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Bacteria Collection: Corynebacterium ulcerans

NCTC Number: NCTC 7910
Current Name: Corynebacterium ulcerans
Original Strain Reference: 20184
Other Collection No: ATCC 51799; CCUG 2708; DSM 46325; IMET 11148; PCM 554
Previous Catalogue Name: Corynebacterium ulcerans
Type Strain: Yes
Family: Corynebacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, patient with suspected diphtheria
16S rRNA Gene Sequence: >gb|X84256|NCTC 7910|C.ulcerans 16S rRNA gene.| cctggctcaggacga... >gb|X81911|CCUG 2708|C.ulcerans 16S rRNA gene (CCUG 2708).| acgaacgctggcggc... >gb|X84256|NCTC 7910|Corynebacterium ulcerans 16S rRNA gene, strain NCTC 7910.| cctggctcaggacga...
Bibliography: JEBB W H H 1948 J PATH BACT 60 403
Extended Bibliography: showhide Show bibliography
Ref #: 40078
Author(s): Riegel,P.;Ruimy,R.;de Briel,D.;Prevost,G.;Jehl,F.;Christen,R.;Monteil,H.
Journal: FEMS Microbiol Lett
Title: Taxonomy of Corynebacterium diphtheriae and related taxa, with recognition of Corynebacterium ulcerans sp. nov. nom. rev
Volume: 126
Page(s): 271-6
Year: 1995
Keyword(s): GENBANK/X81911 GENBANK/X81916 Base Composition Carbohydrate Metabolism Corynebacterium/*classification/genetics/metabolism Corynebacterium diphtheriae/*classification/genetics/metabolism Corynebacterium pseudotuberculosis/classification/genetics/metabolism DNA, Bacterial/analysis Energy Metabolism Molecular Sequence Data Nitrates/metabolism Phylogeny
Remarks: Levels of genomic DNA relatedness were determined using a S1 nuclease procedure for reference bacteria identified as biotypes of Corynebacterium diphtheriae, biovars of Corynebacterium pseudotuberculosis, and 'Corynebacterium ulcerans'. These results showed that the three species are separate taxa at the genomospecies level whereas biotypes and biovars are closely related genomically within each species. Phylogenetic analyses of small-subunit rDNA sequences revealed that 'Corynebacterium ulcerans' forms a tight cluster with Corynebacterium pseudotuberculosis within the robust branch that groups all Corynebacterium sequenced to date. Therefore, we propose that the species incertae sedis 'C. ulcerans' should be conclusively recognized as a distinct species within the genus Corynebacterium with strain CCUG 2708 = NCTC 7910 as type strain. This species is characterized by urease production and fermentation of glycogen.
URL: 7729671
Ref #: 38920
Author(s): Pascual,C.;Lawson,P.A.;Farrow,J.A.;Gimenez,M.N.;Collins,M.D.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences
Volume: 45
Page(s): 724-8
Year: 1995
Keyword(s): GENBANK/X81871 GENBANK/X81872 GENBANK/X81874 GENBANK/X84240 GENBANK/X84241 GENBANK/X84244 GENBANK/X84245 GENBANK/X84246 GENBANK/X84247 GENBANK/X84248 GENBANK/X84249 GENBANK/X84250 GENBANK/X84251 GENBANK/X84252 GENBANK/X84253 GENBANK/X84255 GENBANK/X84256 GENBANK/X84257 GENBANK/X84258 GENBANK/X84437 GENBANK/X84438 GENBANK/X84439 GENBANK/X84440 GENBANK/X84441 GENBANK/X84442 GENBANK/X84443 GENBANK/X84444 GENBANK/X84446 GENBANK/X84678 GENBANK/X84679 Base Sequence Corynebacterium/*classification/genetics DNA, Ribosomal/*chemistry Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/*genetics
Remarks: The 16S rRNA gene sequences of 30 strains representing 23 validated Corynebacterium species and 7 currently non-valid Corynebacterium species were determined. These sequences were aligned with the sequences of other Corynebacterium species and related actinomycete taxa. A comparative sequence analysis revealed that there is considerable phylogenetic depth and internal structure in the genus Corynebacterium. Turicella otitidis and the amycolate species Corynebacterium amycolatum were located at the periphery of the genus Corynebacterium. It was evident that the species of the genus Corynebacterium form a monophyletic association and, together with other chemotype IV and mycolic acid-containing taxa (including the genera Dietzia, Gordona, Mycobacterium, Nocardia, Rhodococcus, and Tsukamurella), form a natural suprageneric group.
URL: 7547291
Ref #: 95448
Author(s): Irminger-Finger,I.;Hurt,E.;Roebuck,A.;Collart,M.A.;Edelstein,S.J.
Journal: J Cell Biol
Title: MHP1, an essential gene in Saccharomyces cerevisiae required for microtubule function
Volume: 135
Page(s): 1323-39
Year: 1997
Keyword(s): GENBANK/X84256 GENBANK/X84652 Amino Acid Sequence Base Sequence Blotting, Western Cell Division Cloning, Molecular Epitope Mapping Fungal Proteins/analysis/chemistry/*genetics/metabolism Gene Deletion Gene Expression *Genes, Fungal Genetic Complementation Test Immune Sera Interphase Isoelectric Point Microtubule-Associated Proteins/analysis/chemistry/*genetics/metabolism Microtubules/metabolism/*physiology/ultrastructure Mitosis Molecular Sequence Data Phenotype Phosphorylation Saccharomyces cerevisiae/chemistry/*genetics/growth & development Saccharomyces cerevisiae Proteins Sequence Analysis
Remarks: The gene for a microtubule-associated protein (MAP), termed MHP1 (MAP-Homologous Protein 1), was isolated from Saccharomyces cerevisiae by expression cloning using antibodies specific for the Drosophila 205K MAP. MHP1 encodes an essential protein of 1,398 amino acids that contains near its COOH-terminal end a sequence homologous to the microtubule-binding domain of MAP2, MAP4, and tau. While total disruptions are lethal, NH2-terminal deletion mutations of MHP1 are viable, and the expression of the COOH-terminal two-thirds of the protein is sufficient for vegetative growth. Nonviable deletion-disruption mutations of MHP1 can be partially complemented by the expression of the Drosophila 205K MAP. Mhp1p binds to microtubules in vitro, and it is the COOH-terminal region containing the tau-homologous motif that mediates microtubule binding. Antibodies directed against a COOH-terminal peptide of Mhp1p decorate cytoplasmic microtubules and mitotic spindles as revealed by immunofluorescence microscopy. The overexpression of an NH2-terminal deletion mutation of MHP1 results in an accumulation of large-budded cells with short spindles and disturbed nuclear migration. In asynchronously growing cells that overexpress MHP1 from a multicopy plasmid, the length and number of cytoplasmic microtubules is increased and the proportion of mitotic cells is decreased, while haploid cells in which the expression of MHP1 has been silenced exhibit few microtubules. These results suggest that MHP1 is essential for the formation and/or stabilization of microtubules.
URL: 8947554
Ref #: 12513
Author(s): Riegel,P.;Ruimy,R.;de Briel,D.;Prevost,G.;Jehl,F.;Christen,R.;Monteil,H.
Journal: FEMS Microbiol Lett
Title: Taxonomy of Corynebacterium diphtheriae and related taxa, with recognition of Corynebacterium ulcerans sp. nov. nom. rev
Volume: 126
Page(s): 271-6
Year: 1995
Keyword(s): GENBANK/X81911 GENBANK/X81916 Base Composition Carbohydrates/metabolism Comparative Study Corynebacterium/*classification/genetics/metabolism Corynebacterium diphtheriae/*classification/genetics/metabolism Corynebacterium pseudotuberculosis/classification/genetics/metabolism DNA, Bacterial/analysis Energy Metabolism Molecular Sequence Data Nitrates/metabolism Phylogeny
Remarks: Levels of genomic DNA relatedness were determined using a S1 nuclease procedure for reference bacteria identified as biotypes of Corynebacterium diphtheriae, biovars of Corynebacterium pseudotuberculosis, and 'Corynebacterium ulcerans'. These results showed that the three species are separate taxa at the genomospecies level whereas biotypes and biovars are closely related genomically within each species. Phylogenetic analyses of small-subunit rDNA sequences revealed that 'Corynebacterium ulcerans' forms a tight cluster with Corynebacterium pseudotuberculosis within the robust branch that groups all Corynebacterium sequenced to date. Therefore, we propose that the species incertae sedis 'C. ulcerans' should be conclusively recognized as a distinct species within the genus Corynebacterium with strain CCUG 2708 = NCTC 7910 as type strain. This species is characterized by urease production and fermentation of glycogen.
URL: 95247014
Ref #: 12500
Author(s): Pascual,C.;Lawson,P.A.;Farrow,J.A.;Gimenez,M.N.;Collins,M.D.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences
Volume: 45
Page(s): 724-8
Year: 1995
Keyword(s): GENBANK/X81871 GENBANK/X81872 GENBANK/X81874 GENBANK/X84240 GENBANK/X84241 GENBANK/X84244 GENBANK/X84245 GENBANK/X84246 GENBANK/X84247 GENBANK/X84248 GENBANK/X84249 GENBANK/X84250 GENBANK/X84251 GENBANK/X84252 GENBANK/X84253 GENBANK/X84255 GENBANK/X84256 GENBANK/X84257 GENBANK/X84258 GENBANK/X84437 GENBANK/X84438 GENBANK/X84439 GENBANK/X84440 GENBANK/X84441 GENBANK/X84442 GENBANK/X84443 GENBANK/X84444 GENBANK/X84446 GENBANK/X84678 GENBANK/X84679 Base Sequence Corynebacterium/*classification/genetics DNA, Ribosomal/*chemistry Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/*genetics Support, Non-U.S. Gov't
Remarks: The 16S rRNA gene sequences of 30 strains representing 23 validated Corynebacterium species and 7 currently non-valid Corynebacterium species were determined. These sequences were aligned with the sequences of other Corynebacterium species and related actinomycete taxa. A comparative sequence analysis revealed that there is considerable phylogenetic depth and internal structure in the genus Corynebacterium. Turicella otitidis and the amycolate species Corynebacterium amycolatum were located at the periphery of the genus Corynebacterium. It was evident that the species of the genus Corynebacterium form a monophyletic association and, together with other chemotype IV and mycolic acid-containing taxa (including the genera Dietzia, Gordona, Mycobacterium, Nocardia, Rhodococcus, and Tsukamurella), form a natural suprageneric group.
URL: 96016725
Ref #: 12537
Author(s): Irminger-Finger,I.;Hurt,E.;Roebuck,A.;Collart,M.A.;Edelstein,S.J.
Journal: J Cell Biol
Title: MHP1, an essential gene in Saccharomyces cerevisiae required for microtubule function
Volume: 135
Page(s): 1323-39
Year: 1997
Keyword(s): GENBANK/X84256 GENBANK/X84652 Amino Acid Sequence Base Sequence Blotting, Western Cell Division Cloning, Molecular Epitope Mapping Fungal Proteins/analysis/chemistry/*genetics/metabolism Gene Deletion Gene Expression *Genes, Fungal Genetic Complementation Test Immune Sera Interphase Isoelectric Point Microtubule-Associated Proteins/analysis/chemistry/*genetics/metabolism Microtubules/metabolism/*physiology/ultrastructure Mitosis Molecular Sequence Data Phenotype Phosphorylation Saccharomyces cerevisiae/chemistry/*genetics/growth & development Sequence Analysis Support, Non-U.S. Gov't
Remarks: The gene for a microtubule-associated protein (MAP), termed MHP1 (MAP-Homologous Protein 1), was isolated from Saccharomyces cerevisiae by expression cloning using antibodies specific for the Drosophila 205K MAP. MHP1 encodes an essential protein of 1,398 amino acids that contains near its COOH-terminal end a sequence homologous to the microtubule-binding domain of MAP2, MAP4, and tau. While total disruptions are lethal, NH2-terminal deletion mutations of MHP1 are viable, and the expression of the COOH-terminal two-thirds of the protein is sufficient for vegetative growth. Nonviable deletion-disruption mutations of MHP1 can be partially complemented by the expression of the Drosophila 205K MAP. Mhp1p binds to microtubules in vitro, and it is the COOH-terminal region containing the tau-homologous motif that mediates microtubule binding. Antibodies directed against a COOH-terminal peptide of Mhp1p decorate cytoplasmic microtubules and mitotic spindles as revealed by immunofluorescence microscopy. The overexpression of an NH2-terminal deletion mutation of MHP1 results in an accumulation of large-budded cells with short spindles and disturbed nuclear migration. In asynchronously growing cells that overexpress MHP1 from a multicopy plasmid, the length and number of cytoplasmic microtubules is increased and the proportion of mitotic cells is decreased, while haploid cells in which the expression of MHP1 has been silenced exhibit few microtubules. These results suggest that MHP1 is essential for the formation and/or stabilization of microtubules.
URL: 97103182
Ref #: 4727
Author(s): Gilbert,R.;Stewart,F.C.
Journal: J. Lab. Clin. Med.
Title: Corynebacterium ulcerans; a pathogenic microorganism resembling C. diphtheriae.
Volume: 12
Page(s): 756-761
Year: 1927
Ref #: 6031
Journal: Int. J. Syst. Bacteriol.
Title: Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 54.
Volume: 45
Page(s): 619-620
Year: 1995
Data: W. H. H. Jebb, PHLS Oxford in 1949 / Human throat in 1948 / Jebb, W. H. H. (1948) J. path. Bact. 60, 403
Toxin Status: Non-toxigenic
Accession Date: 01/01/1949
History: JEBB W H H, PHLS, WALTON STREET, OXFORD
Authority: Riegel et al. 1995
Depositor: JEBB W H H
Taxonomy: TaxLink: S5070 (Corynebacterium ulcerans Riegel et al. 1995) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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