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Catalogue No.


Cell Line Name


Cell Line Description

ND3 hybrid cells were derived after fusion of primary neonatal-rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)-derived azaguanine-resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. The cells express all isoforms of N-CAM but no embryonic form. Thy 1.1 marker, P31 and PrPc can be detected as well as a weaker expression of F3 and Thy-3.


Tissue of Origin



Not specified


Study of neuronal differentiation, cell adhesion and expression of sensory neuron specific marker


Neuroblastoma Hybrid

Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Dr J N Wood, Neuroimmunology Dept, The Sandoz Institute, London

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2



Proc Royal Soc (B) 1990;241:187


Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: ND3 (ECACC 92090901).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.