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SK-N-F1

SK-N-F1

Catalogue No.

94092304

Cell Line Name

SK-N-F1

Cell Line Description

SK-N-F1 has been obtained from a bone marrow metastasis of a male patient with neuroblastoma. The poorly differentiated and slow growing cells secrete different neuroblastoma specific peptides and synthesise choline acetyltransferase. Reactivity of anti-leukaemia-associated and anti-foetal brain-associated monoclonal antibodies could be shown. Epithelial-like and aggregate-forming populations.

Characteristics

Receptors

Transferrin receptor

Tissue of Origin

Neural (bone marrow metastasis)

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 10,12
D5S818: 12,13
D7S820: 8,9
D13S317: 11,14
D16S539: 11,12
TH01: 6,9.3
TPOX: 8,9
vWA: 16,17

Karyotype

Aneuploid

Applications

Carcinogenesis studies, cytotoxicity assays

Disease

Neuroblastoma

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) seeding at 2-4 x104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO; 37°C. Clumped cells have tendency to float. 

Culture Medium

DMEM + 4mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent/aggregates in suspension

Additional Info

Depositor

Dr C Helson, St Agnes Hospital, New York

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Natl Cancer Inst 1984;73(1):51; Anti Cancer Drugs 1993;4:487

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: SK-N-F1 (ECACC 94092304).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.