16S rRNA Gene Sequence: |
>gb|AF388390|ATCC 17802|Vibrio parahaemolyticus 16S ribosomal RNA gene, partial sequence.| ttgaacgctggcggc... >gb|AF388389|ATCC 17802|Vibrio parahaemolyticus clone Vp 27 16S ribosomal RNA gene, partialsequence.| agagtttgatcctgg... >gb|AF388388|ATCC 17802|Vibrio parahaemolyticus clone F44 16S ribosomal RNA gene, partialsequence.| agagtttgatcctgg... >gb|AF388387|ATCC 17802|Vibrio parahaemolyticus clone Vp16 16S ribosomal RNA gene, partialsequence.| agagtttgatcctgg... >gb|AF388386|ATCC 17802|Vibrio parahaemolyticus clone Vp23 16S ribosomal RNA gene, partialsequence.| agagtttgatcctgg... >gb|X56580 S83383 S83386 S83391|ATCC 17802|V.parahaemolyticus 16S ribosomal RNA.| naaattgaagagttt... >gb|X74720|ATCC 17802T|V.parahaemolyticus (ATCC 17802T) gene for 16S ribosomal RNA.| attgaagagtttgat... >gb|AY298808|ATCC 17802|Vibrio parahaemolyticus clone c52 16S-23S ribosomal RNA intergenicspacer region, complete sequence.| atacgatgattattc... >gb|AY298807|ATCC 17802|Vibrio parahaemolyticus clone c56 16S-23S ribosomal RNA intergenicspacer region, complete sequence.| atacgatgattattc... >gb|AY298806|ATCC 17802|Vibrio parahaemolyticus clone c54 16S-23S ribosomal RNA intergenicspacer region and tRNA-Glu gene, complete sequence.| aaacgatgattactc... >gb|AY298805|ATCC 17802|Vibrio parahaemolyticus clone c59 16S-23S ribosomal RNA intergenicspacer region, tRNA-Ile and tRNA-Ala genes, complete sequence.| aaacgatgattactc... >gb|AY298804|ATCC 17802|Vibrio parahaemolyticus clone c53 16S-23S ribosomal RNA intergenicspacer region, tRNA-Ile and tRNA-Ala genes, complete sequence.| aaacgatgattactc... >gb|AY298803|ATCC 17802|Vibrio parahaemolyticus clone c85 16S-23S ribosomal RNA intergenicspacer region, tRNA-Ala and tRNA-Glu genes, complete sequence.| aaacgatgattactc... >gb|AY298802|ATCC 17802|Vibrio parahaemolyticus clone c75 16S-23S ribosomal RNA intergenicspacer region, tRNA-Ile and tRNA-Ala genes, complete sequence.| aaacgatgattactc... >gb|AY298801|ATCC 17802|Vibrio parahaemolyticus clone c27 16S-23S ribosomal RNA intergenicspacer region, tRNA-Glu, tRNA-Lys, and tRNA-Val genes, completesequence.| aaacgatgattattc... >gb|AY298800|ATCC 17802|Vibrio parahaemolyticus clone c45 16S-23S ribosomal RNA intergenicspacer region, tRNA-Glu, tRNA-Lys, and tRNA-Val genes, completesequence.| aaacgatgattattc... >gb|AY298799|ATCC 17802|Vibrio parahaemolyticus clone c20 16S-23S ribosomal RNA intergenicspacer region, tRNA-Glu, tRNA-Lys, tRNA-Ala, and tRNA-Val genes,complete sequence.| agacgatgattactc... >gb|AY298798|ATCC 17802|Vibrio parahaemolyticus clone c75 16S ribosomal RNA (rrs) gene,partial sequence.| tggggaatattgcac... >gb|AY298793|ATCC 17802|Vibrio parahaemolyticus clone c20 16S ribosomal RNA (rrs) gene,partial sequence.| tggggaatattgcac... >gb|X56580 AH000275 S83383 S83386 S83391|ATCC 17802|V.parahaemolyticus 16S ribosomal RNA.| naaattgaagagttt... >gb|AY298797|ATCC 17802|Vibrio parahaemolyticus clone c59 16S ribosomal RNA (rrs) gene,partial sequence.| tggggaatattgcac... >gb|AY298796|ATCC 17802|Vibrio parahaemolyticus clone c56 16S ribosomal RNA (rrs) gene,partial sequence.| tggggaatattgcac... >gb|AY298795|ATCC 17802|Vibrio parahaemolyticus clone c85 16S ribosomal RNA (rrs) gene,partial sequence.| tggggaatattgcac... >gb|AY298794|ATCC 17802|Vibrio parahaemolyticus clone c27 16S ribosomal RNA (rrs) gene,partial sequence.| tggggaatattgcac... >gb|AY298792|ATCC 17802|Vibrio parahaemolyticus clone c45 16S ribosomal RNA (rrs) gene,partial sequence.| tggggaatattgcac... >gb|AY298791|ATCC 17802|Vibrio parahaemolyticus clone c53 16S ribosomal RNA (rrs) gene,partial sequence.| tggggaatattgcac... >gb|AY298790|ATCC 17802|Vibrio parahaemolyticus clone c54 16S ribosomal RNA (rrs) gene,partial sequence.| tggggaatattgcac... >gb|AY298789|ATCC 17802|Vibrio parahaemolyticus clone c52 16S ribosomal RNA (rrs) gene,partial sequence.| tggggaatattgcac... >gb|D11265|ATCC17802|Vibrio parahaemolyticus 16S rRNA.| atgcgtagagatctg... >gb|D11314|ATCC17802|Vibrio parahaemolyticus 16S rRNA.| gacgtcaagtcatca... >gb|AF429304|ATCC 17802|Vibrio parahaemolyticus ATCC 17802 16S-23S intergenic spacer,complete sequence.| ccttatacgatgatt...
|
Extended Bibliography: |
Show bibliography
Ref #: |
93196 |
Author(s): |
Ruimy,R.;Breittmayer,V.;Elbaze,P.;Lafay,B.;Boussemart,O.;Gauthier,M.;Christen,R. |
Journal: |
Int J Syst Bacteriol |
Title: |
Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences |
Volume: |
44 |
Page(s): |
416-26 |
Year: |
1994 |
Keyword(s): |
GENBANK/X74674
GENBANK/X74675
GENBANK/X74676
GENBANK/X74677
GENBANK/X74678
GENBANK/X74679
GENBANK/X74680
GENBANK/X74681
GENBANK/X74682
GENBANK/X74683
GENBANK/X74684
GENBANK/X74685
GENBANK/X74686
GENBANK/X74687
GENBANK/X74688
GENBANK/X74689
GENBANK/X74690
GENBANK/X74691
GENBANK/X74692
GENBANK/X74693
GENBANK/X74694
GENBANK/X74695
GENBANK/X74696
GENBANK/X74697
GENBANK/X74698
GENBANK/X74699
GENBANK/X74700
GENBANK/X74701
GENBANK/X74702
GENBANK/X74703
Aeromonas/classification/genetics
Photobacterium/classification/genetics
*Phylogeny
Plesiomonas/classification/genetics
RNA, Bacterial/*genetics
RNA, Ribosomal/*genetics
Sequence Homology, Nucleic Acid
Species Specificity
Vibrio/classification/genetics
Vibrionaceae/*classification/*genetics
|
Remarks: |
We sequenced nearly complete small-subunit rRNAs of 54 reference strains belonging to the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas. We then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the Proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony, as well as bootstrap, to assess the robustness of each topology. Our results confirmed that the family Vibrionaceae should include only Photobacterium and Vibrio species (but not Vibrio marinus); that Aeromonas species deserve family rank; and that Plesiomonas shigelloides is linked to the family Enterobacteriaceae. The genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas, together with the family Enterobacteriaceae, the family Pasteurellaceae, and probably the genus Alteromonas, form a robust monophyletic unit within the gamma 3 subgroup of the Proteobacteria. |
URL: |
7520733 |
|
Ref #: |
95519 |
Author(s): |
Venkateswaran,K.;Dohmoto,N.;Harayama,S. |
Journal: |
Appl Environ Microbiol |
Title: |
Cloning and nucleotide sequence of the gyrB gene of Vibrio parahaemolyticus and its application in detection of this pathogen in shrimp |
Volume: |
64 |
Page(s): |
681-7 |
Year: |
1998 |
Keyword(s): |
GENBANK/AF007287
GENBANK/AF007288
GENBANK/AF007289
GENBANK/AF007290
GENBANK/AF007291
GENBANK/AF007292
GENBANK/AF007293
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA Gyrase
DNA Topoisomerases, Type II/*genetics
Decapoda (Crustacea)/*microbiology
Molecular Sequence Data
Polymerase Chain Reaction
Sensitivity and Specificity
Vibrio parahaemolyticus/*genetics/*isolation & purification
|
Remarks: |
Because biochemical testing and 16S rRNA sequence analysis have proven inadequate for the differentiation of Vibrio parahaemolyticus from closely related species, we employed the gyrase B gene (gyrB) as a molecular diagnostic probe. The gyrB genes of V. parahaemolyticus and closely related Vibrio alginolyticus were cloned and sequenced. Oligonucleotide PCR primers were designed for the amplification of a 285-bp fragment from within gyrB specific for V. parahaemolyticus. These primers recognized 117 of 117 reference and wild-type V. parahaemolyticus strains, whereas amplification did not occur when 90 strains of 37 other Vibrio species or 60 strains representing 34 different nonvibrio species were tested. In 100-microliter PCR mixtures, the lower detection limits were 5 CFU for live cells and 4 pg for purified DNA. The possible application of gyrB primers for the routine identification of V. parahaemolyticus in food was examined. We developed and tested a procedure for the specific detection of the target organism in shrimp consisting of an 18-h preenrichment followed by PCR amplification of the 285-bp V. parahaemolyticus-specific fragment. This method enabled us to detect an initial inoculum of 1.5 CFU of V. parahaemolyticus cells per g of shrimp homogenate. By this approach, we were able to detect V. parahaemolyticus in all of 27 shrimp samples artificially inoculated with this bacterium. We present here a rapid, reliable, and sensitive protocol for the detection of V. parahaemolyticus in shrimp. |
URL: |
9464408 |
|
Ref #: |
95494 |
Author(s): |
Moreno,C.;Romero,J.;Espejo,R.T. |
Journal: |
Microbiology |
Title: |
Polymorphism in repeated 16S rRNA genes is a common property of type strains and environmental isolates of the genus Vibrio |
Volume: |
148 |
Page(s): |
1233-9 |
Year: |
2002 |
Keyword(s): |
GENBANK/AF388386
GENBANK/AF388387
GENBANK/AF388388
GENBANK/AF388389
GENBANK/AF388390
GENBANK/AF388391
GENBANK/AF388392
GENBANK/AF388393
GENBANK/AF388394
DNA, Bacterial/genetics
DNA, Ribosomal/genetics
Environment
Molecular Sequence Data
Nucleic Acid Heteroduplexes
Operon
Polymerase Chain Reaction
*Polymorphism, Genetic
RNA, Ribosomal, 16S/chemistry/*genetics
Repetitive Sequences, Nucleic Acid
Species Specificity
Vibrio/classification/*genetics/isolation & purification
Vibrio parahaemolyticus/genetics
|
Remarks: |
Analysis of the 16S rDNAs obtained from cultures of single colonies of either type collection strains or environmental strains of the genus Vibrio revealed the presence of polymorphism in every one of the strains examined. Polymorphism was detected by visualization of heteroduplexes produced after 16S rDNA PCR amplification, a procedure that allows for the screening of a large number of isolates. Amplified 16S rDNAs obtained from both Vibrio parahaemolyticus and an environmental strain were cloned. Their nucleotide sequences revealed differences of up to 2% among 16S rDNAs from the same strain. Polymorphic sites were concentrated in a recognized variable stem-loop of bacterial 16S rRNA that contained in some cases up to 83% of the total mismatches observed. Most of the substitutions present in the stem-loop region showed compensating base covariation. The accumulation of so many compensating changes in the stem-loop region implies that the divergence of the different versions of this stem-loop is relatively ancient. This divergence could be the result of either a selection process or a lateral transfer of independently evolved genes. |
URL: |
11932467 |
|
Ref #: |
72321 |
Author(s): |
Kita-Tsukamoto,K.;Oyaizu,H.;Nanba,K.;Simidu,U. |
Journal: |
Int J Syst Bacteriol |
Title: |
Phylogenetic relationships of marine bacteria, mainly members of the family Vibrionaceae, determined on the basis of 16S rRNA sequences |
Volume: |
43 |
Page(s): |
8-19 |
Year: |
1993 |
Keyword(s): |
GENBANK/D11169
GENBANK/D11170
GENBANK/D11171
GENBANK/D11172
GENBANK/D11173
GENBANK/D11174
GENBANK/D11175
GENBANK/D11176
GENBANK/D11177
GENBANK/D11178
GENBANK/D11179
GENBANK/D11180
GENBANK/D11181
GENBANK/D11182
GENBANK/D11183
GENBANK/D11184
GENBANK/D11185
GENBANK/D11186
GENBANK/D11187
GENBANK/D11188
GENBANK/D11189
GENBANK/D11190
GENBANK/D11191
GENBANK/D11192
GENBANK/D11193
GENBANK/D11194
GENBANK/D11195
GENBANK/D11196
GENBANK/D11197
GENBANK/D11198
etc.
Base Sequence
Molecular Sequence Data
*Phylogeny
RNA, Ribosomal, 16S/classification/*genetics/*isolation & purification
Seawater
Sequence Homology, Nucleic Acid
Vibrionaceae/*classification/*genetics/metabolism
*Water Microbiology
|
Remarks: |
The phylogenetic relationships of 50 reference strains, mostly marine bacteria which require Na+ for growth, were determined on the basis of 600 16S rRNA nucleotides by using reverse transcriptase sequencing. Strains belonging to 10 genera were included (four genera of the family Vibrionaceae, the genus Aeromonas of the family Aeromonadaceae, and the genera Alteromonas, Marinomonas, Shewanella, Pseudomonas, and Deleya). The sequences were aligned, the similarity values and evolutionary distance values were determined, and a phylogenetic tree was constructed by using the neighbor-joining method. On the basis of our results, the family Vibrionaceae was separated into at least seven groups (genera and families). Vibrio marinus clearly was on a line of descent that was remote from other vibrios. As determined by the similarity and evolutionary distance values, V. marinus is more distantly related to the family Vibrionaceae than the members of the Aeromonadaceae are. Also, Vibrio cholerae strains formed a separate group with Vibrio mimicus at the genus level. Of 30 species of the Vibrionaceae, 17 formed a large phylogenetic cluster. The genus Listonella was found to be a heterogeneous group, and the species were distributed in various subgroups of the Vibrionaceae. The separation of the family Aeromonadaceae from the family Vibrionaceae and the separation of the genera Marinomonas and Shewanella from the genus Alteromonas were confirmed in this phylogenetic study. However, a marine Pseudomonas species, Pseudomonas nautica, was clearly separated from two terrestrial Pseudomonas species. Each group that was separated by the phylogenetic analysis had characteristic 16S rRNA sequence patterns that were common only to species in that group. Therefore, the characteristic sequences described in this paper may be useful for identification purposes. |
URL: |
8427811 |
|
Ref #: |
27372 |
Author(s): |
Dorsch,M.;Lane,D.;Stackebrandt,E. |
Journal: |
Int J Syst Bacteriol |
Title: |
Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences |
Volume: |
42 |
Page(s): |
58-63 |
Year: |
1992 |
Keyword(s): |
GENBANK/L05178
GENBANK/X54744
GENBANK/X54745
GENBANK/X56575
GENBANK/X56576
GENBANK/X56577
GENBANK/X56578
GENBANK/X56579
GENBANK/X56580
GENBANK/X56581
GENBANK/X56582
GENBANK/X56583
Base Sequence
Molecular Sequence Data
Phylogeny
RNA Probes
RNA, Bacterial/*chemistry
RNA, Ribosomal, 16S/*chemistry
Vibrio/*classification/genetics/isolation & purification
|
Remarks: |
The inter- and intrageneric relationships of the genus Vibrio were investigated by performing a comparative analysis of the 16S rRNAs of 10 species, including four pathogenic representatives. The results of immunological and 5S rRNA studies were confirmed in that the genus is a neighboring taxon of the family Enterobacteriaceae. With regard to the intrageneric structure, Vibrio alginolyticus, Vibrio campbellii, Vibrio natriegens, Vibrio harveyi, Vibrio proteolyticus, Vibrio parahaemolyticus, and Vibrio vulnificus form the core of the genus, while Vibrio (Listonella) anguillarum, Vibrio diazotrophicus, and Vibrio hollisae are placed on the outskirts of the genus. Variable regions around positions 80, 180, and 450 could be used as target sites for genus- and species-specific oligonucleotide probes and polymerase chain reaction primers to be used in molecular identification. |
URL: |
1371064 |
|
Ref #: |
95454 |
Author(s): |
Gonzalez-Escalona,N.;Romero,J.;Espejo,R.T. |
Journal: |
FEMS Microbiol Lett |
Title: |
Polymorphism and gene conversion of the 16S rRNA genes in the multiple rRNA operons of Vibrio parahaemolyticus |
Volume: |
246 |
Page(s): |
213-9 |
Year: |
2005 |
Keyword(s): |
GENBANK/AY298793
GENBANK/AY298798
GENBANK/AY298799
GENBANK/AY298800
GENBANK/AY298801
GENBANK/AY298802
GENBANK/AY298803
GENBANK/AY298804
GENBANK/AY298805
GENBANK/AY298806
GENBANK/AY298807
GENBANK/AY298808
GENBANK/AY527386
GENBANK/AY527387
GENBANK/AY527388
Base Sequence
Cloning, Molecular
DNA, Ribosomal Spacer/genetics
*Gene Conversion
*Genes, rRNA
Molecular Sequence Data
*Polymorphism, Genetic
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/genetics
Sequence Analysis, DNA
Vibrio parahaemolyticus/*genetics
*rRNA Operon
|
Remarks: |
The genome sequence of a strain of Vibrio parahaemolyticus holds 11 copies of rRNA operons (rrn) with identical 16S rRNA genes (rrs). Conversely, the species type strain contains two rrs classes differing in 10 nucleotide sites within a short segment of 25 bp. Furthermore, we show here that the sequence of this particular segment largely differs between some strains of this species. We also show that of the eleven rrn operons in the species type strain, seven contain one rrs class and four the other, indicating gene conversion. Our results support the hypothesis that the rrs differences observed between strains of this species were caused by lateral transfer of an rrs segment and subsequent conversion. |
URL: |
15899408 |
|
Ref #: |
12044 |
Author(s): |
Ruimy,R.;Breittmayer,V.;Elbaze,P.;Lafay,B.;Boussemart,O.;Gauthier,M.;Christen,R. |
Journal: |
Int J Syst Bacteriol |
Title: |
Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences |
Volume: |
44 |
Page(s): |
416-26 |
Year: |
1994 |
Keyword(s): |
GENBANK/X74674
GENBANK/X74675
GENBANK/X74676
GENBANK/X74677
GENBANK/X74678
GENBANK/X74679
GENBANK/X74680
GENBANK/X74681
GENBANK/X74682
GENBANK/X74683
GENBANK/X74684
GENBANK/X74685
GENBANK/X74686
GENBANK/X74687
GENBANK/X74688
GENBANK/X74689
GENBANK/X74690
GENBANK/X74691
GENBANK/X74692
GENBANK/X74693
GENBANK/X74694
GENBANK/X74695
GENBANK/X74696
GENBANK/X74697
GENBANK/X74698
GENBANK/X74699
GENBANK/X74700
GENBANK/X74701
GENBANK/X74702
GENBANK/X74703
Aeromonas/classification/genetics
Comparative Study
Photobacterium/classification/genetics
*Phylogeny
Plesiomonas/classification/genetics
RNA, Bacterial/*genetics
RNA, Ribosomal/*genetics
Sequence Homology, Nucleic Acid
Species Specificity
Support, Non-U.S. Gov't
Vibrio/classification/genetics
Vibrionaceae/*classification/*genetics
|
Remarks: |
We sequenced nearly complete small-subunit rRNAs of 54 reference strains belonging to the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas. We then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the Proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony, as well as bootstrap, to assess the robustness of each topology. Our results confirmed that the family Vibrionaceae should include only Photobacterium and Vibrio species (but not Vibrio marinus); that Aeromonas species deserve family rank; and that Plesiomonas shigelloides is linked to the family Enterobacteriaceae. The genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas, together with the family Enterobacteriaceae, the family Pasteurellaceae, and probably the genus Alteromonas, form a robust monophyletic unit within the gamma 3 subgroup of the Proteobacteria. |
URL: |
94347604 |
|
Ref #: |
13615 |
Author(s): |
Dorsch,M.;Lane,D.;Stackebrandt,E. |
Journal: |
Int J Syst Bacteriol |
Title: |
Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences |
Volume: |
42 |
Page(s): |
58-63 |
Year: |
1992 |
Keyword(s): |
GENBANK/L05178
GENBANK/X54744
GENBANK/X54745
GENBANK/X56575
GENBANK/X56576
GENBANK/X56577
GENBANK/X56578
GENBANK/X56579
GENBANK/X56580
GENBANK/X56581
GENBANK/X56582
GENBANK/X56583
Base Sequence
Molecular Sequence Data
Phylogeny
RNA Probes
RNA, Bacterial/*chemistry
RNA, Ribosomal, 16S/*chemistry
Support, Non-U.S. Gov't
Vibrio/*classification/genetics/isolation & purification
|
Remarks: |
The inter- and intrageneric relationships of the genus Vibrio were investigated by performing a comparative analysis of the 16S rRNAs of 10 species, including four pathogenic representatives. The results of immunological and 5S rRNA studies were confirmed in that the genus is a neighboring taxon of the family Enterobacteriaceae. With regard to the intrageneric structure, Vibrio alginolyticus, Vibrio campbellii, Vibrio natriegens, Vibrio harveyi, Vibrio proteolyticus, Vibrio parahaemolyticus, and Vibrio vulnificus form the core of the genus, while Vibrio (Listonella) anguillarum, Vibrio diazotrophicus, and Vibrio hollisae are placed on the outskirts of the genus. Variable regions around positions 80, 180, and 450 could be used as target sites for genus- and species-specific oligonucleotide probes and polymerase chain reaction primers to be used in molecular identification. |
URL: |
92144377 |
|
Ref #: |
13624 |
Author(s): |
Moreno,C.;Romero,J.;Espejo,R.T. |
Journal: |
Microbiology |
Title: |
Polymorphism in repeated 16S rRNA genes is a common property of type strains and environmental isolates of the genus Vibrio |
Volume: |
148 |
Page(s): |
1233-9 |
Year: |
2002 |
Keyword(s): |
GENBANK/AF388386
GENBANK/AF388387
GENBANK/AF388388
GENBANK/AF388389
GENBANK/AF388390
GENBANK/AF388391
GENBANK/AF388392
GENBANK/AF388393
GENBANK/AF388394
Comparative Study
DNA, Bacterial/genetics
DNA, Ribosomal/genetics
Environment
Molecular Sequence Data
Nucleic Acid Heteroduplexes
Operon
Polymerase Chain Reaction
*Polymorphism (Genetics)
RNA, Ribosomal, 16S/chemistry/*genetics
Repetitive Sequences, Nucleic Acid
Species Specificity
Support, Non-U.S. Gov't
Vibrio/classification/*genetics/isolation & purification
Vibrio parahaemolyticus/genetics
|
Remarks: |
Analysis of the 16S rDNAs obtained from cultures of single colonies of either type collection strains or environmental strains of the genus Vibrio revealed the presence of polymorphism in every one of the strains examined. Polymorphism was detected by visualization of heteroduplexes produced after 16S rDNA PCR amplification, a procedure that allows for the screening of a large number of isolates. Amplified 16S rDNAs obtained from both Vibrio parahaemolyticus and an environmental strain were cloned. Their nucleotide sequences revealed differences of up to 2% among 16S rDNAs from the same strain. Polymorphic sites were concentrated in a recognized variable stem-loop of bacterial 16S rRNA that contained in some cases up to 83% of the total mismatches observed. Most of the substitutions present in the stem-loop region showed compensating base covariation. The accumulation of so many compensating changes in the stem-loop region implies that the divergence of the different versions of this stem-loop is relatively ancient. This divergence could be the result of either a selection process or a lateral transfer of independently evolved genes. |
URL: |
21930304 |
|
Ref #: |
1300 |
Author(s): |
Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed) |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Approved Lists of Bacterial Names. |
Volume: |
30 |
Page(s): |
225-420 |
Year: |
1980 |
|
Ref #: |
6015 |
Author(s): |
Fujino,T.;Sakazaki,R.;Takamura,K. |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Designation of the type strain of Vibrio parahaemolyticus and description of 200 strains of the species. |
Volume: |
24 |
Page(s): |
447-449 |
Year: |
1974 |
|
Ref #: |
6016 |
Author(s): |
Baumann,P.;Baumann,L.;Reichelt,J.I. |
Journal: |
J. Bacteriol. |
Title: |
Taxonomy of marine bacteria: Beneckea parahaemolytica and Beneckea alginolytica. |
Volume: |
113 |
Page(s): |
1144-1155 |
Year: |
1973 |
|
Ref #: |
6017 |
Author(s): |
Allen,R.;Baumann,P. |
Journal: |
J. Bacteriol. |
Title: |
Structure and arrangement of flagella in species of the genus Beneckea and Photobacterium fischeri. |
Volume: |
107 |
Page(s): |
295-302 |
Year: |
1971 |
|
|