Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Citrobacter amalonaticus

NCTC Number: NCTC 10805
Current Name: Citrobacter amalonaticus
Original Strain Reference: ATCC 25405
Other Collection No: ATCC 25405; CIP 82.89; DSM 4593
Previous Catalogue Name: Levinea amalonatica
Type Strain: Yes
Family: Enterobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human
Whole Genome Sequence:
Annotated Genome:
Miscellaneous Sequence Data: >gb|AY370832|ATCC 25405|Citrobacter amalonaticus strain ATCC 25405 gyrase B (gyrB) gene,partial cds.| aaagtctccggcggt...
Bibliography: YOUNG V M 1971 INT J SYST BACT 21 58
Extended Bibliography: showhide Show bibliography
Ref #: 43181
Author(s): Delmas,J.;Breysse,F.;Devulder,G.;Flandrois,J.P.;Chomarat,M.
Journal: Diagn Microbiol Infect Dis
Title: Rapid identification of Enterobacteriaceae by sequencing DNA gyrase subunit B encoding gene
Volume: 55
Page(s): 263-8
Year: 2006
Keyword(s): Bacterial Typing Techniques/*methods DNA Gyrase/*genetics Enterobacteriaceae/enzymology/genetics/*isolation & purification Genotype Humans Sequence Analysis, DNA/methods
Remarks: Real-time polymerase chain reaction and sequencing were used to characterize a 506-bp-long DNA fragment internal to the gyrB gene (gyrBint). The sequences obtained from 32 Enterobacteriaceae-type strains and those available in the Genbank nucleotide sequence database (n = 24) were used as a database to identify 240 clinical enterobacteria isolates. Sequence analysis of the gyrBint fragment of 240 strains showed that gyrBint constitutes a discriminative target sequence to differentiate between Enterobacteriaceae species. Comparison of these identifications with those obtained by phenotypic methods (Vitek 1 system and/or Rapid ID 32E; bioMerieux, Marcy l'Etoile, France) revealed discrepancies essentially with genera Citrobacter and Enterobacter. Most of the strains identified as Enterobacter cloacae by phenotypic methods were identified as Enterobacter hormaechei strains by gyrBint sequencing. The direct sequencing of gyrBint would be useful as a complementary tool in the identification of clinical Enterobacteriaceae isolates.
URL: 16626902
Ref #: 2315
Journal: Int. J. Syst. Bacteriol.
Title: Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 8.
Volume: 32
Page(s): 266-268
Year: 1982
Ref #: 6779
Author(s): FarmerIII,J.J.
Title: The genus Citrobacter.
Page(s): 1140-1147
Data: Type strain / V. M. Young, Baltimore in 1971 / Faeces / Young, V. M. et al. (1971) Int. J. syst. Bact. 21, 58
Accession Date: 01/01/1971
Authority: (Young et al. 1971) Brenner and Farmer 1982
Depositor: YOUNG V M
Taxonomy: TaxLink: S798 (Levinea amalonatica) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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Ampoule (Bacteria)

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