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Bacteria Collection: Clostridium putrefaciens

NCTC Number: NCTC 9836
Current Name: Clostridium putrefaciens
Original Strain Reference: Barnes
Other Collection No: ATCC 25486; ATCC 25786; BARNES; DSM 1291; VPI 5395
Previous Catalogue Name: Clostridium putrefaciens
Type Strain: Yes
Family: Clostridiaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: 3% neo-peptone agar,22, strict anaerobe
Conditions for growth on liquid media: meat broth,22, strict anaerobe
Isolated From: ham
16S rRNA Gene Sequence: >gb|AF127024|DSM 1291|Clostridium putrefaciens 16S ribosomal RNA gene, partial sequence.| agagtttgatcctgg... >gb|Y18177|DSM 1291|Clostridium putrifaciens 16S rRNA gene, partial, strain DSM 1291.| ggctcaggacgaacg...
Bibliography: ROSS H E 1975 J APPL BACT 28 49;STURGES W S & DRAKE E T 1927 J BACT 14 175
Extended Bibliography: showhide Show bibliography
Ref #: 13875
Author(s): Stackebrandt,E.;Kramer,I.;Swiderski,J.;Hippe,H.
Journal: FEMS Immunol Med Microbiol
Title: Phylogenetic basis for a taxonomic dissection of the genus Clostridium
Volume: 24
Page(s): 253-8
Year: 1999
Keyword(s): GENBANK/Y18170 GENBANK/Y18171 GENBANK/Y18172 GENBANK/Y18173 GENBANK/Y18174 GENBANK/Y18175 GENBANK/Y18176 GENBANK/Y18177 GENBANK/Y18178 GENBANK/Y18179 GENBANK/Y18180 GENBANK/Y18181 GENBANK/Y18182 GENBANK/Y18183 GENBANK/Y18184 GENBANK/Y18185 GENBANK/Y18186 GENBANK/Y18187 GENBANK/Y18188 GENBANK/Y18189 GENBANK/Y18190 GENBANK/Y18191 Base Sequence Classification Clostridium/*classification/genetics DNA, Bacterial/analysis DNA, Ribosomal/analysis Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/analysis
Remarks: The 16S rDNA-based phylogenetic analysis of the genus Clostridium has been completed by determination of the phylogenetic position of the type strains of 15 species and two non-validated species. These strains are members of phylogenetic clusters I, III, IV, V, IX, XIVa and XVIII as defined previously by Collins et al. [Int. J. Syst. Bacteriol. 44 (1994) 812-826]. Members of the genus Clostridium span a large evolutionary distance and the genus is not a phylogenetically coherent taxon but is intermixed with members of different genera, exhibiting a combination of Clostridium- and non-Clostridium-type properties. Anaerobacter polyendosporus, Syntrophococcus sucromutans and Acetivibrio multivorans also cluster within the radiation of Clostridium species. Although several taxa have been described for former Clostridium species with distinct phenotypic properties, the majority of Clostridium species, which are not members of the core cluster I, can at present not be reclassified as long as taxon-specific, phenotypic properties are not available.
URL: 10397308
Ref #: 95507
Author(s): Broda,D.M.;Musgrave,D.R.;Bell,R.G.
Journal: J Appl Microbiol
Title: Use of restriction fragment length polymorphism analysis to differentiate strains of psychrophilic and psychrotropic clostridia associated with blown pack' spoilage of vacuum-packed meats
Volume: 88
Page(s): 107-16
Year: 2000
Keyword(s): Clostridium/*classification/genetics/growth & development *Cold DNA, Bacterial/analysis DNA, Ribosomal/analysis *Food Microbiology Food Packaging Meat Products/*microbiology Polymerase Chain Reaction Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S/analysis Sequence Alignment Sequence Analysis, DNA Vacuum
Remarks: Reference and meat strains of psychrophilic and psychrotrophic clostridia were differentiated using restriction fragment length polymorphism (RFLP) analysis of genomic DNA (DNA-RFLP) and the polymerase chain reaction-amplified 16S rDNA gene (PCR-RFLP). Groupings obtained with PCR-RFLP were confirmed with 16S rDNA gene sequencing. DNA-RFLP resolved 19 of the 22 meat strains into 11 groups. Three meat strains were untypable using this method. All reference strains representing different genotypic species could be distinguished by the restriction patterns of 16S rDNA genes. With PCR-RFLP, the 22 meat strains produced eight distinct genotypes. 16S rDNA gene sequencing confirmed that each genotype was represented by a distinct sequence. PCR-RFLP restriction patterns of 15 meat strains matched those of one of two of the seven reference strains used. Seven meat strains whose RFLP restriction patterns of 16S rDNA genes differed from those of any reference strains probably represent four previously undescribed species. Although RFLP analysis of the amplified 16S rDNA gene allowed differentiation of psychrophilic and psychrotrophic clostridia at the genotypic species level and below, comparison of PCR-RFLP patterns and 16S rDNA sequences of unknown clostridial isolates with patterns and sequences of reference strains may not effect ready identification of these micro-organisms. The results of this study will be useful in diagnosis of the cause of premature spoilage of chilled vacuum-packed meats and in tracing spoilage-causing clostridia to their source(s) in the abattoir.
URL: 10735249
Ref #: 12099
Author(s): Stackebrandt,E.;Kramer,I.;Swiderski,J.;Hippe,H.
Journal: FEMS Immunol Med Microbiol
Title: Phylogenetic basis for a taxonomic dissection of the genus Clostridium
Volume: 24
Page(s): 253-8
Year: 1999
Keyword(s): GENBANK/Y18170 GENBANK/Y18171 GENBANK/Y18172 GENBANK/Y18173 GENBANK/Y18174 GENBANK/Y18175 GENBANK/Y18176 GENBANK/Y18177 GENBANK/Y18178 GENBANK/Y18179 GENBANK/Y18180 GENBANK/Y18181 GENBANK/Y18182 GENBANK/Y18183 GENBANK/Y18184 GENBANK/Y18185 GENBANK/Y18186 GENBANK/Y18187 GENBANK/Y18188 GENBANK/Y18189 GENBANK/Y18190 GENBANK/Y18191 Base Sequence Classification Clostridium/*classification/genetics DNA, Bacterial/analysis DNA, Ribosomal/analysis Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/analysis
Remarks: The 16S rDNA-based phylogenetic analysis of the genus Clostridium has been completed by determination of the phylogenetic position of the type strains of 15 species and two non-validated species. These strains are members of phylogenetic clusters I, III, IV, V, IX, XIVa and XVIII as defined previously by Collins et al. [Int. J. Syst. Bacteriol. 44 (1994) 812-826]. Members of the genus Clostridium span a large evolutionary distance and the genus is not a phylogenetically coherent taxon but is intermixed with members of different genera, exhibiting a combination of Clostridium- and non-Clostridium-type properties. Anaerobacter polyendosporus, Syntrophococcus sucromutans and Acetivibrio multivorans also cluster within the radiation of Clostridium species. Although several taxa have been described for former Clostridium species with distinct phenotypic properties, the majority of Clostridium species, which are not members of the core cluster I, can at present not be reclassified as long as taxon-specific, phenotypic properties are not available.
URL: 99323437
Ref #: 12433
Author(s): Broda,D.M.;Musgrave,D.R.;Bell,R.G.
Journal: J Appl Microbiol
Title: Use of restriction fragment length polymorphism analysis to differentiate strains of psychrophilic and psychrotropic clostridia associated with blown pack' spoilage of vacuum-packed meats
Volume: 88
Page(s): 107-16
Year: 2000
Keyword(s): Clostridium/*classification/genetics/growth & development *Cold DNA, Bacterial/analysis DNA, Ribosomal/analysis *Food Microbiology Food Packaging Meat Products/*microbiology Polymerase Chain Reaction Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S/analysis Sequence Alignment Sequence Analysis, DNA Support, Non-U.S. Gov't Vacuum
Remarks: Reference and meat strains of psychrophilic and psychrotrophic clostridia were differentiated using restriction fragment length polymorphism (RFLP) analysis of genomic DNA (DNA-RFLP) and the polymerase chain reaction-amplified 16S rDNA gene (PCR-RFLP). Groupings obtained with PCR-RFLP were confirmed with 16S rDNA gene sequencing. DNA-RFLP resolved 19 of the 22 meat strains into 11 groups. Three meat strains were untypable using this method. All reference strains representing different genotypic species could be distinguished by the restriction patterns of 16S rDNA genes. With PCR-RFLP, the 22 meat strains produced eight distinct genotypes. 16S rDNA gene sequencing confirmed that each genotype was represented by a distinct sequence. PCR-RFLP restriction patterns of 15 meat strains matched those of one of two of the seven reference strains used. Seven meat strains whose RFLP restriction patterns of 16S rDNA genes differed from those of any reference strains probably represent four previously undescribed species. Although RFLP analysis of the amplified 16S rDNA gene allowed differentiation of psychrophilic and psychrotrophic clostridia at the genotypic species level and below, comparison of PCR-RFLP patterns and 16S rDNA sequences of unknown clostridial isolates with patterns and sequences of reference strains may not effect ready identification of these micro-organisms. The results of this study will be useful in diagnosis of the cause of premature spoilage of chilled vacuum-packed meats and in tracing spoilage-causing clostridia to their source(s) in the abattoir.
URL: 20197296
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 2130
Author(s): Ross,H.E.
Journal: J. Appl. Bacteriol.
Title: Clostridium putrefaciens: a neglected anaerobe.
Volume: 28
Page(s): 49-51
Year: 1965
Ref #: 2131
Author(s): Roberts,T.A.;Derrik,C.M.
Journal: J. Appl. Bacteriol.
Title: Sporulation of Clostridium putrefaciens and the resistance of the spores to heat, gamma-radiation and curing salts.
Volume: 38
Page(s): 33-37
Year: 1975
Ref #: 2132
Author(s): Roberts,T.A.;Hobbs,G.
Journal: J. Appl. Bacteriol.
Title: Low temperature growth characteristics of clostridia.
Volume: 31
Page(s): 75-88
Year: 1968
Ref #: 2196
Author(s): Mead,G.C.
Journal: J. Gen. Microbiol.
Title: The amino acid-fermenting clostridia.
Volume: 67
Page(s): 47-56
Year: 1971
Ref #: 2197
Author(s): Elsden,S.R.;Hilton,M.G.;Waller,J.M.
Journal: Arch. Microbiol.
Title: The end products of the metabolism of aromatic amino acids by clostridia.
Volume: 107
Page(s): 283-288
Year: 1976
Ref #: 2199
Author(s): Elsden,S.R.;Hilton,M.G.;Parsley,K.R.;Self,R.
Journal: J. Gen. Microbiol.
Title: The lipid fatty acids of proteolytic clostridia.
Volume: 118
Page(s): 115-123
Year: 1980
Data: (ATCC 25786, DSM 1291) Type strain / E. M. Barnes, Cambridge in 1956 / Ham / Sturges, W. S. & Drake, E. T. (1927) J. Bact. 14, 175 / Ross, H. E. (1965) J. appl. Bact. 28, 49
Accession Date: 01/01/1956
History: ISOLATED BY BARNES E M CAMBRIDGE
Authority: (McBryde 1911) Sturges and Drake 1927 (AL)
Depositor: BARNES E M
Taxonomy: TaxLink: S891 (Clostridium putrefaciens (McBryde 1911) Sturges and Drake 1927) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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