| Extended Bibliography: |
Show bibliography
| Ref #: |
95538 |
| Author(s): |
Ruimy,R.;Podglajen,I.;Breuil,J.;Christen,R.;Collatz,E. |
| Journal: |
J Bacteriol |
| Title: |
A recent fixation of cfiA genes in a monophyletic cluster of Bacteroides fragilis is correlated with the presence of multiple insertion elements |
| Volume: |
178 |
| Page(s): |
1914-8 |
| Year: |
1996 |
| Keyword(s): |
GENBANK/X83935
GENBANK/X83936
GENBANK/X83937
GENBANK/X83938
GENBANK/X83939
GENBANK/X83940
GENBANK/X83941
GENBANK/X83942
GENBANK/X83943
GENBANK/X83944
GENBANK/X83945
GENBANK/X83946
GENBANK/X83947
GENBANK/X83948
GENBANK/X83949
GENBANK/X83950
GENBANK/X83951
GENBANK/X83952
GENBANK/X83953
GENBANK/X83954
*Bacterial Proteins
Bacteroides fragilis/*classification/*genetics
Base Sequence
*DNA Transposable Elements
DNA, Bacterial
DNA, Ribosomal
Flavobacterium/classification/genetics
Flexibacteraceae/classification/genetics
*Genes, Bacterial
Molecular Sequence Data
Multigene Family
Phylogeny
beta-Lactamases/classification/*genetics
|
| Remarks: |
Small-subunit ribosomal DNA sequences of 16 strains of Bacteroides fragilis were determined and compared with previously published sequences. Three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods) as well as a bootstrap analysis were used to assess the robustness of each topology. All phylogenetic analyses demonstrated that the B. fragilis strains were clearly divided into two robust monophyletic units which corresponded to the cfiA-negative and cfiA-positive groups. Strains of two previously identified DNA homology groups separated similarly into the two monophyletic units. According to the intensity of the hybridization signal with a cfiA probe, the cfiA-positive cluster could be further divided into two groups. This difference might reflect the existence of two, probably closely related cfiA-type genes. In the strongly hybridizing cfiA-positive strains, the gene is capable of conferring high-level resistance to the carbapenems and to most beta-lactamase inhibitors as well, while in the weakly hybridizing cfiA-positive strains, only the latter type of resistance is known to occur. The presence of the cfiA-type genes within a monophyletic cluster of B. fragilis that apparently represents only a minority of the species B. fragilis is suggestive of a recent acquisition. The fact that this cluster is also the predominant pool of all known B. fragilis insertion elements, which have been found to play an important role in the expression of carbapenem resistance, raises the possibility that both genetic determinants, i.e., the resistance gene(s) and insertion elements, may have coevolved. |
| URL: |
8606165 |
|
| Ref #: |
25269 |
| Author(s): |
Liu,C.;Song,Y.;McTeague,M.;Vu,A.W.;Wexler,H.;Finegold,S.M. |
| Journal: |
FEMS Microbiol Lett |
| Title: |
Rapid identification of the species of the Bacteroides fragilis group by multiplex PCR assays using group- and species-specific primers |
| Volume: |
222 |
| Page(s): |
9-16 |
| Year: |
2003 |
| Keyword(s): |
Bacteriological Techniques
Bacteroides fragilis/*classification/*genetics/growth & development
DNA Transposable Elements/genetics
Phenotype
Polymerase Chain Reaction/*methods
RNA, Ribosomal, 16S/genetics
RNA, Ribosomal, 23S/genetics
|
| Remarks: |
We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were first divided into three subgroups by multiplex PCR-G, followed by three multiplex PCR assays with three species-specific primer mixtures for identification to the species level. The primers were designed from nucleotide sequences of the 16S rRNA, the 16S-23S rRNA intergenic spacer region and part of the 23S rRNA gene. The established two-step multiplex PCR identification scheme was applied to the identification of 155 clinical isolates of the B. fragilis group that were previously identified to the species level by phenotypic tests. The new scheme was more accurate than phenotypic identification, which was accurate only 84.5% of the time. The multiplex PCR scheme established in this study is a simple, rapid and reliable method for the identification of the B. fragilis group species. This will permit more accurate assessment of the role of various B. fragilis group members in infections and of the degree of antimicrobial resistance in each of the group members. |
| URL: |
12757940 |
|
| Ref #: |
25883 |
| Author(s): |
Matsuo,Y.;Suzuki,M.;Kasai,H.;Shizuri,Y.;Harayama,S. |
| Journal: |
Environ Microbiol |
| Title: |
Isolation and phylogenetic characterization of bacteria capable of inducing differentiation in the green alga Monostroma oxyspermum |
| Volume: |
5 |
| Page(s): |
25-35 |
| Year: |
2003 |
| Keyword(s): |
GENBANK/AB073026
GENBANK/AB073027
GENBANK/AB073028
GENBANK/AB073029
GENBANK/AB073030
GENBANK/AB073031
GENBANK/AB073032
GENBANK/AB073033
GENBANK/AB073034
GENBANK/AB073035
GENBANK/AB073036
GENBANK/AB073037
GENBANK/AB073038
GENBANK/AB073039
GENBANK/AB073040
GENBANK/AB073041
GENBANK/AB073042
GENBANK/AB073043
GENBANK/AB073044
GENBANK/AB073045
GENBANK/AB073046
GENBANK/AB073047
GENBANK/AB073048
GENBANK/AB073049
GENBANK/AB073050
GENBANK/AB073051
GENBANK/AB073052
GENBANK/AB073053
GENBANK/AB073054
GENBANK/AB073055
GENBANK/AB073056
GENBANK/AB073057
GENBANK/AB073058
GENBANK/AB073059
GENBANK/AB073060
GENBANK/AB073061
GENBANK/AB073062
GENBANK/AB073063
GENBANK/AB073064
GENBANK/AB073065
GENBANK/AB073066
GENBANK/AB073067
GENBANK/AB073068
GENBANK/AB073069
GENBANK/AB073070
GENBANK/AB073071
GENBANK/AB073072
GENBANK/AB073073
GENBANK/AB073074
GENBANK/AB073075
GENBANK/AB073076
GENBANK/AB073077
GENBANK/AB073078
GENBANK/AB073079
GENBANK/AB073080
GENBANK/AB073081
GENBANK/AB073082
GENBANK/AB073083
GENBANK/AB073084
GENBANK/AB073085
GENBANK/AB073086
GENBANK/AB073087
GENBANK/AB073088
GENBANK/AB073089
GENBANK/AB073090
GENBANK/AB073091
GENBANK/AB073092
GENBANK/AB073093
GENBANK/AB073094
GENBANK/AB073095
GENBANK/AB073096
GENBANK/AB073097
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GENBANK/AB073110
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GENBANK/AB073586
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Algae/microbiology
Algae, Green/*growth & development/microbiology
Bacteroides/genetics/growth & development/*isolation & purification
Culture Media
Cytophaga/genetics/growth & development/*isolation & purification
DNA Gyrase/genetics
DNA, Ribosomal/analysis
Flavobacterium/genetics/growth & development/*isolation & purification
Molecular Sequence Data
Morphogenesis
*Phylogeny
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
|
| Remarks: |
Many green algae cannot develop normally when they are grown under axenic conditions. Monostroma oxyspermum, for example, proliferates unicellularly in an aseptic culture, but develops into a normal foliaceous gametophyte in the presence of some marine bacteria. More than 1000 bacterial strains were isolated from marine algae and sponges and assayed for their ability to induce the morphogenesis of unicellular M. oxyspermum. Fifty bacterial strains exhibiting morphogenesis-inducing activity against unicellular M. oxyspermum were isolated. The partial gyrB (approximately 1.2 kbp) and 16S rDNA (approximately 1.4 kbp) sequences of about 40 active strains were determined, and their phylogenetic relationships were analysed. All these strains were located within the Cytophaga-Flavobacterium-Bacteroides (CFB) complex, and most of these strains were clustered in a clade comprising Zobellia uliginosa. On the other hand, these bacteria also exhibited morphogenetic activity against germ-free spores of Ulva pertusa, Ulva conglobata and Enteromorpha intestinalis. Moreover, these bacteria induced the release of spores from the leafy young gametophyte of M. oxyspermum. These results indicate that strains belonging to several groups in the CFB complex play an important role in the normal development of green algae in the marine coastal environment. |
| URL: |
12542710 |
|
| Ref #: |
12216 |
| Author(s): |
Ruimy,R.;Podglajen,I.;Breuil,J.;Christen,R.;Collatz,E. |
| Journal: |
J Bacteriol |
| Title: |
A recent fixation of cfiA genes in a monophyletic cluster of Bacteroides fragilis is correlated with the presence of multiple insertion elements |
| Volume: |
178 |
| Page(s): |
1914-8 |
| Year: |
1996 |
| Keyword(s): |
GENBANK/X83935
GENBANK/X83936
GENBANK/X83937
GENBANK/X83938
GENBANK/X83939
GENBANK/X83940
GENBANK/X83941
GENBANK/X83942
GENBANK/X83943
GENBANK/X83944
GENBANK/X83945
GENBANK/X83946
GENBANK/X83947
GENBANK/X83948
GENBANK/X83949
GENBANK/X83950
GENBANK/X83951
GENBANK/X83952
GENBANK/X83953
GENBANK/X83954
Bacteroides fragilis/*classification/*genetics
Base Sequence
Cytophagaceae/classification/genetics
*DNA Transposable Elements
DNA, Bacterial
DNA, Ribosomal
Flavobacterium/classification/genetics
*Genes, Bacterial
Molecular Sequence Data
Multigene Family
Phylogeny
Support, Non-U.S. Gov't
beta-Lactamases/classification/*genetics
|
| Remarks: |
Small-subunit ribosomal DNA sequences of 16 strains of Bacteroides fragilis were determined and compared with previously published sequences. Three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods) as well as a bootstrap analysis were used to assess the robustness of each topology. All phylogenetic analyses demonstrated that the B. fragilis strains were clearly divided into two robust monophyletic units which corresponded to the cfiA-negative and cfiA-positive groups. Strains of two previously identified DNA homology groups separated similarly into the two monophyletic units. According to the intensity of the hybridization signal with a cfiA probe, the cfiA-positive cluster could be further divided into two groups. This difference might reflect the existence of two, probably closely related cfiA-type genes. In the strongly hybridizing cfiA-positive strains, the gene is capable of conferring high-level resistance to the carbapenems and to most beta-lactamase inhibitors as well, while in the weakly hybridizing cfiA-positive strains, only the latter type of resistance is known to occur. The presence of the cfiA-type genes within a monophyletic cluster of B. fragilis that apparently represents only a minority of the species B. fragilis is suggestive of a recent acquisition. The fact that this cluster is also the predominant pool of all known B. fragilis insertion elements, which have been found to play an important role in the expression of carbapenem resistance, raises the possibility that both genetic determinants, i.e., the resistance gene(s) and insertion elements, may have coevolved. |
| URL: |
96178954 |
|
| Ref #: |
1300 |
| Author(s): |
Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed) |
| Journal: |
Int. J. Syst. Bacteriol. |
| Title: |
Approved Lists of Bacterial Names. |
| Volume: |
30 |
| Page(s): |
225-420 |
| Year: |
1980 |
|
| Ref #: |
1394 |
| Author(s): |
Cato,E.P.;Johnson,J.L. |
| Journal: |
Int. J. Syst. Bacteriol. |
| Title: |
Reinstatement of species rank for Bacteroides fragilis, B. ovatus, B. distasonis, B. thetajotaomicron, and B. vulgatus: designation of neotype strains for Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers and Bacteroides thetajotaomicron ( |
| Volume: |
26 |
| Page(s): |
230-237 |
| Year: |
1976 |
|
| Ref #: |
1414 |
| Author(s): |
Sutter,V.L.;Barry,A.L.;Wilkins,T.D.;Zabransky,R.J. |
| Journal: |
Antimicrob. Agents Chemother. |
| Title: |
Collaborative evaluation of a proposed reference dilution method of susceptibility testing of anaerobic bacteria. |
| Volume: |
16 |
| Page(s): |
495-502 |
| Year: |
1979 |
|
| Ref #: |
1415 |
| Title: |
National Committee for Clinical Laboratory Standards. 1979. Proposed reference dilution procedure for antimicrobic susceptibility testing of anaerobic bacteria. Provisional Standard, PSM-11. |
| Year: |
1979 |
|
| Ref #: |
3968 |
| Author(s): |
Johnson,J.L. |
| Journal: |
Int. J. Syst. Bacteriol. |
| Title: |
Taxonomy of the bacteroides. I. Deoxyribonucleic acid homologies among Bacteroides fragilis and other saccharolytic Bacteroides species. |
| Volume: |
28 |
| Page(s): |
245-256 |
| Year: |
1978 |
|
| Ref #: |
6924 |
| Author(s): |
DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed) |
| Title: |
DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme. |
| Year: |
1997 |
|
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