Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Enterobacter cloacae

NCTC Number: NCTC 10005
Current Name: Enterobacter cloacae
Original Strain Reference: 279-56
Other Collection No: ATCC 13047; CIP 60.85; DSM 30054; IFO 13535; NCDC 279-56; WDCM 00083
Previous Catalogue Name: Enterobacter cloacae
Type Strain: Yes
Family: Enterobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, cerebrospinal fluid
Whole Genome Sequence:
Annotated Genome:
16S rRNA Gene Sequence: >gb|AJ001245|NCTC 10005 T|Enterobacter cloacae 16S ribosomal RNA, partial 5 end, strain NCTC10005 T.| cgctggcggcaggct... >gb|AJ001236|NCTC 10005 T|Enterobacter cloacae 16S ribosomal RNA, partial 3 end, strain NCTC10005 T.| ttcgatgtcgatttg... >gb|AJ417484|ATCC 13047T|Enterobacter cloacae partial 16S rRNA gene, strain ATCC 13047T.| gtagcacagagagct... >gb|AJ251469|ATCC13047T|Enterobacter cloacae partial 16S rRNA gene, strain ATCC13047T.| tgaacgctggcggca... >gb|U65720|ATCC 13047|Enterobacter cloacae 16S ribosomal RNA gene, partial sequence.| catgatgtcgacaga... >gb|AJ417484|TYPE STRAIN: ATCC 13047|Enterobacter cloacae partial 16S rRNA gene, strain ATCC 13047T.| gtagcacagagagct... >gb|AJ001245|NCTC 10005 T|Enterobacter cloacae 16S ribosomal RNA, partial 5' end, strain NCTC10005 T.| cgctggcggcaggct... >gb|AJ001236|NCTC 10005 T|Enterobacter cloacae 16S ribosomal RNA, partial 3' end, strain NCTC10005 T.| ttcgatgtcgatttg...
23S rRNA Gene Sequence: >gb|AJ549515|TYPE STRAIN: DSM 30054|Enterobacter cloacae partial 23S rRNA gene, type strain DSM 30054T.| catttaaagaaagcg...
Miscellaneous Sequence Data: >gb|AJ300553|CIP 60.85T| ATCC 13047T|Enterobacter cloacae partial gyrB gene for DNA gyrase B subunitstrain CIP 60.85T, ATCC 13047T.| ataaatttgacgata...
Extended Bibliography: showhide Show bibliography
Ref #: 95525
Author(s): Godfrey,V.;Logan,J.M.;Nicholson,G.;Maggs,A.F.
Journal: Br J Biomed Sci
Title: Problems in identifying blood culture isolates
Volume: 55
Page(s): 12-3
Year: 1998
Keyword(s): GENBANK/AJ001236 GENBANK/AJ001237 GENBANK/AJ001238 GENBANK/AJ001239 GENBANK/AJ001240 GENBANK/AJ001241 GENBANK/AJ001242 GENBANK/AJ001243 GENBANK/AJ001244 GENBANK/AJ001245 Adolescent *Bacterial Typing Techniques Blood/*microbiology Female Gram-Negative Bacteria/*classification Humans Phylogeny RNA, Bacterial/genetics Sequence Analysis, RNA
URL: 9684412
Ref #: 95492
Author(s): Fukushima,M.;Kakinuma,K.;Kawaguchi,R.
Journal: J Clin Microbiol
Title: Phylogenetic analysis of Salmonella, Shigella, and Escherichia coli strains on the basis of the gyrB gene sequence
Volume: 40
Page(s): 2779-85
Year: 2002
Keyword(s): DNA Gyrase/*genetics DNA, Bacterial/analysis DNA, Ribosomal/analysis Escherichia coli/classification/*genetics Humans Molecular Sequence Data *Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Salmonella/classification/*genetics Shigella/classification/*genetics
Remarks: Phylogenetic analysis of about 200 strains of Salmonella, Shigella, and Escherichia coli was carried out using the nucleotide sequence of the gene for DNA gyrase B (gyrB), which was determined by directly sequencing PCR fragments. The results establish a new phylogenetic tree for the classification of Salmonella, Shigella, and Escherichia coli in which Salmonella forms a cluster separate from but closely related to Shigella and E. coli. In comparison with 16S rRNA analysis, the gyrB sequences indicated a greater evolutionary divergence for the bacteria. Thus, in screening for the presence of bacteria, the gyrB gene might be a useful tool for differentiating between closely related species of bacteria such as Shigella spp. and E. coli. At present, 16S rRNA sequence analysis is an accurate and rapid method for identifying most unknown bacteria to the genus level because the highly conserved 16S rRNA region is easy to amplify; however, analysis of the more variable gyrB sequence region can identify unknown bacteria to the species level. In summary, we have shown that gyrB sequence analysis is a useful alternative to 16S rRNA analysis for constructing the phylogenetic relationships of bacteria, in particular for the classification of closely related bacterial species.
URL: 12149329
Ref #: 43062
Author(s): Hoffmann,H.;Roggenkamp,A.
Journal: Appl Environ Microbiol
Title: Population genetics of the nomenspecies Enterobacter cloacae
Volume: 69
Page(s): 5306-18
Year: 2003
Keyword(s): Anti-Bacterial Agents/pharmacology Base Sequence Chaperonin 60/genetics DNA Primers DNA, Bacterial/genetics Enterobacter cloacae/*classification/drug effects/*genetics/isolation & purification Humans Microbial Sensitivity Tests Multigene Family Nucleic Acid Hybridization *Phylogeny Polymerase Chain Reaction/methods *Polymorphism, Restriction Fragment Length
Remarks: The genetic heterogeneity of the nomenspecies Enterobacter cloacae is well known. Enterobacter asburiae, Enterobacter cancerogenus, Enterobacter dissolvens, Enterobacter hormaechei, Enterobacter kobei, and Enterobacter nimipressuralis are closely related to it and are subsumed in the so-called E. cloacae complex. DNA-DNA hybridization studies performed previously identified at least five DNA-relatedness groups of this complex. In order to analyze the genetic structure and the phylogenetic relationships between the clusters of the nomenspecies E. cloacae, 206 strains collected from 22 hospitals, a veterinarian, and an agricultural center in 11 countries plus all 13 type strains of the genus and reference strain CDC 1347-71(R) were examined with a combination of sequence and PCR-restriction fragment length polymorphism (PCR-RFLP) analyses of the three housekeeping genes hsp60, rpoB, and hemB as well as ampC, the gene of a class C beta-lactamase. Based on the neighbor-joining tree of the hsp60 sequences, 12 genetic clusters (I to XII) and an unstable sequence crowd (xiii) were identified. The robustness of the genetic clusters was confirmed by analyses of rpoB and hemB sequences and ampC PCR-RFLPs. Sequence crowd xiii split into two groups after rpoB analysis. Only three strains formed a cluster with the type strain of E. cloacae, indicating that the minority of isolates identified as E. cloacae truly belong to the species; 13% of strains grouped with other type strains of the genus, suggesting that the phenotypes of these species seem to be more heterogeneous than so far believed. Three clusters represented 70% of strains, but none of them included a type or reference strain. The genetic clustering presented in this study might serve as a framework for future studies dealing with taxonomic, evolutionary, epidemiological, or pathogenetic characteristics of bacteria belonging to the E. cloacae complex.
URL: 12957918
Ref #: 95479
Author(s): Mitterer,G.;Huber,M.;Leidinger,E.;Kirisits,C.;Lubitz,W.;Mueller,M.W.;Schmidt,W.M.
Journal: J Clin Microbiol
Title: Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences
Volume: 42
Page(s): 1048-57
Year: 2004
Keyword(s): Abortion, Veterinary/microbiology Animals Bacteria/*genetics/*isolation & purification Base Sequence DNA Primers DNA, Bacterial/genetics/isolation & purification DNA, Ribosomal/*genetics/isolation & purification Female Horses *Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction/*methods Pregnancy Pregnancy Complications, Infectious/microbiology/veterinary RNA, Bacterial/genetics/isolation & purification RNA, Ribosomal, 23S/*genetics/isolation & purification
Remarks: The rapid identification of the bacteria in clinical samples is important for patient management and antimicrobial therapy. We describe a DNA microarray-based PCR approach for the quick detection and identification of bacteria from cervical swab specimens from mares. This on-chip PCR method combines the amplification of a variable region of bacterial 23S ribosomal DNA and the simultaneous sequence-specific detection on a solid phase. The solid phase contains bacterial species-specific primers covalently bound to a glass support. During the solid-phase amplification reaction the polymerase elongates perfectly matched primers and incorporates biotin-labeled nucleotides. The reaction products are visualized by streptavidin-cyanine 5 staining, followed by fluorescence scanning. This procedure successfully identified from pure cultures 22 bacteria that are common causes of abortion and sterility in mares. Using the on-chip PCR method, we also tested 21 cervical swab specimens from mares for the presence of pathogenic bacteria and compared the results with those of conventional bacteriological culture methods. Our method correctly identified the bacteria in 12 cervical swab samples, 8 of which contained more than one bacterial species. Due to the higher sensitivity of the on-chip PCR, this method identified bacteria in five cervical swab samples which were not detected by the conventional identification procedure. Our results show that this method will have great potential to be incorporated into the routine microbiology laboratory.
URL: 15004052
Ref #: 48709
Author(s): Dauga,C.
Journal: Int J Syst Evol Microbiol
Title: Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies
Volume: 52
Page(s): 531-47
Year: 2002
Keyword(s): GENBANK/AJ300528 GENBANK/AJ300529 GENBANK/AJ300530 GENBANK/AJ300531 GENBANK/AJ300532 GENBANK/AJ300533 GENBANK/AJ300534 GENBANK/AJ300535 GENBANK/AJ300536 GENBANK/AJ300537 GENBANK/AJ300538 GENBANK/AJ300539 GENBANK/AJ300540 GENBANK/AJ300541 GENBANK/AJ300542 GENBANK/AJ300543 GENBANK/AJ300544 GENBANK/AJ300545 GENBANK/AJ300546 GENBANK/AJ300547 GENBANK/AJ300548 GENBANK/AJ300549 GENBANK/AJ300550 GENBANK/AJ300551 GENBANK/AJ300552 GENBANK/AJ300553 GENBANK/AJ300554 DNA Gyrase/*genetics Enterobacteriaceae/*classification/genetics Evolution, Molecular Genes, rRNA Molecular Sequence Data Phenotype RNA, Bacterial/chemistry RNA, Ribosomal, 16S/chemistry
Remarks: Phylogenetic trees showing the evolutionary relatedness of Enterobacteriaceae based upon gyrB and 16S rRNA genes were compared. Congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. Phylogenetic trees based on gyrB sequences appeared to be more reliable at determining relationships among Serratia species than trees based on 16S rRNA gene sequences. gyrB sequences from Serratia species formed a monophyletic group validated by significant bootstrap values. Serratia fonticola had the most deeply branching gyrB sequence in the Serratia monophyletic group, which was consistent with its atypical phenotypic characteristics. Klebsiella and Enterobacter genera seemed to be polyphyletic, but the branching patterns of gyrB and 16S rRNA gene trees were not congruent. Enterobacter aerogenes was grouped with Klebsiella pneumoniae on the gyrB phylogenetic tree, which supports that this species could be transferred to the Klebsiella genus. Unfortunately, 16S rRNA and gyrB phylogenetic trees gave conflicting evolutionary relationships for Citrobacter freundii because of its unusual gyrB evolutionary process. gyrB lateral gene transfer was suspected for Hafnia alvei. Saturation of gyrB genes was observed by the pairwise comparison of Proteus spp., Providencia alcalifaciens and Morganella morganii sequences. Depending on their level of variability, 16S rRNA gene sequences were useful for describing phylogenetic relationships between distantly related Enterobacteriaceae, whereas gyrB sequence comparison was useful for inferring intra- and some intergeneric relationships.
URL: 11931166
Ref #: 12648
Author(s): Godfrey,V.;Logan,J.M.;Nicholson,G.;Maggs,A.F.
Journal: Br J Biomed Sci
Title: Problems in identifying blood culture isolates
Volume: 55
Page(s): 12-3
Year: 1998
Keyword(s): GENBANK/AJ001236 GENBANK/AJ001237 GENBANK/AJ001238 GENBANK/AJ001239 GENBANK/AJ001240 GENBANK/AJ001241 GENBANK/AJ001242 GENBANK/AJ001243 GENBANK/AJ001244 GENBANK/AJ001245 Adolescent *Bacterial Typing Techniques Blood/*microbiology Case Report Female Gram-Negative Bacteria/*classification Human Phylogeny RNA, Bacterial/genetics Sequence Analysis, RNA Support, Non-U.S. Gov't
URL: 98349037
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 3825
Author(s): Izard,D.;Gavini,F.;Leclerc,H.
Journal: Zbl. Bakt. Hyg., I. Abt. Orig. C
Title: Polynucleotide sequence relatedness and genome size among Enterobacter intermedius sp. nov. and the species Enterobacter cloacae and Klebsiella pneumoniae.
Volume: 1
Page(s): 57
Year: 1980
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Data: (Cloaca A, ATCC 13047) Type strain / P.R. Edwards, CDC Atlanta in 1958 / Cerebrospinal fluid / Hormaeche, E. & Edwards, P. R. (1958) Int. Bull. bact. Nomencl. Taxon. 8, 111 and (1960) 10, 71
Accession Date: 01/01/1958
Authority: (Jordan 1890) Hormaeche and Edwards 1960 (AL)
Depositor: EDWARDS P R
Taxonomy: TaxLink: S9327 (Enterobacter cloacae subspecies cloacae (jordan 1890) hormaeche and edwards 1960) - Date of change: 16/06/2007 by NCTCUp to 16/06/2007: S1133 (Enterobacter cloacae (Jordan 1890) Hormaeche and Edwards 1960) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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