 1958 / Cerebrospinal fluid, premature infant / Serotype A / King, E. O. (1959) Am. J. clin. Path. 31, 241
Accession Date: 01/01/1958
History: ISLT BRODY J A,MOORE H & KING E O,MDU,MICRO SEC,CHAMBLEE PRE:FR
Authority: (King 1959) Vandamme et al. 1994
Depositor: KING E O
Taxonomy: TaxLink: S1282 (Flavobacterium meningosepticum) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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