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Bacteria Collection: Stenotrophomonas maltophilia

NCTC Number: NCTC 10257
Current Name: Stenotrophomonas maltophilia
Original Strain Reference: RH 810-2
Other Collection No: ATCC 13637; DSM 50170; ICPB 2648-67; NCIB 9203; NCPPB 1974; NRC 729; RH 810-2
Previous Catalogue Name: Stenotrophomonas maltophilia
Type Strain: Yes
Family: Xanthomonadaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37,, aerobic
Isolated From: human, mouth
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1247815
16S rRNA Gene Sequence: >gb|AB008509|ATCC 13637T|Stenotrophomonas maltophilia gene for 16S rRNA, partial sequence.| agtgaacgctggcgg...
23S rRNA Gene Sequence: >gb|AF273255|ATCC13637|Stenotrophomonas maltophilia 23S ribosomal RNA gene, partialsequence.| ggtcaagcgaataag...
Miscellaneous Sequence Data: >gb|AJ409326|ATCC 13637|Stenotrophomonas maltophilia partial gyrB gene for gyrase B.| atgacccgccgtaag...
Extended Bibliography: showhide Show bibliography
Ref #: 95522
Author(s): Iizuka,T.;Yamanaka,S.;Nishiyama,T.;Hiraishi,A.
Journal: J Gen Appl Microbiol
Title: Isolation and phylogenetic analysis of aerobic copiotrophic ultramicrobacteria from urban soil
Volume: 44
Page(s): 75-84
Year: 2002
Remarks: Free-living, aerobic, copiotrophic ultramicrobacteria (UMB) that passed through a 0.45 &mgr;m membrane filter and had a cell volume of less than 0.3 &mgr;m(3) were isolated from polluted urban soil by using both the direct plating method and the membrane-filter enrichment technique. The efficiency of recovering UMB from the soil was much higher in the latter method than in the former. All of the UMB isolates grew well with a doubling time of less than 6 h either in a complex nutrient medium or a chemically defined medium. The average cell volumes of the UMB isolates, as measured by scanning electron microscopy and epifluorescent microscopy with an image analysis, ranged from 0.07 to 0.22 &mgr;m(3). The cell size was larger at the exponential phase of growth than at the stationary growth stage in general. Ultrathin-section electron microscopy of representatives of the UMB isolates showed that they had complete cell wall structures like typical Gram-negative or -positive bacteria. Phenotypic studies and phylogenetic analyses on the basis of 16S rDNA sequences showed that the UMB isolates were classified into three major groups, the beta and gamma subdivisions of the Proteobacteria and the Actinobacteria (the high G+C DNA group of Gram-positives). However, none of these isolates were assigned to any previously known species. These results demonstrate that free-living, relatively fast-growing, copiotrophic UMB strains undescribed so far are widely distributed in terrestrial environments, including urban soil.
URL: 12501296
Ref #: 95503
Author(s): Whitby,P.W.;Carter,K.B.;Burns,J.L.;Royall,J.A.;LiPuma,J.J.;Stull,T.L.
Journal: J Clin Microbiol
Title: Identification and detection of Stenotrophomonas maltophilia by rRNA-directed PCR
Volume: 38
Page(s): 4305-9
Year: 2001
Keyword(s): Cloning, Molecular Cystic Fibrosis/microbiology Humans Polymerase Chain Reaction/*methods RNA, Ribosomal, 23S/*genetics Sputum/microbiology Stenotrophomonas maltophilia/genetics/*isolation & purification
Remarks: Stenotrophomonas maltophilia has recently emerged as an important nosocomial pathogen in immunocompromised patients, in transplant recipients, and in persons with cystic fibrosis (CF). While this organism is nonpathogenic in healthy individuals, it is increasingly associated with morbidity and mortality in susceptible populations. Recent studies have indicated that for approximately 10% of CF patients with moderate lung disease, S. maltophilia can be cultured from respiratory tract secretions. Identification of S. maltophilia can be problematic, and analysis of isolates from the Burkholderia cepacia Research Laboratory and Repository showed that several isolates presumptively identified as B. cepacia by clinical microbiology laboratories were in fact S. maltophilia. To overcome the problems associated with definitive identification, we developed species-specific PCR (SS-PCR) primers, designated SM1 and SM4, directed to the 23S rRNA gene, and tested their utility to accurately identify S. maltophilia directly from sputum. The SS-PCR was developed and tested against a panel of 112 S. maltophilia isolates collected from diverse geographic locations. To test for specificity, 43 isolates from 17 different species were analyzed. PCR with the SM1-SM4 primer pair and isolated genomic DNA as a template resulted in amplification of a band from all S. maltophilia isolates and was uniformly negative for all other species tested, yielding a sensitivity and a specificity of 100% for the SS-PCR. The utility of the SS-PCR to directly identify S. maltophilia in sputum was examined. Thirteen expectorated sputum samples from CF patients were analyzed by SS-PCR. Three samples were PCR positive, in complete concordance with the conventional laboratory culture. Thus, we have developed an SS-PCR protocol that can rapidly and accurately identify S. maltophilia isolates and which can be used for the direct detection of this organism in CF patient sputum.
URL: 11101555
Ref #: 95466
Author(s): Valdezate,S.;Vindel,A.;Echeita,A.;Baquero,F.;Canto,R.
Journal: Antimicrob Agents Chemother
Title: Topoisomerase II and IV quinolone resistance-determining regions in Stenotrophomonas maltophilia clinical isolates with different levels of quinolone susceptibility
Volume: 46
Page(s): 665-71
Year: 2002
Keyword(s): 4-Quinolones Amino Acid Sequence Anti-Infective Agents/*pharmacology Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology DNA Gyrase/genetics DNA Topoisomerase IV/*genetics DNA Topoisomerases, Type II/*genetics Drug Resistance, Microbial Gram-Negative Bacterial Infections/*microbiology Microbial Sensitivity Tests Molecular Sequence Data Phenotype Reserpine/pharmacology Reverse Transcriptase Polymerase Chain Reaction Stenotrophomonas maltophilia/*drug effects Uncoupling Agents/pharmacology
Remarks: The quinolone resistance-determining regions (QRDRs) of topoisomerase II and IV genes from Stenotrophomonas maltophilia ATCC 13637 were sequenced and compared with the corresponding regions of 32 unrelated S. maltophilia clinical strains for which ciprofloxacin MICs ranged from 0.1 to 64 microg/ml. GyrA (Leu-55 to Gln-155, Escherichia coli numbering), GyrB (Met-391 to Phe-513), ParC (Ile-34 to Arg-124), and ParE (Leu-396 to Leu-567) fragments from strain ATCC 13637 showed high degrees of identity to the corresponding regions from the phytopathogen Xylella fastidiosa, with the degrees of identity ranging from 85.0 to 93.5%. Lower degrees of identity to the corresponding regions from Pseudomonas aeruginosa (70.9 to 88.6%) and E. coli (73.0 to 88.6%) were observed. Amino acid changes were present in GyrA fragments from 9 of the 32 strains at positions 70, 85, 90, 103, 112, 113, 119, and 124; but there was no consistent relation to higher ciprofloxacin MICs. The absence of changes at positions 83 and 87, commonly involved in quinolone resistance in gram-negative bacteria, was unexpected. The GyrB sequences were identical in all strains, and only one strain (ciprofloxacin MIC, 16 microg/ml) showed a ParC amino acid change (Ser-80-->Arg). In contrast, a high frequency (16 of 32 strains) of amino acid replacements was present in ParE. The frequencies of alterations at positions 437, 465, 477, and 485 were higher (P < 0.05) in strains from cystic fibrosis patients, but these changes were not linked with high ciprofloxacin MICs. An efflux phenotype, screened by the detection of decreases of at least twofold doubling dilutions of the ciprofloxacin MIC in the presence of carbonyl cyanide m-chlorophenylhydrazone (0.5 microg/ml) or reserpine (10 microg/ml), was suspected in seven strains. These results suggest that topoisomerases II and IV may not be the primary targets involved in quinolone resistance in S. maltophilia.
URL: 11850246
Ref #: 13704
Author(s): Whitby,P.W.;Carter,K.B.;Burns,J.L.;Royall,J.A.;LiPuma,J.J.;Stull,T.L.
Journal: J Clin Microbiol
Title: Identification and detection of Stenotrophomonas maltophilia by rRNA-directed PCR
Volume: 38
Page(s): 4305-9
Year: 2001
Keyword(s): Cloning, Molecular Cystic Fibrosis/microbiology Human Polymerase Chain Reaction/*methods RNA, Ribosomal, 23S/*genetics Sputum/microbiology Stenotrophomonas maltophilia/genetics/*isolation & purification Support, Non-U.S. Gov't
Remarks: Stenotrophomonas maltophilia has recently emerged as an important nosocomial pathogen in immunocompromised patients, in transplant recipients, and in persons with cystic fibrosis (CF). While this organism is nonpathogenic in healthy individuals, it is increasingly associated with morbidity and mortality in susceptible populations. Recent studies have indicated that for approximately 10% of CF patients with moderate lung disease, S. maltophilia can be cultured from respiratory tract secretions. Identification of S. maltophilia can be problematic, and analysis of isolates from the Burkholderia cepacia Research Laboratory and Repository showed that several isolates presumptively identified as B. cepacia by clinical microbiology laboratories were in fact S. maltophilia. To overcome the problems associated with definitive identification, we developed species-specific PCR (SS-PCR) primers, designated SM1 and SM4, directed to the 23S rRNA gene, and tested their utility to accurately identify S. maltophilia directly from sputum. The SS-PCR was developed and tested against a panel of 112 S. maltophilia isolates collected from diverse geographic locations. To test for specificity, 43 isolates from 17 different species were analyzed. PCR with the SM1-SM4 primer pair and isolated genomic DNA as a template resulted in amplification of a band from all S. maltophilia isolates and was uniformly negative for all other species tested, yielding a sensitivity and a specificity of 100% for the SS-PCR. The utility of the SS-PCR to directly identify S. maltophilia in sputum was examined. Thirteen expectorated sputum samples from CF patients were analyzed by SS-PCR. Three samples were PCR positive, in complete concordance with the conventional laboratory culture. Thus, we have developed an SS-PCR protocol that can rapidly and accurately identify S. maltophilia isolates and which can be used for the direct detection of this organism in CF patient sputum.
URL: 20553323
Ref #: 171
Author(s): Stanier,R.Y.;Palleroni,N.J.;DoudoroffM.
Journal: J. Gen. Microbiol.
Title: The aerobic pseudomonads: a taxonomic study.
Volume: 43
Page(s): 159-275
Year: 1966
Ref #: 1801
Author(s): Hugh,R.
Journal: Int. J. Syst. Bacteriol.
Title: Pseudomonas maltophilia sp. nov. nom. rev.
Volume: 31
Page(s): 195
Year: 1981
Ref #: 4029
Author(s): Owen,R.J.;Jackman,P.J.H.
Journal: J. Gen. Microbiol.
Title: The similarities between Pseudomonas paucimobilis and allied bacteria derived from analysis of DNA and electrophoretic protein patterns.
Volume: 128
Page(s): 2945-2954
Year: 1982
Ref #: 4851
Author(s): Palleroni,N.J.;Bradbury,J.F.
Journal: Int. J. Syst. Bacteriol.
Title: Stenotrophomonas, a new bacterial genus for Xanthomonas maltophila (Hugh 1980) Swings et al. 1983.
Volume: 43
Page(s): 606-609
Year: 1993
Ref #: 4934
Journal: Int. J. Syst. Bacteriol.
Title: Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 47.
Volume: 43
Page(s): 864-865
Year: 1993
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Data: (ATCC 13637, NCIB 9203, NCPPB 1974, DSM 50170, NRC 729) Type strain / ATCC in 1961 / Mouth / Hugh, R. & Ryschenkow, E. (1961) J. gen. Microbiol. 26, 123 / Hugh, R. & Leifson, E. (1963) Int. Bull. bact. Nomencl. Taxon. 13, 133
Accession Date: 01/01/1961
Authority: (Hugh 1981) Palleroni and Bradbury 1993
Depositor: ATCC
Taxonomy: TaxLink: S3596 (Pseudomonas maltophilia) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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