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PE/CA-PJ49

PE/CA-PJ49

Catalogue No.

00060606

Cell Line Name

PE/CA-PJ49

Cell Line Description

The squamous carcinoma cell line PE/CA-PJ49 has been obtained from a caucasian male of 57 years old.

Characteristics

Tissue of Origin

Tongue

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,12
D5S818: 8,12
D7S820: 8,9
D13S317: 8,11
D16S539: 11
TH01: 6,10
TPOX: 8
vWA: 16,19

Karyotype

Not specified

Applications

The cells can be used in in vitro invasion models for oral squamous epithelial carcinomas to evaluate turnaround stromal cell properties, as well as extra-cellular matrix.

Disease

Squamous Cell Carcinoma

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4-1:8 ie. seeding at 1-3 x10,000 cells/cm² using 0.05% Tyrpsin/EDTA, 37°C, 5% CO₂

Culture Medium

IDMEM + 10%Foetal Bovine Serum (FBS).+ 2mM Glutamine.

Growth Mode

Adherent

Additional Info

Depositor

Dr A Berndt and Dr H Kosmehl. Fredrich-Schiller University, Institute of Pathology, Ziegelmühlenweg 1, D-07740, Jena

Country of Origin

Germany

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

None specified by depositor

Bibliography

Barretina J, et al., 2012 The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 483(7391):603-7. PMID: 22460905.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: PE/CA-PJ49 (ECACC 00060606).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.