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YTS 121.5.2

YTS 121.5.2

Catalogue No.

87072283

Cell Line Name

YTS 121.5.2

Cell Line Description

Rat Y3Ag1 .2.3 x Rat DA spleen.

 

The Ab is directed against the Lyt-1 T cell Ag, the murine equivalent of CD5, a cell surface glycoprotein expressed by 95% of T-lymphocytes and 72% of peripheral blood lymphocytes. Anti-CD5.

General Info

Fusion Species

Rat x Rat Hybridoma

Unique to ECACC

Yes

Nagoya Status

Not Applicable

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Myeloma

Y3Ag1.2.3

Immunogen

C.BA mouse thymocytes

Immunological Donor

DA rat spleen

Antibody Isotype

Ig2b

Morphology

Spherical

Tissue of Origin

Hybrid

Culture Conditions

Cell Type

Hybridoma

Subculture Routine

Maintain cultures between 3-9 x100,000 cells/ml; 5% CO2; 37°C. Cells may adhere to the plastic flask - may require trypsin or dislodging by pipetting to remove from the plastic.

Culture Medium

RPMI 1640 + 2mM glutamine + 0.05mM 2-mercaptoethanol (2ME) + 20% Foetal Bovine Serum (FBS)

Growth Mode

Semi-adherent

Additional Info

Depositor

Prof H Waldmann/Dr SP Cobbold. Dept. of Pathology. University of Cambridge

Country of Origin

United Kingdom

Applications

Flow cytometry, Western Blot, ELISA

Patent

None

GMO Status

Not Applicable

Hazard Group (ACDP)

1

References

References

Cobbold SP, Jayasuriya A, Nash A, Prospero TD, Waldmann H. Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo. Nature. 1984 Dec 6-12;312(5994):548-51. doi: 10.1038/312548a0. PMID: 6150440.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: YTS 121.5.2 (ECACC 87072283)

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.