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RPMI 6666

RPMI 6666

Catalogue No.


Cell Line Name

RPMI 6666

Cell Line Description

Derived from a 29 year old male Caucasian with Hodgkin's disease. One of a large series of permanent lines from leukocytes in the peripheral blood of donors with and without malignancies. Has cell-associated EBV and produces immunoglobulins. This cell line was initially thought to have been derived from Hodgkin lymphoma, but is now known to be an EBV-positive B-lymphoblastoid cell line (Drexler et al., 2003).




Tissue of Origin


DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 12,13
D5S818: 11,12
D7S820: 9,10
D13S317: 11
D16S539: 11,12
TH01: 6,9
TPOX: 10,11
vWA: 14,18


2n = 46


Tumourigenicity studies


Hodgkins Disease

Culture Conditions

Cell Type


Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2



New York State J Med 1966;66:2757, Drexler HG, Dirks WG, Matsuo Y, MacLeod RA. False leukemia-lymphoma cell lines: an update on over 500 cell lines. Leukemia 2003 Feb;17(2):416-26. PMID:12592342


Not specified

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: RPMI 6666 (ECACC 87081807).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.