Bacteria Collection: NCTC 10903 Vibrio parahaemolyticus

NCTC Number:

NCTC 10903

Current Name:

Vibrio parahaemolyticus

Original Strain Reference:

EB 101

Other Collection No:

ATCC 17802; CIP 75.2; DSM 10027; NCMB 1902; WDCM 00037

Previous Catalogue Name:

Vibrio parahaemolyticus

Type Strain:

Yes

Family:

Vibrionaceae

Hazard Group (ACDP):

2

Release Restrictions:

Terms & Conditions of Supply of Microbial Pathogens: Safety

Antigenic Properties:

serovar o1:k1

Conditions for growth on solid media:

Nutrient agar, 24-48 hours, 37°C, aerobic

Conditions for growth on liquid media:

nutrient broth,37, facultative anaerobe

Isolated From:

invertebrate

Whole Genome Sequence:

http://www.ebi.ac.uk/ena/data/view/ERS956171

Annotated Genome:

ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...

16S rRNA Gene Sequence:

23S rRNA Gene Sequence:

Miscellaneous Sequence Data:

>gb|AM235735|NCMB 1902|Vibrio parahaemolyticus partial gyrB gene for gyrase B protein,strain NCMB 1902.| tcgatgataactcgt...

Bibliography:

SAKAZAKI R 1953 MED J OSAKS UNIV 4 299; 1971 J BAT 107 268;BAUMANN P ET AL

Extended Bibliography:

Show bibliography

Ref #:	93196
Author(s):	Ruimy,R.;Breittmayer,V.;Elbaze,P.;Lafay,B.;Boussemart,O.;Gauthier,M.;Christen,R.
Journal:	Int J Syst Bacteriol
Title:	Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences
Volume:	44
Page(s):	416-26
Year:	1994
Keyword(s):	GENBANK/X74674 GENBANK/X74675 GENBANK/X74676 GENBANK/X74677 GENBANK/X74678 GENBANK/X74679 GENBANK/X74680 GENBANK/X74681 GENBANK/X74682 GENBANK/X74683 GENBANK/X74684 GENBANK/X74685 GENBANK/X74686 GENBANK/X74687 GENBANK/X74688 GENBANK/X74689 GENBANK/X74690 GENBANK/X74691 GENBANK/X74692 GENBANK/X74693 GENBANK/X74694 GENBANK/X74695 GENBANK/X74696 GENBANK/X74697 GENBANK/X74698 GENBANK/X74699 GENBANK/X74700 GENBANK/X74701 GENBANK/X74702 GENBANK/X74703 Aeromonas/classification/genetics Photobacterium/classification/genetics Phylogeny Plesiomonas/classification/genetics RNA, Bacterial/genetics RNA, Ribosomal/genetics Sequence Homology, Nucleic Acid Species Specificity Vibrio/classification/genetics Vibrionaceae/classification/*genetics
Remarks:	We sequenced nearly complete small-subunit rRNAs of 54 reference strains belonging to the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas. We then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the Proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony, as well as bootstrap, to assess the robustness of each topology. Our results confirmed that the family Vibrionaceae should include only Photobacterium and Vibrio species (but not Vibrio marinus); that Aeromonas species deserve family rank; and that Plesiomonas shigelloides is linked to the family Enterobacteriaceae. The genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas, together with the family Enterobacteriaceae, the family Pasteurellaceae, and probably the genus Alteromonas, form a robust monophyletic unit within the gamma 3 subgroup of the Proteobacteria.
URL:	7520733

Ref #:	95519
Author(s):	Venkateswaran,K.;Dohmoto,N.;Harayama,S.
Journal:	Appl Environ Microbiol
Title:	Cloning and nucleotide sequence of the gyrB gene of Vibrio parahaemolyticus and its application in detection of this pathogen in shrimp
Volume:	64
Page(s):	681-7
Year:	1998
Keyword(s):	GENBANK/AF007287 GENBANK/AF007288 GENBANK/AF007289 GENBANK/AF007290 GENBANK/AF007291 GENBANK/AF007292 GENBANK/AF007293 Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Gyrase DNA Topoisomerases, Type II/genetics Decapoda (Crustacea)/microbiology Molecular Sequence Data Polymerase Chain Reaction Sensitivity and Specificity Vibrio parahaemolyticus/genetics/isolation & purification
Remarks:	Because biochemical testing and 16S rRNA sequence analysis have proven inadequate for the differentiation of Vibrio parahaemolyticus from closely related species, we employed the gyrase B gene (gyrB) as a molecular diagnostic probe. The gyrB genes of V. parahaemolyticus and closely related Vibrio alginolyticus were cloned and sequenced. Oligonucleotide PCR primers were designed for the amplification of a 285-bp fragment from within gyrB specific for V. parahaemolyticus. These primers recognized 117 of 117 reference and wild-type V. parahaemolyticus strains, whereas amplification did not occur when 90 strains of 37 other Vibrio species or 60 strains representing 34 different nonvibrio species were tested. In 100-microliter PCR mixtures, the lower detection limits were 5 CFU for live cells and 4 pg for purified DNA. The possible application of gyrB primers for the routine identification of V. parahaemolyticus in food was examined. We developed and tested a procedure for the specific detection of the target organism in shrimp consisting of an 18-h preenrichment followed by PCR amplification of the 285-bp V. parahaemolyticus-specific fragment. This method enabled us to detect an initial inoculum of 1.5 CFU of V. parahaemolyticus cells per g of shrimp homogenate. By this approach, we were able to detect V. parahaemolyticus in all of 27 shrimp samples artificially inoculated with this bacterium. We present here a rapid, reliable, and sensitive protocol for the detection of V. parahaemolyticus in shrimp.
URL:	9464408

Ref #:	95494
Author(s):	Moreno,C.;Romero,J.;Espejo,R.T.
Journal:	Microbiology
Title:	Polymorphism in repeated 16S rRNA genes is a common property of type strains and environmental isolates of the genus Vibrio
Volume:	148
Page(s):	1233-9
Year:	2002
Keyword(s):	GENBANK/AF388386 GENBANK/AF388387 GENBANK/AF388388 GENBANK/AF388389 GENBANK/AF388390 GENBANK/AF388391 GENBANK/AF388392 GENBANK/AF388393 GENBANK/AF388394 DNA, Bacterial/genetics DNA, Ribosomal/genetics Environment Molecular Sequence Data Nucleic Acid Heteroduplexes Operon Polymerase Chain Reaction Polymorphism, Genetic RNA, Ribosomal, 16S/chemistry/genetics Repetitive Sequences, Nucleic Acid Species Specificity Vibrio/classification/*genetics/isolation & purification Vibrio parahaemolyticus/genetics
Remarks:	Analysis of the 16S rDNAs obtained from cultures of single colonies of either type collection strains or environmental strains of the genus Vibrio revealed the presence of polymorphism in every one of the strains examined. Polymorphism was detected by visualization of heteroduplexes produced after 16S rDNA PCR amplification, a procedure that allows for the screening of a large number of isolates. Amplified 16S rDNAs obtained from both Vibrio parahaemolyticus and an environmental strain were cloned. Their nucleotide sequences revealed differences of up to 2% among 16S rDNAs from the same strain. Polymorphic sites were concentrated in a recognized variable stem-loop of bacterial 16S rRNA that contained in some cases up to 83% of the total mismatches observed. Most of the substitutions present in the stem-loop region showed compensating base covariation. The accumulation of so many compensating changes in the stem-loop region implies that the divergence of the different versions of this stem-loop is relatively ancient. This divergence could be the result of either a selection process or a lateral transfer of independently evolved genes.
URL:	11932467

Ref #:	72321
Author(s):	Kita-Tsukamoto,K.;Oyaizu,H.;Nanba,K.;Simidu,U.
Journal:	Int J Syst Bacteriol
Title:	Phylogenetic relationships of marine bacteria, mainly members of the family Vibrionaceae, determined on the basis of 16S rRNA sequences
Volume:	43
Page(s):	8-19
Year:	1993
Keyword(s):	GENBANK/D11169 GENBANK/D11170 GENBANK/D11171 GENBANK/D11172 GENBANK/D11173 GENBANK/D11174 GENBANK/D11175 GENBANK/D11176 GENBANK/D11177 GENBANK/D11178 GENBANK/D11179 GENBANK/D11180 GENBANK/D11181 GENBANK/D11182 GENBANK/D11183 GENBANK/D11184 GENBANK/D11185 GENBANK/D11186 GENBANK/D11187 GENBANK/D11188 GENBANK/D11189 GENBANK/D11190 GENBANK/D11191 GENBANK/D11192 GENBANK/D11193 GENBANK/D11194 GENBANK/D11195 GENBANK/D11196 GENBANK/D11197 GENBANK/D11198 etc. Base Sequence Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/classification/genetics/isolation & purification Seawater Sequence Homology, Nucleic Acid Vibrionaceae/classification/genetics/metabolism Water Microbiology
Remarks:	The phylogenetic relationships of 50 reference strains, mostly marine bacteria which require Na+ for growth, were determined on the basis of 600 16S rRNA nucleotides by using reverse transcriptase sequencing. Strains belonging to 10 genera were included (four genera of the family Vibrionaceae, the genus Aeromonas of the family Aeromonadaceae, and the genera Alteromonas, Marinomonas, Shewanella, Pseudomonas, and Deleya). The sequences were aligned, the similarity values and evolutionary distance values were determined, and a phylogenetic tree was constructed by using the neighbor-joining method. On the basis of our results, the family Vibrionaceae was separated into at least seven groups (genera and families). Vibrio marinus clearly was on a line of descent that was remote from other vibrios. As determined by the similarity and evolutionary distance values, V. marinus is more distantly related to the family Vibrionaceae than the members of the Aeromonadaceae are. Also, Vibrio cholerae strains formed a separate group with Vibrio mimicus at the genus level. Of 30 species of the Vibrionaceae, 17 formed a large phylogenetic cluster. The genus Listonella was found to be a heterogeneous group, and the species were distributed in various subgroups of the Vibrionaceae. The separation of the family Aeromonadaceae from the family Vibrionaceae and the separation of the genera Marinomonas and Shewanella from the genus Alteromonas were confirmed in this phylogenetic study. However, a marine Pseudomonas species, Pseudomonas nautica, was clearly separated from two terrestrial Pseudomonas species. Each group that was separated by the phylogenetic analysis had characteristic 16S rRNA sequence patterns that were common only to species in that group. Therefore, the characteristic sequences described in this paper may be useful for identification purposes.
URL:	8427811

Ref #:	27372
Author(s):	Dorsch,M.;Lane,D.;Stackebrandt,E.
Journal:	Int J Syst Bacteriol
Title:	Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences
Volume:	42
Page(s):	58-63
Year:	1992
Keyword(s):	GENBANK/L05178 GENBANK/X54744 GENBANK/X54745 GENBANK/X56575 GENBANK/X56576 GENBANK/X56577 GENBANK/X56578 GENBANK/X56579 GENBANK/X56580 GENBANK/X56581 GENBANK/X56582 GENBANK/X56583 Base Sequence Molecular Sequence Data Phylogeny RNA Probes RNA, Bacterial/chemistry RNA, Ribosomal, 16S/chemistry Vibrio/*classification/genetics/isolation & purification
Remarks:	The inter- and intrageneric relationships of the genus Vibrio were investigated by performing a comparative analysis of the 16S rRNAs of 10 species, including four pathogenic representatives. The results of immunological and 5S rRNA studies were confirmed in that the genus is a neighboring taxon of the family Enterobacteriaceae. With regard to the intrageneric structure, Vibrio alginolyticus, Vibrio campbellii, Vibrio natriegens, Vibrio harveyi, Vibrio proteolyticus, Vibrio parahaemolyticus, and Vibrio vulnificus form the core of the genus, while Vibrio (Listonella) anguillarum, Vibrio diazotrophicus, and Vibrio hollisae are placed on the outskirts of the genus. Variable regions around positions 80, 180, and 450 could be used as target sites for genus- and species-specific oligonucleotide probes and polymerase chain reaction primers to be used in molecular identification.
URL:	1371064

Ref #:	95454
Author(s):	Gonzalez-Escalona,N.;Romero,J.;Espejo,R.T.
Journal:	FEMS Microbiol Lett
Title:	Polymorphism and gene conversion of the 16S rRNA genes in the multiple rRNA operons of Vibrio parahaemolyticus
Volume:	246
Page(s):	213-9
Year:	2005
Keyword(s):	GENBANK/AY298793 GENBANK/AY298798 GENBANK/AY298799 GENBANK/AY298800 GENBANK/AY298801 GENBANK/AY298802 GENBANK/AY298803 GENBANK/AY298804 GENBANK/AY298805 GENBANK/AY298806 GENBANK/AY298807 GENBANK/AY298808 GENBANK/AY527386 GENBANK/AY527387 GENBANK/AY527388 Base Sequence Cloning, Molecular DNA, Ribosomal Spacer/genetics Gene Conversion Genes, rRNA Molecular Sequence Data Polymorphism, Genetic RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Sequence Analysis, DNA Vibrio parahaemolyticus/genetics rRNA Operon
Remarks:	The genome sequence of a strain of Vibrio parahaemolyticus holds 11 copies of rRNA operons (rrn) with identical 16S rRNA genes (rrs). Conversely, the species type strain contains two rrs classes differing in 10 nucleotide sites within a short segment of 25 bp. Furthermore, we show here that the sequence of this particular segment largely differs between some strains of this species. We also show that of the eleven rrn operons in the species type strain, seven contain one rrs class and four the other, indicating gene conversion. Our results support the hypothesis that the rrs differences observed between strains of this species were caused by lateral transfer of an rrs segment and subsequent conversion.
URL:	15899408

Ref #:	12044
Author(s):	Ruimy,R.;Breittmayer,V.;Elbaze,P.;Lafay,B.;Boussemart,O.;Gauthier,M.;Christen,R.
Journal:	Int J Syst Bacteriol
Title:	Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences
Volume:	44
Page(s):	416-26
Year:	1994
Keyword(s):	GENBANK/X74674 GENBANK/X74675 GENBANK/X74676 GENBANK/X74677 GENBANK/X74678 GENBANK/X74679 GENBANK/X74680 GENBANK/X74681 GENBANK/X74682 GENBANK/X74683 GENBANK/X74684 GENBANK/X74685 GENBANK/X74686 GENBANK/X74687 GENBANK/X74688 GENBANK/X74689 GENBANK/X74690 GENBANK/X74691 GENBANK/X74692 GENBANK/X74693 GENBANK/X74694 GENBANK/X74695 GENBANK/X74696 GENBANK/X74697 GENBANK/X74698 GENBANK/X74699 GENBANK/X74700 GENBANK/X74701 GENBANK/X74702 GENBANK/X74703 Aeromonas/classification/genetics Comparative Study Photobacterium/classification/genetics Phylogeny Plesiomonas/classification/genetics RNA, Bacterial/genetics RNA, Ribosomal/genetics Sequence Homology, Nucleic Acid Species Specificity Support, Non-U.S. Gov't Vibrio/classification/genetics Vibrionaceae/classification/*genetics
Remarks:	We sequenced nearly complete small-subunit rRNAs of 54 reference strains belonging to the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas. We then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the Proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony, as well as bootstrap, to assess the robustness of each topology. Our results confirmed that the family Vibrionaceae should include only Photobacterium and Vibrio species (but not Vibrio marinus); that Aeromonas species deserve family rank; and that Plesiomonas shigelloides is linked to the family Enterobacteriaceae. The genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas, together with the family Enterobacteriaceae, the family Pasteurellaceae, and probably the genus Alteromonas, form a robust monophyletic unit within the gamma 3 subgroup of the Proteobacteria.
URL:	94347604

Ref #:	13615
Author(s):	Dorsch,M.;Lane,D.;Stackebrandt,E.
Journal:	Int J Syst Bacteriol
Title:	Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences
Volume:	42
Page(s):	58-63
Year:	1992
Keyword(s):	GENBANK/L05178 GENBANK/X54744 GENBANK/X54745 GENBANK/X56575 GENBANK/X56576 GENBANK/X56577 GENBANK/X56578 GENBANK/X56579 GENBANK/X56580 GENBANK/X56581 GENBANK/X56582 GENBANK/X56583 Base Sequence Molecular Sequence Data Phylogeny RNA Probes RNA, Bacterial/chemistry RNA, Ribosomal, 16S/chemistry Support, Non-U.S. Gov't Vibrio/*classification/genetics/isolation & purification
Remarks:	The inter- and intrageneric relationships of the genus Vibrio were investigated by performing a comparative analysis of the 16S rRNAs of 10 species, including four pathogenic representatives. The results of immunological and 5S rRNA studies were confirmed in that the genus is a neighboring taxon of the family Enterobacteriaceae. With regard to the intrageneric structure, Vibrio alginolyticus, Vibrio campbellii, Vibrio natriegens, Vibrio harveyi, Vibrio proteolyticus, Vibrio parahaemolyticus, and Vibrio vulnificus form the core of the genus, while Vibrio (Listonella) anguillarum, Vibrio diazotrophicus, and Vibrio hollisae are placed on the outskirts of the genus. Variable regions around positions 80, 180, and 450 could be used as target sites for genus- and species-specific oligonucleotide probes and polymerase chain reaction primers to be used in molecular identification.
URL:	92144377

Ref #:	13624
Author(s):	Moreno,C.;Romero,J.;Espejo,R.T.
Journal:	Microbiology
Title:	Polymorphism in repeated 16S rRNA genes is a common property of type strains and environmental isolates of the genus Vibrio
Volume:	148
Page(s):	1233-9
Year:	2002
Keyword(s):	GENBANK/AF388386 GENBANK/AF388387 GENBANK/AF388388 GENBANK/AF388389 GENBANK/AF388390 GENBANK/AF388391 GENBANK/AF388392 GENBANK/AF388393 GENBANK/AF388394 Comparative Study DNA, Bacterial/genetics DNA, Ribosomal/genetics Environment Molecular Sequence Data Nucleic Acid Heteroduplexes Operon Polymerase Chain Reaction Polymorphism (Genetics) RNA, Ribosomal, 16S/chemistry/genetics Repetitive Sequences, Nucleic Acid Species Specificity Support, Non-U.S. Gov't Vibrio/classification/*genetics/isolation & purification Vibrio parahaemolyticus/genetics
Remarks:	Analysis of the 16S rDNAs obtained from cultures of single colonies of either type collection strains or environmental strains of the genus Vibrio revealed the presence of polymorphism in every one of the strains examined. Polymorphism was detected by visualization of heteroduplexes produced after 16S rDNA PCR amplification, a procedure that allows for the screening of a large number of isolates. Amplified 16S rDNAs obtained from both Vibrio parahaemolyticus and an environmental strain were cloned. Their nucleotide sequences revealed differences of up to 2% among 16S rDNAs from the same strain. Polymorphic sites were concentrated in a recognized variable stem-loop of bacterial 16S rRNA that contained in some cases up to 83% of the total mismatches observed. Most of the substitutions present in the stem-loop region showed compensating base covariation. The accumulation of so many compensating changes in the stem-loop region implies that the divergence of the different versions of this stem-loop is relatively ancient. This divergence could be the result of either a selection process or a lateral transfer of independently evolved genes.
URL:	21930304

Ref #:	1300
Author(s):	Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal:	Int. J. Syst. Bacteriol.
Title:	Approved Lists of Bacterial Names.
Volume:	30
Page(s):	225-420
Year:	1980

Ref #:	6015
Author(s):	Fujino,T.;Sakazaki,R.;Takamura,K.
Journal:	Int. J. Syst. Bacteriol.
Title:	Designation of the type strain of Vibrio parahaemolyticus and description of 200 strains of the species.
Volume:	24
Page(s):	447-449
Year:	1974

Ref #:	6016
Author(s):	Baumann,P.;Baumann,L.;Reichelt,J.I.
Journal:	J. Bacteriol.
Title:	Taxonomy of marine bacteria: Beneckea parahaemolytica and Beneckea alginolytica.
Volume:	113
Page(s):	1144-1155
Year:	1973

Ref #:	6017
Author(s):	Allen,R.;Baumann,P.
Journal:	J. Bacteriol.
Title:	Structure and arrangement of flagella in species of the genus Beneckea and Photobacterium fischeri.
Volume:	107
Page(s):	295-302
Year:	1971

Data:

(P. Baumann 113, ATCC 17802) Type strain / ATCC in 1973 / T. Fujino / Shirasu, food poisoning / 01:K1 / Kanagawa negative / Fujino, T. et al. (1953) Med. J. Osaks. Univ. 4, 299 / Sakazaki, R. (1968) Jap. J. Med. Sci. Biol. 21, 359 / Baumann, P. et al. (1971) J. Bact. 107, 268

Accession Date:

01/01/1973

History:

ISOLATED BY FUJINO T

Authority:

(Fujino et al. 1951) Sakazaki et al. 1963

Depositor:

ATCC

Taxonomy:

TaxLink: S3562 (Vibrio parahaemolyticus (Fujino et al. 1951) Sakazaki et al. 1963) - Date of change: 5/02/2003

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