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Catalogue No.


Cell Line Name


Cell Line Description

NCI-H69 VCR/R has been derived from the parent small cell lung cancer line NCI-H69 (ECACC catalogue no. 91091802) It is recommended to culture cells without drug upon resuscitation; add vincristine once growth is fully established. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.


Tissue of Origin


DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 12
D5S818: 11
D7S820: 9
D13S317: 12
D16S539: 11
TH01: 8,9
TPOX: 10
vWA: 16,17


Not specified


Drug-resistance studies


Small Cell Cancer

Culture Conditions

Subculture Routine

Maintain cultures between 3-9x 100,000 cells/ml; 5% CO₂; 37ºC. Resuscitate in drug-free medium, add after growth has been established.

Culture Medium

RPMI 1640 + 2mM Glutamine + 2.0 µg/ml vincristine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Aggregates in suspension

Additional Info


Dr P Twentyman, Dr. K. Wright, UKCCR, PO Box 123, Lincolns Inn Fields, London WC2A 3PX

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2



None specified by depositor

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: NCI-H69VCR/R (ECACC 96042330).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.