NCTC Number: |
NCTC 10006
|
Current Name: |
Klebsiella aerogenes
|
Original Strain Reference: |
CDC 819-56
|
Other Collection No: |
ATCC 13048; CDC 819-56; DSM 30053; WDCM 00175
|
Previous Catalogue Name: |
Enterobacter aerogenes
|
Type Strain: |
Yes
|
Family: |
Enterobacteriaceae
|
Hazard Group (ACDP): |
2
|
Release Restrictions: |
Terms & Conditions of Supply of Microbial Pathogens: Safety
|
Conditions for growth on solid media: |
Nutrient agar, 24 hours, 37°C, aerobic
|
Conditions for growth on liquid media: |
nutrient broth,37, facultative anaerobe
|
Isolated From: |
sputum
|
Whole Genome Sequence: |
http://www.ebi.ac.uk/ena/data/view/ERS462975
|
16S rRNA Gene Sequence: |
>gb|AJ001237|NCTC 10006 T|Enterobacter aerogenes 16S ribosomal RNA, partial 5 end, strainNCTC 10006 T.| cgctggcggcaggct... >gb|AJ001238|NCTC 10006 T|Enterobacter aerogenes 16S ribosomal RNA, partial 3 end, NCTC10006 T.| cgatgtcgacttgga... >gb|AJ251468|NCTC10006T|Enterobacter aerogenes partial 16S rRNA gene, strain NCTC10006T.| tgaacgctggcggca... >gb|AJ001237|NCTC 10006 T|Enterobacter aerogenes 16S ribosomal RNA, partial 5' end, strainNCTC 10006 T.| cgctggcggcaggct... >gb|AJ001238|NCTC 10006 T|Enterobacter aerogenes 16S ribosomal RNA, partial 3' end, NCTC10006 T.| cgatgtcgacttgga...
|
23S rRNA Gene Sequence: |
>gb|AY116918|ATCC 13048|Enterobacter aerogenes strain ATCC 13048 23S ribosomal RNA gene,partial sequence.| ggttaagcgactaag...
|
Miscellaneous Sequence Data: |
>gb|AJ300554|CIP 60.86T| ATCC 13048T|Enterobacter aerogenes partial gyrB gene for DNA gyrase B subunitstrain CIP 60.86T, ATCC 13048T.| ataaatttgatgata...
|
Bibliography: |
HORMAECHE E & EDWARDS P R 1960 INT BULL BACT NOMEN CL TAXON 10 71;
|
Extended Bibliography: |
Show bibliography
Ref #: |
95525 |
Author(s): |
Godfrey,V.;Logan,J.M.;Nicholson,G.;Maggs,A.F. |
Journal: |
Br J Biomed Sci |
Title: |
Problems in identifying blood culture isolates |
Volume: |
55 |
Page(s): |
12-3 |
Year: |
1998 |
Keyword(s): |
GENBANK/AJ001236
GENBANK/AJ001237
GENBANK/AJ001238
GENBANK/AJ001239
GENBANK/AJ001240
GENBANK/AJ001241
GENBANK/AJ001242
GENBANK/AJ001243
GENBANK/AJ001244
GENBANK/AJ001245
Adolescent
*Bacterial Typing Techniques
Blood/*microbiology
Female
Gram-Negative Bacteria/*classification
Humans
Phylogeny
RNA, Bacterial/genetics
Sequence Analysis, RNA
|
URL: |
9684412 |
|
Ref #: |
95492 |
Author(s): |
Fukushima,M.;Kakinuma,K.;Kawaguchi,R. |
Journal: |
J Clin Microbiol |
Title: |
Phylogenetic analysis of Salmonella, Shigella, and Escherichia coli strains on the basis of the gyrB gene sequence |
Volume: |
40 |
Page(s): |
2779-85 |
Year: |
2002 |
Keyword(s): |
DNA Gyrase/*genetics
DNA, Bacterial/analysis
DNA, Ribosomal/analysis
Escherichia coli/classification/*genetics
Humans
Molecular Sequence Data
*Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S/genetics
Salmonella/classification/*genetics
Shigella/classification/*genetics
|
Remarks: |
Phylogenetic analysis of about 200 strains of Salmonella, Shigella, and Escherichia coli was carried out using the nucleotide sequence of the gene for DNA gyrase B (gyrB), which was determined by directly sequencing PCR fragments. The results establish a new phylogenetic tree for the classification of Salmonella, Shigella, and Escherichia coli in which Salmonella forms a cluster separate from but closely related to Shigella and E. coli. In comparison with 16S rRNA analysis, the gyrB sequences indicated a greater evolutionary divergence for the bacteria. Thus, in screening for the presence of bacteria, the gyrB gene might be a useful tool for differentiating between closely related species of bacteria such as Shigella spp. and E. coli. At present, 16S rRNA sequence analysis is an accurate and rapid method for identifying most unknown bacteria to the genus level because the highly conserved 16S rRNA region is easy to amplify; however, analysis of the more variable gyrB sequence region can identify unknown bacteria to the species level. In summary, we have shown that gyrB sequence analysis is a useful alternative to 16S rRNA analysis for constructing the phylogenetic relationships of bacteria, in particular for the classification of closely related bacterial species. |
URL: |
12149329 |
|
Ref #: |
48709 |
Author(s): |
Dauga,C. |
Journal: |
Int J Syst Evol Microbiol |
Title: |
Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies |
Volume: |
52 |
Page(s): |
531-47 |
Year: |
2002 |
Keyword(s): |
GENBANK/AJ300528
GENBANK/AJ300529
GENBANK/AJ300530
GENBANK/AJ300531
GENBANK/AJ300532
GENBANK/AJ300533
GENBANK/AJ300534
GENBANK/AJ300535
GENBANK/AJ300536
GENBANK/AJ300537
GENBANK/AJ300538
GENBANK/AJ300539
GENBANK/AJ300540
GENBANK/AJ300541
GENBANK/AJ300542
GENBANK/AJ300543
GENBANK/AJ300544
GENBANK/AJ300545
GENBANK/AJ300546
GENBANK/AJ300547
GENBANK/AJ300548
GENBANK/AJ300549
GENBANK/AJ300550
GENBANK/AJ300551
GENBANK/AJ300552
GENBANK/AJ300553
GENBANK/AJ300554
DNA Gyrase/*genetics
Enterobacteriaceae/*classification/genetics
Evolution, Molecular
Genes, rRNA
Molecular Sequence Data
Phenotype
RNA, Bacterial/chemistry
RNA, Ribosomal, 16S/chemistry
|
Remarks: |
Phylogenetic trees showing the evolutionary relatedness of Enterobacteriaceae based upon gyrB and 16S rRNA genes were compared. Congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. Phylogenetic trees based on gyrB sequences appeared to be more reliable at determining relationships among Serratia species than trees based on 16S rRNA gene sequences. gyrB sequences from Serratia species formed a monophyletic group validated by significant bootstrap values. Serratia fonticola had the most deeply branching gyrB sequence in the Serratia monophyletic group, which was consistent with its atypical phenotypic characteristics. Klebsiella and Enterobacter genera seemed to be polyphyletic, but the branching patterns of gyrB and 16S rRNA gene trees were not congruent. Enterobacter aerogenes was grouped with Klebsiella pneumoniae on the gyrB phylogenetic tree, which supports that this species could be transferred to the Klebsiella genus. Unfortunately, 16S rRNA and gyrB phylogenetic trees gave conflicting evolutionary relationships for Citrobacter freundii because of its unusual gyrB evolutionary process. gyrB lateral gene transfer was suspected for Hafnia alvei. Saturation of gyrB genes was observed by the pairwise comparison of Proteus spp., Providencia alcalifaciens and Morganella morganii sequences. Depending on their level of variability, 16S rRNA gene sequences were useful for describing phylogenetic relationships between distantly related Enterobacteriaceae, whereas gyrB sequence comparison was useful for inferring intra- and some intergeneric relationships. |
URL: |
11931166 |
|
Ref #: |
12648 |
Author(s): |
Godfrey,V.;Logan,J.M.;Nicholson,G.;Maggs,A.F. |
Journal: |
Br J Biomed Sci |
Title: |
Problems in identifying blood culture isolates |
Volume: |
55 |
Page(s): |
12-3 |
Year: |
1998 |
Keyword(s): |
GENBANK/AJ001236
GENBANK/AJ001237
GENBANK/AJ001238
GENBANK/AJ001239
GENBANK/AJ001240
GENBANK/AJ001241
GENBANK/AJ001242
GENBANK/AJ001243
GENBANK/AJ001244
GENBANK/AJ001245
Adolescent
*Bacterial Typing Techniques
Blood/*microbiology
Case Report
Female
Gram-Negative Bacteria/*classification
Human
Phylogeny
RNA, Bacterial/genetics
Sequence Analysis, RNA
Support, Non-U.S. Gov't
|
URL: |
98349037 |
|
Ref #: |
1300 |
Author(s): |
Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed) |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Approved Lists of Bacterial Names. |
Volume: |
30 |
Page(s): |
225-420 |
Year: |
1980 |
|
Ref #: |
4300 |
Author(s): |
Jagnow,G.;Haider,K.;Ellwardt,P.-C. |
Journal: |
Arch. Microbiol. |
Title: |
Anaerobic dechlorination and degradation of hexachlorocyclohexane isomers by anaerobic and facultative anaerobic bacteria. |
Volume: |
115 |
Page(s): |
285-292 |
Year: |
1977 |
|
Ref #: |
6924 |
Author(s): |
DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed) |
Title: |
DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme. |
Year: |
1997 |
|
|
Data: |
(Cloaca B, ATCC 13048) Type strain / P. R. Edwards, CDC Atlanta in 1958 / Sputum / Hormaeche, E. & Edwards, P. R. (1958) Int. Bull. bact. Nomencl. Taxon. 8, 111 and (1960) 10, 71
|
Accession Date: |
01/01/1958
|
History: |
EDWARDS P R CDC ATLANTA
|
Authority: |
(Hormaeche and Edwards 1960) Tindall et al. 2017
|
Depositor: |
EDWARDS P R
|
Taxonomy: |
TaxLink: S1128 (Enterobacter aerogenes Hormaeche and Edwards 1960) - Date of change: 5/02/2003
|
Biosafety Responsibility: |
It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country
|
The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.
Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.